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Q6DNA2 (ALG3_PICPG) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
EC=2.4.1.258
Alternative name(s):
Asparagine-linked glycosylation protein 6
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Gene names
Name:ALG3
Ordered Locus Names:PAS_chr4_0712
OrganismPichia pastoris (strain GS115 / ATCC 20864) (Yeast) [Complete proteome]
Taxonomic identifier644223 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Komagataella

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc(2)-PP-Dol By similarity. Ref.1

Catalytic activity

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the ALG3 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functiontransferase activity, transferring hexosyl groups

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase
PRO_0000350933

Regions

Topological domain1 – 3333Lumenal Potential
Transmembrane34 – 5421Helical; Potential
Topological domain55 – 8329Cytoplasmic Potential
Transmembrane84 – 10421Helical; Potential
Topological domain105 – 12016Lumenal Potential
Transmembrane121 – 14121Helical; Potential
Topological domain142 – 16019Cytoplasmic Potential
Transmembrane161 – 18121Helical; Potential
Topological domain182 – 21130Lumenal Potential
Transmembrane212 – 23221Helical; Potential
Topological domain233 – 2397Cytoplasmic Potential
Transmembrane240 – 26021Helical; Potential
Topological domain261 – 30646Lumenal Potential
Transmembrane307 – 32721Helical; Potential
Topological domain328 – 36538Cytoplasmic Potential
Transmembrane366 – 38520Helical; Potential
Topological domain386 – 40722Lumenal Potential
Transmembrane408 – 42821Helical; Potential
Topological domain429 – 4324Cytoplasmic Potential
Transmembrane433 – 45321Helical; Potential
Topological domain454 – 46512Lumenal Potential

Experimental info

Sequence conflict1881Y → S in AAT72073. Ref.1
Sequence conflict198 – 2036FILSLI → LSVPD in AAT72073. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6DNA2 [UniParc].

Last modified September 1, 2009. Version 2.
Checksum: 588C159AC7827F1C

FASTA46553,029
        10         20         30         40         50         60 
MPPIEPAERP KLTLKNVIGD LVALIQNVLF NPDFSVFVAP LLWLADSIVI KVIIGTVSYT 

        70         80         90        100        110        120 
DIDFSSYMQQ IFKIRQGELD YSNIFGDTGP LVYPAGHVHA YSVLSWYSDG GEDVSFVQQA 

       130        140        150        160        170        180 
FGWLYLGCLL LSISSYFFSG LGKIPPVYFV LLVASKRLHS IFVLRLFNDC LTTFLMLATI 

       190        200        210        220        230        240 
IILQQASYWR KDGTTIPFIL SLIAADTYSL AISVKMNALL YLPAFLLLIY LICDENLIKA 

       250        260        270        280        290        300 
LAPVLVLILV QVGVGYSFIL PLHYDDQANE IRSAYFRQAF DFSRQFLYKW TVNWRFLSQE 

       310        320        330        340        350        360 
TFNNVHFHQL LFALHIITLV LFILKFLSPK NIGKPLGRFV LDIFKFWKPT LSPTNIINDP 

       370        380        390        400        410        420 
ERSPDFVYTV MATTNLIGVL FARSLHYQFL SWYAFSLPYL LYKARLNFIA SIIVYAAHEY 

       430        440        450        460 
CWLVFPATEQ SSALLVSILL LILILIFTNE QLFPSQSVPA EKKNT

« Hide

References

« Hide 'large scale' references
[1]"Functional analysis of the ALG3 gene encoding the Dol-P-Man: Man5GlcNAc2-PP-Dol mannosyltransferase enzyme of P. pastoris."
Davidson R.C., Nett J.H., Renfer E., Li H., Stadheim T.A., Miller B.J., Miele R.G., Hamilton S.R., Choi B.-K., Mitchell T.I., Wildt S.
Glycobiology 14:399-407(2004) [PubMed: 15033937] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"Genome sequence of the recombinant protein production host Pichia pastoris."
De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S., Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.
Nat. Biotechnol. 27:561-566(2009) [PubMed: 19465926] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GS115 / ATCC 20864.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY653304 Genomic DNA. Translation: AAT72073.1.
FN392322 Genomic DNA. Translation: CAY71973.1.
RefSeqXP_002494152.1. XM_002494107.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ6DNA2.

Protein family/group databases

CAZyGT58. Glycosyltransferase Family 58.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8200607.
GenomeReviewsGene locus PAS_chr4_0712 in contig FN392322_GR.
KEGGppa:PAS_chr4_0712.

Phylogenomic databases

OrthoDBEOG4MPN04.

Family and domain databases

InterProIPR007873. Glycosyltransferase_ALG3.
[Graphical view]
KOK03845.
PANTHERPTHR12646. ALG3. 1 hit.
PfamPF05208. ALG3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALG3_PICPG
AccessionPrimary (citable) accession number: Q6DNA2
Secondary accession number(s): C4R8P8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: September 1, 2009
Last modified: December 14, 2011
This is version 39 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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