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Q6DN04

- MIF_MACMU

UniProt

Q6DN04 - MIF_MACMU

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Protein

Macrophage migration inhibitory factor

Gene

MIF

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (By similarity).By similarity

Catalytic activityi

Keto-phenylpyruvate = enol-phenylpyruvate.
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
Binding sitei33 – 331SubstrateBy similarity
Binding sitei65 – 651Substrate; via amide nitrogenBy similarity
Binding sitei98 – 981SubstrateBy similarity

GO - Molecular functioni

  1. chemoattractant activity Source: Ensembl
  2. dopachrome isomerase activity Source: UniProtKB
  3. phenylpyruvate tautomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell aging Source: Ensembl
  2. cell proliferation Source: Ensembl
  3. cell surface receptor signaling pathway Source: Ensembl
  4. DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  5. inflammatory response Source: UniProtKB-KW
  6. innate immune response Source: UniProtKB-KW
  7. negative regulation of cell aging Source: Ensembl
  8. negative regulation of cell cycle arrest Source: Ensembl
  9. negative regulation of cellular protein metabolic process Source: Ensembl
  10. negative regulation of DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  11. negative regulation of gene expression Source: Ensembl
  12. negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: Ensembl
  13. negative regulation of mature B cell apoptotic process Source: Ensembl
  14. negative regulation of myeloid cell apoptotic process Source: Ensembl
  15. positive regulation of arachidonic acid secretion Source: Ensembl
  16. positive regulation of B cell proliferation Source: Ensembl
  17. positive regulation of chemokine (C-X-C motif) ligand 2 production Source: Ensembl
  18. positive regulation of cytokine secretion Source: Ensembl
  19. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  20. positive regulation of fibroblast proliferation Source: Ensembl
  21. positive regulation of lipopolysaccharide-mediated signaling pathway Source: Ensembl
  22. positive regulation of MAP kinase activity Source: Ensembl
  23. positive regulation of myeloid leukocyte cytokine production involved in immune response Source: Ensembl
  24. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
  25. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  26. positive regulation of prostaglandin secretion involved in immune response Source: Ensembl
  27. positive regulation of protein kinase A signaling Source: Ensembl
  28. prostaglandin biosynthetic process Source: Ensembl
  29. protein homotrimerization Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor (EC:5.3.2.1)
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
Name:MIF
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
ProteomesiUP000006718: Unplaced

Subcellular locationi

Secreted By similarity. Cytoplasm By similarity
Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens (By similarity).By similarity

GO - Cellular componenti

  1. cell surface Source: Ensembl
  2. cytoplasm Source: UniProtKB-KW
  3. extracellular space Source: UniProtKB-KW
  4. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 115114Macrophage migration inhibitory factorPRO_0000158064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781N6-acetyllysine; alternateBy similarity
Modified residuei78 – 781N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ6DN04.

Interactioni

Subunit structurei

Homotrimer. Interacts with BNIPL (By similarity). Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain (By similarity).By similarity

Protein-protein interaction databases

STRINGi9544.ENSMMUP00000011949.

Structurei

3D structure databases

ProteinModelPortaliQ6DN04.
SMRiQ6DN04. Positions 2-115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

eggNOGiNOG08790.
GeneTreeiENSGT00730000111101.
HOGENOMiHOG000112325.
HOVERGENiHBG003240.
InParanoidiQ6DN04.
KOiK07253.
OMAiKPERYVM.
OrthoDBiEOG7GXPDN.
TreeFamiTF313853.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6DN04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF
60 70 80 90 100
GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY
110
DMNAANVGWN NSTFA
Length:115
Mass (Da):12,476
Last modified:January 23, 2007 - v4
Checksum:i56D51107C05286B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY656809 mRNA. Translation: AAT74528.2.
RefSeqiNP_001028087.1. NM_001032915.1.
UniGeneiMmu.29.

Genome annotation databases

EnsembliENSMMUT00000012742; ENSMMUP00000011949; ENSMMUG00000009108.
GeneIDi574298.
KEGGimcc:574298.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY656809 mRNA. Translation: AAT74528.2 .
RefSeqi NP_001028087.1. NM_001032915.1.
UniGenei Mmu.29.

3D structure databases

ProteinModelPortali Q6DN04.
SMRi Q6DN04. Positions 2-115.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9544.ENSMMUP00000011949.

Chemistry

BindingDBi Q6DN04.

Proteomic databases

PRIDEi Q6DN04.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMMUT00000012742 ; ENSMMUP00000011949 ; ENSMMUG00000009108 .
GeneIDi 574298.
KEGGi mcc:574298.

Organism-specific databases

CTDi 4282.

Phylogenomic databases

eggNOGi NOG08790.
GeneTreei ENSGT00730000111101.
HOGENOMi HOG000112325.
HOVERGENi HBG003240.
InParanoidi Q6DN04.
KOi K07253.
OMAi KPERYVM.
OrthoDBi EOG7GXPDN.
TreeFami TF313853.

Miscellaneous databases

NextBioi 19977458.

Family and domain databases

InterProi IPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view ]
PANTHERi PTHR11954. PTHR11954. 1 hit.
Pfami PF01187. MIF. 1 hit.
[Graphical view ]
ProDomi PD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF55331. SSF55331. 1 hit.
PROSITEi PS01158. MIF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rhesus monkey DNA sequencing."
    Cao W.G., Jia Y.B., Slayden O.V., Mah K., Carroll R.S., Brenner R.M.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiMIF_MACMU
AccessioniPrimary (citable) accession number: Q6DN04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 78 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3