Reviewed, UniProtKB/Swiss-Prot Q6DYE7 (RENI_CANFA)

Names and origin

Protein names

Recommended name:
    Renin
    EC=3.4.23.15
Alternative name(s):
    Angiotensinogenase

Gene names

Name:

REN

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney By similarity.
Catalytic activity	Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I.
Enzyme regulation	Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing By similarity.
Subunit structure	Interacts with ATP6AP2 By similarity.
Subcellular location	SecretedBy similarity. MembraneBy similarity. Note= Associated to membranes via binding to ATP6AP2 By similarity.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Membrane Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Cleavage on pair of basic residues Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 65	43	Activation peptide By similarity		PRO_0000026079
	Chain	66 – 403	338	Renin		PRO_0000026080
Sites
	Active site	103	1	By similarity
	Active site	288	1	By similarity
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Disulfide bond	116 ↔ 123		By similarity
	Disulfide bond	279 ↔ 283		By similarity
	Disulfide bond	322 ↔ 359		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DYE7-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: B63101337155AE44	FASTA	403	44,099
	10 20 30 40 50 60 MARCRMPRWG LLLVLWGSCT FGLPADTGAF RRIFLKKMPS IRESLKERGV DVAGLGAEWN 70 80 90 100 110 120 QFTKRLSSGN STSPVVLTNY LDTQYYGEIG IGTPPQTFKV VFDTGSANLW VPSTRCSPLY 130 140 150 160 170 180 TACEIHCLYD SSESSSYMEN GTTFTIRYGS GKVKGFLSQD MVTVGGITVT QTFGEVTELP 190 200 210 220 230 240 LIPFMLAKFD GVLGMGFPAQ AVGGVTPVFD HILSQGVLKE EVFSVYYSRN SHLLGGEVVL 250 260 270 280 290 300 GGSDPQYYQG NFHYVSISKT GSWQIKMKGV SVRSATLVCE EGCMVVVDTG ASYISGPTSS 310 320 330 340 350 360 LRLLMDTLGA QELSTNEYVV NCNQVPTLPD ISFHLGGRAY TLTSKDYVLQ DPYGNEDLCT 370 380 390 400 LALHGLDVPP PTGPVWVLGA SFIRKFYTEF DRHNNRIGFA LAR

References

[1]	"The cloning and characterization of canine renin." Zecher M., Wu Z., Scott B., McGeehan G., Harrison R. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY630442 mRNA. Translation: AAT68959.1.
RefSeq	NP_001003194.1.
UniGene	Cfa.3701
3D structure databases
SMR	Q6DYE7. Positions 69-403.
ModBase	Search...
Protein family/group databases
MEROPS	A01.007.
Genome annotation databases
Ensembl	ENSCAFG00000009623. Canis familiaris. [Contig view]
GeneID	403838.
KEGG	cfa:403838.
Phylogenomic databases
HOVERGEN	Q6DYE7.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RENI_CANFA

Accession

Primary (citable) accession number: Q6DYE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)