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Q6EEV6 (SUMO4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Small ubiquitin-related modifier 4

Short name=SUMO-4
Alternative name(s):
Small ubiquitin-like protein 4
Gene names
Name:SUMO4
Synonyms:SMT3H4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length95 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. Ref.1 Ref.2 Ref.9

Subunit structure

Interacts with SAE2. Covalently attached to a number of proteins Probable.

Tissue specificity

Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen. Ref.1 Ref.2

Post-translational modification

In contrast to SUMO1, SUMO2 and SUMO3, seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates.

Polymorphism

Variant Val-55 could be associated with insulin-dependent diabetes mellitus 5 (IDDM5) [MIM:600320].

Sequence similarities

Belongs to the ubiquitin family. SUMO subfamily.

Contains 1 ubiquitin-like domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityPolymorphism
   PTMIsopeptide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein sumoylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9393Small ubiquitin-related modifier 4
PRO_0000042710
Propeptide94 – 952 By similarity
PRO_0000042711

Regions

Domain17 – 9579Ubiquitin-like

Amino acid modifications

Cross-link93Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Natural variations

Natural variant551M → V May be associated with susceptibility to type 1 diabetes; greater NFKB1 transcriptional activity and IL12B expression. Ref.1 Ref.2 Ref.4 Ref.5 Ref.6 Ref.7 Ref.10 Ref.12 Ref.13
Corresponds to variant rs237025 [ dbSNP | Ensembl ].
VAR_023740

Sequences

Sequence LengthMass (Da)Tools
Q6EEV6 [UniParc].

Last modified November 8, 2005. Version 2.
Checksum: 9012CAEE734ED223

FASTA9510,685
        10         20         30         40         50         60 
MANEKPTEEV KTENNNHINL KVAGQDGSVV QFKIKRQTPL SKLMKAYCEP RGLSMKQIRF 

        70         80         90 
RFGGQPISGT DKPAQLEMED EDTIDVFQQP TGGVY 

« Hide

References

« Hide 'large scale' references
[1]"A M55V polymorphism in a novel SUMO gene (SUMO-4) differentially activates heat shock transcription factors and is associated with susceptibility to type I diabetes mellitus."
Bohren K.M., Nadkarni V., Song J.H., Gabbay K.H., Owerbach D.
J. Biol. Chem. 279:27233-27238(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANT VAL-55, CHARACTERIZATION OF VARIANT VAL-55.
[2]"A functional variant of SUMO4, a new I kappa B alpha modifier, is associated with type 1 diabetes."
Guo D., Li M., Zhang Y., Yang P., Eckenrode S., Hopkins D., Zheng W., Purohit S., Podolsky R.H., Muir A., Wang J., Dong Z., Brusko T., Atkinson M., Pozzilli P., Zeidler A., Raffel L.J., Jacob C.O. expand/collapse author list , Park Y., Serrano-Rios M., Martinez Larrad M.T., Zhang Z., Garchon H.-J., Bach J.-F., Rotter J.I., She J.-X., Wang C.-Y.
Nat. Genet. 36:837-841(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, VARIANT VAL-55, CHARACTERIZATION OF VARIANT VAL-55.
[3]Erratum
Guo D., Li M., Zhang Y., Yang P., Eckenrode S., Hopkins D., Zheng W., Purohit S., Podolsky R.H., Muir A., Wang J., Dong Z., Brusko T., Atkinson M., Pozzilli P., Zeidler A., Raffel L.J., Jacob C.O. expand/collapse author list , Park Y., Serrano-Rios M., Martinez Larrad M.T., Zhang Z., Garchon H.-J., Bach J.-F., Rotter J.I., She J.-X., Wang C.-Y.
Nat. Genet. 36:1024-1024(2004)
[4]"SUMO4."
Noso S.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-55.
[5]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-55.
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-55.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-55.
[8]"A proline-90 residue unique to SUMO-4 prevents maturation and sumoylation."
Owerbach D., McKay E.M., Yeh E.T.H., Gabbay K.H., Bohren K.M.
Biochem. Biophys. Res. Commun. 337:517-520(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: LACK OF PROTEOLYTIC CLEAVAGE.
[9]"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress."
Guo D., Han J., Adam B.-L., Colburn N.H., Wang M.-H., Dong Z., Eizirik D.L., She J.-X., Wang C.-Y.
Biochem. Biophys. Res. Commun. 337:1308-1318(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Assessing the validity of the association between the SUMO4 M55V variant and risk of type 1 diabetes."
Smyth D.J., Howson J.M.M., Lowe C.E., Walker N.M., Lam A.C., Nutland S., Hutchings J., Tuomilehto-Wolf E., Tuomilehto J., Guja C., Ionescu-Tirgoviste C., Undlien D.E., Roenningen K.S., Savage D., Dunger D.B., Twells R.C.J., McArdle W.L., Strachan D.P., Todd J.A.
Nat. Genet. 37:110-111(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION OF VARIANT VAL-55 WITH INSULIN-DEPENDENT DIABETES MELLITUS.
[11]Erratum
Smyth D.J., Howson J.M.M., Lowe C.E., Walker N.M., Lam A.C., Nutland S., Hutchings J., Tuomilehto-Wolf E., Tuomilehto J., Guja C., Ionescu-Tirgoviste C., Undlien D.E., Roenningen K.S., Savage D., Dunger D.B., Twells R.C.J., McArdle W.L., Strachan D.P., Todd J.A.
Nat. Genet. 37:328-328(2004)
[12]"Assessing the validity of the association between the SUMO4 M55V variant and risk of type 1 diabetes."
Qu H., Bharaj B., Liu X.Q., Curtis J.A., Newhook L.A., Paterson A.D., Hudson T.J., Polychronakos C.
Nat. Genet. 37:111-112(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION OF VARIANT VAL-55 WITH INSULIN-DEPENDENT DIABETES MELLITUS.
[13]"Assessing the validity of the association between the SUMO4 M55V variant and risk of type 1 diabetes."
Park Y., Park S., Kang J., Yang S., Kim D.
Nat. Genet. 37:112-112(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION OF VARIANT VAL-55 WITH INSULIN-DEPENDENT DIABETES MELLITUS.
+Additional computationally mapped references.

Web resources

Wikipedia

SUMO protein entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY340238 mRNA. Translation: AAR04484.1.
AB205057 Genomic DNA. Translation: BAH05006.1.
AL031133 Genomic DNA. Translation: CAA20019.1.
CH471051 Genomic DNA. Translation: EAW47808.1.
BC130305 mRNA. Translation: AAI30306.1.
CCDSCCDS34549.1.
RefSeqNP_001002255.1. NM_001002255.1.
UniGeneHs.657168.

3D structure databases

ProteinModelPortalQ6EEV6.
SMRQ6EEV6. Positions 17-88.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid132223. 96 interactions.
STRING9606.ENSP00000318635.

PTM databases

PhosphoSiteQ6EEV6.

Polymorphism databases

DMDM81175019.

Proteomic databases

MaxQBQ6EEV6.
PaxDbQ6EEV6.
PRIDEQ6EEV6.

Protocols and materials databases

DNASU387082.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000326669; ENSP00000318635; ENSG00000177688.
GeneID387082.
KEGGhsa:387082.
UCSCuc003qml.3. human.

Organism-specific databases

CTD387082.
GeneCardsGC06P149763.
HGNCHGNC:21181. SUMO4.
MIM600320. phenotype.
608829. gene.
neXtProtNX_Q6EEV6.
PharmGKBPA134979206.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5227.
HOGENOMHOG000207495.
HOVERGENHBG053025.
InParanoidQ6EEV6.
KOK12160.
OrthoDBEOG76X62R.
PhylomeDBQ6EEV6.
TreeFamTF315116.

Gene expression databases

BgeeQ6EEV6.
CleanExHS_SUMO4.
GenevestigatorQ6EEV6.

Family and domain databases

InterProIPR022617. Rad60/SUMO_like.
IPR027218. SUMO_chordates.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERPTHR10562:SF10. PTHR10562:SF10. 1 hit.
PfamPF11976. Rad60-SLD. 1 hit.
[Graphical view]
SMARTSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMSSF54236. SSF54236. 1 hit.
PROSITEPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSUMO4.
GenomeRNAi387082.
NextBio101180.
PROQ6EEV6.
SOURCESearch...

Entry information

Entry nameSUMO4_HUMAN
AccessionPrimary (citable) accession number: Q6EEV6
Secondary accession number(s): A1L3W5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM