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Protein

Acyl-CoA wax alcohol acyltransferase 2

Gene

Awat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has no activity using decyl alcohol and significantly prefers the C16 and C18 alcohols. May also have 2-acylglycerol O-acyltransferase (MGAT) and acyl-CoA:retinol acyltransferase (ARAT) activities, to catalyze the synthesis of diacylglycerols and retinyl esters; however this activity is unclear in vivo (By similarity).By similarity

Catalytic activityi

Acyl-CoA + a long-chain alcohol = CoA + a long-chain ester.
Acyl-CoA + retinol = CoA + retinyl ester.

GO - Molecular functioni

GO - Biological processi

  • retinol metabolic process Source: MGI
  • wax biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.75. 3474.
ReactomeiR-MMU-1482883. Acyl chain remodeling of DAG and TAG.
R-MMU-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
R-MMU-8848584. Wax biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-CoA wax alcohol acyltransferase 2 (EC:2.3.1.75)
Alternative name(s):
Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
Short name:
ARAT
Short name:
Retinol O-fatty-acyltransferase
Diacylglycerol O-acyltransferase 2-like protein 4
Long-chain-alcohol O-fatty-acyltransferase 2
Wax synthase
Short name:
mWS
Gene namesi
Name:Awat2
Synonyms:Dgat2l4, Ws
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:3045345. Awat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3521HelicalSequence analysisAdd
BLAST
Transmembranei38 – 5821HelicalSequence analysisAdd
BLAST
Transmembranei130 – 15021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 333333Acyl-CoA wax alcohol acyltransferase 2PRO_0000249053Add
BLAST

Proteomic databases

PaxDbiQ6E1M8.
PRIDEiQ6E1M8.

PTM databases

PhosphoSiteiQ6E1M8.

Expressioni

Gene expression databases

BgeeiQ6E1M8.
CleanExiMM_DGAT2L4.
ExpressionAtlasiQ6E1M8. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033567.

Structurei

3D structure databases

ProteinModelPortaliQ6E1M8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ6E1M8.
KOiK11156.
OMAiYPCAFYG.
OrthoDBiEOG7KH9KB.
PhylomeDBiQ6E1M8.
TreeFamiTF314707.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6E1M8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFWPTKKDLK TAMEVFALFQ WALSALVIVT TVIIVNLYLV VFTSYWPVTV
60 70 80 90 100
LMLTWLAFDW KTPERGGRRF TCVRKWRLWK HYSDYFPLKM VKTKDISPDR
110 120 130 140 150
NYILVCHPHG LMAHSCFGHF ATDTTGFSKT FPGITPYMLT LGAFFWVPFL
160 170 180 190 200
RDYVMSTGSC SVSRSSMDFL LTQKGTGNML VVVVGGLAEC RYSTPGSTTL
210 220 230 240 250
FLKKRQGFVR TALKHGVSLI PAYAFGETDL YDQHIFTPGG FVNRFQKWFQ
260 270 280 290 300
KMVHIYPCAF YGRGLTKNSW GLLPYSQPVT TVVGEPLPLP KIENPSEEIV
310 320 330
AKYHTLYIDA LRKLFDQHKT KFGISETQEL VIV
Length:333
Mass (Da):38,145
Last modified:August 16, 2004 - v1
Checksum:iA2A2036B28F424A4
GO
Isoform 2 (identifier: Q6E1M8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Note: No experimental confirmation available.
Show »
Length:282
Mass (Da):32,255
Checksum:i7D459F383E7EE1CF
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5151Missing in isoform 2. 1 PublicationVSP_020357Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY611031 mRNA. Translation: AAT68765.1.
AY611032 mRNA. Translation: AAT68766.1.
AK034920 mRNA. Translation: BAC28882.1.
CCDSiCCDS30300.1. [Q6E1M8-1]
CCDS72412.1. [Q6E1M8-2]
RefSeqiNP_001277324.1. NM_001290395.1. [Q6E1M8-2]
NP_808414.2. NM_177746.4. [Q6E1M8-1]
XP_011245890.1. XM_011247588.1. [Q6E1M8-2]
XP_011245891.1. XM_011247589.1. [Q6E1M8-2]
XP_011245892.1. XM_011247590.1. [Q6E1M8-2]
XP_011245893.1. XM_011247591.1. [Q6E1M8-2]
XP_011245894.1. XM_011247592.1. [Q6E1M8-2]
UniGeneiMm.118171.

Genome annotation databases

EnsembliENSMUST00000033567; ENSMUSP00000033567; ENSMUSG00000031220. [Q6E1M8-1]
ENSMUST00000147103; ENSMUSP00000128516; ENSMUSG00000031220. [Q6E1M8-2]
GeneIDi245532.
KEGGimmu:245532.
UCSCiuc009tvx.2. mouse. [Q6E1M8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY611031 mRNA. Translation: AAT68765.1.
AY611032 mRNA. Translation: AAT68766.1.
AK034920 mRNA. Translation: BAC28882.1.
CCDSiCCDS30300.1. [Q6E1M8-1]
CCDS72412.1. [Q6E1M8-2]
RefSeqiNP_001277324.1. NM_001290395.1. [Q6E1M8-2]
NP_808414.2. NM_177746.4. [Q6E1M8-1]
XP_011245890.1. XM_011247588.1. [Q6E1M8-2]
XP_011245891.1. XM_011247589.1. [Q6E1M8-2]
XP_011245892.1. XM_011247590.1. [Q6E1M8-2]
XP_011245893.1. XM_011247591.1. [Q6E1M8-2]
XP_011245894.1. XM_011247592.1. [Q6E1M8-2]
UniGeneiMm.118171.

3D structure databases

ProteinModelPortaliQ6E1M8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033567.

PTM databases

PhosphoSiteiQ6E1M8.

Proteomic databases

PaxDbiQ6E1M8.
PRIDEiQ6E1M8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033567; ENSMUSP00000033567; ENSMUSG00000031220. [Q6E1M8-1]
ENSMUST00000147103; ENSMUSP00000128516; ENSMUSG00000031220. [Q6E1M8-2]
GeneIDi245532.
KEGGimmu:245532.
UCSCiuc009tvx.2. mouse. [Q6E1M8-1]

Organism-specific databases

CTDi158835.
MGIiMGI:3045345. Awat2.

Phylogenomic databases

eggNOGiKOG0831. Eukaryota.
ENOG410XTG3. LUCA.
GeneTreeiENSGT00750000117391.
HOGENOMiHOG000179738.
HOVERGENiHBG065791.
InParanoidiQ6E1M8.
KOiK11156.
OMAiYPCAFYG.
OrthoDBiEOG7KH9KB.
PhylomeDBiQ6E1M8.
TreeFamiTF314707.

Enzyme and pathway databases

BRENDAi2.3.1.75. 3474.
ReactomeiR-MMU-1482883. Acyl chain remodeling of DAG and TAG.
R-MMU-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
R-MMU-8848584. Wax biosynthesis.

Miscellaneous databases

PROiQ6E1M8.
SOURCEiSearch...

Gene expression databases

BgeeiQ6E1M8.
CleanExiMM_DGAT2L4.
ExpressionAtlasiQ6E1M8. baseline and differential.

Family and domain databases

InterProiIPR007130. DAGAT.
[Graphical view]
PfamiPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian wax biosynthesis. II. Expression cloning of wax synthase cDNAs encoding a member of the acyltransferase enzyme family."
    Cheng J.B., Russell D.W.
    J. Biol. Chem. 279:37798-37807(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Embryo.

Entry informationi

Entry nameiAWAT2_MOUSE
AccessioniPrimary (citable) accession number: Q6E1M8
Secondary accession number(s): Q8BM49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: August 16, 2004
Last modified: July 6, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.