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Q6E213 (AWAT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acyl-CoA wax alcohol acyltransferase 2

EC=2.3.1.75
Alternative name(s):
Acyl-CoA retinol O-fatty-acyltransferase
Short name=ARAT
Short name=Retinol O-fatty-acyltransferase
EC=2.3.1.76
Diacylglycerol O-acyltransferase 2-like protein 4
Diacylglycerol O-acyltransferase candidate 4
Short name=hDC4
Long-chain-alcohol O-fatty-acyltransferase 2
Multifunctional O-acyltransferase
Wax synthase
Short name=hWS
Gene names
Name:AWAT2
Synonyms:DC4, DGAT2L4, MFAT, WS
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length333 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has no activity using decyl alcohol and significantly prefers the C16 and C18 alcohols. May also have 2-acylglycerol O-acyltransferase (MGAT) and acyl-CoA:retinol acyltransferase (ARAT) activities, to catalyze the synthesis of diacylglycerols and retinyl esters; however this activity is unclear in vivo. Ref.1 Ref.4 Ref.5

Catalytic activity

Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester. Ref.5

Acyl-CoA + retinol = CoA + retinyl ester. Ref.5

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Abundant in tissues rich in sebaceous glands such as the preputial gland and eyelid. Highly expressed in skin, where it is primarily restricted to undifferentiated peripheral sebocytes. Also expressed at lower level in other tissues except pancreas. Ref.1 Ref.4 Ref.5

Sequence similarities

Belongs to the diacylglycerol acyltransferase family.

Biophysicochemical properties

Kinetic parameters:

Vmax=1232 pmol/min/mg enzyme with [14C]cetyl-CoA as substrate Ref.4

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 333333Acyl-CoA wax alcohol acyltransferase 2
PRO_0000249052

Regions

Transmembrane15 – 3521Helical; Potential
Transmembrane38 – 5821Helical; Potential
Transmembrane130 – 15021Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q6E213 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: B2F1BAAF331BAE4C

FASTA33338,094
        10         20         30         40         50         60 
MLLPSKKDLK TALDVFAVFQ WSFSALLITT TVIAVNLYLV VFTPYWPVTV LILTWLAFDW 

        70         80         90        100        110        120 
KTPQRGGRRF TCVRHWRLWK HYSDYFPLKL LKTHDICPSR NYILVCHPHG LFAHGWFGHF 

       130        140        150        160        170        180 
ATEASGFSKI FPGITPYILT LGAFFWMPFL REYVMSTGAC SVSRSSIDFL LTHKGTGNMV 

       190        200        210        220        230        240 
IVVIGGLAEC RYSLPGSSTL VLKNRSGFVR MALQHGVPLI PAYAFGETDL YDQHIFTPGG 

       250        260        270        280        290        300 
FVNRFQKWFQ SMVHIYPCAF YGRGFTKNSW GLLPYSRPVT TIVGEPLPMP KIENPSQEIV 

       310        320        330 
AKYHTLYIDA LRKLFDQHKT KFGISETQEL EII 

« Hide

References

« Hide 'large scale' references
[1]"Mammalian wax biosynthesis. II. Expression cloning of wax synthase cDNAs encoding a member of the acyltransferase enzyme family."
Cheng J.B., Russell D.W.
J. Biol. Chem. 279:37798-37807(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 176-333.
Tissue: Skin.
[3]"Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals."
Winter A., van Eckeveld M., Bininda-Emonds O.R.P., Habermann F.A., Fries R.
Cytogenet. Genome Res. 102:42-47(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[4]"Identification of two novel human Acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily."
Turkish A.R., Henneberry A.L., Cromley D., Padamsee M., Oelkers P., Bazzi H., Christiano A.M., Billheimer J.T., Sturley S.L.
J. Biol. Chem. 280:14755-14764(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[5]"A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters."
Yen C.-L.E., Brown C.H. IV, Monetti M., Farese R.V. Jr.
J. Lipid Res. 46:2388-2397(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY605053 mRNA. Translation: AAT68764.1.
BC063698 mRNA. Translation: AAH63698.1.
BN000156 mRNA. Translation: CAD89267.1.
RefSeqNP_001002254.1. NM_001002254.1.
UniGeneHs.661507.

3D structure databases

ProteinModelPortalQ6E213.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ6E213.

Polymorphism databases

DMDM74748433.

Proteomic databases

PRIDEQ6E213.

Protocols and materials databases

DNASU158835.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000276101; ENSP00000421172; ENSG00000147160.
GeneID158835.
KEGGhsa:158835.
UCSCuc004dxt.1. human.

Organism-specific databases

CTD158835.
GeneCardsGC0XM069178.
HGNCHGNC:23251. AWAT2.
neXtProtNX_Q6E213.
PharmGKBPA164716410.
GenAtlasSearch...

Phylogenomic databases

HOGENOMHOG000179738.
HOVERGENHBG065791.
InParanoidQ6E213.
KOK11156.
OMACAFYGRG.
OrthoDBEOG7KH9KB.
PhylomeDBQ6E213.
TreeFamTF314707.

Enzyme and pathway databases

BRENDA2.3.1.75. 2681.

Gene expression databases

BgeeQ6E213.
CleanExHS_DGAT2L4.
GenevestigatorQ6E213.

Family and domain databases

InterProIPR007130. DAGAT.
[Graphical view]
PANTHERPTHR12317. PTHR12317. 1 hit.
PfamPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi158835.
NextBio87830.
PROQ6E213.

Entry information

Entry nameAWAT2_HUMAN
AccessionPrimary (citable) accession number: Q6E213
Secondary accession number(s): Q6IEE3, Q6P437
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM