Q6EMK4 (VASN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vasorin

Alternative name(s):
Protein slit-like 2

Gene names

Name:	VASN
Synonyms:	SLITL2
ORF Names:	UNQ314/PRO357/PRO1282

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	673 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as an inhibitor of TGF-beta signaling. Ref.1
Subunit structure	Interacts with TGFB1, TGFB2 and TGFB3. Ref.1
Subcellular location	Membrane; Single-pass type I membrane protein Probable. Secreted Ref.1.
Tissue specificity	Expressed at highest levels in aorta, at intermediate levels in kidney and placenta and at lowest levels in brain, heart, liver, lung and skeletal muscle. Within the aorta, the strongest expression is found in the tunica media of the proximal ascending aorta, the descending thoracic aorta, the abdominal aorta and the coronary arteries. Within the kidney, expression is found in the interstitial cells. Ref.1
Post-translational modification	N-glycosylated. Ref.1 Ref.4 Ref.5
Sequence similarities	Contains 1 EGF-like domain. Contains 1 fibronectin type-III domain. Contains 10 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain.

Ontologies

Keywords
Cellular component	Membrane Secreted
Coding sequence diversity	Polymorphism
Domain	EGF-like domain Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 673	650	Vasorin		PRO_0000232630
Regions
	Topological domain	24 – 575	552	Extracellular Potential
	Transmembrane	576 – 596	21	Helical; Potential
	Topological domain	597 – 673	77	Cytoplasmic Potential
	Domain	24 – 51	28	LRRNT
	Repeat	52 – 74	23	LRR 1
	Repeat	77 – 98	22	LRR 2
	Repeat	101 – 122	22	LRR 3
	Repeat	125 – 146	22	LRR 4
	Repeat	149 – 170	22	LRR 5
	Repeat	171 – 191	21	LRR 6
	Repeat	193 – 214	22	LRR 7
	Repeat	217 – 238	22	LRR 8
	Repeat	240 – 262	23	LRR 9
	Repeat	265 – 287	23	LRR 10
	Domain	298 – 351	54	LRRCT
	Domain	405 – 442	38	EGF-like
	Domain	458 – 555	98	Fibronectin type-III
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	117	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	273	1	N-linked (GlcNAc...) Ref.4 Ref.5
	Glycosylation	500	1	N-linked (GlcNAc...) Potential
	Glycosylation	528	1	N-linked (GlcNAc...) Potential
	Disulfide bond	409 ↔ 420		By similarity
	Disulfide bond	414 ↔ 430		By similarity
	Disulfide bond	432 ↔ 441		By similarity
Natural variations
	Natural variant	384	1	E → A. Ref.2 Ref.3 Corresponds to variant rs3810818 [ dbSNP | Ensembl ].		VAR_025991
Experimental info
	Sequence conflict	70	1	G → S in AAQ88665. Ref.2
	Sequence conflict	91 – 165	75	Missing in AAQ88665. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6EMK4 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 891E149652DEA286	FASTA	673	71,713
	10 20 30 40 50 60 MCSRVPLLLP LLLLLALGPG VQGCPSGCQC SQPQTVFCTA RQGTTVPRDV PPDTVGLYVF 70 80 90 100 110 120 ENGITMLDAG SFAGLPGLQL LDLSQNQIAS LPSGVFQPLA NLSNLDLTAN RLHEITNETF 130 140 150 160 170 180 RGLRRLERLY LGKNRIRHIQ PGAFDTLDRL LELKLQDNEL RALPPLRLPR LLLLDLSHNS 190 200 210 220 230 240 LLALEPGILD TANVEALRLA GLGLQQLDEG LFSRLRNLHD LDVSDNQLER VPPVIRGLRG 250 260 270 280 290 300 LTRLRLAGNT RIAQLRPEDL AGLAALQELD VSNLSLQALP GDLSGLFPRL RLLAAARNPF 310 320 330 340 350 360 NCVCPLSWFG PWVRESHVTL ASPEETRCHF PPKNAGRLLL ELDYADFGCP ATTTTATVPT 370 380 390 400 410 420 TRPVVREPTA LSSSLAPTWL SPTEPATEAP SPPSTAPPTV GPVPQPQDCP PSTCLNGGTC 430 440 450 460 470 480 HLGTRHHLAC LCPEGFTGLY CESQMGQGTR PSPTPVTPRP PRSLTLGIEP VSPTSLRVGL 490 500 510 520 530 540 QRYLQGSSVQ LRSLRLTYRN LSGPDKRLVT LRLPASLAEY TVTQLRPNAT YSVCVMPLGP 550 560 570 580 590 600 GRVPEGEEAC GEAHTPPAVH SNHAPVTQAR EGNLPLLIAP ALAAVLLAAL AAVGAAYCVR 610 620 630 640 650 660 RGRAMAAAAQ DKGQVGPGAG PLELEGVKVP LEPGPKATEG GGEALPSGSE CEVPLMGFPG 670 PGLQSPLHAK PYI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Vasorin, a transforming growth factor beta-binding protein expressed in vascular smooth muscle cells, modulates the arterial response to injury in vivo." Ikeda Y., Imai Y., Kumagai H., Nosaka T., Morikawa Y., Hisaoka T., Manabe I., Maemura K., Nakaoka T., Imamura T., Miyazono K., Komuro I., Nagai R., Kitamura T. Proc. Natl. Acad. Sci. U.S.A. 101:10732-10737(2004) [PubMed: 15247411] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TGFB1; TGFB2 AND TGFB3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-384.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-384. Tissue: Lung and Placenta.
[4]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-117 AND ASN-273, MASS SPECTROMETRY. Tissue: Plasma.
[5]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-101 AND ASN-273, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY166584 mRNA. Translation: AAO27704.1. AY358298 mRNA. Translation: AAQ88665.1. AY358299 mRNA. Translation: AAQ88666.1. BC013767 mRNA. Translation: AAH13767.1. BC068575 mRNA. Translation: AAH68575.1.
IPI	IPI00395488.
RefSeq	NP_612449.2. NM_138440.2.
UniGene	Hs.372579.
3D structure databases
ProteinModelPortal	Q6EMK4.
SMR	Q6EMK4. Positions 22-356, 463-541.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46245N.
STRING	Q6EMK4.
PTM databases
PhosphoSite	Q6EMK4.
Polymorphism databases
DMDM	74748436.
Proteomic databases
PeptideAtlas	Q6EMK4.
PRIDE	Q6EMK4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000304735; ENSP00000306864; ENSG00000168140.
GeneID	114990.
KEGG	hsa:114990.
NMPDR	fig|9606.3.peg.11656.
UCSC	uc002cwj.1. human.
Organism-specific databases
CTD	114990.
GeneCards	GC16P004421.
H-InvDB	HIX0038582.
HGNC	HGNC:18517. VASN.
HPA	HPA011246.
MIM	608843. gene.
neXtProt	NX_Q6EMK4.
PharmGKB	PA134974883.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG08775.
GeneTree	ENSGT00600000084168.
HOGENOM	HBG714297.
HOVERGEN	HBG084318.
InParanoid	Q6EMK4.
OMA	HAPVTQA.
OrthoDB	EOG4GTKD4.
PhylomeDB	Q6EMK4.
Gene expression databases
ArrayExpress	Q6EMK4.
Bgee	Q6EMK4.
CleanEx	HS_VASN.
Genevestigator	Q6EMK4.
GermOnline	ENSG00000168140. Homo sapiens.
Family and domain databases
InterPro	IPR000483. Cys-rich_flank_reg_C. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000742. EGF_3. IPR003961. Fibronectin_type3. IPR013783. Ig-like_fold. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000372. LRR-contain_N. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00560. LRR_1. 1 hit. PF01462. LRRNT. 1 hit. [Graphical view]
SMART	SM00181. EGF. 1 hit. SM00060. FN3. 1 hit. SM00369. LRR_TYP. 2 hits. SM00082. LRRCT. 1 hit. SM00013. LRRNT. 1 hit. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS50853. FN3. 1 hit. PS51450. LRR. 7 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	79480.
SOURCE	Search...

Entry information

Entry name

VASN_HUMAN

Accession

Primary (citable) accession number: Q6EMK4
Secondary accession number(s): Q6UXL4, Q6UXL5, Q96CX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.