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Protein

Serine/threonine-protein kinase MRCK gamma

Gene

CDC42BPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation (By similarity).By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100ATPPROSITE-ProRule annotation1 Publication1
Active sitei195Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 85ATPPROSITE-ProRule annotationBy similarity9
Zinc fingeri878 – 927Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS15965-MONOMER.
SignaLinkiQ6DT37.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MRCK gamma (EC:2.7.11.1)
Alternative name(s):
CDC42-binding protein kinase gamma
DMPK-like gamma
Myotonic dystrophy kinase-related CDC42-binding kinase gamma
Short name:
MRCK gamma
Short name:
MRCKG
Short name:
Myotonic dystrophy protein kinase-like gamma
Myotonic dystrophy protein kinase-like alpha
Gene namesi
Name:CDC42BPGImported
Synonyms:DMPK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:29829. CDC42BPG.

Subcellular locationi

  • Cytoplasm 1 Publication

  • Note: Concentrates at the leading edge of cells.

GO - Cellular componenti

  • cell leading edge Source: UniProtKB
  • cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000171219.
PharmGKBiPA134901493.

Chemistry databases

ChEMBLiCHEMBL5615.
GuidetoPHARMACOLOGYi1506.

Polymorphism and mutation databases

BioMutaiCDC42BPG.
DMDMi290457650.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863971 – 1551Serine/threonine-protein kinase MRCK gammaAdd BLAST1551

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei216Phosphoserine; by autocatalysisBy similarity1
Modified residuei228Phosphoserine; by autocatalysisBy similarity1
Modified residuei234Phosphothreonine; by autocatalysisBy similarity1
Modified residuei1482PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6DT37.
MaxQBiQ6DT37.
PaxDbiQ6DT37.
PeptideAtlasiQ6DT37.
PRIDEiQ6DT37.

PTM databases

iPTMnetiQ6DT37.
PhosphoSitePlusiQ6DT37.

Expressioni

Tissue specificityi

Expressed in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000171219.
CleanExiHS_CDC42BPG.
GenevisibleiQ6DT37. HS.

Organism-specific databases

HPAiHPA027382.
HPA061836.

Interactioni

Subunit structurei

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TBK1Q9UHD22EBI-689124,EBI-356402

Protein-protein interaction databases

BioGridi120719. 5 interactors.
IntActiQ6DT37. 4 interactors.
MINTiMINT-3976275.
STRINGi9606.ENSP00000345133.

Chemistry databases

BindingDBiQ6DT37.

Structurei

3D structure databases

ProteinModelPortaliQ6DT37.
SMRiQ6DT37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 337Protein kinasePROSITE-ProRule annotation1 PublicationAdd BLAST267
Domaini338 – 408AGC-kinase C-terminalAdd BLAST71
Domaini947 – 1066PHPROSITE-ProRule annotationAdd BLAST120
Domaini1092 – 1366CNHPROSITE-ProRule annotationAdd BLAST275
Domaini1437 – 1450CRIBPROSITE-ProRule annotationAdd BLAST14

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili406 – 678Sequence analysisAdd BLAST273
Coiled coili730 – 802Sequence analysisAdd BLAST73

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 CNH domain.PROSITE-ProRule annotation
Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri878 – 927Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0612. Eukaryota.
ENOG410XR1Q. LUCA.
GeneTreeiENSGT00760000118994.
HOGENOMiHOG000294133.
HOVERGENiHBG055933.
InParanoidiQ6DT37.
KOiK16307.
OMAiFRNHPFF.
OrthoDBiEOG091G09EX.
PhylomeDBiQ6DT37.
TreeFamiTF313551.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR001180. CNH_dom.
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR033235. MRCK_gamma.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22988:SF22. PTHR22988:SF22. 1 hit.
PfamiPF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00109. C1. 1 hit.
SM00036. CNH. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DT37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERRLRALEQ LARGEAGGCP GLDGLLDLLL ALHHELSSGP LRRERSVAQF
60 70 80 90 100
LSWASPFVSK VKELRLQRDD FEILKVIGRG AFGEVTVVRQ RDTGQIFAMK
110 120 130 140 150
MLHKWEMLKR AETACFREER DVLVKGDSRW VTTLHYAFQD EEYLYLVMDY
160 170 180 190 200
YAGGDLLTLL SRFEDRLPPE LAQFYLAEMV LAIHSLHQLG YVHRDVKPDN
210 220 230 240 250
VLLDVNGHIR LADFGSCLRL NTNGMVDSSV AVGTPDYISP EILQAMEEGK
260 270 280 290 300
GHYGPQCDWW SLGVCAYELL FGETPFYAES LVETYGKIMN HEDHLQFPPD
310 320 330 340 350
VPDVPASAQD LIRQLLCRQE ERLGRGGLDD FRNHPFFEGV DWERLASSTA
360 370 380 390 400
PYIPELRGPM DTSNFDVDDD TLNHPGTLPP PSHGAFSGHH LPFVGFTYTS
410 420 430 440 450
GSHSPESSSE AWAALERKLQ CLEQEKVELS RKHQEALHAP TDHRELEQLR
460 470 480 490 500
KEVQTLRDRL PEMLRDKASL SQTDGPPAGS PGQDSDLRQE LDRLHRELAE
510 520 530 540 550
GRAGLQAQEQ ELCRAQGQQE ELLQRLQEAQ EREAATASQT RALSSQLEEA
560 570 580 590 600
RAAQRELEAQ VSSLSRQVTQ LQGQWEQRLE ESSQAKTIHT ASETNGMGPP
610 620 630 640 650
EGGPQEAQLR KEVAALREQL EQAHSHRPSG KEEALCQLQE ENRRLSREQE
660 670 680 690 700
RLEAELAQEQ ESKQRLEGER RETESNWEAQ LADILSWVND EKVSRGYLQA
710 720 730 740 750
LATKMAEELE SLRNVGTQTL PARPLDHQWK ARRLQKMEAS ARLELQSALE
760 770 780 790 800
AEIRAKQGLQ ERLTQVQEAQ LQAERRLQEA EKQSQALQQE LAMLREELRA
810 820 830 840 850
RGPVDTKPSN SLIPFLSFRS SEKDSAKDPG ISGEATRHGG EPDLRPEGRR
860 870 880 890 900
SLRMGAVFPR APTANTASTE GLPAKPGSHT LRPRSFPSPT KCLRCTSLML
910 920 930 940 950
GLGRQGLGCD ACGYFCHTTC APQAPPCPVP PDLLRTALGV HPETGTGTAY
960 970 980 990 1000
EGFLSVPRPS GVRRGWQRVF AALSDSRLLL FDAPDLRLSP PSGALLQVLD
1010 1020 1030 1040 1050
LRDPQFSATP VLASDVIHAQ SRDLPRIFRV TTSQLAVPPT TCTVLLLAES
1060 1070 1080 1090 1100
EGERERWLQV LGELQRLLLD ARPRPRPVYT LKEAYDNGLP LLPHTLCAAI
1110 1120 1130 1140 1150
LDQDRLALGT EEGLFVIHLR SNDIFQVGEC RRVQQLTLSP SAGLLVVLCG
1160 1170 1180 1190 1200
RGPSVRLFAL AELENIEVAG AKIPESRGCQ VLAAGSILQA RTPVLCVAVK
1210 1220 1230 1240 1250
RQVLCYQLGP GPGPWQRRIR ELQAPATVQS LGLLGDRLCV GAAGGFALYP
1260 1270 1280 1290 1300
LLNEAAPLAL GAGLVPEELP PSRGGLGEAL GAVELSLSEF LLLFTTAGIY
1310 1320 1330 1340 1350
VDGAGRKSRG HELLWPAAPM GWGYAAPYLT VFSENSIDVF DVRRAEWVQT
1360 1370 1380 1390 1400
VPLKKVRPLN PEGSLFLYGT EKVRLTYLRN QLAEKDEFDI PDLTDNSRRQ
1410 1420 1430 1440 1450
LFRTKSKRRF FFRVSEEQQK QQRREMLKDP FVRSKLISPP TNFNHLVHVG
1460 1470 1480 1490 1500
PANGRPGARD KSPAPEEKGR VARGSGPQRP HSFSEALRRP ASMGSEGLGG
1510 1520 1530 1540 1550
DADPMKRKPW TSLSSESVSC PQGSLSPATS LMQVSERPRS LPLSPELESS

P
Length:1,551
Mass (Da):172,459
Last modified:March 2, 2010 - v2
Checksum:iA6D042D5D11318D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti628P → R in AAT67172 (PubMed:15194684).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040840168P → L.1 PublicationCorresponds to variant rs34454471dbSNPEnsembl.1
Natural variantiVAR_040841280S → F in a glioblastoma multiforme sample; somatic mutation. 1 PublicationCorresponds to variant rs770462360dbSNPEnsembl.1
Natural variantiVAR_040842362T → P.1 PublicationCorresponds to variant rs55688429dbSNPEnsembl.1
Natural variantiVAR_040843537A → D.1 PublicationCorresponds to variant rs34241745dbSNPEnsembl.1
Natural variantiVAR_0571051135Q → R.Corresponds to variant rs3741395dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY648038 mRNA. Translation: AAT67172.1.
AP001187 Genomic DNA. No translation available.
Y12337 mRNA. Translation: CAA73006.1.
CCDSiCCDS31601.1.
RefSeqiNP_059995.2. NM_017525.2.
UniGeneiHs.293590.

Genome annotation databases

EnsembliENST00000342711; ENSP00000345133; ENSG00000171219.
GeneIDi55561.
KEGGihsa:55561.
UCSCiuc001obs.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY648038 mRNA. Translation: AAT67172.1.
AP001187 Genomic DNA. No translation available.
Y12337 mRNA. Translation: CAA73006.1.
CCDSiCCDS31601.1.
RefSeqiNP_059995.2. NM_017525.2.
UniGeneiHs.293590.

3D structure databases

ProteinModelPortaliQ6DT37.
SMRiQ6DT37.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120719. 5 interactors.
IntActiQ6DT37. 4 interactors.
MINTiMINT-3976275.
STRINGi9606.ENSP00000345133.

Chemistry databases

BindingDBiQ6DT37.
ChEMBLiCHEMBL5615.
GuidetoPHARMACOLOGYi1506.

PTM databases

iPTMnetiQ6DT37.
PhosphoSitePlusiQ6DT37.

Polymorphism and mutation databases

BioMutaiCDC42BPG.
DMDMi290457650.

Proteomic databases

EPDiQ6DT37.
MaxQBiQ6DT37.
PaxDbiQ6DT37.
PeptideAtlasiQ6DT37.
PRIDEiQ6DT37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342711; ENSP00000345133; ENSG00000171219.
GeneIDi55561.
KEGGihsa:55561.
UCSCiuc001obs.5. human.

Organism-specific databases

CTDi55561.
GeneCardsiCDC42BPG.
H-InvDBHIX0035842.
HGNCiHGNC:29829. CDC42BPG.
HPAiHPA027382.
HPA061836.
MIMi613991. gene.
neXtProtiNX_Q6DT37.
OpenTargetsiENSG00000171219.
PharmGKBiPA134901493.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0612. Eukaryota.
ENOG410XR1Q. LUCA.
GeneTreeiENSGT00760000118994.
HOGENOMiHOG000294133.
HOVERGENiHBG055933.
InParanoidiQ6DT37.
KOiK16307.
OMAiFRNHPFF.
OrthoDBiEOG091G09EX.
PhylomeDBiQ6DT37.
TreeFamiTF313551.

Enzyme and pathway databases

BioCyciZFISH:HS15965-MONOMER.
SignaLinkiQ6DT37.

Miscellaneous databases

ChiTaRSiCDC42BPG. human.
GenomeRNAii55561.
PROiQ6DT37.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171219.
CleanExiHS_CDC42BPG.
GenevisibleiQ6DT37. HS.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR001180. CNH_dom.
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR033235. MRCK_gamma.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR22988:SF22. PTHR22988:SF22. 1 hit.
PfamiPF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00109. C1. 1 hit.
SM00036. CNH. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMRCKG_HUMAN
AccessioniPrimary (citable) accession number: Q6DT37
Secondary accession number(s): O00565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 2, 2010
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.