Reviewed, UniProtKB/Swiss-Prot Q6EIC1 (TPMT_FELCA)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:

TPMT

Organism

Felis catus (Cat) (Felis silvestris catus)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine By similarity.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Thiopurine S-methyltransferase		PRO_0000220099
Sites
	Binding site	33	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	152	1	S-adenosyl-L-methionine By similarity
Amino acid modifications
	Modified residue	58	1	N6-acetyllysine By similarity
Natural variations
	Natural variant	7	1	L → S
	Natural variant	8	1	T → I
	Natural variant	15	1	D → N Reduced activity. Ref.1
	Natural variant	113	1	M → L
	Natural variant	233	1	D → V Reduced activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6EIC1-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 56A82A18F02CE113	FASTA	245	28,321
	10 20 30 40 50 60 MDDTSTLTDV KEYPDTEVQK NRVLTLEEWR EKWVDGKIGF HQEQGHQLLK KHLDTFLKGE 70 80 90 100 110 120 NVLRVFFPLC GKAVEMKWFA DRGHCVVGVE ISELGIREFF TEQNLSYSEE PIMEIPGAKV 130 140 150 160 170 180 FKSSSGNISL YCCNLFDLPR VNIGKFDRIW DRGALVAVNP GDRKCYTDIM LSLTRKGFRY 190 200 210 220 230 240 LLAVLSYDPT KHPGPPFYVP DAEIKNLFGS TCNIHCLEKV DVFEERHKSW GIDYIVEKLY LLTEK

References

[1]

"Cat red blood cell thiopurine S-methyltransferase: companion animal pharmacogenetics." Salavaggione O.E., Yang C., Kidd L.B., Thomae B.A., Pankratz V.S., Trepanier L.A., Weinshilboum R.M. J. Pharmacol. Exp. Ther. 308:617-626(2004) [PubMed: 14610243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS SER-7; ILE-8; ASN-15; LEU-113 AND VAL-233.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY324659 mRNA. Translation: AAP79318.1. AY324667 , AY324660, AY324661, AY324662, AY324664, AY324666, AY324665, AY324663 Genomic DNA. Translation: AAP79305.1.
RefSeq	NP_001009836.1.
3D structure databases
SMR	Q6EIC1. Positions 17-245.
ModBase	Search...
Genome annotation databases
GeneID	493759.
Organism-specific databases
CTD	493759.
Phylogenomic databases
eggNOG	maNOG10315.
HOVERGEN	Q6EIC1.
Enzyme and pathway databases
BRENDA	2.1.1.67. 273949.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_FELCA

Accession

Primary (citable) accession number: Q6EIC1
Secondary accession number(s): Q6EIC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)