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Q6DN90 (IQEC1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
IQ motif and SEC7 domain-containing protein 1
Alternative name(s):
ADP-ribosylation factors guanine nucleotide-exchange protein 100
ADP-ribosylation factors guanine nucleotide-exchange protein 2
Brefeldin-resistant Arf-GEF 2 protein
Gene names
Name:IQSEC1
Synonyms:ARFGEP100, BRAG2, KIAA0763
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length963 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin. Ref.1 Ref.4

Subunit structure

Interacts with ARF6. Ref.4

Subcellular location

Cytoplasm. Nucleus. Note: At steady state, may be preferentially cytosolic. Ref.4

Tissue specificity

Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis. Ref.2 Ref.4

Sequence similarities

Belongs to the BRAG family.

Contains 1 IQ domain.

Contains 1 PH domain.

Contains 1 SEC7 domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6DN90-1)

Also known as: BRAG2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6DN90-2)

Also known as: BRAG2a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 963963IQ motif and SEC7 domain-containing protein 1
PRO_0000245606

Regions

Domain134 – 16330IQ
Domain517 – 710194SEC7
Domain774 – 86693PH
Coiled coil848 – 87932 Potential

Amino acid modifications

Modified residue1801Phosphoserine Ref.8
Modified residue5121Phosphoserine Ref.5 Ref.8
Modified residue5151Phosphoserine By similarity

Natural variations

Alternative sequence1 – 122122Missing in isoform 2.
VSP_019758
Natural variant6401P → S.
Corresponds to variant rs35319679 [ dbSNP | Ensembl ].
VAR_051927
Natural variant8821V → I.
Corresponds to variant rs17541405 [ dbSNP | Ensembl ].
VAR_027004

Experimental info

Sequence conflict934 – 9363KRG → VHH Ref.3

Secondary structure

................................................. 963
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (BRAG2b) [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 5B31F9918F9CFF11

FASTA963108,314
        10         20         30         40         50         60 
MWCLHCNSER TQSLLELELD SGVEGEAPSS ETGTSLDSPS AYPQGPLVPG SSLSPDHYEH 

        70         80         90        100        110        120 
TSVGAYGLYS GPPGQQQRTR RPKLQHSTSI LRKQAEEEAI KRSRSLSESY ELSSDLQDKQ 

       130        140        150        160        170        180 
VEMLERKYGG RLVTRHAART IQTAFRQYQM NKNFERLRSS MSENRMSRRI VLSNMRMQFS 

       190        200        210        220        230        240 
FEGPEKVHSS YFEGKQVSVT NDGSQLGALV SPECGDLSEP TTLKSPAPSS DFADAITELE 

       250        260        270        280        290        300 
DAFSRQVKSL AESIDDALNC RSLHTEEAPA LDAARARDTE PQTALHGMDH RKLDEMTASY 

       310        320        330        340        350        360 
SDVTLYIDEE ELSPPLPLSQ AGDRPSSTES DLRLRAGGAA PDYWALAHKE DKADTDTSCR 

       370        380        390        400        410        420 
STPSLERQEQ RLRVEHLPLL TIEPPSDSSV DLSDRSERGS LKRQSAYERS LGGQQGSPKH 

       430        440        450        460        470        480 
GPHSGAPKSL PREEPELRPR PPRPLDSHLA INGSANRQSK SESDYSDGDN DSINSTSNSN 

       490        500        510        520        530        540 
DTINCSSESS SRDSLREQTL SKQTYHKEAR NSWDSPAFSN DVIRKRHYRI GLNLFNKKPE 

       550        560        570        580        590        600 
KGVQYLIERG FVPDTPVGVA HFLLQRKGLS RQMIGEFLGN RQKQFNRDVL DCVVDEMDFS 

       610        620        630        640        650        660 
TMELDEALRK FQAHIRVQGE AQKVERLIEA FSQRYCICNP GVVRQFRNPD TIFILAFAII 

       670        680        690        700        710        720 
LLNTDMYSPN VKPERKMKLE DFIKNLRGVD DGEDIPREML MGIYERIRKR ELKTNEDHVS 

       730        740        750        760        770        780 
QVQKVEKLIV GKKPIGSLHP GLGCVLSLPH RRLVCYCRLF EVPDPNKPQK LGLHQREIFL 

       790        800        810        820        830        840 
FNDLLVVTKI FQKKKNSVTY SFRQSFSLYG MQVLLFENQY YPNGIRLTSS VPGADIKVLI 

       850        860        870        880        890        900 
NFNAPNPQDR KKFTDDLRES IAEVQEMEKH RIESELEKQK GVVRPSMSQC SSLKKESGNG 

       910        920        930        940        950        960 
TLSRACLDDS YASGEGLKRS ALSSSLRDLS EAGKRGRRSS AGSLESNVEF QPFEPLQPSV 


LCS 

« Hide

Isoform 2 (BRAG2a) [UniParc].

Checksum: 504A8DDC71EB8B71
Show »

FASTA84194,914

References

« Hide 'large scale' references
[1]"The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins."
Dunphy J.L., Moravec R., Ly K., Lasell T.K., Melancon P., Casanova J.E.
Curr. Biol. 16:315-320(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-936 (ISOFORM 2).
Tissue: Placenta.
[4]"ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6."
Someya A., Sata M., Takeda K., Pacheco-Rodriguez G., Ferrans V.J., Moss J., Vaughan M.
Proc. Natl. Acad. Sci. U.S.A. 98:2413-2418(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ARF6.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180 AND SER-512, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY653734 mRNA. Translation: AAT72063.1.
AB018306 mRNA. Translation: BAA34483.2.
BC010267 mRNA. Translation: AAH10267.1.
CCDSCCDS33703.1. [Q6DN90-1]
RefSeqNP_001127854.1. NM_001134382.2.
NP_055684.3. NM_014869.6. [Q6DN90-1]
UniGeneHs.475506.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3QWMX-ray2.39A743-880[»]
4C0AX-ray3.30A/B/E/F512-885[»]
ProteinModelPortalQ6DN90.
SMRQ6DN90. Positions 515-880.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115250. 7 interactions.
IntActQ6DN90. 6 interactions.
STRING9606.ENSP00000273221.

Polymorphism databases

DMDM74748429.

Proteomic databases

MaxQBQ6DN90.
PaxDbQ6DN90.
PRIDEQ6DN90.

Protocols and materials databases

DNASU9922.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000273221; ENSP00000273221; ENSG00000144711. [Q6DN90-1]
GeneID9922.
KEGGhsa:9922.
UCSCuc003bxt.3. human. [Q6DN90-1]

Organism-specific databases

CTD9922.
GeneCardsGC03M012938.
HGNCHGNC:29112. IQSEC1.
HPAHPA038143.
HPA038144.
MIM610166. gene.
neXtProtNX_Q6DN90.
PharmGKBPA128394566.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5307.
HOGENOMHOG000113099.
HOVERGENHBG056324.
InParanoidQ6DN90.
KOK12495.
PhylomeDBQ6DN90.
TreeFamTF323811.

Gene expression databases

ArrayExpressQ6DN90.
BgeeQ6DN90.
CleanExHS_IQSEC1.
GenevestigatorQ6DN90.

Family and domain databases

Gene3D1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProIPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamPF01369. Sec7. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMSSF48425. SSF48425. 1 hit.
PROSITEPS50096. IQ. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIQSEC1. human.
GeneWikiIQSEC1.
GenomeRNAi9922.
NextBio37432.
PROQ6DN90.
SOURCESearch...

Entry information

Entry nameIQEC1_HUMAN
AccessionPrimary (citable) accession number: Q6DN90
Secondary accession number(s): O94863, Q96D85
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM