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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Gene

Enpp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.By similarity

Catalytic activityi

Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.
A dinucleotide + H2O = 2 mononucleotides.

Cofactori

a divalent metal cationCuratedNote: Binds 2 divalent metal cations per subunit.Curated

Enzyme regulationi

At low concentrations of ATP, a phosphorylated active site intermediate is formed which inhibits further ATP hydrolysis.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi167 – 1671Divalent metal cation 2Curated
Active sitei205 – 2051AMP-threonine intermediateBy similarity
Metal bindingi325 – 3251Divalent metal cation 1Curated
Metal bindingi329 – 3291Divalent metal cation 1Curated
Metal bindingi372 – 3721Divalent metal cation 2Curated
Metal bindingi373 – 3731Divalent metal cation 2Curated
Metal bindingi482 – 4821Divalent metal cation 1Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
Short name:
E-NPP 3
Alternative name(s):
Phosphodiesterase I beta
Short name:
PD-Ibeta
Phosphodiesterase I/nucleotide pyrophosphatase 3
CD_antigen: CD203c
Including the following 2 domains:
Alkaline phosphodiesterase I (EC:3.1.4.1)
Nucleotide pyrophosphatase (EC:3.6.1.9)
Short name:
NPPase
Gene namesi
Name:Enpp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2143702. Enpp3.

Subcellular locationi

  • Membrane Curated; Single-pass type II membrane protein Curated
  • Secreted By similarity

  • Note: Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3019Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini31 – 874844ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Ectonucleotide pyrophosphatase/phosphodiesterase family member 3PRO_0000281652Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 71PROSITE-ProRule annotation
Disulfide bondi54 ↔ 58PROSITE-ProRule annotation
Disulfide bondi58 ↔ 89PROSITE-ProRule annotation
Disulfide bondi69 ↔ 82PROSITE-ProRule annotation
Disulfide bondi69 ↔ 71PROSITE-ProRule annotation
Disulfide bondi75 ↔ 81PROSITE-ProRule annotation
Disulfide bondi82 ↔ 89PROSITE-ProRule annotation
Disulfide bondi98 ↔ 115AlternatePROSITE-ProRule annotation
Disulfide bondi98 ↔ 103AlternatePROSITE-ProRule annotation
Disulfide bondi103 ↔ 133AlternatePROSITE-ProRule annotation
Disulfide bondi113 ↔ 126AlternatePROSITE-ProRule annotation
Disulfide bondi113 ↔ 115AlternatePROSITE-ProRule annotation
Disulfide bondi119 ↔ 125PROSITE-ProRule annotation
Disulfide bondi126 ↔ 133AlternatePROSITE-ProRule annotation
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence analysis
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence analysis
Glycosylationi532 – 5321N-linked (GlcNAc...)Sequence analysis
Glycosylationi594 – 5941N-linked (GlcNAc...)1 Publication
Glycosylationi687 – 6871N-linked (GlcNAc...)Sequence analysis
Glycosylationi701 – 7011N-linked (GlcNAc...)Sequence analysis
Glycosylationi820 – 8201N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal.By similarity
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ6DYE8.
PaxDbiQ6DYE8.
PRIDEiQ6DYE8.

PTM databases

iPTMnetiQ6DYE8.
PhosphoSiteiQ6DYE8.

Expressioni

Gene expression databases

BgeeiQ6DYE8.
GenevisibleiQ6DYE8. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020169.

Structurei

3D structure databases

ProteinModelPortaliQ6DYE8.
SMRiQ6DYE8. Positions 53-870.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 9343SMB 1PROSITE-ProRule annotationAdd
BLAST
Domaini94 – 13845SMB 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 509370PhosphodiesteraseAdd
BLAST
Regioni605 – 874270NucleaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi78 – 803Cell attachment siteSequence analysis

Sequence similaritiesi

Contains 2 SMB (somatomedin-B) domains.Curated

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000037439.
HOVERGENiHBG051484.
InParanoidiQ6DYE8.
KOiK01513.
OMAiRTSDSQY.
OrthoDBiEOG7XM2X4.
TreeFamiTF330032.

Family and domain databases

Gene3Di3.40.570.10. 1 hit.
3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR024873. E-NPP.
IPR029885. ENPP3.
IPR020821. Extracellular_endonuc_su_A.
IPR002591. Phosphodiest/P_Trfase.
IPR001212. Somatomedin_B_dom.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF64. PTHR10151:SF64. 1 hit.
PfamiPF01223. Endonuclease_NS. 1 hit.
PF01663. Phosphodiest. 1 hit.
PF01033. Somatomedin_B. 2 hits.
[Graphical view]
SMARTiSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
SM00201. SO. 2 hits.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
SSF90188. SSF90188. 2 hits.
PROSITEiPS00524. SMB_1. 2 hits.
PS50958. SMB_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DYE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSRLALATE EPIKKDSLKK YKILCVVLLA LLVIVSLGLG LGLGLRKPEE
60 70 80 90 100
QGSCRKKCFD SSHRGLEGCR CDSGCTGRGD CCWDFEDTCV KSTQIWTCNL
110 120 130 140 150
FRCGENRLET ALCSCADDCL QRKDCCADYK TVCQGESPWV TEACASSQEP
160 170 180 190 200
QCPPGFDLPP VILFSMDGFR AEYLQTWSTL LPNINKLKTC GIHSKYMRAM
210 220 230 240 250
YPTKTFPNHY TIVTGLYPES HGIIDNNMYD VHLNKNFSLS SVEKSNPAWW
260 270 280 290 300
SGQPIWLTAM YQGLKAACYY WPGSDVAVNG SFPTIYRNYS NSVPYERRIT
310 320 330 340 350
TLLQWLDLPK ADRPSFYTIY VEEPDSAGHS SGPVSAGVIK ALQSVDNAFG
360 370 380 390 400
MLMEGLKQRN LHNCVNIIVL ADHGMDQTSC DRVEYMTDYF PKINFYMYQG
410 420 430 440 450
PAPRIRTRNI PQDFFTFNSE EIVRNLSCRK PDQHFKPYLT PDLPKRLHYA
460 470 480 490 500
KNVRIDKAHL MVDRQWLAFR SKGSSNCGGG THGYNNEFKS MEAIFLAHGP
510 520 530 540 550
SFIEKTVIEP FENIEVYNLL CDLLHIEPAP NNGTHGSLNH LLKTPFYKPS
560 570 580 590 600
HAGELSTPAD CGFTTPLPTD PLDCSCPALQ NTPGLEEQAN QRLNLSEGEV
610 620 630 640 650
AATVKANLPF GRPRVMQKNG DHCLLYHRDY ISGYGKAMKM PMWSSYTVLK
660 670 680 690 700
PGDTSSLPPT VPDCLRADVR VAPSESQKCS FYLADKNITH GFLYPAIKGT
710 720 730 740 750
NESRYDALIT SNLVPMYKEF KKMWDYFHEV LLIKYAIERN GLNVVSGPIF
760 770 780 790 800
DYNYDGHFDA PDEITQYVAG TDVPIPTHYF VVLTSCKDQT HTPDSCPGWL
810 820 830 840 850
DVLPFIVPHR PTNIESCSEN KTEDLWVEER FQAHAARVRD VELLTGLDFY
860 870
QEKAQPVSQI LQLKTYLPTF ETII
Length:874
Mass (Da):98,662
Last modified:July 27, 2011 - v2
Checksum:i6CFF970ECC830D08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti212 – 2121I → S in AAT64421 (Ref. 1) Curated
Sequence conflicti560 – 5601D → V in AAT64421 (Ref. 1) Curated
Sequence conflicti835 – 8351A → V in AAT64421 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY630402 mRNA. Translation: AAT64421.1.
AC158616 Genomic DNA. No translation available.
CCDSiCCDS23752.1.
RefSeqiNP_598766.2. NM_134005.2.
UniGeneiMm.338425.

Genome annotation databases

EnsembliENSMUST00000020169; ENSMUSP00000020169; ENSMUSG00000019989.
GeneIDi209558.
KEGGimmu:209558.
UCSCiuc007era.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY630402 mRNA. Translation: AAT64421.1.
AC158616 Genomic DNA. No translation available.
CCDSiCCDS23752.1.
RefSeqiNP_598766.2. NM_134005.2.
UniGeneiMm.338425.

3D structure databases

ProteinModelPortaliQ6DYE8.
SMRiQ6DYE8. Positions 53-870.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020169.

PTM databases

iPTMnetiQ6DYE8.
PhosphoSiteiQ6DYE8.

Proteomic databases

MaxQBiQ6DYE8.
PaxDbiQ6DYE8.
PRIDEiQ6DYE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020169; ENSMUSP00000020169; ENSMUSG00000019989.
GeneIDi209558.
KEGGimmu:209558.
UCSCiuc007era.1. mouse.

Organism-specific databases

CTDi5169.
MGIiMGI:2143702. Enpp3.

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000037439.
HOVERGENiHBG051484.
InParanoidiQ6DYE8.
KOiK01513.
OMAiRTSDSQY.
OrthoDBiEOG7XM2X4.
TreeFamiTF330032.

Miscellaneous databases

ChiTaRSiEnpp3. mouse.
NextBioi372722.
PROiQ6DYE8.
SOURCEiSearch...

Gene expression databases

BgeeiQ6DYE8.
GenevisibleiQ6DYE8. MM.

Family and domain databases

Gene3Di3.40.570.10. 1 hit.
3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR024873. E-NPP.
IPR029885. ENPP3.
IPR020821. Extracellular_endonuc_su_A.
IPR002591. Phosphodiest/P_Trfase.
IPR001212. Somatomedin_B_dom.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF64. PTHR10151:SF64. 1 hit.
PfamiPF01223. Endonuclease_NS. 1 hit.
PF01663. Phosphodiest. 1 hit.
PF01033. Somatomedin_B. 2 hits.
[Graphical view]
SMARTiSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
SM00201. SO. 2 hits.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
SSF90188. SSF90188. 2 hits.
PROSITEiPS00524. SMB_1. 2 hits.
PS50958. SMB_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of mouse ENPP3 (gp130RB13-6) as a lysophospholipase D."
    Lynch K.R., Lee S.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-594.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Kidney, Liver and Lung.

Entry informationi

Entry nameiENPP3_MOUSE
AccessioniPrimary (citable) accession number: Q6DYE8
Secondary accession number(s): E9QMU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 27, 2011
Last modified: February 17, 2016
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.