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Protein

IQ motif and SEC7 domain-containing protein 1

Gene

IQSEC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor for ARF1 and ARF6 (PubMed:24058294). Guanine nucleotide exchange factor activity is enhanced by lipid binding (PubMed:24058294). Accelerates GTP binding by ARFs of all three classes. Guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
IQ motif and SEC7 domain-containing protein 1
Alternative name(s):
ADP-ribosylation factors guanine nucleotide-exchange protein 100
ADP-ribosylation factors guanine nucleotide-exchange protein 2
Brefeldin-resistant Arf-GEF 2 protein1 Publication
Short name:
BRAG21 Publication
Gene namesi
Name:IQSEC1
Synonyms:ARFGEP100, BRAG2, KIAA0763
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000144711.13
HGNCiHGNC:29112 IQSEC1
MIMi610166 gene
neXtProtiNX_Q6DN90

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi620E → K: Abolishes guanosine nucleotide exchange factor activity. 1 Publication1

Organism-specific databases

DisGeNETi9922
OpenTargetsiENSG00000144711
PharmGKBiPA128394566

Polymorphism and mutation databases

BioMutaiIQSEC1
DMDMi74748429

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002456061 – 963IQ motif and SEC7 domain-containing protein 1Add BLAST963

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei89PhosphoserineCombined sources1
Modified residuei105PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei180PhosphoserineCombined sources1
Modified residuei249PhosphoserineBy similarity1
Modified residuei253PhosphoserineCombined sources1
Modified residuei512PhosphoserineCombined sources1
Modified residuei515PhosphoserineCombined sources1
Modified residuei892PhosphoserineCombined sources1
Modified residuei911PhosphotyrosineCombined sources1
Modified residuei924PhosphoserineCombined sources1
Modified residuei925PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6DN90
PaxDbiQ6DN90
PeptideAtlasiQ6DN90
PRIDEiQ6DN90

PTM databases

iPTMnetiQ6DN90
PhosphoSitePlusiQ6DN90

Expressioni

Tissue specificityi

Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.2 Publications

Gene expression databases

BgeeiENSG00000144711
CleanExiHS_IQSEC1
ExpressionAtlasiQ6DN90 baseline and differential
GenevisibleiQ6DN90 HS

Organism-specific databases

HPAiHPA038143
HPA038144

Interactioni

Subunit structurei

Interacts with ARF1 and ARF6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GNAQP501482EBI-3044091,EBI-3909604

GO - Molecular functioni

Protein-protein interaction databases

BioGridi115250, 29 interactors
IntActiQ6DN90, 20 interactors
MINTiQ6DN90
STRINGi9606.ENSP00000273221

Structurei

Secondary structure

1963
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi516 – 518Combined sources3
Helixi522 – 537Combined sources16
Helixi539 – 548Combined sources10
Helixi556 – 565Combined sources10
Helixi571 – 578Combined sources8
Helixi584 – 594Combined sources11
Helixi604 – 614Combined sources11
Helixi621 – 638Combined sources18
Helixi640 – 643Combined sources4
Beta strandi646 – 648Combined sources3
Helixi651 – 666Combined sources16
Turni669 – 671Combined sources3
Helixi673 – 675Combined sources3
Helixi679 – 685Combined sources7
Turni686 – 689Combined sources4
Helixi697 – 709Combined sources13
Helixi718 – 728Combined sources11
Beta strandi729 – 731Combined sources3
Beta strandi753 – 761Combined sources9
Beta strandi774 – 781Combined sources8
Beta strandi784 – 793Combined sources10
Beta strandi798 – 807Combined sources10
Beta strandi811 – 816Combined sources6
Beta strandi824 – 829Combined sources6
Beta strandi832 – 834Combined sources3
Beta strandi837 – 843Combined sources7
Helixi847 – 871Combined sources25
Helixi874 – 877Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QWMX-ray2.39A743-880[»]
4C0AX-ray3.30A/B/E/F512-885[»]
5NLVX-ray2.40A512-885[»]
5NLYX-ray2.00A/B512-885[»]
ProteinModelPortaliQ6DN90
SMRiQ6DN90
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 163IQPROSITE-ProRule annotationAdd BLAST30
Domaini517 – 710SEC7PROSITE-ProRule annotationAdd BLAST194
Domaini774 – 866PHAdd BLAST93

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili848 – 879Sequence analysisAdd BLAST32

Domaini

The PH domain mediates interaction with lipid membranes that contain phosphatidylinositol-4,5-bisphosphate, but does not bind membranes that lack phosphatidylinositol-4,5-bisphosphate.1 Publication

Sequence similaritiesi

Belongs to the BRAG family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0931 Eukaryota
COG5307 LUCA
GeneTreeiENSGT00760000119036
HOGENOMiHOG000113099
HOVERGENiHBG056324
InParanoidiQ6DN90
KOiK12495
PhylomeDBiQ6DN90
TreeFamiTF323811

Family and domain databases

CDDicd13318 PH_IQSEC, 1 hit
Gene3Di1.10.1000.11, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR030737 IQSEC1
IPR033742 IQSEC_PH
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR023394 Sec7_C_sf
IPR000904 Sec7_dom
IPR035999 Sec7_dom_sf
PANTHERiPTHR44233:SF3 PTHR44233:SF3, 1 hit
PfamiView protein in Pfam
PF16453 IQ_SEC7_PH, 1 hit
PF01369 Sec7, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00222 Sec7, 1 hit
SUPFAMiSSF48425 SSF48425, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 1 hit
PS50190 SEC7, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6DN90-1) [UniParc]FASTAAdd to basket
Also known as: BRAG2b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWCLHCNSER TQSLLELELD SGVEGEAPSS ETGTSLDSPS AYPQGPLVPG
60 70 80 90 100
SSLSPDHYEH TSVGAYGLYS GPPGQQQRTR RPKLQHSTSI LRKQAEEEAI
110 120 130 140 150
KRSRSLSESY ELSSDLQDKQ VEMLERKYGG RLVTRHAART IQTAFRQYQM
160 170 180 190 200
NKNFERLRSS MSENRMSRRI VLSNMRMQFS FEGPEKVHSS YFEGKQVSVT
210 220 230 240 250
NDGSQLGALV SPECGDLSEP TTLKSPAPSS DFADAITELE DAFSRQVKSL
260 270 280 290 300
AESIDDALNC RSLHTEEAPA LDAARARDTE PQTALHGMDH RKLDEMTASY
310 320 330 340 350
SDVTLYIDEE ELSPPLPLSQ AGDRPSSTES DLRLRAGGAA PDYWALAHKE
360 370 380 390 400
DKADTDTSCR STPSLERQEQ RLRVEHLPLL TIEPPSDSSV DLSDRSERGS
410 420 430 440 450
LKRQSAYERS LGGQQGSPKH GPHSGAPKSL PREEPELRPR PPRPLDSHLA
460 470 480 490 500
INGSANRQSK SESDYSDGDN DSINSTSNSN DTINCSSESS SRDSLREQTL
510 520 530 540 550
SKQTYHKEAR NSWDSPAFSN DVIRKRHYRI GLNLFNKKPE KGVQYLIERG
560 570 580 590 600
FVPDTPVGVA HFLLQRKGLS RQMIGEFLGN RQKQFNRDVL DCVVDEMDFS
610 620 630 640 650
TMELDEALRK FQAHIRVQGE AQKVERLIEA FSQRYCICNP GVVRQFRNPD
660 670 680 690 700
TIFILAFAII LLNTDMYSPN VKPERKMKLE DFIKNLRGVD DGEDIPREML
710 720 730 740 750
MGIYERIRKR ELKTNEDHVS QVQKVEKLIV GKKPIGSLHP GLGCVLSLPH
760 770 780 790 800
RRLVCYCRLF EVPDPNKPQK LGLHQREIFL FNDLLVVTKI FQKKKNSVTY
810 820 830 840 850
SFRQSFSLYG MQVLLFENQY YPNGIRLTSS VPGADIKVLI NFNAPNPQDR
860 870 880 890 900
KKFTDDLRES IAEVQEMEKH RIESELEKQK GVVRPSMSQC SSLKKESGNG
910 920 930 940 950
TLSRACLDDS YASGEGLKRS ALSSSLRDLS EAGKRGRRSS AGSLESNVEF
960
QPFEPLQPSV LCS
Length:963
Mass (Da):108,314
Last modified:August 16, 2004 - v1
Checksum:i5B31F9918F9CFF11
GO
Isoform 2 (identifier: Q6DN90-2) [UniParc]FASTAAdd to basket
Also known as: BRAG2a

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.

Note: No experimental confirmation available.
Show »
Length:841
Mass (Da):94,914
Checksum:i504A8DDC71EB8B71
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti934 – 936KRG → VHH (PubMed:15489334).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051927640P → S. Corresponds to variant dbSNP:rs35319679Ensembl.1
Natural variantiVAR_027004882V → I. Corresponds to variant dbSNP:rs17541405Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0197581 – 122Missing in isoform 2. 2 PublicationsAdd BLAST122

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY653734 mRNA Translation: AAT72063.1
AB018306 mRNA Translation: BAA34483.2
BC010267 mRNA Translation: AAH10267.1
CCDSiCCDS33703.1 [Q6DN90-1]
RefSeqiNP_001127854.1, NM_001134382.2
NP_001317548.1, NM_001330619.1
NP_055684.3, NM_014869.6 [Q6DN90-1]
XP_011532617.1, XM_011534315.2
UniGeneiHs.475506

Genome annotation databases

EnsembliENST00000273221; ENSP00000273221; ENSG00000144711 [Q6DN90-1]
GeneIDi9922
KEGGihsa:9922
UCSCiuc003bxt.4 human [Q6DN90-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiIQEC1_HUMAN
AccessioniPrimary (citable) accession number: Q6DN90
Secondary accession number(s): O94863, Q96D85
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: August 16, 2004
Last modified: April 25, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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