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Q6DN90

- IQEC1_HUMAN

UniProt

Q6DN90 - IQEC1_HUMAN

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Protein

IQ motif and SEC7 domain-containing protein 1

Gene
IQSEC1, ARFGEP100, BRAG2, KIAA0763
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin.2 Publications

GO - Molecular functioni

  1. ARF guanyl-nucleotide exchange factor activity Source: RefGenome

GO - Biological processi

  1. actin cytoskeleton organization Source: RefGenome
  2. regulation of ARF protein signal transduction Source: InterPro
  3. regulation of GTPase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
IQ motif and SEC7 domain-containing protein 1
Alternative name(s):
ADP-ribosylation factors guanine nucleotide-exchange protein 100
ADP-ribosylation factors guanine nucleotide-exchange protein 2
Brefeldin-resistant Arf-GEF 2 protein
Gene namesi
Name:IQSEC1
Synonyms:ARFGEP100, BRAG2, KIAA0763
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:29112. IQSEC1.

Subcellular locationi

Cytoplasm. Nucleus
Note: At steady state, may be preferentially cytosolic.1 Publication

GO - Cellular componenti

  1. cell junction Source: HPA
  2. cytoplasm Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: HPA
  4. nucleolus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA128394566.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 963963IQ motif and SEC7 domain-containing protein 1PRO_0000245606Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei180 – 1801Phosphoserine1 Publication
Modified residuei512 – 5121Phosphoserine2 Publications
Modified residuei515 – 5151Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6DN90.
PaxDbiQ6DN90.
PRIDEiQ6DN90.

Expressioni

Tissue specificityi

Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis.2 Publications

Gene expression databases

ArrayExpressiQ6DN90.
BgeeiQ6DN90.
CleanExiHS_IQSEC1.
GenevestigatoriQ6DN90.

Organism-specific databases

HPAiHPA038143.
HPA038144.

Interactioni

Subunit structurei

Interacts with ARF6.1 Publication

Protein-protein interaction databases

BioGridi115250. 8 interactions.
IntActiQ6DN90. 6 interactions.
STRINGi9606.ENSP00000273221.

Structurei

Secondary structure

1
963
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi522 – 53716
Helixi539 – 54810
Helixi556 – 56510
Helixi571 – 5788
Helixi584 – 59512
Helixi604 – 61411
Helixi621 – 63818
Helixi640 – 6467
Helixi650 – 66718
Turni669 – 6713
Beta strandi673 – 6764
Helixi679 – 6857
Turni686 – 6894
Helixi697 – 70913
Helixi718 – 72811
Beta strandi753 – 7619
Turni771 – 7744
Beta strandi775 – 7839
Beta strandi785 – 7895
Beta strandi803 – 8064
Beta strandi811 – 8166
Beta strandi824 – 8296
Beta strandi832 – 8343
Beta strandi837 – 8437
Helixi847 – 87832

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QWMX-ray2.39A743-880[»]
4C0AX-ray3.30A/B/E/F512-885[»]
ProteinModelPortaliQ6DN90.
SMRiQ6DN90. Positions 515-880.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 16330IQAdd
BLAST
Domaini517 – 710194SEC7Add
BLAST
Domaini774 – 86693PHAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili848 – 87932 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the BRAG family.
Contains 1 IQ domain.
Contains 1 PH domain.
Contains 1 SEC7 domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5307.
HOGENOMiHOG000113099.
HOVERGENiHBG056324.
InParanoidiQ6DN90.
KOiK12495.
PhylomeDBiQ6DN90.
TreeFamiTF323811.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6DN90-1) [UniParc]FASTAAdd to Basket

Also known as: BRAG2b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MWCLHCNSER TQSLLELELD SGVEGEAPSS ETGTSLDSPS AYPQGPLVPG    50
SSLSPDHYEH TSVGAYGLYS GPPGQQQRTR RPKLQHSTSI LRKQAEEEAI 100
KRSRSLSESY ELSSDLQDKQ VEMLERKYGG RLVTRHAART IQTAFRQYQM 150
NKNFERLRSS MSENRMSRRI VLSNMRMQFS FEGPEKVHSS YFEGKQVSVT 200
NDGSQLGALV SPECGDLSEP TTLKSPAPSS DFADAITELE DAFSRQVKSL 250
AESIDDALNC RSLHTEEAPA LDAARARDTE PQTALHGMDH RKLDEMTASY 300
SDVTLYIDEE ELSPPLPLSQ AGDRPSSTES DLRLRAGGAA PDYWALAHKE 350
DKADTDTSCR STPSLERQEQ RLRVEHLPLL TIEPPSDSSV DLSDRSERGS 400
LKRQSAYERS LGGQQGSPKH GPHSGAPKSL PREEPELRPR PPRPLDSHLA 450
INGSANRQSK SESDYSDGDN DSINSTSNSN DTINCSSESS SRDSLREQTL 500
SKQTYHKEAR NSWDSPAFSN DVIRKRHYRI GLNLFNKKPE KGVQYLIERG 550
FVPDTPVGVA HFLLQRKGLS RQMIGEFLGN RQKQFNRDVL DCVVDEMDFS 600
TMELDEALRK FQAHIRVQGE AQKVERLIEA FSQRYCICNP GVVRQFRNPD 650
TIFILAFAII LLNTDMYSPN VKPERKMKLE DFIKNLRGVD DGEDIPREML 700
MGIYERIRKR ELKTNEDHVS QVQKVEKLIV GKKPIGSLHP GLGCVLSLPH 750
RRLVCYCRLF EVPDPNKPQK LGLHQREIFL FNDLLVVTKI FQKKKNSVTY 800
SFRQSFSLYG MQVLLFENQY YPNGIRLTSS VPGADIKVLI NFNAPNPQDR 850
KKFTDDLRES IAEVQEMEKH RIESELEKQK GVVRPSMSQC SSLKKESGNG 900
TLSRACLDDS YASGEGLKRS ALSSSLRDLS EAGKRGRRSS AGSLESNVEF 950
QPFEPLQPSV LCS 963
Length:963
Mass (Da):108,314
Last modified:August 16, 2004 - v1
Checksum:i5B31F9918F9CFF11
GO
Isoform 2 (identifier: Q6DN90-2) [UniParc]FASTAAdd to Basket

Also known as: BRAG2a

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.

Note: No experimental confirmation available.

Show »
Length:841
Mass (Da):94,914
Checksum:i504A8DDC71EB8B71
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti640 – 6401P → S.
Corresponds to variant rs35319679 [ dbSNP | Ensembl ].
VAR_051927
Natural varianti882 – 8821V → I.
Corresponds to variant rs17541405 [ dbSNP | Ensembl ].
VAR_027004

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 122122Missing in isoform 2. VSP_019758Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti934 – 9363KRG → VHH1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653734 mRNA. Translation: AAT72063.1.
AB018306 mRNA. Translation: BAA34483.2.
BC010267 mRNA. Translation: AAH10267.1.
CCDSiCCDS33703.1. [Q6DN90-1]
RefSeqiNP_001127854.1. NM_001134382.2.
NP_055684.3. NM_014869.6. [Q6DN90-1]
UniGeneiHs.475506.

Genome annotation databases

EnsembliENST00000273221; ENSP00000273221; ENSG00000144711. [Q6DN90-1]
GeneIDi9922.
KEGGihsa:9922.
UCSCiuc003bxt.3. human. [Q6DN90-1]

Polymorphism databases

DMDMi74748429.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY653734 mRNA. Translation: AAT72063.1 .
AB018306 mRNA. Translation: BAA34483.2 .
BC010267 mRNA. Translation: AAH10267.1 .
CCDSi CCDS33703.1. [Q6DN90-1 ]
RefSeqi NP_001127854.1. NM_001134382.2.
NP_055684.3. NM_014869.6. [Q6DN90-1 ]
UniGenei Hs.475506.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3QWM X-ray 2.39 A 743-880 [» ]
4C0A X-ray 3.30 A/B/E/F 512-885 [» ]
ProteinModelPortali Q6DN90.
SMRi Q6DN90. Positions 515-880.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115250. 8 interactions.
IntActi Q6DN90. 6 interactions.
STRINGi 9606.ENSP00000273221.

Polymorphism databases

DMDMi 74748429.

Proteomic databases

MaxQBi Q6DN90.
PaxDbi Q6DN90.
PRIDEi Q6DN90.

Protocols and materials databases

DNASUi 9922.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000273221 ; ENSP00000273221 ; ENSG00000144711 . [Q6DN90-1 ]
GeneIDi 9922.
KEGGi hsa:9922.
UCSCi uc003bxt.3. human. [Q6DN90-1 ]

Organism-specific databases

CTDi 9922.
GeneCardsi GC03M012938.
HGNCi HGNC:29112. IQSEC1.
HPAi HPA038143.
HPA038144.
MIMi 610166. gene.
neXtProti NX_Q6DN90.
PharmGKBi PA128394566.
HUGEi Search...
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5307.
HOGENOMi HOG000113099.
HOVERGENi HBG056324.
InParanoidi Q6DN90.
KOi K12495.
PhylomeDBi Q6DN90.
TreeFami TF323811.

Miscellaneous databases

ChiTaRSi IQSEC1. human.
GeneWikii IQSEC1.
GenomeRNAii 9922.
NextBioi 37432.
PROi Q6DN90.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6DN90.
Bgeei Q6DN90.
CleanExi HS_IQSEC1.
Genevestigatori Q6DN90.

Family and domain databases

Gene3Di 1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR000048. IQ_motif_EF-hand-BS.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view ]
Pfami PF01369. Sec7. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view ]
SUPFAMi SSF48425. SSF48425. 1 hit.
PROSITEi PS50096. IQ. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins."
    Dunphy J.L., Moravec R., Ly K., Lasell T.K., Melancon P., Casanova J.E.
    Curr. Biol. 16:315-320(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
  2. "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-936 (ISOFORM 2).
    Tissue: Placenta.
  4. "ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6."
    Someya A., Sata M., Takeda K., Pacheco-Rodriguez G., Ferrans V.J., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 98:2413-2418(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ARF6.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-512, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180 AND SER-512, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIQEC1_HUMAN
AccessioniPrimary (citable) accession number: Q6DN90
Secondary accession number(s): O94863, Q96D85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: August 16, 2004
Last modified: September 3, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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