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Protein

IQ motif and SEC7 domain-containing protein 1

Gene

IQSEC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor for ARF1 and ARF6 (PubMed:24058294). Guanine nucleotide exchange factor activity is enhanced by lipid binding (PubMed:24058294). Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
IQ motif and SEC7 domain-containing protein 1
Alternative name(s):
ADP-ribosylation factors guanine nucleotide-exchange protein 100
ADP-ribosylation factors guanine nucleotide-exchange protein 2
Brefeldin-resistant Arf-GEF 2 protein1 Publication
Short name:
BRAG21 Publication
Gene namesi
Name:IQSEC1
Synonyms:ARFGEP100, BRAG2, KIAA0763
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:29112. IQSEC1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi620E → K: Abolishes guanosine nucleotide exchange factor activity. 1 Publication1

Organism-specific databases

DisGeNETi9922.
OpenTargetsiENSG00000144711.
PharmGKBiPA128394566.

Polymorphism and mutation databases

BioMutaiIQSEC1.
DMDMi74748429.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002456061 – 963IQ motif and SEC7 domain-containing protein 1Add BLAST963

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei89PhosphoserineCombined sources1
Modified residuei105PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei180PhosphoserineCombined sources1
Modified residuei249PhosphoserineBy similarity1
Modified residuei253PhosphoserineCombined sources1
Modified residuei512PhosphoserineCombined sources1
Modified residuei515PhosphoserineCombined sources1
Modified residuei892PhosphoserineCombined sources1
Modified residuei911PhosphotyrosineCombined sources1
Modified residuei924PhosphoserineCombined sources1
Modified residuei925PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6DN90.
MaxQBiQ6DN90.
PaxDbiQ6DN90.
PeptideAtlasiQ6DN90.
PRIDEiQ6DN90.

PTM databases

iPTMnetiQ6DN90.
PhosphoSitePlusiQ6DN90.

Expressioni

Tissue specificityi

Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.2 Publications

Gene expression databases

BgeeiENSG00000144711.
CleanExiHS_IQSEC1.
ExpressionAtlasiQ6DN90. baseline and differential.
GenevisibleiQ6DN90. HS.

Organism-specific databases

HPAiHPA038143.
HPA038144.

Interactioni

Subunit structurei

Interacts with ARF1 and ARF6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GNAQP501482EBI-3044091,EBI-3909604

Protein-protein interaction databases

BioGridi115250. 18 interactors.
IntActiQ6DN90. 12 interactors.
STRINGi9606.ENSP00000273221.

Structurei

Secondary structure

1963
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi522 – 537Combined sources16
Helixi539 – 548Combined sources10
Helixi556 – 565Combined sources10
Helixi571 – 578Combined sources8
Helixi584 – 595Combined sources12
Helixi604 – 614Combined sources11
Helixi621 – 638Combined sources18
Helixi640 – 646Combined sources7
Helixi650 – 667Combined sources18
Turni669 – 671Combined sources3
Beta strandi673 – 676Combined sources4
Helixi679 – 685Combined sources7
Turni686 – 689Combined sources4
Helixi697 – 709Combined sources13
Helixi718 – 728Combined sources11
Beta strandi753 – 761Combined sources9
Turni771 – 774Combined sources4
Beta strandi775 – 783Combined sources9
Beta strandi785 – 789Combined sources5
Beta strandi803 – 806Combined sources4
Beta strandi811 – 816Combined sources6
Beta strandi824 – 829Combined sources6
Beta strandi832 – 834Combined sources3
Beta strandi837 – 843Combined sources7
Helixi847 – 878Combined sources32

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QWMX-ray2.39A743-880[»]
4C0AX-ray3.30A/B/E/F512-885[»]
ProteinModelPortaliQ6DN90.
SMRiQ6DN90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 163IQPROSITE-ProRule annotationAdd BLAST30
Domaini517 – 710SEC7PROSITE-ProRule annotationAdd BLAST194
Domaini774 – 866PHAdd BLAST93

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili848 – 879Sequence analysisAdd BLAST32

Domaini

The PH domain mediates interaction with lipid membranes that contain phosphatidylinositol-4,5-bisphosphate, but does not bind membranes that lack phosphatidylinositol-4,5-bisphosphate.1 Publication

Sequence similaritiesi

Belongs to the BRAG family.Curated
Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 PH domain.Curated
Contains 1 SEC7 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0931. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000113099.
HOVERGENiHBG056324.
InParanoidiQ6DN90.
KOiK12495.
PhylomeDBiQ6DN90.
TreeFamiTF323811.

Family and domain databases

CDDicd13318. PH_IQSEC. 1 hit.
Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR030737. IQSEC1.
IPR033742. IQSEC_PH.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PANTHERiPTHR10663:SF63. PTHR10663:SF63. 1 hit.
PfamiPF16453. IQ_SEC7_PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6DN90-1) [UniParc]FASTAAdd to basket
Also known as: BRAG2b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWCLHCNSER TQSLLELELD SGVEGEAPSS ETGTSLDSPS AYPQGPLVPG
60 70 80 90 100
SSLSPDHYEH TSVGAYGLYS GPPGQQQRTR RPKLQHSTSI LRKQAEEEAI
110 120 130 140 150
KRSRSLSESY ELSSDLQDKQ VEMLERKYGG RLVTRHAART IQTAFRQYQM
160 170 180 190 200
NKNFERLRSS MSENRMSRRI VLSNMRMQFS FEGPEKVHSS YFEGKQVSVT
210 220 230 240 250
NDGSQLGALV SPECGDLSEP TTLKSPAPSS DFADAITELE DAFSRQVKSL
260 270 280 290 300
AESIDDALNC RSLHTEEAPA LDAARARDTE PQTALHGMDH RKLDEMTASY
310 320 330 340 350
SDVTLYIDEE ELSPPLPLSQ AGDRPSSTES DLRLRAGGAA PDYWALAHKE
360 370 380 390 400
DKADTDTSCR STPSLERQEQ RLRVEHLPLL TIEPPSDSSV DLSDRSERGS
410 420 430 440 450
LKRQSAYERS LGGQQGSPKH GPHSGAPKSL PREEPELRPR PPRPLDSHLA
460 470 480 490 500
INGSANRQSK SESDYSDGDN DSINSTSNSN DTINCSSESS SRDSLREQTL
510 520 530 540 550
SKQTYHKEAR NSWDSPAFSN DVIRKRHYRI GLNLFNKKPE KGVQYLIERG
560 570 580 590 600
FVPDTPVGVA HFLLQRKGLS RQMIGEFLGN RQKQFNRDVL DCVVDEMDFS
610 620 630 640 650
TMELDEALRK FQAHIRVQGE AQKVERLIEA FSQRYCICNP GVVRQFRNPD
660 670 680 690 700
TIFILAFAII LLNTDMYSPN VKPERKMKLE DFIKNLRGVD DGEDIPREML
710 720 730 740 750
MGIYERIRKR ELKTNEDHVS QVQKVEKLIV GKKPIGSLHP GLGCVLSLPH
760 770 780 790 800
RRLVCYCRLF EVPDPNKPQK LGLHQREIFL FNDLLVVTKI FQKKKNSVTY
810 820 830 840 850
SFRQSFSLYG MQVLLFENQY YPNGIRLTSS VPGADIKVLI NFNAPNPQDR
860 870 880 890 900
KKFTDDLRES IAEVQEMEKH RIESELEKQK GVVRPSMSQC SSLKKESGNG
910 920 930 940 950
TLSRACLDDS YASGEGLKRS ALSSSLRDLS EAGKRGRRSS AGSLESNVEF
960
QPFEPLQPSV LCS
Length:963
Mass (Da):108,314
Last modified:August 16, 2004 - v1
Checksum:i5B31F9918F9CFF11
GO
Isoform 2 (identifier: Q6DN90-2) [UniParc]FASTAAdd to basket
Also known as: BRAG2a

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.

Note: No experimental confirmation available.
Show »
Length:841
Mass (Da):94,914
Checksum:i504A8DDC71EB8B71
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti934 – 936KRG → VHH (PubMed:15489334).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051927640P → S.Corresponds to variant rs35319679dbSNPEnsembl.1
Natural variantiVAR_027004882V → I.Corresponds to variant rs17541405dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0197581 – 122Missing in isoform 2. 2 PublicationsAdd BLAST122

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653734 mRNA. Translation: AAT72063.1.
AB018306 mRNA. Translation: BAA34483.2.
BC010267 mRNA. Translation: AAH10267.1.
CCDSiCCDS33703.1. [Q6DN90-1]
RefSeqiNP_001127854.1. NM_001134382.2.
NP_001317548.1. NM_001330619.1.
NP_055684.3. NM_014869.6. [Q6DN90-1]
XP_011532617.1. XM_011534315.2.
UniGeneiHs.475506.

Genome annotation databases

EnsembliENST00000273221; ENSP00000273221; ENSG00000144711. [Q6DN90-1]
GeneIDi9922.
KEGGihsa:9922.
UCSCiuc003bxt.4. human. [Q6DN90-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653734 mRNA. Translation: AAT72063.1.
AB018306 mRNA. Translation: BAA34483.2.
BC010267 mRNA. Translation: AAH10267.1.
CCDSiCCDS33703.1. [Q6DN90-1]
RefSeqiNP_001127854.1. NM_001134382.2.
NP_001317548.1. NM_001330619.1.
NP_055684.3. NM_014869.6. [Q6DN90-1]
XP_011532617.1. XM_011534315.2.
UniGeneiHs.475506.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QWMX-ray2.39A743-880[»]
4C0AX-ray3.30A/B/E/F512-885[»]
ProteinModelPortaliQ6DN90.
SMRiQ6DN90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115250. 18 interactors.
IntActiQ6DN90. 12 interactors.
STRINGi9606.ENSP00000273221.

PTM databases

iPTMnetiQ6DN90.
PhosphoSitePlusiQ6DN90.

Polymorphism and mutation databases

BioMutaiIQSEC1.
DMDMi74748429.

Proteomic databases

EPDiQ6DN90.
MaxQBiQ6DN90.
PaxDbiQ6DN90.
PeptideAtlasiQ6DN90.
PRIDEiQ6DN90.

Protocols and materials databases

DNASUi9922.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000273221; ENSP00000273221; ENSG00000144711. [Q6DN90-1]
GeneIDi9922.
KEGGihsa:9922.
UCSCiuc003bxt.4. human. [Q6DN90-1]

Organism-specific databases

CTDi9922.
DisGeNETi9922.
GeneCardsiIQSEC1.
HGNCiHGNC:29112. IQSEC1.
HPAiHPA038143.
HPA038144.
MIMi610166. gene.
neXtProtiNX_Q6DN90.
OpenTargetsiENSG00000144711.
PharmGKBiPA128394566.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0931. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000113099.
HOVERGENiHBG056324.
InParanoidiQ6DN90.
KOiK12495.
PhylomeDBiQ6DN90.
TreeFamiTF323811.

Miscellaneous databases

ChiTaRSiIQSEC1. human.
GeneWikiiIQSEC1.
GenomeRNAii9922.
PROiQ6DN90.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000144711.
CleanExiHS_IQSEC1.
ExpressionAtlasiQ6DN90. baseline and differential.
GenevisibleiQ6DN90. HS.

Family and domain databases

CDDicd13318. PH_IQSEC. 1 hit.
Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR030737. IQSEC1.
IPR033742. IQSEC_PH.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PANTHERiPTHR10663:SF63. PTHR10663:SF63. 1 hit.
PfamiPF16453. IQ_SEC7_PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIQEC1_HUMAN
AccessioniPrimary (citable) accession number: Q6DN90
Secondary accession number(s): O94863, Q96D85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: August 16, 2004
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.