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Q6E213

- AWAT2_HUMAN

UniProt

Q6E213 - AWAT2_HUMAN

Protein

Acyl-CoA wax alcohol acyltransferase 2

Gene

AWAT2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has no activity using decyl alcohol and significantly prefers the C16 and C18 alcohols. May also have 2-acylglycerol O-acyltransferase (MGAT) and acyl-CoA:retinol acyltransferase (ARAT) activities, to catalyze the synthesis of diacylglycerols and retinyl esters; however this activity is unclear in vivo.3 Publications

    Catalytic activityi

    Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.1 Publication
    Acyl-CoA + retinol = CoA + retinyl ester.1 Publication

    Kineticsi

      Vmax=1232 pmol/min/mg enzyme with [14C]cetyl-CoA as substrate1 Publication

      GO - Molecular functioni

      1. long-chain-alcohol O-fatty-acyltransferase activity Source: UniProtKB-EC
      2. retinol O-fatty-acyltransferase activity Source: UniProtKB-EC

      GO - Biological processi

      1. wax biosynthetic process Source: Ensembl

      Keywords - Molecular functioni

      Acyltransferase, Transferase

      Keywords - Biological processi

      Lipid biosynthesis, Lipid metabolism

      Enzyme and pathway databases

      BRENDAi2.3.1.75. 2681.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Acyl-CoA wax alcohol acyltransferase 2 (EC:2.3.1.75)
      Alternative name(s):
      Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
      Short name:
      ARAT
      Short name:
      Retinol O-fatty-acyltransferase
      Diacylglycerol O-acyltransferase 2-like protein 4
      Diacylglycerol O-acyltransferase candidate 4
      Short name:
      hDC4
      Long-chain-alcohol O-fatty-acyltransferase 2
      Multifunctional O-acyltransferase
      Wax synthase
      Short name:
      hWS
      Gene namesi
      Name:AWAT2
      Synonyms:DC4, DGAT2L4, MFAT, WS
      OrganismiHomo sapiens (Human)
      Taxonomic identifieri9606 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
      ProteomesiUP000005640: Chromosome X

      Organism-specific databases

      HGNCiHGNC:23251. AWAT2.

      Subcellular locationi

      Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

      GO - Cellular componenti

      1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
      2. integral component of membrane Source: UniProtKB-KW

      Keywords - Cellular componenti

      Endoplasmic reticulum, Membrane

      Pathology & Biotechi

      Organism-specific databases

      PharmGKBiPA164716410.

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 333333Acyl-CoA wax alcohol acyltransferase 2PRO_0000249052Add
      BLAST

      Proteomic databases

      PRIDEiQ6E213.

      PTM databases

      PhosphoSiteiQ6E213.

      Expressioni

      Tissue specificityi

      Abundant in tissues rich in sebaceous glands such as the preputial gland and eyelid. Highly expressed in skin, where it is primarily restricted to undifferentiated peripheral sebocytes. Also expressed at lower level in other tissues except pancreas.3 Publications

      Gene expression databases

      BgeeiQ6E213.
      CleanExiHS_DGAT2L4.
      GenevestigatoriQ6E213.

      Structurei

      3D structure databases

      ProteinModelPortaliQ6E213.
      ModBaseiSearch...
      MobiDBiSearch...

      Transmembrane

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Transmembranei15 – 3521HelicalSequence AnalysisAdd
      BLAST
      Transmembranei38 – 5821HelicalSequence AnalysisAdd
      BLAST
      Transmembranei130 – 15021HelicalSequence AnalysisAdd
      BLAST

      Family & Domainsi

      Sequence similaritiesi

      Keywords - Domaini

      Transmembrane, Transmembrane helix

      Phylogenomic databases

      HOGENOMiHOG000179738.
      HOVERGENiHBG065791.
      InParanoidiQ6E213.
      KOiK11156.
      OMAiCAFYGRG.
      OrthoDBiEOG7KH9KB.
      PhylomeDBiQ6E213.
      TreeFamiTF314707.

      Family and domain databases

      InterProiIPR007130. DAGAT.
      [Graphical view]
      PANTHERiPTHR12317. PTHR12317. 1 hit.
      PfamiPF03982. DAGAT. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      Q6E213-1 [UniParc]FASTAAdd to Basket

      « Hide

      MLLPSKKDLK TALDVFAVFQ WSFSALLITT TVIAVNLYLV VFTPYWPVTV    50
      LILTWLAFDW KTPQRGGRRF TCVRHWRLWK HYSDYFPLKL LKTHDICPSR 100
      NYILVCHPHG LFAHGWFGHF ATEASGFSKI FPGITPYILT LGAFFWMPFL 150
      REYVMSTGAC SVSRSSIDFL LTHKGTGNMV IVVIGGLAEC RYSLPGSSTL 200
      VLKNRSGFVR MALQHGVPLI PAYAFGETDL YDQHIFTPGG FVNRFQKWFQ 250
      SMVHIYPCAF YGRGFTKNSW GLLPYSRPVT TIVGEPLPMP KIENPSQEIV 300
      AKYHTLYIDA LRKLFDQHKT KFGISETQEL EII 333
      Length:333
      Mass (Da):38,094
      Last modified:August 16, 2004 - v1
      Checksum:iB2F1BAAF331BAE4C
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AY605053 mRNA. Translation: AAT68764.1.
      BC063698 mRNA. Translation: AAH63698.1.
      BN000156 mRNA. Translation: CAD89267.1.
      CCDSiCCDS35320.1.
      RefSeqiNP_001002254.1. NM_001002254.1.
      UniGeneiHs.661507.

      Genome annotation databases

      EnsembliENST00000276101; ENSP00000421172; ENSG00000147160.
      GeneIDi158835.
      KEGGihsa:158835.
      UCSCiuc004dxt.1. human.

      Polymorphism databases

      DMDMi74748433.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AY605053 mRNA. Translation: AAT68764.1 .
      BC063698 mRNA. Translation: AAH63698.1 .
      BN000156 mRNA. Translation: CAD89267.1 .
      CCDSi CCDS35320.1.
      RefSeqi NP_001002254.1. NM_001002254.1.
      UniGenei Hs.661507.

      3D structure databases

      ProteinModelPortali Q6E213.
      ModBasei Search...
      MobiDBi Search...

      Chemistry

      ChEMBLi CHEMBL2375204.

      PTM databases

      PhosphoSitei Q6E213.

      Polymorphism databases

      DMDMi 74748433.

      Proteomic databases

      PRIDEi Q6E213.

      Protocols and materials databases

      DNASUi 158835.
      Structural Biology Knowledgebase Search...

      Genome annotation databases

      Ensembli ENST00000276101 ; ENSP00000421172 ; ENSG00000147160 .
      GeneIDi 158835.
      KEGGi hsa:158835.
      UCSCi uc004dxt.1. human.

      Organism-specific databases

      CTDi 158835.
      GeneCardsi GC0XM069178.
      HGNCi HGNC:23251. AWAT2.
      MIMi 300925. gene.
      neXtProti NX_Q6E213.
      PharmGKBi PA164716410.
      GenAtlasi Search...

      Phylogenomic databases

      HOGENOMi HOG000179738.
      HOVERGENi HBG065791.
      InParanoidi Q6E213.
      KOi K11156.
      OMAi CAFYGRG.
      OrthoDBi EOG7KH9KB.
      PhylomeDBi Q6E213.
      TreeFami TF314707.

      Enzyme and pathway databases

      BRENDAi 2.3.1.75. 2681.

      Miscellaneous databases

      GenomeRNAii 158835.
      NextBioi 87830.
      PROi Q6E213.
      SOURCEi Search...

      Gene expression databases

      Bgeei Q6E213.
      CleanExi HS_DGAT2L4.
      Genevestigatori Q6E213.

      Family and domain databases

      InterProi IPR007130. DAGAT.
      [Graphical view ]
      PANTHERi PTHR12317. PTHR12317. 1 hit.
      Pfami PF03982. DAGAT. 1 hit.
      [Graphical view ]
      ProtoNeti Search...

      Publicationsi

      1. "Mammalian wax biosynthesis. II. Expression cloning of wax synthase cDNAs encoding a member of the acyltransferase enzyme family."
        Cheng J.B., Russell D.W.
        J. Biol. Chem. 279:37798-37807(2004) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
        The MGC Project Team
        Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 176-333.
        Tissue: Skin.
      3. "Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals."
        Winter A., van Eckeveld M., Bininda-Emonds O.R.P., Habermann F.A., Fries R.
        Cytogenet. Genome Res. 102:42-47(2003) [PubMed] [Europe PMC] [Abstract]
        Cited for: IDENTIFICATION.
      4. "Identification of two novel human Acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily."
        Turkish A.R., Henneberry A.L., Cromley D., Padamsee M., Oelkers P., Bazzi H., Christiano A.M., Billheimer J.T., Sturley S.L.
        J. Biol. Chem. 280:14755-14764(2005) [PubMed] [Europe PMC] [Abstract]
        Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      5. "A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters."
        Yen C.-L.E., Brown C.H. IV, Monetti M., Farese R.V. Jr.
        J. Lipid Res. 46:2388-2397(2005) [PubMed] [Europe PMC] [Abstract]
        Cited for: CATALYTIC ACTIVITY, FUNCTION, TISSUE SPECIFICITY.

      Entry informationi

      Entry nameiAWAT2_HUMAN
      AccessioniPrimary (citable) accession number: Q6E213
      Secondary accession number(s): Q6IEE3, Q6P437
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: September 5, 2006
      Last sequence update: August 16, 2004
      Last modified: October 1, 2014
      This is version 84 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programChordata Protein Annotation Program
      DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Reference proteome

      Documents

      1. Human chromosome X
        Human chromosome X: entries, gene names and cross-references to MIM
      2. MIM cross-references
        Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
      3. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3