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Protein

Katanin p60 ATPase-containing subunit A1

Gene

Katna1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.UniRule annotation2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Enzyme regulationi

ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with KATNB1.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi249 – 256ATPUniRule annotation8

GO - Molecular functioni

  • ATPase activity Source: RGD
  • ATP binding Source: RGD
  • microtubule binding Source: InterPro
  • microtubule-severing ATPase activity Source: UniProtKB-EC

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • microtubule severing Source: RGD
  • mitotic cell cycle Source: RGD
  • negative regulation of neuron projection development Source: RGD
  • protein localization Source: RGD

Keywordsi

Molecular functionHydrolase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.3. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Katanin p60 ATPase-containing subunit A1UniRule annotation (EC:3.6.4.3UniRule annotation)
Short name:
Katanin p60 subunit A1UniRule annotation
Alternative name(s):
p60 kataninUniRule annotation
Gene namesi
Name:Katna1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1303062. Katna1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi255K → A: Abolishes ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000845961 – 491Katanin p60 ATPase-containing subunit A1Add BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42Phosphoserine; by DYRK2UniRule annotationBy similarity1

Post-translational modificationi

Phosphorylation by DYRK2 triggers ubiquitination and subsequent degradation.UniRule annotation
Ubiquitinated by the BCR(KLHL42) E3 ubiquitin ligase complex, leading to its proteasomal degradation. Ubiquitinated by the EDVP E3 ligase complex and subsequently targeted for proteasomal degradation.UniRule annotation

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6E0V2.
PRIDEiQ6E0V2.

Interactioni

Subunit structurei

Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit (By similarity). Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM (By similarity). Interacts with dynein and NDEL1. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and DCAF1 proteins (EDVP complex). Interacts with KLHL42 (via the kelch domains). Interacts with CUL3; the interaction is enhanced by KLHL42 (By similarity). Interacts with KATNB1 and KATNBL1 (By similarity).UniRule annotationBy similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi253846. 16 interactors.
STRINGi10116.ENSRNOP00000020417.

Structurei

3D structure databases

ProteinModelPortaliQ6E0V2.
SMRiQ6E0V2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 185Interaction with microtubulesBy similarityAdd BLAST185
Regioni1 – 75Interaction with dynein and NDEL1By similarityAdd BLAST75
Regioni1 – 29Interaction with KATNB1By similarityAdd BLAST29

Domaini

The N-terminus is sufficient for interaction with microtubules, although high affinity binding to microtubules also requires an intact C-terminal domain and ATP, which promotes oligomerization.UniRule annotation

Sequence similaritiesi

Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG0738. Eukaryota.
ENOG410XPN7. LUCA.
HOGENOMiHOG000225142.
HOVERGENiHBG057074.
InParanoidiQ6E0V2.
KOiK07767.
PhylomeDBiQ6E0V2.
TreeFamiTF323170.

Family and domain databases

HAMAPiMF_03023. Katanin_p60_A1. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR028596. KATNA1.
IPR027417. P-loop_NTPase.
IPR015415. Vps4_C.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
PF09336. Vps4_C. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6E0V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLMITENV KLAREYALLG NYDSAMVYYQ GVLDQINKYL YSVKDTHLHQ
60 70 80 90 100
KWQQVWQEIN VEAKHVKEIM KTLESFKLDS TSLKAAQHEL PSSEGEVWSL
110 120 130 140 150
PVPVERRPLP GPRKRQSTQH SDPKPHSNRP GAVVRAHRPS AQSLHSDRGK
160 170 180 190 200
AVRSREKKEQ SKGREEKNKL PAAVTEPEAN KFDSTGYDKD LVEALERDII
210 220 230 240 250
SQNPNVRWYD IADLVEAKKL LQEAVVLPMW MPEFFKGIRR PWKGVLMVGP
260 270 280 290 300
PGTGKTLLAK AVATECKTTF FNVSSSTLTS KYRGESEKLV RLLFEMARFY
310 320 330 340 350
SPATIFIDEI DSICSRRGTS EEHEASRRVK AELLVQMDGV GGASENDDPS
360 370 380 390 400
KMVMVLAATN FPWDIDEALR RRLEKRIYIP LPSAKGREEL LRISLRELEL
410 420 430 440 450
ADDVNLASIA ENMEGYSGAD ITNVCRDASL MAMRRRIEGL TPEEIRNLSR
460 470 480 490
EEMHMPTTME DFEMALKKVS KSVSAADIER YEKWIVEFGS C
Length:491
Mass (Da):55,844
Last modified:August 16, 2004 - v1
Checksum:iAE85B1B307A37AA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY621629 mRNA. Translation: AAT44333.1.
BC097929 mRNA. Translation: AAH97929.1.
RefSeqiNP_001004217.2. NM_001004217.4.
UniGeneiRn.47464.

Genome annotation databases

GeneIDi292464.
KEGGirno:292464.
UCSCiRGD:1303062. rat.

Similar proteinsi

Entry informationi

Entry nameiKTNA1_RAT
AccessioniPrimary (citable) accession number: Q6E0V2
Secondary accession number(s): Q4V7G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: August 16, 2004
Last modified: September 27, 2017
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families