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Protein

Katanin p60 ATPase-containing subunit A1

Gene

Katna1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.UniRule annotation2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Enzyme regulationi

ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with KATNB1.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2568ATPUniRule annotation

GO - Molecular functioni

  • ATPase activity Source: RGD
  • ATP binding Source: RGD
  • microtubule-severing ATPase activity Source: UniProtKB-EC

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • microtubule severing Source: RGD
  • mitotic nuclear division Source: RGD
  • negative regulation of neuron projection development Source: RGD
  • protein localization Source: RGD
  • protein ubiquitination Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.3. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Katanin p60 ATPase-containing subunit A1UniRule annotation (EC:3.6.4.3UniRule annotation)
Short name:
Katanin p60 subunit A1UniRule annotation
Alternative name(s):
p60 kataninUniRule annotation
Gene namesi
Name:Katna1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1303062. Katna1.

Subcellular locationi

  • Cytoplasm
  • Midbody UniRule annotation
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome
  • Cytoplasmcytoskeletonspindle pole UniRule annotation

  • Note: Predominantly cytoplasmic. Localized diffusely in the cytoplasm during the interphase. During metaphase is localized throughout the cell and more widely dispersed than the microtubules. In anaphase and telophase is localized at the midbody region. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.UniRule annotation

GO - Cellular componenti

  • axon Source: RGD
  • centrosome Source: UniProtKB-HAMAP
  • cytoplasm Source: UniProtKB
  • growth cone Source: RGD
  • microtubule Source: UniProtKB-KW
  • midbody Source: UniProtKB
  • neuronal cell body Source: RGD
  • nucleus Source: RGD
  • spindle Source: UniProtKB
  • spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi255 – 2551K → A: Abolishes ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Katanin p60 ATPase-containing subunit A1PRO_0000084596Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421Phosphoserine; by DYRK2UniRule annotationBy similarity

Post-translational modificationi

Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.UniRule annotation
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.UniRule annotation

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6E0V2.
PRIDEiQ6E0V2.

Interactioni

Subunit structurei

Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit. Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42.UniRule annotation

Protein-protein interaction databases

BioGridi253846. 16 interactions.
STRINGi10116.ENSRNOP00000020417.

Structurei

3D structure databases

ProteinModelPortaliQ6E0V2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 185185Interaction with microtubulesBy similarityAdd
BLAST
Regioni1 – 7575Interaction with dynein and NDEL1By similarityAdd
BLAST
Regioni1 – 2929Interaction with KATNB1By similarityAdd
BLAST

Domaini

The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0738. Eukaryota.
ENOG410XPN7. LUCA.
HOGENOMiHOG000225142.
HOVERGENiHBG057074.
InParanoidiQ6E0V2.
KOiK07767.
PhylomeDBiQ6E0V2.
TreeFamiTF323170.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_03023. Katanin_p60_A1.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR028596. KATNA1.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6E0V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLMITENV KLAREYALLG NYDSAMVYYQ GVLDQINKYL YSVKDTHLHQ
60 70 80 90 100
KWQQVWQEIN VEAKHVKEIM KTLESFKLDS TSLKAAQHEL PSSEGEVWSL
110 120 130 140 150
PVPVERRPLP GPRKRQSTQH SDPKPHSNRP GAVVRAHRPS AQSLHSDRGK
160 170 180 190 200
AVRSREKKEQ SKGREEKNKL PAAVTEPEAN KFDSTGYDKD LVEALERDII
210 220 230 240 250
SQNPNVRWYD IADLVEAKKL LQEAVVLPMW MPEFFKGIRR PWKGVLMVGP
260 270 280 290 300
PGTGKTLLAK AVATECKTTF FNVSSSTLTS KYRGESEKLV RLLFEMARFY
310 320 330 340 350
SPATIFIDEI DSICSRRGTS EEHEASRRVK AELLVQMDGV GGASENDDPS
360 370 380 390 400
KMVMVLAATN FPWDIDEALR RRLEKRIYIP LPSAKGREEL LRISLRELEL
410 420 430 440 450
ADDVNLASIA ENMEGYSGAD ITNVCRDASL MAMRRRIEGL TPEEIRNLSR
460 470 480 490
EEMHMPTTME DFEMALKKVS KSVSAADIER YEKWIVEFGS C
Length:491
Mass (Da):55,844
Last modified:August 16, 2004 - v1
Checksum:iAE85B1B307A37AA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY621629 mRNA. Translation: AAT44333.1.
BC097929 mRNA. Translation: AAH97929.1.
RefSeqiNP_001004217.2. NM_001004217.4.
UniGeneiRn.47464.

Genome annotation databases

GeneIDi292464.
KEGGirno:292464.
UCSCiRGD:1303062. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY621629 mRNA. Translation: AAT44333.1.
BC097929 mRNA. Translation: AAH97929.1.
RefSeqiNP_001004217.2. NM_001004217.4.
UniGeneiRn.47464.

3D structure databases

ProteinModelPortaliQ6E0V2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi253846. 16 interactions.
STRINGi10116.ENSRNOP00000020417.

Proteomic databases

PaxDbiQ6E0V2.
PRIDEiQ6E0V2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi292464.
KEGGirno:292464.
UCSCiRGD:1303062. rat.

Organism-specific databases

CTDi11104.
RGDi1303062. Katna1.

Phylogenomic databases

eggNOGiKOG0738. Eukaryota.
ENOG410XPN7. LUCA.
HOGENOMiHOG000225142.
HOVERGENiHBG057074.
InParanoidiQ6E0V2.
KOiK07767.
PhylomeDBiQ6E0V2.
TreeFamiTF323170.

Enzyme and pathway databases

BRENDAi3.6.4.3. 5301.

Miscellaneous databases

PROiQ6E0V2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_03023. Katanin_p60_A1.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR028596. KATNA1.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Axonal growth is sensitive to the levels of katanin, a protein that severs microtubules."
    Karabay A., Yu W., Solowska J.M., Baird D.H., Baas P.W.
    J. Neurosci. 24:5778-5788(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-255.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  3. "An essential role for katanin in severing microtubules in the neuron."
    Ahmad F.J., Yu W., McNally F.J., Baas P.W.
    J. Cell Biol. 145:305-315(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKTNA1_RAT
AccessioniPrimary (citable) accession number: Q6E0V2
Secondary accession number(s): Q4V7G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: August 16, 2004
Last modified: June 8, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.