Reviewed, UniProtKB/Swiss-Prot Q6DN90 (IQEC1_HUMAN)

Names and origin

Protein names

Recommended name:
    IQ motif and SEC7 domain-containing protein 1
Alternative name(s):
    ADP-ribosylation factors guanine nucleotide-exchange protein 100
    ADP-ribosylation factors guanine nucleotide-exchange protein 2
    Brefeldin-resistant Arf-GEF 2 protein

Gene names

Name:	IQSEC1
Synonyms:	ARFGEP100, BRAG2, KIAA0763

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	963 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin. Ref.1 Ref.4
Subunit structure	Interacts with ARF6. Ref.4
Subcellular location	Cytoplasm. Nucleus. Note: At steady state, may be preferentially cytosolic. Ref.4
Tissue specificity	Expressed in brain, ovary, heart, lung, liver, kidney and leukocytes. Moderate expression was also detected in lung, skeletal muscle, placenta, small intestine, pancreas, spleen and testis. Ref.4 Ref.2
Sequence similarities	Belongs to the BRAG family. Contains 1 IQ domain. Contains 1 PH domain. Contains 1 SEC7 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ARF guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6DN90-1)

Also known as: BRAG2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6DN90-2)

Also known as: BRAG2a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-122: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 963	963	IQ motif and SEC7 domain-containing protein 1		PRO_0000245606
Regions
	Domain	134 – 163	30	IQ
	Domain	517 – 710	194	SEC7
	Domain	774 – 866	93	PH
	Coiled coil	848 – 879	32	Potential
Amino acid modifications
	Modified residue	107	1	Phosphoserine By similarity
	Modified residue	180	1	Phosphoserine Ref.6
	Modified residue	362	1	Phosphothreonine Ref.5
	Modified residue	512	1	Phosphoserine Ref.6
	Modified residue	515	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 122	122	Missing in isoform 2.		VSP_019758
	Natural variant	640	1	P → S: dbSNP rs35319679.		VAR_051927
	Natural variant	882	1	V → I: dbSNP rs17541405.		VAR_027004
Experimental info
	Sequence conflict	934 – 936	3	KRG → VHH Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (BRAG2b) [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 5B31F9918F9CFF11	FASTA	963	108,314
	10 20 30 40 50 60 MWCLHCNSER TQSLLELELD SGVEGEAPSS ETGTSLDSPS AYPQGPLVPG SSLSPDHYEH 70 80 90 100 110 120 TSVGAYGLYS GPPGQQQRTR RPKLQHSTSI LRKQAEEEAI KRSRSLSESY ELSSDLQDKQ 130 140 150 160 170 180 VEMLERKYGG RLVTRHAART IQTAFRQYQM NKNFERLRSS MSENRMSRRI VLSNMRMQFS 190 200 210 220 230 240 FEGPEKVHSS YFEGKQVSVT NDGSQLGALV SPECGDLSEP TTLKSPAPSS DFADAITELE 250 260 270 280 290 300 DAFSRQVKSL AESIDDALNC RSLHTEEAPA LDAARARDTE PQTALHGMDH RKLDEMTASY 310 320 330 340 350 360 SDVTLYIDEE ELSPPLPLSQ AGDRPSSTES DLRLRAGGAA PDYWALAHKE DKADTDTSCR 370 380 390 400 410 420 STPSLERQEQ RLRVEHLPLL TIEPPSDSSV DLSDRSERGS LKRQSAYERS LGGQQGSPKH 430 440 450 460 470 480 GPHSGAPKSL PREEPELRPR PPRPLDSHLA INGSANRQSK SESDYSDGDN DSINSTSNSN 490 500 510 520 530 540 DTINCSSESS SRDSLREQTL SKQTYHKEAR NSWDSPAFSN DVIRKRHYRI GLNLFNKKPE 550 560 570 580 590 600 KGVQYLIERG FVPDTPVGVA HFLLQRKGLS RQMIGEFLGN RQKQFNRDVL DCVVDEMDFS 610 620 630 640 650 660 TMELDEALRK FQAHIRVQGE AQKVERLIEA FSQRYCICNP GVVRQFRNPD TIFILAFAII 670 680 690 700 710 720 LLNTDMYSPN VKPERKMKLE DFIKNLRGVD DGEDIPREML MGIYERIRKR ELKTNEDHVS 730 740 750 760 770 780 QVQKVEKLIV GKKPIGSLHP GLGCVLSLPH RRLVCYCRLF EVPDPNKPQK LGLHQREIFL 790 800 810 820 830 840 FNDLLVVTKI FQKKKNSVTY SFRQSFSLYG MQVLLFENQY YPNGIRLTSS VPGADIKVLI 850 860 870 880 890 900 NFNAPNPQDR KKFTDDLRES IAEVQEMEKH RIESELEKQK GVVRPSMSQC SSLKKESGNG 910 920 930 940 950 960 TLSRACLDDS YASGEGLKRS ALSSSLRDLS EAGKRGRRSS AGSLESNVEF QPFEPLQPSV LCS
	Isoform 2 (BRAG2a). Checksum: 504A8DDC71EB8B71	FASTA	841	94,914
	10 20 30 40 50 60 MLERKYGGRL VTRHAARTIQ TAFRQYQMNK NFERLRSSMS ENRMSRRIVL SNMRMQFSFE 70 80 90 100 110 120 GPEKVHSSYF EGKQVSVTND GSQLGALVSP ECGDLSEPTT LKSPAPSSDF ADAITELEDA 130 140 150 160 170 180 FSRQVKSLAE SIDDALNCRS LHTEEAPALD AARARDTEPQ TALHGMDHRK LDEMTASYSD 190 200 210 220 230 240 VTLYIDEEEL SPPLPLSQAG DRPSSTESDL RLRAGGAAPD YWALAHKEDK ADTDTSCRST 250 260 270 280 290 300 PSLERQEQRL RVEHLPLLTI EPPSDSSVDL SDRSERGSLK RQSAYERSLG GQQGSPKHGP 310 320 330 340 350 360 HSGAPKSLPR EEPELRPRPP RPLDSHLAIN GSANRQSKSE SDYSDGDNDS INSTSNSNDT 370 380 390 400 410 420 INCSSESSSR DSLREQTLSK QTYHKEARNS WDSPAFSNDV IRKRHYRIGL NLFNKKPEKG 430 440 450 460 470 480 VQYLIERGFV PDTPVGVAHF LLQRKGLSRQ MIGEFLGNRQ KQFNRDVLDC VVDEMDFSTM 490 500 510 520 530 540 ELDEALRKFQ AHIRVQGEAQ KVERLIEAFS QRYCICNPGV VRQFRNPDTI FILAFAIILL 550 560 570 580 590 600 NTDMYSPNVK PERKMKLEDF IKNLRGVDDG EDIPREMLMG IYERIRKREL KTNEDHVSQV 610 620 630 640 650 660 QKVEKLIVGK KPIGSLHPGL GCVLSLPHRR LVCYCRLFEV PDPNKPQKLG LHQREIFLFN 670 680 690 700 710 720 DLLVVTKIFQ KKKNSVTYSF RQSFSLYGMQ VLLFENQYYP NGIRLTSSVP GADIKVLINF 730 740 750 760 770 780 NAPNPQDRKK FTDDLRESIA EVQEMEKHRI ESELEKQKGV VRPSMSQCSS LKKESGNGTL 790 800 810 820 830 840 SRACLDDSYA SGEGLKRSAL SSSLRDLSEA GKRGRRSSAG SLESNVEFQP FEPLQPSVLC S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins." Dunphy J.L., Moravec R., Ly K., Lasell T.K., Melancon P., Casanova J.E. Curr. Biol. 16:315-320(2006) [PubMed: 16461286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed: 9872452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-936 (ISOFORM 2). Tissue: Placenta.
[4]	"ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6." Someya A., Sata M., Takeda K., Pacheco-Rodriguez G., Ferrans V.J., Moss J., Vaughan M. Proc. Natl. Acad. Sci. U.S.A. 98:2413-2418(2001) [PubMed: 11226253] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ARF6.
[5]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-362, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-180 AND SER-512, MASS SPECTROMETRY.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY653734 mRNA. Translation: AAT72063.1. AB018306 mRNA. Translation: BAA34483.2. BC010267 mRNA. Translation: AAH10267.1.
IPI	IPI00457114. IPI00760711.
RefSeq	NP_055684.3.
UniGene	Hs.475506
3D structure databases
HSSP	HSSP built from PDB template 1R8Q based on UniProtKB Q99418.
ModBase	Search...
PTM databases
PhosphoSite	Q6DN90.
Proteomic databases
PRIDE	Q6DN90.
Genome annotation databases
Ensembl	ENSG00000144711. Homo sapiens. [Contig view]
GeneID	9922.
Organism-specific databases
GeneCards	GC03M012916.
HGNC	HGNC:29112. IQSEC1.
MIM	610166. gene.
PharmGKB	PA128394566.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q6DN90.
HOVERGEN	Q6DN90.
OMA	Q6DN90. LDSPSAY.
Enzyme and pathway databases
Pathway_Interaction_DB	arf6cyclingpathway. Arf6 signaling events.
Gene expression databases
ArrayExpress	Q6DN90.
Bgee	Q6DN90.
CleanEx	HS_IQSEC1.
GermOnline	ENSG00000144711. Homo sapiens.
Family and domain databases
InterPro	IPR000048. IQ_CaM_bd_region. IPR001849. Pleckstrin_homology. IPR000904. Sec7. [Graphical view]
Pfam	PF01369. Sec7. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00222. Sec7. 1 hit. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. PS50003. PH_DOMAIN. False negative. PS50190. SEC7. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	37432.
SOURCE	Search...

Entry information

Entry name

IQEC1_HUMAN

Accession

Primary (citable) accession number: Q6DN90
Secondary accession number(s): O94863, Q96D85

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)