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Protein

Autophagy-related protein 9B

Gene

Atg9b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 9B
Alternative name(s):
APG9-like 2
Nitric oxide synthase 3-overlapping antisense gene protein
Gene namesi
Name:Atg9b
Synonyms:Apg9l2, Nos3as
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2685420. Atg9b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 206206CytoplasmicBy similarityAdd
BLAST
Transmembranei207 – 22721HelicalSequence analysisAdd
BLAST
Topological domaini228 – 27548LumenalBy similarityAdd
BLAST
Transmembranei276 – 29621HelicalSequence analysisAdd
BLAST
Topological domaini297 – 437141CytoplasmicBy similarityAdd
BLAST
Transmembranei438 – 45821HelicalSequence analysisAdd
BLAST
Topological domaini459 – 52365LumenalBy similarityAdd
BLAST
Transmembranei524 – 54421HelicalSequence analysisAdd
BLAST
Topological domaini545 – 5506CytoplasmicBy similarity
Transmembranei551 – 57121HelicalSequence analysisAdd
BLAST
Topological domaini572 – 62453LumenalBy similarityAdd
BLAST
Transmembranei625 – 64521HelicalSequence analysisAdd
BLAST
Topological domaini646 – 922277CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • autophagosome Source: MGI
  • autophagosome membrane Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • cytoplasmic vesicle Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 922922Autophagy-related protein 9BPRO_0000314868Add
BLAST

Proteomic databases

PaxDbiQ6EBV9.
PRIDEiQ6EBV9.

PTM databases

iPTMnetiQ6EBV9.
PhosphoSiteiQ6EBV9.

Expressioni

Tissue specificityi

Expressed in heart, brain, and placenta and testis.2 Publications

Gene expression databases

GenevisibleiQ6EBV9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051864.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi86 – 14661Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the ATG9 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2173. Eukaryota.
ENOG410XQBE. LUCA.
GeneTreeiENSGT00390000014839.
HOGENOMiHOG000015255.
HOVERGENiHBG050539.
InParanoidiQ6EBV9.
KOiK17907.
OMAiGQFIFIV.
OrthoDBiEOG72C4ZM.
TreeFamiTF313665.

Family and domain databases

InterProiIPR007241. Autophagy-rel_prot_9.
[Graphical view]
PANTHERiPTHR13038. PTHR13038. 1 hit.
PfamiPF04109. APG9. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6EBV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRRTGWGGS RRQRGRWGDL GPSSVPLLPM ALPLPASPCR GTGGRRISVF
60 70 80 90 100
SLSPAPRTRS CSSSVFPPAS GSPCLVIQEA GASQTPHNVL PTPTTPSTQA
110 120 130 140 150
HPTMIHTSAS PSWGSHSTPP LASATPPPSC PRPQDHPGLR MGPLIPEQDY
160 170 180 190 200
ERLEDCDPEG SQDSPIHGED HQPLLHVPEG LRGSWHHIQN LDSFFTKIYS
210 220 230 240 250
YHQRNGFACI LLEDVFQLGQ FIFIVTFTTF LLRCVDYNVL FNNQPKNHTR
260 270 280 290 300
RGPLHSKVTL SDAILPSAQC AEKIHDSPLL VFLLVLAAGF WLFQLLRSVC
310 320 330 340 350
NLFSYWDIQV FYREALHIPP EELSSVPWAE VQSRLLELQR SGGLCVQPRP
360 370 380 390 400
LTELDVHHRI LRYTNYQVAL ANKGLLPARC PLPWGSSAAF LSRGLALNVD
410 420 430 440 450
LLLFRGPFSL FRGGWELPEA YKRSDLRGVL ANRWRRTVLL LAAVNLALSP
460 470 480 490 500
LVLAWQVLHA FYSHVELLRR EPGAFGARRW SRLARLQLRH FNELPHELRA
510 520 530 540 550
RLGRAYRPAA AFLRAAEPPA PLRALLARQL VFFSGALFAA LLVLTIYDED
560 570 580 590 600
VLAVEHVLTT MTALGVTATV ARSFIPEEQC QGRSSQLLLQ AALAHMHYLP
610 620 630 640 650
EEPGATGARA SSYWQMAQLL QYRAVSLLEE LLSPLLTPLF LLFWFRPRAL
660 670 680 690 700
EIIDFFHHFT VDVAGVGDIC SFALMDVKRH GHPQWLSEGQ TEASLSQRAE
710 720 730 740 750
DGKTELSLMR FSLAHPQWQP PGHSSKFLGH LRGRVQQDAA AWGAPSTRSP
760 770 780 790 800
PTPGVLSDCT SPLPEAFLAN LLVNPRPPQR DLSPTAPCPA AATASLLASI
810 820 830 840 850
SRMVQDPSCV SPGGTGGQKL TQLPELVSAE MSLHAIYLHQ LHQQQQQELW
860 870 880 890 900
GEASASSPSR PWSSPSQPGS PDEEKPSWSS DGSSPASSPR QQWGTQRAQN
910 920
LFPKGFQENT DTQKEPLTGP LH
Length:922
Mass (Da):101,913
Last modified:October 3, 2012 - v2
Checksum:i3E79390541C3DB9D
GO

Sequence cautioni

The sequence AAS87213.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291P → H in AAS72555 (PubMed:15234981).Curated
Sequence conflicti29 – 291P → H in AAS87214 (PubMed:15234981).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY495541 mRNA. Translation: AAS72555.1.
AY515312 mRNA. Translation: AAS87213.1. Different initiation.
AY515313 mRNA. Translation: AAS87214.1.
AC113055 Genomic DNA. No translation available.
BK004021 mRNA. Translation: DAA05202.1.
CCDSiCCDS39027.1.
RefSeqiNP_001002897.2. NM_001002897.3.
UniGeneiMm.332252.

Genome annotation databases

EnsembliENSMUST00000059401; ENSMUSP00000051864; ENSMUSG00000038295.
GeneIDi213948.
KEGGimmu:213948.
UCSCiuc033ihy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY495541 mRNA. Translation: AAS72555.1.
AY515312 mRNA. Translation: AAS87213.1. Different initiation.
AY515313 mRNA. Translation: AAS87214.1.
AC113055 Genomic DNA. No translation available.
BK004021 mRNA. Translation: DAA05202.1.
CCDSiCCDS39027.1.
RefSeqiNP_001002897.2. NM_001002897.3.
UniGeneiMm.332252.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000051864.

PTM databases

iPTMnetiQ6EBV9.
PhosphoSiteiQ6EBV9.

Proteomic databases

PaxDbiQ6EBV9.
PRIDEiQ6EBV9.

Protocols and materials databases

DNASUi213948.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059401; ENSMUSP00000051864; ENSMUSG00000038295.
GeneIDi213948.
KEGGimmu:213948.
UCSCiuc033ihy.1. mouse.

Organism-specific databases

CTDi285973.
MGIiMGI:2685420. Atg9b.

Phylogenomic databases

eggNOGiKOG2173. Eukaryota.
ENOG410XQBE. LUCA.
GeneTreeiENSGT00390000014839.
HOGENOMiHOG000015255.
HOVERGENiHBG050539.
InParanoidiQ6EBV9.
KOiK17907.
OMAiGQFIFIV.
OrthoDBiEOG72C4ZM.
TreeFamiTF313665.

Miscellaneous databases

PROiQ6EBV9.
SOURCEiSearch...

Gene expression databases

GenevisibleiQ6EBV9. MM.

Family and domain databases

InterProiIPR007241. Autophagy-rel_prot_9.
[Graphical view]
PANTHERiPTHR13038. PTHR13038. 1 hit.
PfamiPF04109. APG9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Post-transcriptional regulation of endothelial nitric-oxide synthase by an overlapping antisense mRNA transcript."
    Robb G.B., Carson A.R., Tai S.C., Fish J.E., Singh S., Yamada T., Scherer S.W., Nakabayashi K., Marsden P.A.
    J. Biol. Chem. 279:37982-37996(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: 129S6/SvEvTac and CD-1.
    Tissue: Placenta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Endothelial nitric-oxide synthase antisense (NOS3AS) gene encodes an autophagy-related protein (APG9-like2) highly expressed in trophoblast."
    Yamada T., Carson A.R., Caniggia I., Umebayashi K., Yoshimori T., Nakabayashi K., Scherer S.W.
    J. Biol. Chem. 280:18283-18290(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiATG9B_MOUSE
AccessioniPrimary (citable) accession number: Q6EBV9
Secondary accession number(s): F8VQL8, Q674R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 3, 2012
Last modified: June 8, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.