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Protein

Serine/threonine-protein kinase MRCK gamma

Gene

CDC42BPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001ATPPROSITE-ProRule annotation1 Publication
Active sitei195 – 1951Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 859ATPPROSITE-ProRule annotationBy similarity
Zinc fingeri878 – 92750Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein serine/threonine kinase activity Source: UniProtKB
  4. small GTPase regulator activity Source: InterPro

GO - Biological processi

  1. actin cytoskeleton reorganization Source: UniProtKB
  2. intracellular signal transduction Source: InterPro
  3. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ6DT37.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MRCK gamma (EC:2.7.11.1)
Alternative name(s):
CDC42-binding protein kinase gamma
DMPK-like gamma
Myotonic dystrophy kinase-related CDC42-binding kinase gamma
Short name:
MRCK gamma
Short name:
MRCKG
Short name:
Myotonic dystrophy protein kinase-like gamma
Myotonic dystrophy protein kinase-like alpha
Gene namesi
Name:CDC42BPGImported
Synonyms:DMPK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:29829. CDC42BPG.

Subcellular locationi

Cytoplasm 1 Publication
Note: Concentrates at the leading edge of cells.

GO - Cellular componenti

  1. cell leading edge Source: UniProtKB
  2. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134901493.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15511551Serine/threonine-protein kinase MRCK gammaPRO_0000086397Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161Phosphoserine; by autocatalysisBy similarity
Modified residuei228 – 2281Phosphoserine; by autocatalysisBy similarity
Modified residuei234 – 2341Phosphothreonine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6DT37.
PaxDbiQ6DT37.
PRIDEiQ6DT37.

PTM databases

PhosphoSiteiQ6DT37.

Expressioni

Tissue specificityi

Expressed in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ6DT37.
CleanExiHS_CDC42BPG.
GenevestigatoriQ6DT37.

Organism-specific databases

HPAiHPA027382.
HPA061836.

Interactioni

Subunit structurei

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TBK1Q9UHD22EBI-689124,EBI-356402

Protein-protein interaction databases

BioGridi120719. 5 interactions.
IntActiQ6DT37. 4 interactions.
MINTiMINT-3976275.
STRINGi9606.ENSP00000345133.

Structurei

3D structure databases

ProteinModelPortaliQ6DT37.
SMRiQ6DT37. Positions 2-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 337267Protein kinasePROSITE-ProRule annotation1 PublicationAdd
BLAST
Domaini338 – 40871AGC-kinase C-terminalAdd
BLAST
Domaini947 – 1066120PHPROSITE-ProRule annotationAdd
BLAST
Domaini1092 – 1366275CNHPROSITE-ProRule annotationAdd
BLAST
Domaini1437 – 145014CRIBPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili406 – 678273Sequence AnalysisAdd
BLAST
Coiled coili730 – 80273Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 CNH domain.PROSITE-ProRule annotation
Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri878 – 92750Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118994.
HOGENOMiHOG000294133.
HOVERGENiHBG055933.
InParanoidiQ6DT37.
KOiK16307.
OMAiFRNHPFF.
OrthoDBiEOG7F511X.
PhylomeDBiQ6DT37.
TreeFamiTF313551.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR001180. Citron.
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR002219. PE/DAG-bd.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00109. C1. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DT37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERRLRALEQ LARGEAGGCP GLDGLLDLLL ALHHELSSGP LRRERSVAQF
60 70 80 90 100
LSWASPFVSK VKELRLQRDD FEILKVIGRG AFGEVTVVRQ RDTGQIFAMK
110 120 130 140 150
MLHKWEMLKR AETACFREER DVLVKGDSRW VTTLHYAFQD EEYLYLVMDY
160 170 180 190 200
YAGGDLLTLL SRFEDRLPPE LAQFYLAEMV LAIHSLHQLG YVHRDVKPDN
210 220 230 240 250
VLLDVNGHIR LADFGSCLRL NTNGMVDSSV AVGTPDYISP EILQAMEEGK
260 270 280 290 300
GHYGPQCDWW SLGVCAYELL FGETPFYAES LVETYGKIMN HEDHLQFPPD
310 320 330 340 350
VPDVPASAQD LIRQLLCRQE ERLGRGGLDD FRNHPFFEGV DWERLASSTA
360 370 380 390 400
PYIPELRGPM DTSNFDVDDD TLNHPGTLPP PSHGAFSGHH LPFVGFTYTS
410 420 430 440 450
GSHSPESSSE AWAALERKLQ CLEQEKVELS RKHQEALHAP TDHRELEQLR
460 470 480 490 500
KEVQTLRDRL PEMLRDKASL SQTDGPPAGS PGQDSDLRQE LDRLHRELAE
510 520 530 540 550
GRAGLQAQEQ ELCRAQGQQE ELLQRLQEAQ EREAATASQT RALSSQLEEA
560 570 580 590 600
RAAQRELEAQ VSSLSRQVTQ LQGQWEQRLE ESSQAKTIHT ASETNGMGPP
610 620 630 640 650
EGGPQEAQLR KEVAALREQL EQAHSHRPSG KEEALCQLQE ENRRLSREQE
660 670 680 690 700
RLEAELAQEQ ESKQRLEGER RETESNWEAQ LADILSWVND EKVSRGYLQA
710 720 730 740 750
LATKMAEELE SLRNVGTQTL PARPLDHQWK ARRLQKMEAS ARLELQSALE
760 770 780 790 800
AEIRAKQGLQ ERLTQVQEAQ LQAERRLQEA EKQSQALQQE LAMLREELRA
810 820 830 840 850
RGPVDTKPSN SLIPFLSFRS SEKDSAKDPG ISGEATRHGG EPDLRPEGRR
860 870 880 890 900
SLRMGAVFPR APTANTASTE GLPAKPGSHT LRPRSFPSPT KCLRCTSLML
910 920 930 940 950
GLGRQGLGCD ACGYFCHTTC APQAPPCPVP PDLLRTALGV HPETGTGTAY
960 970 980 990 1000
EGFLSVPRPS GVRRGWQRVF AALSDSRLLL FDAPDLRLSP PSGALLQVLD
1010 1020 1030 1040 1050
LRDPQFSATP VLASDVIHAQ SRDLPRIFRV TTSQLAVPPT TCTVLLLAES
1060 1070 1080 1090 1100
EGERERWLQV LGELQRLLLD ARPRPRPVYT LKEAYDNGLP LLPHTLCAAI
1110 1120 1130 1140 1150
LDQDRLALGT EEGLFVIHLR SNDIFQVGEC RRVQQLTLSP SAGLLVVLCG
1160 1170 1180 1190 1200
RGPSVRLFAL AELENIEVAG AKIPESRGCQ VLAAGSILQA RTPVLCVAVK
1210 1220 1230 1240 1250
RQVLCYQLGP GPGPWQRRIR ELQAPATVQS LGLLGDRLCV GAAGGFALYP
1260 1270 1280 1290 1300
LLNEAAPLAL GAGLVPEELP PSRGGLGEAL GAVELSLSEF LLLFTTAGIY
1310 1320 1330 1340 1350
VDGAGRKSRG HELLWPAAPM GWGYAAPYLT VFSENSIDVF DVRRAEWVQT
1360 1370 1380 1390 1400
VPLKKVRPLN PEGSLFLYGT EKVRLTYLRN QLAEKDEFDI PDLTDNSRRQ
1410 1420 1430 1440 1450
LFRTKSKRRF FFRVSEEQQK QQRREMLKDP FVRSKLISPP TNFNHLVHVG
1460 1470 1480 1490 1500
PANGRPGARD KSPAPEEKGR VARGSGPQRP HSFSEALRRP ASMGSEGLGG
1510 1520 1530 1540 1550
DADPMKRKPW TSLSSESVSC PQGSLSPATS LMQVSERPRS LPLSPELESS

P
Length:1,551
Mass (Da):172,459
Last modified:March 1, 2010 - v2
Checksum:iA6D042D5D11318D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti628 – 6281P → R in AAT67172 (PubMed:15194684).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti168 – 1681P → L.1 Publication
Corresponds to variant rs34454471 [ dbSNP | Ensembl ].
VAR_040840
Natural varianti280 – 2801S → F in a glioblastoma multiforme sample; somatic mutation. 1 Publication
VAR_040841
Natural varianti362 – 3621T → P.1 Publication
Corresponds to variant rs55688429 [ dbSNP | Ensembl ].
VAR_040842
Natural varianti537 – 5371A → D.1 Publication
Corresponds to variant rs34241745 [ dbSNP | Ensembl ].
VAR_040843
Natural varianti1135 – 11351Q → R.
Corresponds to variant rs3741395 [ dbSNP | Ensembl ].
VAR_057105

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY648038 mRNA. Translation: AAT67172.1.
AP001187 Genomic DNA. No translation available.
Y12337 mRNA. Translation: CAA73006.1.
CCDSiCCDS31601.1.
RefSeqiNP_059995.2. NM_017525.2.
UniGeneiHs.293590.

Genome annotation databases

EnsembliENST00000342711; ENSP00000345133; ENSG00000171219.
GeneIDi55561.
KEGGihsa:55561.
UCSCiuc001obs.4. human.

Polymorphism databases

DMDMi290457650.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY648038 mRNA. Translation: AAT67172.1.
AP001187 Genomic DNA. No translation available.
Y12337 mRNA. Translation: CAA73006.1.
CCDSiCCDS31601.1.
RefSeqiNP_059995.2. NM_017525.2.
UniGeneiHs.293590.

3D structure databases

ProteinModelPortaliQ6DT37.
SMRiQ6DT37. Positions 2-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120719. 5 interactions.
IntActiQ6DT37. 4 interactions.
MINTiMINT-3976275.
STRINGi9606.ENSP00000345133.

Chemistry

BindingDBiQ6DT37.
ChEMBLiCHEMBL5615.
GuidetoPHARMACOLOGYi1506.

PTM databases

PhosphoSiteiQ6DT37.

Polymorphism databases

DMDMi290457650.

Proteomic databases

MaxQBiQ6DT37.
PaxDbiQ6DT37.
PRIDEiQ6DT37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342711; ENSP00000345133; ENSG00000171219.
GeneIDi55561.
KEGGihsa:55561.
UCSCiuc001obs.4. human.

Organism-specific databases

CTDi55561.
GeneCardsiGC11M064591.
H-InvDBHIX0035842.
HGNCiHGNC:29829. CDC42BPG.
HPAiHPA027382.
HPA061836.
MIMi613991. gene.
neXtProtiNX_Q6DT37.
PharmGKBiPA134901493.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118994.
HOGENOMiHOG000294133.
HOVERGENiHBG055933.
InParanoidiQ6DT37.
KOiK16307.
OMAiFRNHPFF.
OrthoDBiEOG7F511X.
PhylomeDBiQ6DT37.
TreeFamiTF313551.

Enzyme and pathway databases

SignaLinkiQ6DT37.

Miscellaneous databases

ChiTaRSiCDC42BPG. human.
GenomeRNAii55561.
NextBioi60044.
PROiQ6DT37.
SOURCEiSearch...

Gene expression databases

BgeeiQ6DT37.
CleanExiHS_CDC42BPG.
GenevestigatoriQ6DT37.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR001180. Citron.
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR002219. PE/DAG-bd.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00109. C1. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of the human myotonic dystrophy kinase-related Cdc42-binding kinase gamma is regulated by promoter DNA methylation and Sp1 binding."
    Ng Y., Tan I., Lim L., Leung T.
    J. Biol. Chem. 279:34156-34164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Colon1 Publication.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The germinal centre kinase gene and a novel CDC25-like gene are located in the vicinity of the PYGM gene on 11q13."
    Kedra D., Seroussi E., Fransson I., Trifunovic J., Clark M., Lagercrantz J., Blennow E., Mehlin H., Dumanski J.
    Hum. Genet. 100:611-619(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-401.
  4. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-168; PHE-280; PRO-362 AND ASP-537.

Entry informationi

Entry nameiMRCKG_HUMAN
AccessioniPrimary (citable) accession number: Q6DT37
Secondary accession number(s): O00565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2005
Last sequence update: March 1, 2010
Last modified: March 3, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.