Q6ECI4 (ZN470_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 470

Alternative name(s):
Chondrogenesis zinc finger protein 1
Short name=CZF-1

Gene names

Name:	ZNF470
Synonyms:	CZF1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	717 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in transcriptional regulation.
Subcellular location	Nucleus Ref.1.
Tissue specificity	Highly expressed in testis. Very low expression in thymus. No expression in other tissues tested. Expressed in a differentiation stage-specific manner in mesenchymal chondrogenic progenitor cells. Ref.1
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 17 C2H2-type zinc fingers. Contains 1 KRAB domain.
Sequence caution	The sequence BAC85218.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay. Source: HPA
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6ECI4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6ECI4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     63-63: G → GKSIFLLLPPHDSVVFYAIIRISHSFPQHRQFLYVFLHAG
     95-251: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 717	717	Zinc finger protein 470		PRO_0000280413
Regions
	Domain	23 – 94	72	KRAB
	Zinc finger	228 – 250	23	C2H2-type 1
	Zinc finger	256 – 278	23	C2H2-type 2
	Zinc finger	284 – 306	23	C2H2-type 3
	Zinc finger	312 – 334	23	C2H2-type 4
	Zinc finger	340 – 362	23	C2H2-type 5
	Zinc finger	368 – 391	24	C2H2-type 6
	Zinc finger	397 – 419	23	C2H2-type 7
	Zinc finger	425 – 447	23	C2H2-type 8
	Zinc finger	453 – 475	23	C2H2-type 9
	Zinc finger	481 – 503	23	C2H2-type 10
	Zinc finger	509 – 531	23	C2H2-type 11
	Zinc finger	537 – 559	23	C2H2-type 12
	Zinc finger	565 – 587	23	C2H2-type 13
	Zinc finger	593 – 615	23	C2H2-type 14
	Zinc finger	621 – 643	23	C2H2-type 15
	Zinc finger	649 – 671	23	C2H2-type 16
	Zinc finger	677 – 699	23	C2H2-type 17
	Motif	216 – 219	4	Nuclear localization signal Potential
	Motif	385 – 388	4	Nuclear localization signal Potential
Natural variations
	Alternative sequence	63	1	G → GKSIFLLLPPHDSVVFYAII RISHSFPQHRQFLYVFLHAG in isoform 2.		VSP_023660
	Alternative sequence	95 – 251	157	Missing in isoform 2.		VSP_023661
	Natural variant	23	1	V → L. Ref.1 Corresponds to variant rs10421285 [ dbSNP | Ensembl ].		VAR_031143
	Natural variant	254	1	K → R. Corresponds to variant rs3752179 [ dbSNP | Ensembl ].		VAR_031144
	Natural variant	418	1	T → I. Ref.1 Ref.4 Corresponds to variant rs4801177 [ dbSNP | Ensembl ].		VAR_031145
	Natural variant	642	1	I → T. Corresponds to variant rs35077804 [ dbSNP | Ensembl ].		VAR_061950
Experimental info
	Sequence conflict	542	1	C → R in AAS64219. Ref.1
	Sequence conflict	584	1	Q → P in AAS64219. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 30, 2010. Version 3. Checksum: 3CE8F2E9FC5603A3	FASTA	717	82,650
	10 20 30 40 50 60 MKSQEEVEVA GIKLCKAMSL GSVTFTDVAI DFSQDEWEWL NLAQRSLYKK VMLENYRNLV 70 80 90 100 110 120 SVGLCISKPD VISLLEQEKD PWVIKGGMNR GLCPDLECVW VTKSLSLNQD IYEEKLPPAI 130 140 150 160 170 180 IMERLKSYDL ECSTLGKNWK CEDLFERELV NQKTHFRQET ITHIDTLIEK RDHSNKSGTV 190 200 210 220 230 240 FHLNTLSYIK QIFPMEERIF NFHTDKKSLK THSVVKKHKQ DRGEKKLLKC NDCEKIFSKI 250 260 270 280 290 300 STLTLHQRIH TGEKPYECIE CGKAFSQSAH LAQHQRIHTG EKPFECTECG KAFSQNAHLV 310 320 330 340 350 360 QHQRVHTGEK PYQCKQCNKA FSQLAHLAQH QRVHTGEKPY ECIECGKAFS DCSSLAHHRR 370 380 390 400 410 420 IHTGKRPYEC IDCGKAFRQN ASLIRHRRYY HTGEKPFDCI DCGKAFTDHI GLIQHKRTHT 430 440 450 460 470 480 GERPYKCNVC GKAFSHGSSL TVHQRIHTGE KPYECNICEK AFSHRGSLTL HQRVHTGEKP 490 500 510 520 530 540 YECKECGKAF RQSTHLAHHQ RIHTGEKPYE CKECSKTFSQ NAHLAQHQKI HTGEKPYECK 550 560 570 580 590 600 ECGKAFSQIA HLVQHQRVHT GEKPYECIEC GKAFSDGSYL VQHQRLHSGK RPYECLECGK 610 620 630 640 650 660 AFRQRASLIC HQRCHTGEKP YECNVCGKAF SHRKSLTLHQ RIHTGEKPYE CKECSKAFSQ 670 680 690 700 710 VAHLTLHKRI HTGERPYECK ECGKAFRQSV HLAHHQRIHT GESSVILSSA LPYHQVL
	Isoform 2 [UniParc]. Checksum: DEA044259E409522	FASTA	599	68,573
	10 20 30 40 50 60 MKSQEEVEVA GIKLCKAMSL GSVTFTDVAI DFSQDEWEWL NLAQRSLYKK VMLENYRNLV 70 80 90 100 110 120 SVGKSIFLLL PPHDSVVFYA IIRISHSFPQ HRQFLYVFLH AGLCISKPDV ISLLEQEKDP 130 140 150 160 170 180 WVIKGGMNRG LCPGEKPYEC IECGKAFSQS AHLAQHQRIH TGEKPFECTE CGKAFSQNAH 190 200 210 220 230 240 LVQHQRVHTG EKPYQCKQCN KAFSQLAHLA QHQRVHTGEK PYECIECGKA FSDCSSLAHH 250 260 270 280 290 300 RRIHTGKRPY ECIDCGKAFR QNASLIRHRR YYHTGEKPFD CIDCGKAFTD HIGLIQHKRT 310 320 330 340 350 360 HTGERPYKCN VCGKAFSHGS SLTVHQRIHT GEKPYECNIC EKAFSHRGSL TLHQRVHTGE 370 380 390 400 410 420 KPYECKECGK AFRQSTHLAH HQRIHTGEKP YECKECSKTF SQNAHLAQHQ KIHTGEKPYE 430 440 450 460 470 480 CKECGKAFSQ IAHLVQHQRV HTGEKPYECI ECGKAFSDGS YLVQHQRLHS GKRPYECLEC 490 500 510 520 530 540 GKAFRQRASL ICHQRCHTGE KPYECNVCGK AFSHRKSLTL HQRIHTGEKP YECKECSKAF 550 560 570 580 590 SQVAHLTLHK RIHTGERPYE CKECGKAFRQ SVHLAHHQRI HTGESSVILS SALPYHQVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and chondrocyte differentiation stage-specific expression of KRAB zinc-finger protein gene ZNF470." Hering T.M., Kazmi N.H., Huynh T.D., Kollar J., Xu L., Hunyady A.B., Johnstone B. Exp. Cell Res. 299:137-147(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANTS LEU-23 AND ILE-418. Tissue: Mesenchymal stem cell.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 188-717 (ISOFORM 1), VARIANT ILE-418. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY484591 mRNA. Translation: AAS64219.1. AC007228 Genomic DNA. Translation: AAD23607.1. BC136762 mRNA. Translation: AAI36763.1. AK129686 mRNA. Translation: BAC85218.1. Different initiation.
IPI	IPI00294218. IPI00791472.
RefSeq	NP_001001668.3. NM_001001668.3.
UniGene	Hs.204449.
3D structure databases
HSSP	HSSP built from PDB template 1X6E based on UniProtKB P17028.
ProteinModelPortal	Q6ECI4.
SMR	Q6ECI4. Positions 23-63, 207-702.
ModBase	Search...
PTM databases
PhosphoSite	Q6ECI4.
Polymorphism databases
DMDM	134035366.
Proteomic databases
PaxDb	Q6ECI4.
PRIDE	Q6ECI4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000330619; ENSP00000333223; ENSG00000197016. ENST00000391709; ENSP00000375590; ENSG00000197016.
GeneID	388566.
KEGG	hsa:388566.
UCSC	uc002qnl.4. human.
Organism-specific databases
CTD	388566.
GeneCards	GC19P057078.
HGNC	HGNC:22220. ZNF470.
HPA	HPA029000.
neXtProt	NX_Q6ECI4.
PharmGKB	PA145007252.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000234617.
HOVERGEN	HBG018163.
InParanoid	Q6ECI4.
KO	K09228.
OMA	SKAFSQI.
OrthoDB	EOG4T782P.
Gene expression databases
Bgee	Q6ECI4.
CleanEx	HS_ZNF470.
Genevestigator	Q6ECI4.
Family and domain databases
Gene3D	3.30.160.60. 17 hits.
InterPro	IPR001909. Krueppel-associated_box. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF01352. KRAB. 1 hit. [Graphical view]
SMART	SM00349. KRAB. 1 hit. SM00355. ZnF_C2H2. 17 hits. [Graphical view]
SUPFAM	SSF109640. Krueppel-associated_box. 1 hit.
PROSITE	PS50805. KRAB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 17 hits. PS50157. ZINC_FINGER_C2H2_2. 17 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	388566.
NextBio	102168.

Entry information

Entry name

ZN470_HUMAN

Accession

Primary (citable) accession number: Q6ECI4
Secondary accession number(s): A8MTW0 , B9EGU1, Q6ZPA1, Q9Y2N9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.