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Q6DN04

- MIF_MACMU

UniProt

Q6DN04 - MIF_MACMU

Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
  1. Functioni

    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.By similarity

    Catalytic activityi

    Keto-phenylpyruvate = enol-phenylpyruvate.
    L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
    Binding sitei33 – 331SubstrateBy similarity
    Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
    Binding sitei98 – 981SubstrateBy similarity

    GO - Molecular functioni

    1. chemoattractant activity Source: Ensembl
    2. dopachrome isomerase activity Source: UniProtKB
    3. phenylpyruvate tautomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell aging Source: Ensembl
    2. cell proliferation Source: Ensembl
    3. cell surface receptor signaling pathway Source: Ensembl
    4. DNA damage response, signal transduction by p53 class mediator Source: Ensembl
    5. inflammatory response Source: UniProtKB-KW
    6. innate immune response Source: UniProtKB-KW
    7. negative regulation of cell aging Source: Ensembl
    8. negative regulation of cell cycle arrest Source: Ensembl
    9. negative regulation of cellular protein metabolic process Source: Ensembl
    10. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
    11. negative regulation of gene expression Source: Ensembl
    12. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
    13. negative regulation of mature B cell apoptotic process Source: Ensembl
    14. negative regulation of myeloid cell apoptotic process Source: Ensembl
    15. positive regulation of arachidonic acid secretion Source: Ensembl
    16. positive regulation of B cell proliferation Source: Ensembl
    17. positive regulation of chemokine (C-X-C motif) ligand 2 production Source: Ensembl
    18. positive regulation of cytokine secretion Source: Ensembl
    19. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
    20. positive regulation of fibroblast proliferation Source: Ensembl
    21. positive regulation of lipopolysaccharide-mediated signaling pathway Source: Ensembl
    22. positive regulation of MAP kinase activity Source: Ensembl
    23. positive regulation of myeloid leukocyte cytokine production involved in immune response Source: Ensembl
    24. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
    25. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    26. positive regulation of prostaglandin secretion involved in immune response Source: Ensembl
    27. positive regulation of protein kinase A signaling Source: Ensembl
    28. prostaglandin biosynthetic process Source: Ensembl
    29. protein homotrimerization Source: Ensembl

    Keywords - Molecular functioni

    Cytokine, Isomerase

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Macrophage migration inhibitory factor (EC:5.3.2.1)
    Short name:
    MIF
    Alternative name(s):
    L-dopachrome isomerase
    L-dopachrome tautomerase (EC:5.3.3.12)
    Phenylpyruvate tautomerase
    Gene namesi
    Name:MIF
    OrganismiMacaca mulatta (Rhesus macaque)
    Taxonomic identifieri9544 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
    ProteomesiUP000006718: Unplaced

    Subcellular locationi

    Secreted By similarity. Cytoplasm By similarity
    Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens By similarity.By similarity

    GO - Cellular componenti

    1. cell surface Source: Ensembl
    2. cytoplasm Source: UniProtKB-SubCell
    3. extracellular space Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000158064Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei78 – 781N6-acetyllysine; alternateBy similarity
    Modified residuei78 – 781N6-succinyllysine; alternateBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ6DN04.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with BNIPL By similarity. Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9544.ENSMMUP00000011949.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6DN04.
    SMRiQ6DN04. Positions 2-115.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MIF family.Curated

    Phylogenomic databases

    eggNOGiNOG08790.
    GeneTreeiENSGT00730000111101.
    HOGENOMiHOG000112325.
    HOVERGENiHBG003240.
    InParanoidiQ6DN04.
    KOiK07253.
    OMAiKPERYVM.
    OrthoDBiEOG7GXPDN.
    TreeFamiTF313853.

    Family and domain databases

    InterProiIPR001398. Macrophage_inhib_fac.
    IPR019829. Macrophage_inhib_fac_CS.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view]
    PANTHERiPTHR11954. PTHR11954. 1 hit.
    PfamiPF01187. MIF. 1 hit.
    [Graphical view]
    ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF55331. SSF55331. 1 hit.
    PROSITEiPS01158. MIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6DN04-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF    50
    GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY 100
    DMNAANVGWN NSTFA 115
    Length:115
    Mass (Da):12,476
    Last modified:January 23, 2007 - v4
    Checksum:i56D51107C05286B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY656809 mRNA. Translation: AAT74528.2.
    RefSeqiNP_001028087.1. NM_001032915.1.
    UniGeneiMmu.29.

    Genome annotation databases

    EnsembliENSMMUT00000012742; ENSMMUP00000011949; ENSMMUG00000009108.
    GeneIDi574298.
    KEGGimcc:574298.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY656809 mRNA. Translation: AAT74528.2 .
    RefSeqi NP_001028087.1. NM_001032915.1.
    UniGenei Mmu.29.

    3D structure databases

    ProteinModelPortali Q6DN04.
    SMRi Q6DN04. Positions 2-115.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9544.ENSMMUP00000011949.

    Chemistry

    BindingDBi Q6DN04.

    Proteomic databases

    PRIDEi Q6DN04.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMMUT00000012742 ; ENSMMUP00000011949 ; ENSMMUG00000009108 .
    GeneIDi 574298.
    KEGGi mcc:574298.

    Organism-specific databases

    CTDi 4282.

    Phylogenomic databases

    eggNOGi NOG08790.
    GeneTreei ENSGT00730000111101.
    HOGENOMi HOG000112325.
    HOVERGENi HBG003240.
    InParanoidi Q6DN04.
    KOi K07253.
    OMAi KPERYVM.
    OrthoDBi EOG7GXPDN.
    TreeFami TF313853.

    Miscellaneous databases

    NextBioi 19977458.

    Family and domain databases

    InterProi IPR001398. Macrophage_inhib_fac.
    IPR019829. Macrophage_inhib_fac_CS.
    IPR014347. Tautomerase/MIF_sf.
    [Graphical view ]
    PANTHERi PTHR11954. PTHR11954. 1 hit.
    Pfami PF01187. MIF. 1 hit.
    [Graphical view ]
    ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF55331. SSF55331. 1 hit.
    PROSITEi PS01158. MIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rhesus monkey DNA sequencing."
      Cao W.G., Jia Y.B., Slayden O.V., Mah K., Carroll R.S., Brenner R.M.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiMIF_MACMU
    AccessioniPrimary (citable) accession number: Q6DN04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 77 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3