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Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4

Gene

Pfkfb4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.By similarity

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Enzyme regulationi

The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79Fructose 6-phosphateBy similarity1
Binding sitei103Fructose 6-phosphateBy similarity1
Active sitei129Sequence analysis1
Binding sitei131Fructose 6-phosphateBy similarity1
Binding sitei137Fructose 6-phosphateBy similarity1
Active sitei159Sequence analysis1
Binding sitei173Fructose 6-phosphateBy similarity1
Binding sitei194Fructose 6-phosphateBy similarity1
Binding sitei198Fructose 6-phosphateBy similarity1
Binding sitei256Fructose 2,6-bisphosphateBy similarity1
Active sitei257Tele-phosphohistidine intermediateBy similarity1
Binding sitei263Fructose 2,6-bisphosphateBy similarity1
Binding sitei269Fructose 2,6-bisphosphate; via amide nitrogenBy similarity1
Binding sitei306Fructose 2,6-bisphosphateBy similarity1
Active sitei326Proton donor/acceptorBy similarity1
Binding sitei337Fructose 2,6-bisphosphateBy similarity1
Binding sitei351Fructose 2,6-bisphosphateBy similarity1
Binding sitei355Fructose 2,6-bisphosphateBy similarity1
Binding sitei366Fructose 2,6-bisphosphateBy similarity1
Sitei391Transition state stabilizerBy similarity1
Binding sitei392Fructose 2,6-bisphosphateBy similarity1
Binding sitei396Fructose 2,6-bisphosphateBy similarity1
Binding sitei428ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPBy similarity9
Nucleotide bindingi168 – 173ATPBy similarity6
Nucleotide bindingi348 – 351ATPBy similarity4
Nucleotide bindingi392 – 396ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-70171. Glycolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
Short name:
6PF-2-K/Fru-2,6-P2ase 4
Short name:
PFK/FBPase 4
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase testis-type isozyme
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:Pfkfb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2687284. Pfkfb4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002681871 – 4696-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4Add BLAST469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29Phosphoserine; by PKCSequence analysis1
Modified residuei444Phosphothreonine; by PKCSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6DTY7.
PeptideAtlasiQ6DTY7.
PRIDEiQ6DTY7.

PTM databases

iPTMnetiQ6DTY7.
PhosphoSitePlusiQ6DTY7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025648.
ExpressionAtlasiQ6DTY7. baseline and differential.
GenevisibleiQ6DTY7. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057197.

Structurei

3D structure databases

ProteinModelPortaliQ6DTY7.
SMRiQ6DTY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 2496-phosphofructo-2-kinaseAdd BLAST249
Regioni250 – 469Fructose-2,6-bisphosphataseAdd BLAST220

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiKOG0234. Eukaryota.
COG0406. LUCA.
GeneTreeiENSGT00390000018751.
HOGENOMiHOG000181112.
HOVERGENiHBG005628.
InParanoidiQ6DTY7.
KOiK19030.
OMAiDCSNDEA.
OrthoDBiEOG091G0A43.
PhylomeDBiQ6DTY7.
TreeFamiTF313541.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6DTY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPRELTQN PLKKIWMPYS NGRPALHASQ RGVCMTNCPT LIVMVGLPAR
60 70 80 90 100
GKTYISKKLT RYLNWIGVPT REFNVGQYRR DIVKTYKSFE FFLPDNEEGL
110 120 130 140 150
KIRKQCALAA LSDVRKFLSE EGGHVAVFDA TNTTRERRAM IFNFGEQNGY
160 170 180 190 200
KTFFVESICV DPEVVAANIV QVKLGSPDYV NRDSDEATED FMRRIECYEN
210 220 230 240 250
SYESLDEDLD RDLSYIKIMD VGQSYVVNRV ADHIQSRIVY YLMNIHVTPR
260 270 280 290 300
SIYLCRHGES ELNLKGRIGG DPGLSPRGRE FSKHLAQFIS DQNIKDLKVW
310 320 330 340 350
TSQMKRTIQT AEALSVPYEQ WKVLNEIDAG VCEEMTYEEI QDHYPLEFAL
360 370 380 390 400
RDQDKYRYRY PKGESYEDLV QRLEPVIMEL ERQENVLVIC HQAVMRCLLA
410 420 430 440 450
YFLDKAAEEL PYLKCPLHTV LKLTPVAYGC KVESIFLNVA AVNTHRDRPQ
460
NVDISRPSEE ALVTVPAHQ
Length:469
Mass (Da):54,067
Last modified:January 23, 2007 - v4
Checksum:i53777000F163DDE7
GO
Isoform 2 (identifier: Q6DTY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-218: Missing.

Show »
Length:251
Mass (Da):29,036
Checksum:i2DE38D2AF5D41D05
GO
Isoform 3 (identifier: Q6DTY7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     358-469: YRYPKGESYE...EALVTVPAHQ → PMKTWCSGWS...PTSNAPCTQS

Show »
Length:413
Mass (Da):47,601
Checksum:i168599E96293CB8E
GO
Isoform 4 (identifier: Q6DTY7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-221: DLSYIKIMDV → SSGQSRRLFS
     222-469: Missing.

Show »
Length:221
Mass (Da):25,322
Checksum:i021463A4216FB0DC
GO
Isoform 5 (identifier: Q6DTY7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     33-48: Missing.

Show »
Length:453
Mass (Da):52,394
Checksum:i45892FD5B58EB548
GO
Isoform 6 (identifier: Q6DTY7-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Show »
Length:435
Mass (Da):50,246
Checksum:iA87B2F6B22059987
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0219591 – 218Missing in isoform 2. 1 PublicationAdd BLAST218
Alternative sequenceiVSP_0219601 – 34Missing in isoform 6. 1 PublicationAdd BLAST34
Alternative sequenceiVSP_02196133 – 48Missing in isoform 5. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_021962212 – 221DLSYIKIMDV → SSGQSRRLFS in isoform 4. 1 Publication10
Alternative sequenceiVSP_021963222 – 469Missing in isoform 4. 1 PublicationAdd BLAST248
Alternative sequenceiVSP_021964358 – 469YRYPK…VPAHQ → PMKTWCSGWSPSSWNWRGRR MCWSFATRLSCAASWPTSLT RQLKSCPTSNAPCTQS in isoform 3. 1 PublicationAdd BLAST112

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY646092 mRNA. Translation: AAT72897.2.
AY707862 mRNA. Translation: AAU88258.1.
DQ350638 mRNA. Translation: ABC71307.1.
AY756065 mRNA. Translation: AAV32501.1.
AY756066 mRNA. Translation: AAV32502.1.
AY756067 mRNA. Translation: AAV32503.1.
AK155172 mRNA. Translation: BAE33093.1.
BC057594 mRNA. Translation: AAH57594.1.
CCDSiCCDS23541.1. [Q6DTY7-1]
RefSeqiNP_766607.3. NM_173019.5. [Q6DTY7-1]
XP_006512201.1. XM_006512138.2.
XP_017168891.1. XM_017313402.1. [Q6DTY7-6]
UniGeneiMm.132391.

Genome annotation databases

EnsembliENSMUST00000051873; ENSMUSP00000057197; ENSMUSG00000025648. [Q6DTY7-5]
ENSMUST00000198140; ENSMUSP00000142378; ENSMUSG00000025648. [Q6DTY7-1]
ENSMUST00000199591; ENSMUSP00000142992; ENSMUSG00000025648. [Q6DTY7-3]
GeneIDi270198.
KEGGimmu:270198.
UCSCiuc009rri.1. mouse. [Q6DTY7-1]
uc009rrk.1. mouse. [Q6DTY7-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY646092 mRNA. Translation: AAT72897.2.
AY707862 mRNA. Translation: AAU88258.1.
DQ350638 mRNA. Translation: ABC71307.1.
AY756065 mRNA. Translation: AAV32501.1.
AY756066 mRNA. Translation: AAV32502.1.
AY756067 mRNA. Translation: AAV32503.1.
AK155172 mRNA. Translation: BAE33093.1.
BC057594 mRNA. Translation: AAH57594.1.
CCDSiCCDS23541.1. [Q6DTY7-1]
RefSeqiNP_766607.3. NM_173019.5. [Q6DTY7-1]
XP_006512201.1. XM_006512138.2.
XP_017168891.1. XM_017313402.1. [Q6DTY7-6]
UniGeneiMm.132391.

3D structure databases

ProteinModelPortaliQ6DTY7.
SMRiQ6DTY7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057197.

PTM databases

iPTMnetiQ6DTY7.
PhosphoSitePlusiQ6DTY7.

Proteomic databases

PaxDbiQ6DTY7.
PeptideAtlasiQ6DTY7.
PRIDEiQ6DTY7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051873; ENSMUSP00000057197; ENSMUSG00000025648. [Q6DTY7-5]
ENSMUST00000198140; ENSMUSP00000142378; ENSMUSG00000025648. [Q6DTY7-1]
ENSMUST00000199591; ENSMUSP00000142992; ENSMUSG00000025648. [Q6DTY7-3]
GeneIDi270198.
KEGGimmu:270198.
UCSCiuc009rri.1. mouse. [Q6DTY7-1]
uc009rrk.1. mouse. [Q6DTY7-5]

Organism-specific databases

CTDi5210.
MGIiMGI:2687284. Pfkfb4.

Phylogenomic databases

eggNOGiKOG0234. Eukaryota.
COG0406. LUCA.
GeneTreeiENSGT00390000018751.
HOGENOMiHOG000181112.
HOVERGENiHBG005628.
InParanoidiQ6DTY7.
KOiK19030.
OMAiDCSNDEA.
OrthoDBiEOG091G0A43.
PhylomeDBiQ6DTY7.
TreeFamiTF313541.

Enzyme and pathway databases

ReactomeiR-MMU-70171. Glycolysis.

Miscellaneous databases

PROiQ6DTY7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025648.
ExpressionAtlasiQ6DTY7. baseline and differential.
GenevisibleiQ6DTY7. MM.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
PROSITEiPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF264_MOUSE
AccessioniPrimary (citable) accession number: Q6DTY7
Secondary accession number(s): Q5UD55
, Q5UD56, Q5UD57, Q5Y296, Q6PFE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 102 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.