Reviewed, UniProtKB/Swiss-Prot Q6DV14 (PRDX1_GECJA)

Names and origin

Protein names

Recommended name:
    Peroxiredoxin-1
    EC=1.11.1.15

Gene names

Name:	PRDX1
ORF Names:	GekBS014P

Organism

Gecko japonicus (Japanese gecko)

Taxonomic identifier

146911 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Gekkota › Gekkonidae › Gekkoninae › Gekko

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.
Subunit structure	Homodimer; disulfide-linked, upon oxidation By similarity.
Subcellular location	CytoplasmBy similarity.
Miscellaneous	The active site is the redox-active Cys-52 oxidized to Cys-SOH. Cys-SOH rapidly reacts with Cys-173-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin By similarity. Inactivated upon oxidative stress by overoxidation of Cys-52 to Cys-SO(2)H and Cys-SO(3)H. Cys-SO(2)H is retroreduced to Cys-SOH after removal of H(2)O(2), while Cys-SO(3)H may be irreversibly oxidized By similarity.
Sequence similarities	Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Peroxiredoxin-1		PRO_0000256854
Regions
	Domain	6 – 165	160	Thioredoxin
Sites
	Active site	52	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Modified residue	90	1	Phosphothreonine By similarity
	Modified residue	194	1	Phosphotyrosine By similarity
	Disulfide bond	52		Interchain (with C-173); in linked form By similarity
	Disulfide bond	173		Interchain (with C-52); in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DV14-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 6AB33FB90907C318	FASTA	199	22,348
	10 20 30 40 50 60 MSSGNAYIGK LAPDFQATAV MPDGQFKEIK LSDYKGKYVV LFFYPLDFTF VCPTEIIAFS 70 80 90 100 110 120 DRSEEFRKIN CEVIGASVDS HFCHLAWINT PKKQGGLGSM HIPLVSDTKR VIAKDYGILK 130 140 150 160 170 180 EDEGISYRGL FIIDDKGTLR QITINDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWQPG 190 SDTIKPDVQK SKEYFSKHK

References

[1]

"Analysis of expressed sequence tags and cloning of full length cDNA from brain and spinal cord cDNA library in Gecko." Ding F., Liu Y., Gu X., Tan X., Shen A., Zhang H., Liu M., Jiang M., Yang H. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY626813 mRNA. Translation: AAT85554.1. AY641835 mRNA. Translation: AAT68217.1.
3D structure databases
SMR	Q6DV14. Positions 3-198.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q6DV14.
Family and domain databases
InterPro	IPR000866. AhpC-TSA. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00578. AhpC-TSA. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRDX1_GECJA

Accession

Primary (citable) accession number: Q6DV14

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)