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Q6DNG6 (SIA8A_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 6, 2013. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
EC=2.4.99.8
Alternative name(s):
Alpha-2,8-sialyltransferase 8A
Ganglioside GD3 synthase
Sialyltransferase 8A
Short name=SIAT8-A
Sialyltransferase St8Sia I
Short name=ST8SiaI
Gene names
Name:st8sia1
Synonyms:st8siaI
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the production of gangliosides GD3 and GT3 from GM3. Gangliosides are a subfamily of complex glycosphingolipids that contain one or more residues of sialic acid; glycosphingolipids are required for convergence extension movements during early development By similarity. UniProtKB Q6ZXA0

Catalytic activity

CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R = CMP + alpha-N-acetylneuraminyl-(2->8)-alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-R. UniProtKB Q6ZXA0

Pathway

Protein modification; protein glycosylation.

Lipid metabolism; sphingolipid metabolism. UniProtKB Q6ZXA0

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity UniProtKB Q6ZXA0.

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
PRO_0000376860

Regions

Topological domain1 – 1515Cytoplasmic Potential
Transmembrane16 – 3621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain37 – 345309Lumenal Potential

Amino acid modifications

Glycosylation591N-linked (GlcNAc...) Potential
Glycosylation1071N-linked (GlcNAc...) Potential
Glycosylation2331N-linked (GlcNAc...) Potential
Disulfide bond126 ↔ 275 By similarity UniProtKB Q92185

Sequences

Sequence LengthMass (Da)Tools
Q6DNG6 [UniParc].

Last modified September 13, 2004. Version 2.
Checksum: 222B64EA41A2C62F

FASTA34539,831
        10         20         30         40         50         60 
MKLQGSRMWL CPRTRLPVGA SALGFLILCW LYVFPGYRLP GHKEMVREVL RFGPGWRKNR 

        70         80         90        100        110        120 
TEMDSFRKLL QDCCDPPHLF SLTKVNTPLG ENLWFDGEFF HSLTIDNSTR SLFPQDTPFK 

       130        140        150        160        170        180 
LPLKRCSVVG NGGILKNSRC GEQIDEADFV MRCNLPPLSR EYTEDVGTRT QLVTVNPSII 

       190        200        210        220        230        240 
DKRYQNLLWS RKSFVENLRV YQQSYVYMPA FSTKRGTDPS LRVYYTLADF GTNQTVLFAN 

       250        260        270        280        290        300 
PNFLRNVGKF WKSRGIHSKR LSTGLFMVSA ALSLCEEVTI YGFWPFQMDL GGRYISHHYY 

       310        320        330        340 
DNTLPLSGVH AMPEEFLQLW LLHKSGVLQM QLDQCKKDVS SQKPH

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of alpha2,8-sialyltransferase (ST8Sia I, GD3 Synthase) in Xenopus."
Rimoldi S., Papis E., Bernardini G., Prati M., Gornati R.
Mol. Cell. Biochem. 301:143-153(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY652775 mRNA. Translation: AAT67042.2.
BC170804 mRNA. Translation: AAI70804.1.
BC170806 mRNA. Translation: AAI70806.1.
RefSeqNP_001003659.1. NM_001003659.1.
UniGeneStr.24556.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID444887.
KEGGxtr:444887.

Organism-specific databases

CTD6489.
XenbaseXB-GENE-5824739. st8sia1.

Phylogenomic databases

KOK03371.

Enzyme and pathway databases

BRENDA2.4.99.8. 8483.
UniPathwayUPA00222.
UPA00378.

Family and domain databases

InterProIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSIA8A_XENTR
AccessionPrimary (citable) accession number: Q6DNG6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: September 13, 2004
Last modified: February 6, 2013
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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