Reviewed, UniProtKB/Swiss-Prot Q6EH50 (RIP4_PHYHE)

Names and origin

Protein names

Recommended name:
    Heterotepalin-4
    EC=3.2.2.22
Alternative name(s):
    Ribosome-inactivating protein
    rRNA N-glycosidase

Gene names

Name:

RIP1

Organism

Phytolacca heterotepala (Mexican pokeweed)

Taxonomic identifier

248308 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Phytolaccaceae › Phytolacca

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits protein synthesis in vitro. Ref.2
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.2
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.
Mass spectrometry	Molecular mass is 29326 Da from positions 23 - 283. Determined by ESI. Ref.2

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Ref.2 Inferred from direct assay. Source: UniProtKB pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.2
	Chain	23 – 283	261	Heterotepalin-4 Ref.2		PRO_0000352792
	Propeptide	284 – 312	29	Ref.2		PRO_0000352793
Sites
	Active site	197	1	By similarity UniProtKB P20656
Amino acid modifications
	Disulfide bond	56 ↔ 280		By similarity UniProtKB P10297
	Disulfide bond	107 ↔ 128		By similarity UniProtKB P10297
Experimental info
	Sequence conflict	266	1	I → M in AAQ95991. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q6EH50-1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 4C55B0D9FB8F189B	FASTA	312	35,153
	10 20 30 40 50 60 MKSMLVVTIS VWLILAPTST WAVNTIIYNV GSTTISKYAT FLDDLRNEAK DPNLKCYGIP 70 80 90 100 110 120 MLPNTNSNPK YVLVELQGSN KKTITLMLRR NNLYVMGYSD PFDTSKCRYH IFNDISGTER 130 140 150 160 170 180 QDVETTLCPN SNSRVSKNIN YDSRYPTLES KVGVKSRSQV QLGIQILDSD IGKISGVTSF 190 200 210 220 230 240 SEKTEAEFLL VAIQISEAAR FKYIENQVKT NFNRAFNPNP KVLNLEETWG KISTAIHDAK 250 260 270 280 290 300 NGVLPKPLEL VDASGAKWIV LRVDEIKPDV ALLNYVSGSC QTTYNQNAMF PQLIMSTYYN 310 YMANLGDLFE EF

References

[1]

Delli Bovi P., Corrado G. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

[2]

"Isolation and characterization of heterotepalins, type 1 ribosome-inactivating proteins from Phytolacca heterotepala leaves." Di Maro A., Chambery A., Daniele A., Casoria P., Parente A. Phytochemistry 68:767-776(2007) [PubMed: 17258249] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-37; 38-46; 71-81; 91-106; 109-120; 145-151; 158-173; 174-183; 203-209; 222-231; 232-240; 247-257 AND 262-283, FUNCTION, CATALYTIC ACTIVITY, MASS SPECTROMETRY. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY327475 mRNA. Translation: AAQ95991.1.
3D structure databases
SMR	Q6EH50. Positions 23-283.
ModBase	Search...
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. IPR016331. Shiga-like_toxin_subunit_A. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PIRSF	PIRSF001924. Shigella_toxin_subunit_A. 1 hit.
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP4_PHYHE

Accession

Primary (citable) accession number: Q6EH50

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 14, 2008
Last modified:	March 3, 2009
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)