Reviewed, UniProtKB/Swiss-Prot Q6E4Q0 (DUT_ANTLO)

Names and origin

Protein names

Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase
      Short name=dUTPase
    EC=3.6.1.23
Alternative name(s):
    dUTP pyrophosphatase

Gene names

Name:

DUT1

Organism

Antonospora locustae (Microsporidian parasite) (Nosema locustae)

Taxonomic identifier

278021 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Microsporidia › Antonospora

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Subunit structure	Homotrimer By similarity.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 143	143	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000182927
Regions
	Region	64 – 66	3	Substrate binding By similarity
	Region	78 – 81	4	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Binding site	132	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6E4Q0-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: F8E434A8A47CBA8C	FASTA	143	15,526
	10 20 30 40 50 60 MSEIITVKRL FSDAKIPVRH SEGAAAYDLY AYEDTVVAPN ERKVIATGVR ITVPLSCQGT 70 80 90 100 110 120 IYSRSGLALK YCIEIFGVNI GPGETKDIVV DIYNHGKMPF NVAKGDRIAQ IVFIKLFGGD 130 140 LHEVSELSDT KRGSCGWGST GIS

References

[1]	"Genome compaction and stability in microsporidian intracellular parasites." Slamovits C.H., Fast N.M., Law J.S., Keeling P.J. Curr. Biol. 14:891-896(2004) [PubMed: 15186746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY574349 Genomic DNA. Translation: AAT72741.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.23. 288911.
Family and domain databases
InterPro	IPR008180. DeoxyUTP_pyroPase_dom. IPR008181. dUTP_pyrophosphatase_subfam_1. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProDom	PD004900. dCTP_deaminase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_ANTLO

Accession

Primary (citable) accession number: Q6E4Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	August 16, 2004
Last modified:	September 22, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)