Q6EBV9 (ATG9B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy-related protein 9B

Alternative name(s):
APG9-like 2
Nitric oxide synthase 3-overlapping antisense gene protein

Gene names

Name:	Atg9b
Synonyms:	Apg9l2, Nos3as

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	922 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role in autophagy. Ref.2
Subcellular location	Cytoplasmic vesicle › autophagosome membrane; Multi-pass membrane protein Ref.2.
Tissue specificity	Expressed in heart, brain, and placenta and testis. Ref.1 Ref.2
Sequence similarities	Belongs to the ATG9 family.
Sequence caution	The sequence AAS87213.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Autophagy
Cellular component	Cytoplasmic vesicle Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	autophagic vacuole membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 922	922	Autophagy-related protein 9B		PRO_0000314868
Regions
	Topological domain	1 – 206	206	Cytoplasmic By similarity
	Transmembrane	207 – 227	21	Helical; Potential
	Topological domain	228 – 275	48	Lumenal By similarity
	Transmembrane	276 – 296	21	Helical; Potential
	Topological domain	297 – 437	141	Cytoplasmic By similarity
	Transmembrane	438 – 458	21	Helical; Potential
	Topological domain	459 – 523	65	Lumenal By similarity
	Transmembrane	524 – 544	21	Helical; Potential
	Topological domain	545 – 550	6	Cytoplasmic By similarity
	Transmembrane	551 – 571	21	Helical; Potential
	Topological domain	572 – 624	53	Lumenal By similarity
	Transmembrane	625 – 645	21	Helical; Potential
	Topological domain	646 – 922	277	Cytoplasmic By similarity
	Compositional bias	86 – 146	61	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Q6EBV9 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 450179EDD6C8496A	FASTA	922	101,953
	10 20 30 40 50 60 MVRRTGWGGS RRQRGRWGDL GPSSVPLLHM ALPLPASPCR GTGGRRISVF SLSPAPRTRS 70 80 90 100 110 120 CSSSVFPPAS GSPCLVIQEA GASQTPHNVL PTPTTPSTQA HPTMIHTSAS PSWGSHSTPP 130 140 150 160 170 180 LASATPPPSC PRPQDHPGLR MGPLIPEQDY ERLEDCDPEG SQDSPIHGED HQPLLHVPEG 190 200 210 220 230 240 LRGSWHHIQN LDSFFTKIYS YHQRNGFACI LLEDVFQLGQ FIFIVTFTTF LLRCVDYNVL 250 260 270 280 290 300 FNNQPKNHTR RGPLHSKVTL SDAILPSAQC AEKIHDSPLL VFLLVLAAGF WLFQLLRSVC 310 320 330 340 350 360 NLFSYWDIQV FYREALHIPP EELSSVPWAE VQSRLLELQR SGGLCVQPRP LTELDVHHRI 370 380 390 400 410 420 LRYTNYQVAL ANKGLLPARC PLPWGSSAAF LSRGLALNVD LLLFRGPFSL FRGGWELPEA 430 440 450 460 470 480 YKRSDLRGVL ANRWRRTVLL LAAVNLALSP LVLAWQVLHA FYSHVELLRR EPGAFGARRW 490 500 510 520 530 540 SRLARLQLRH FNELPHELRA RLGRAYRPAA AFLRAAEPPA PLRALLARQL VFFSGALFAA 550 560 570 580 590 600 LLVLTIYDED VLAVEHVLTT MTALGVTATV ARSFIPEEQC QGRSSQLLLQ AALAHMHYLP 610 620 630 640 650 660 EEPGATGARA SSYWQMAQLL QYRAVSLLEE LLSPLLTPLF LLFWFRPRAL EIIDFFHHFT 670 680 690 700 710 720 VDVAGVGDIC SFALMDVKRH GHPQWLSEGQ TEASLSQRAE DGKTELSLMR FSLAHPQWQP 730 740 750 760 770 780 PGHSSKFLGH LRGRVQQDAA AWGAPSTRSP PTPGVLSDCT SPLPEAFLAN LLVNPRPPQR 790 800 810 820 830 840 DLSPTAPCPA AATASLLASI SRMVQDPSCV SPGGTGGQKL TQLPELVSAE MSLHAIYLHQ 850 860 870 880 890 900 LHQQQQQELW GEASASSPSR PWSSPSQPGS PDEEKPSWSS DGSSPASSPR QQWGTQRAQN 910 920 LFPKGFQENT DTQKEPLTGP LH

References

[1]	"Post-transcriptional regulation of endothelial nitric-oxide synthase by an overlapping antisense mRNA transcript." Robb G.B., Carson A.R., Tai S.C., Fish J.E., Singh S., Yamada T., Scherer S.W., Nakabayashi K., Marsden P.A. J. Biol. Chem. 279:37982-37996(2004) [PubMed: 15234981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: 129S6/SvEvTac and CD-1. Tissue: Placenta.
[2]	"Endothelial nitric-oxide synthase antisense (NOS3AS) gene encodes an autophagy-related protein (APG9-like2) highly expressed in trophoblast." Yamada T., Carson A.R., Caniggia I., Umebayashi K., Yoshimori T., Nakabayashi K., Scherer S.W. J. Biol. Chem. 280:18283-18290(2005) [PubMed: 15755735] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY495541 mRNA. Translation: AAS72555.1. AY515312 mRNA. Translation: AAS87213.1. Different initiation. AY515313 mRNA. Translation: AAS87214.1. BK004021 mRNA. Translation: DAA05202.1.
IPI	IPI00268141.
RefSeq	NP_001002897.2. NM_001002897.3.
UniGene	Mm.332252.
3D structure databases
ProteinModelPortal	Q6EBV9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6EBV9.
Proteomic databases
PRIDE	Q6EBV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	213948.
KEGG	mmu:213948.
UCSC	uc008wrf.1. mouse.
Organism-specific databases
CTD	285973.
MGI	MGI:2685420. Atg9b.
Phylogenomic databases
eggNOG	roNOG06068.
GeneTree	ENSGT00390000014839.
HOGENOM	HBG315812.
HOVERGEN	HBG050539.
InParanoid	Q6EBV9.
OrthoDB	EOG4W0XCJ.
PhylomeDB	Q6EBV9.
Gene expression databases
ArrayExpress	Q6EBV9.
Bgee	Q6EBV9.
Genevestigator	Q6EBV9.
Family and domain databases
InterPro	IPR007241. Autophagy-rel_prot_9. [Graphical view]
PANTHER	PTHR13038. APG9. 1 hit.
Pfam	PF04109. APG9. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	374136.
SOURCE	Search...

Entry information

Entry name

ATG9B_MOUSE

Accession

Primary (citable) accession number: Q6EBV9
Secondary accession number(s): Q674R6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 16, 2004
Last modified:	September 21, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program