IQ motif and SEC7 domain-containing protein 1

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Preferred order of sections

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

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Q6DN90 (IQEC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 73. History...

Clusters with 100%, 90%, 50% identity |

Documents (6) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
IQ motif and SEC7 domain-containing protein 1

Alternative name(s):
ADP-ribosylation factors guanine nucleotide-exchange protein 100
ADP-ribosylation factors guanine nucleotide-exchange protein 2
Brefeldin-resistant Arf-GEF 2 protein

Gene names

Name:	IQSEC1
Synonyms:	ARFGEP100, BRAG2, KIAA0763

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Sequence length	963 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function	In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin. Ref.1 Ref.4
Subunit structure	Interacts with ARF6. Ref.4
Subcellular location	Cytoplasm. Nucleus. Note: At steady state, may be preferentially cytosolic. Ref.4
Tissue specificity	Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis. Ref.2 Ref.4
Sequence similarities	Belongs to the BRAG family. Contains 1 IQ domain. Contains 1 PH domain. Contains 1 SEC7 domain.

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cell junction Inferred from direct assay. Source: HPA cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome nucleolus Inferred from direct assay. Source: HPA
Molecular function	ARF guanyl-nucleotide exchange factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 963

963

IQ motif and SEC7 domain-containing protein 1

PRO_0000245606

Domain

134 – 163

Domain

517 – 710

194

SEC7

Domain

774 – 866

Coiled coil

848 – 879

Potential

Modified residue

107

Phosphoserine By similarity

Modified residue

180

Phosphoserine Ref.6 Ref.7

Modified residue

362

Phosphothreonine Ref.5

Modified residue

512

Phosphoserine Ref.6 Ref.7 Ref.8

Modified residue

515

Phosphoserine By similarity

Alternative sequence

1 – 122

122

Missing in isoform 2.

VSP_019758

Natural variant

640

P → S.
Corresponds to variant rs35319679 [ dbSNP | Ensembl ].

VAR_051927

Natural variant

882

V → I.
Corresponds to variant rs17541405 [ dbSNP | Ensembl ].

VAR_027004

Sequence conflict

934 – 936

KRG → VHH Ref.3

963

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform 1 (BRAG2b) [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 5B31F9918F9CFF11 Show »	FASTA	963	108,314
10 20 30 40 50 60 MWCLHCNSER TQSLLELELD SGVEGEAPSS ETGTSLDSPS AYPQGPLVPG SSLSPDHYEH 70 80 90 100 110 120 TSVGAYGLYS GPPGQQQRTR RPKLQHSTSI LRKQAEEEAI KRSRSLSESY ELSSDLQDKQ 130 140 150 160 170 180 VEMLERKYGG RLVTRHAART IQTAFRQYQM NKNFERLRSS MSENRMSRRI VLSNMRMQFS 190 200 210 220 230 240 FEGPEKVHSS YFEGKQVSVT NDGSQLGALV SPECGDLSEP TTLKSPAPSS DFADAITELE 250 260 270 280 290 300 DAFSRQVKSL AESIDDALNC RSLHTEEAPA LDAARARDTE PQTALHGMDH RKLDEMTASY 310 320 330 340 350 360 SDVTLYIDEE ELSPPLPLSQ AGDRPSSTES DLRLRAGGAA PDYWALAHKE DKADTDTSCR 370 380 390 400 410 420 STPSLERQEQ RLRVEHLPLL TIEPPSDSSV DLSDRSERGS LKRQSAYERS LGGQQGSPKH 430 440 450 460 470 480 GPHSGAPKSL PREEPELRPR PPRPLDSHLA INGSANRQSK SESDYSDGDN DSINSTSNSN 490 500 510 520 530 540 DTINCSSESS SRDSLREQTL SKQTYHKEAR NSWDSPAFSN DVIRKRHYRI GLNLFNKKPE 550 560 570 580 590 600 KGVQYLIERG FVPDTPVGVA HFLLQRKGLS RQMIGEFLGN RQKQFNRDVL DCVVDEMDFS 610 620 630 640 650 660 TMELDEALRK FQAHIRVQGE AQKVERLIEA FSQRYCICNP GVVRQFRNPD TIFILAFAII 670 680 690 700 710 720 LLNTDMYSPN VKPERKMKLE DFIKNLRGVD DGEDIPREML MGIYERIRKR ELKTNEDHVS 730 740 750 760 770 780 QVQKVEKLIV GKKPIGSLHP GLGCVLSLPH RRLVCYCRLF EVPDPNKPQK LGLHQREIFL 790 800 810 820 830 840 FNDLLVVTKI FQKKKNSVTY SFRQSFSLYG MQVLLFENQY YPNGIRLTSS VPGADIKVLI 850 860 870 880 890 900 NFNAPNPQDR KKFTDDLRES IAEVQEMEKH RIESELEKQK GVVRPSMSQC SSLKKESGNG 910 920 930 940 950 960 TLSRACLDDS YASGEGLKRS ALSSSLRDLS EAGKRGRRSS AGSLESNVEF QPFEPLQPSV LCS « Hide
Isoform 2 (BRAG2a) [UniParc]. Checksum: 504A8DDC71EB8B71 Show »	FASTA	841	94,914
10 20 30 40 50 60 MLERKYGGRL VTRHAARTIQ TAFRQYQMNK NFERLRSSMS ENRMSRRIVL SNMRMQFSFE 70 80 90 100 110 120 GPEKVHSSYF EGKQVSVTND GSQLGALVSP ECGDLSEPTT LKSPAPSSDF ADAITELEDA 130 140 150 160 170 180 FSRQVKSLAE SIDDALNCRS LHTEEAPALD AARARDTEPQ TALHGMDHRK LDEMTASYSD 190 200 210 220 230 240 VTLYIDEEEL SPPLPLSQAG DRPSSTESDL RLRAGGAAPD YWALAHKEDK ADTDTSCRST 250 260 270 280 290 300 PSLERQEQRL RVEHLPLLTI EPPSDSSVDL SDRSERGSLK RQSAYERSLG GQQGSPKHGP 310 320 330 340 350 360 HSGAPKSLPR EEPELRPRPP RPLDSHLAIN GSANRQSKSE SDYSDGDNDS INSTSNSNDT 370 380 390 400 410 420 INCSSESSSR DSLREQTLSK QTYHKEARNS WDSPAFSNDV IRKRHYRIGL NLFNKKPEKG 430 440 450 460 470 480 VQYLIERGFV PDTPVGVAHF LLQRKGLSRQ MIGEFLGNRQ KQFNRDVLDC VVDEMDFSTM 490 500 510 520 530 540 ELDEALRKFQ AHIRVQGEAQ KVERLIEAFS QRYCICNPGV VRQFRNPDTI FILAFAIILL 550 560 570 580 590 600 NTDMYSPNVK PERKMKLEDF IKNLRGVDDG EDIPREMLMG IYERIRKREL KTNEDHVSQV 610 620 630 640 650 660 QKVEKLIVGK KPIGSLHPGL GCVLSLPHRR LVCYCRLFEV PDPNKPQKLG LHQREIFLFN 670 680 690 700 710 720 DLLVVTKIFQ KKKNSVTYSF RQSFSLYGMQ VLLFENQYYP NGIRLTSSVP GADIKVLINF 730 740 750 760 770 780 NAPNPQDRKK FTDDLRESIA EVQEMEKHRI ESELEKQKGV VRPSMSQCSS LKKESGNGTL 790 800 810 820 830 840 SRACLDDSYA SGEGLKRSAL SSSLRDLSEA GKRGRRSSAG SLESNVEFQP FEPLQPSVLC S « Hide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins." Dunphy J.L., Moravec R., Ly K., Lasell T.K., Melancon P., Casanova J.E. Curr. Biol. 16:315-320(2006) [PubMed: 16461286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-936 (ISOFORM 2). Tissue: Placenta.
[4]	"ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6." Someya A., Sata M., Takeda K., Pacheco-Rodriguez G., Ferrans V.J., Moss J., Vaughan M. Proc. Natl. Acad. Sci. U.S.A. 98:2413-2418(2001) [PubMed: 11226253] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ARF6.
[5]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-362, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180 AND SER-512, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180 AND SER-512, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[8]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

AY653734 mRNA. Translation: AAT72063.1.
AB018306 mRNA. Translation: BAA34483.2.
BC010267 mRNA. Translation: AAH10267.1.

IPI

IPI00457114.
IPI00760711.

RefSeq

NP_001127854.1. NM_001134382.1.
NP_055684.3. NM_014869.4.

UniGene

Hs.475506.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3QWM	X-ray	2.39	A	743-880	[»]

ProteinModelPortal

Q6DN90.

SMR

Q6DN90. Positions 522-887.

ModBase

Search...

IntAct

Q6DN90. 2 interactions.

STRING

Q6DN90.

PhosphoSite

Q6DN90.

DMDM

74748429.

PRIDE

Q6DN90.

StructuralBiologyKnowledgebase

Search...

Ensembl

ENST00000273221; ENSP00000273221; ENSG00000144711.

GeneID

9922.

KEGG

hsa:9922.

UCSC

uc003bxt.1. human.
uc003bxu.2. human.

CTD

9922.

GeneCards

GC03M012938.

H-InvDB

HIX0200468.

HGNC

HGNC:29112. IQSEC1.

HPA

HPA038143.

MIM

610166. gene.

neXtProt

NX_Q6DN90.

HUGE

Search...

GenAtlas

Search...

eggNOG

prNOG16108.

GeneTree

ENSGT00590000083058.

HOVERGEN

HBG056324.

InParanoid

Q6DN90.

OrthoDB

EOG48GW2K.

PhylomeDB

Q6DN90.

Pathway_Interaction_DB

arf6cyclingpathway. Arf6 signaling events.

ArrayExpress

Q6DN90.

Bgee

Q6DN90.

CleanEx

HS_IQSEC1.

Genevestigator

Q6DN90.

GermOnline

ENSG00000144711. Homo sapiens.

InterPro

IPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR000904. Sec7.
IPR023394. SEC7_alpha_orthog.
[Graphical view]

Gene3D

G3DSA:1.10.1000.11. G3DSA:1.10.1000.11. 1 hit.
G3DSA:2.30.29.30. PH_type. 1 hit.

K12495.

Pfam

PF01369. Sec7. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]

SUPFAM

SSF48425. Sec7. 1 hit.

PROSITE

PS50096. IQ. 1 hit.
PS50003. PH_DOMAIN. False negative.
PS50190. SEC7. 1 hit.
[Graphical view]

ProtoNet

Search...

NextBio

37432.

SOURCE

Search...

Entry name

IQEC1_HUMAN

Accession

Primary (citable) accession number: Q6DN90
Secondary accession number(s): O94863, Q96D85

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6DN90-1)

Also known as: BRAG2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6DN90-2)

Also known as: BRAG2a;

The sequence of this isoform differs from the canonical sequence as follows:
1-122: Missing.

Note: No experimental confirmation available.