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Q6DT37

- MRCKG_HUMAN

UniProt

Q6DT37 - MRCKG_HUMAN

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Protein
Serine/threonine-protein kinase MRCK gamma
Gene
CDC42BPG, DMPK2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation By similarity.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Magnesium.1 Publication

Enzyme regulationi

Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001ATP By similarity1 Publication
Active sitei195 – 1951Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 859ATP By similarityBy similarity
Zinc fingeri878 – 92750Phorbol-ester/DAG-type
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. protein binding Source: IntAct
  4. protein serine/threonine kinase activity Source: UniProtKB
  5. small GTPase regulator activity Source: InterPro

GO - Biological processi

  1. actin cytoskeleton reorganization Source: UniProtKB
  2. intracellular signal transduction Source: InterPro
  3. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

SignaLinkiQ6DT37.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase MRCK gamma (EC:2.7.11.1)
Alternative name(s):
CDC42-binding protein kinase gamma
DMPK-like gamma
Myotonic dystrophy kinase-related CDC42-binding kinase gamma
Short name:
MRCK gamma
Short name:
MRCKG
Short name:
Myotonic dystrophy protein kinase-like gamma
Myotonic dystrophy protein kinase-like alpha
Gene namesi
Synonyms:DMPK2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:29829. CDC42BPG.

Subcellular locationi

Cytoplasm
Note: Concentrates at the leading edge of cells.1 Publication

GO - Cellular componenti

  1. cell leading edge Source: UniProtKB
  2. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134901493.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15511551Serine/threonine-protein kinase MRCK gamma
PRO_0000086397Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161Phosphoserine; by autocatalysis By similarity1 Publication
Modified residuei228 – 2281Phosphoserine; by autocatalysis By similarity1 Publication
Modified residuei234 – 2341Phosphothreonine; by autocatalysis By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6DT37.
PaxDbiQ6DT37.
PRIDEiQ6DT37.

PTM databases

PhosphoSiteiQ6DT37.

Expressioni

Tissue specificityi

Expressed in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiQ6DT37.
CleanExiHS_CDC42BPG.
GenevestigatoriQ6DT37.

Organism-specific databases

HPAiHPA027382.

Interactioni

Subunit structurei

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
TBK1Q9UHD22EBI-689124,EBI-356402

Protein-protein interaction databases

BioGridi120719. 5 interactions.
IntActiQ6DT37. 4 interactions.
MINTiMINT-3976275.
STRINGi9606.ENSP00000345133.

Structurei

3D structure databases

ProteinModelPortaliQ6DT37.
SMRiQ6DT37. Positions 2-400.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 337267Protein kinase1 Publication
Add
BLAST
Domaini338 – 40871AGC-kinase C-terminal
Add
BLAST
Domaini947 – 1066120PH
Add
BLAST
Domaini1092 – 1366275CNH
Add
BLAST
Domaini1437 – 145014CRIB
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili406 – 678273 Reviewed prediction
Add
BLAST
Coiled coili730 – 80273 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 1 CNH domain.
Contains 1 CRIB domain.
Contains 1 PH domain.

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000294133.
HOVERGENiHBG055933.
InParanoidiQ6DT37.
KOiK16307.
OMAiTCAPQAP.
OrthoDBiEOG7F511X.
PhylomeDBiQ6DT37.
TreeFamiTF313551.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR001180. Citron.
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR017892. Pkinase_C.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
ProDomiPD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00109. C1. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DT37-1 [UniParc]FASTAAdd to Basket

« Hide

MERRLRALEQ LARGEAGGCP GLDGLLDLLL ALHHELSSGP LRRERSVAQF     50
LSWASPFVSK VKELRLQRDD FEILKVIGRG AFGEVTVVRQ RDTGQIFAMK 100
MLHKWEMLKR AETACFREER DVLVKGDSRW VTTLHYAFQD EEYLYLVMDY 150
YAGGDLLTLL SRFEDRLPPE LAQFYLAEMV LAIHSLHQLG YVHRDVKPDN 200
VLLDVNGHIR LADFGSCLRL NTNGMVDSSV AVGTPDYISP EILQAMEEGK 250
GHYGPQCDWW SLGVCAYELL FGETPFYAES LVETYGKIMN HEDHLQFPPD 300
VPDVPASAQD LIRQLLCRQE ERLGRGGLDD FRNHPFFEGV DWERLASSTA 350
PYIPELRGPM DTSNFDVDDD TLNHPGTLPP PSHGAFSGHH LPFVGFTYTS 400
GSHSPESSSE AWAALERKLQ CLEQEKVELS RKHQEALHAP TDHRELEQLR 450
KEVQTLRDRL PEMLRDKASL SQTDGPPAGS PGQDSDLRQE LDRLHRELAE 500
GRAGLQAQEQ ELCRAQGQQE ELLQRLQEAQ EREAATASQT RALSSQLEEA 550
RAAQRELEAQ VSSLSRQVTQ LQGQWEQRLE ESSQAKTIHT ASETNGMGPP 600
EGGPQEAQLR KEVAALREQL EQAHSHRPSG KEEALCQLQE ENRRLSREQE 650
RLEAELAQEQ ESKQRLEGER RETESNWEAQ LADILSWVND EKVSRGYLQA 700
LATKMAEELE SLRNVGTQTL PARPLDHQWK ARRLQKMEAS ARLELQSALE 750
AEIRAKQGLQ ERLTQVQEAQ LQAERRLQEA EKQSQALQQE LAMLREELRA 800
RGPVDTKPSN SLIPFLSFRS SEKDSAKDPG ISGEATRHGG EPDLRPEGRR 850
SLRMGAVFPR APTANTASTE GLPAKPGSHT LRPRSFPSPT KCLRCTSLML 900
GLGRQGLGCD ACGYFCHTTC APQAPPCPVP PDLLRTALGV HPETGTGTAY 950
EGFLSVPRPS GVRRGWQRVF AALSDSRLLL FDAPDLRLSP PSGALLQVLD 1000
LRDPQFSATP VLASDVIHAQ SRDLPRIFRV TTSQLAVPPT TCTVLLLAES 1050
EGERERWLQV LGELQRLLLD ARPRPRPVYT LKEAYDNGLP LLPHTLCAAI 1100
LDQDRLALGT EEGLFVIHLR SNDIFQVGEC RRVQQLTLSP SAGLLVVLCG 1150
RGPSVRLFAL AELENIEVAG AKIPESRGCQ VLAAGSILQA RTPVLCVAVK 1200
RQVLCYQLGP GPGPWQRRIR ELQAPATVQS LGLLGDRLCV GAAGGFALYP 1250
LLNEAAPLAL GAGLVPEELP PSRGGLGEAL GAVELSLSEF LLLFTTAGIY 1300
VDGAGRKSRG HELLWPAAPM GWGYAAPYLT VFSENSIDVF DVRRAEWVQT 1350
VPLKKVRPLN PEGSLFLYGT EKVRLTYLRN QLAEKDEFDI PDLTDNSRRQ 1400
LFRTKSKRRF FFRVSEEQQK QQRREMLKDP FVRSKLISPP TNFNHLVHVG 1450
PANGRPGARD KSPAPEEKGR VARGSGPQRP HSFSEALRRP ASMGSEGLGG 1500
DADPMKRKPW TSLSSESVSC PQGSLSPATS LMQVSERPRS LPLSPELESS 1550
P 1551
Length:1,551
Mass (Da):172,459
Last modified:March 2, 2010 - v2
Checksum:iA6D042D5D11318D7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti168 – 1681P → L.1 Publication
Corresponds to variant rs34454471 [ dbSNP | Ensembl ].
VAR_040840
Natural varianti280 – 2801S → F in a glioblastoma multiforme sample; somatic mutation. 1 Publication
VAR_040841
Natural varianti362 – 3621T → P.1 Publication
Corresponds to variant rs55688429 [ dbSNP | Ensembl ].
VAR_040842
Natural varianti537 – 5371A → D.1 Publication
Corresponds to variant rs34241745 [ dbSNP | Ensembl ].
VAR_040843
Natural varianti1135 – 11351Q → R.
Corresponds to variant rs3741395 [ dbSNP | Ensembl ].
VAR_057105

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti628 – 6281P → R in AAT67172. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY648038 mRNA. Translation: AAT67172.1.
AP001187 Genomic DNA. No translation available.
Y12337 mRNA. Translation: CAA73006.1.
CCDSiCCDS31601.1.
RefSeqiNP_059995.2. NM_017525.2.
UniGeneiHs.293590.

Genome annotation databases

EnsembliENST00000342711; ENSP00000345133; ENSG00000171219.
GeneIDi55561.
KEGGihsa:55561.
UCSCiuc001obs.4. human.

Polymorphism databases

DMDMi290457650.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY648038 mRNA. Translation: AAT67172.1 .
AP001187 Genomic DNA. No translation available.
Y12337 mRNA. Translation: CAA73006.1 .
CCDSi CCDS31601.1.
RefSeqi NP_059995.2. NM_017525.2.
UniGenei Hs.293590.

3D structure databases

ProteinModelPortali Q6DT37.
SMRi Q6DT37. Positions 2-400.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120719. 5 interactions.
IntActi Q6DT37. 4 interactions.
MINTi MINT-3976275.
STRINGi 9606.ENSP00000345133.

Chemistry

ChEMBLi CHEMBL5615.
GuidetoPHARMACOLOGYi 1506.

PTM databases

PhosphoSitei Q6DT37.

Polymorphism databases

DMDMi 290457650.

Proteomic databases

MaxQBi Q6DT37.
PaxDbi Q6DT37.
PRIDEi Q6DT37.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000342711 ; ENSP00000345133 ; ENSG00000171219 .
GeneIDi 55561.
KEGGi hsa:55561.
UCSCi uc001obs.4. human.

Organism-specific databases

CTDi 55561.
GeneCardsi GC11M064591.
H-InvDB HIX0035842.
HGNCi HGNC:29829. CDC42BPG.
HPAi HPA027382.
MIMi 613991. gene.
neXtProti NX_Q6DT37.
PharmGKBi PA134901493.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000294133.
HOVERGENi HBG055933.
InParanoidi Q6DT37.
KOi K16307.
OMAi TCAPQAP.
OrthoDBi EOG7F511X.
PhylomeDBi Q6DT37.
TreeFami TF313551.

Enzyme and pathway databases

SignaLinki Q6DT37.

Miscellaneous databases

ChiTaRSi CDC42BPG. human.
GenomeRNAii 55561.
NextBioi 60044.
PROi Q6DT37.
SOURCEi Search...

Gene expression databases

Bgeei Q6DT37.
CleanExi HS_CDC42BPG.
Genevestigatori Q6DT37.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR001180. Citron.
IPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR014930. Myotonic_dystrophy_kinase_coil.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR017892. Pkinase_C.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00130. C1_1. 1 hit.
PF00780. CNH. 1 hit.
PF08826. DMPK_coil. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view ]
ProDomi PD011252. Myotonic_dystrophy_kinase_coil. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00109. C1. 1 hit.
SM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50219. CNH. 1 hit.
PS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of the human myotonic dystrophy kinase-related Cdc42-binding kinase gamma is regulated by promoter DNA methylation and Sp1 binding."
    Ng Y., Tan I., Lim L., Leung T.
    J. Biol. Chem. 279:34156-34164(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Colon.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The germinal centre kinase gene and a novel CDC25-like gene are located in the vicinity of the PYGM gene on 11q13."
    Kedra D., Seroussi E., Fransson I., Trifunovic J., Clark M., Lagercrantz J., Blennow E., Mehlin H., Dumanski J.
    Hum. Genet. 100:611-619(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-401.
  4. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-168; PHE-280; PRO-362 AND ASP-537.

Entry informationi

Entry nameiMRCKG_HUMAN
AccessioniPrimary (citable) accession number: Q6DT37
Secondary accession number(s): O00565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: March 2, 2010
Last modified: September 3, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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