Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6DN04 (MIF_MACMU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Macrophage migration inhibitory factor

Short name=MIF
EC=5.3.2.1
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase
EC=5.3.3.12
Phenylpyruvate tautomerase
Gene names
Name:MIF
OrganismMacaca mulatta (Rhesus macaque) [Reference proteome]
Taxonomic identifier9544 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Protein attributes

Sequence length115 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity By similarity.

Catalytic activity

Keto-phenylpyruvate = enol-phenylpyruvate.

L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.

Subunit structure

Homotrimer. Interacts with BNIPL By similarity. Interacts with the CD74 extracellular domain. Interacts with COPS5 and with the CXCR2 extracellular domain By similarity.

Subcellular location

Secreted By similarity. Cytoplasm By similarity. Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens By similarity.

Sequence similarities

Belongs to the MIF family.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentCytoplasm
Secreted
   Molecular functionCytokine
Isomerase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA damage response, signal transduction by p53 class mediator

Inferred from electronic annotation. Source: Ensembl

cell aging

Inferred from electronic annotation. Source: Ensembl

cell proliferation

Inferred from electronic annotation. Source: Ensembl

cell surface receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of DNA damage response, signal transduction by p53 class mediator

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell aging

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell cycle arrest

Inferred from electronic annotation. Source: Ensembl

negative regulation of cellular protein metabolic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

Inferred from electronic annotation. Source: Ensembl

negative regulation of mature B cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of myeloid cell apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of B cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of MAP kinase activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of arachidonic acid secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of chemokine (C-X-C motif) ligand 2 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytokine secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of fibroblast proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of lipopolysaccharide-mediated signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of myeloid leukocyte cytokine production involved in immune response

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-serine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of prostaglandin secretion involved in immune response

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase A signaling

Inferred from electronic annotation. Source: Ensembl

prostaglandin biosynthetic process

Inferred from electronic annotation. Source: Ensembl

protein homotrimerization

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionchemoattractant activity

Inferred from electronic annotation. Source: Ensembl

dopachrome isomerase activity

Inferred from sequence or structural similarity. Source: UniProtKB

phenylpyruvate tautomerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 115114Macrophage migration inhibitory factor
PRO_0000158064

Sites

Active site21Proton acceptor; via imino nitrogen By similarity
Binding site331Substrate By similarity
Binding site651Substrate; via amide nitrogen By similarity
Binding site981Substrate By similarity

Amino acid modifications

Modified residue781N6-acetyllysine; alternate By similarity
Modified residue781N6-succinyllysine; alternate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6DN04 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 56D51107C05286B2

FASTA11512,476
        10         20         30         40         50         60 
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC 

        70         80         90        100        110 
SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA 

« Hide

References

[1]"Rhesus monkey DNA sequencing."
Cao W.G., Jia Y.B., Slayden O.V., Mah K., Carroll R.S., Brenner R.M.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY656809 mRNA. Translation: AAT74528.2.
RefSeqNP_001028087.1. NM_001032915.1.
UniGeneMmu.29.

3D structure databases

ProteinModelPortalQ6DN04.
SMRQ6DN04. Positions 2-115.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9544.ENSMMUP00000011949.

Chemistry

BindingDBQ6DN04.

Proteomic databases

PRIDEQ6DN04.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMMUT00000012742; ENSMMUP00000011949; ENSMMUG00000009108.
GeneID574298.
KEGGmcc:574298.

Organism-specific databases

CTD4282.

Phylogenomic databases

eggNOGNOG08790.
GeneTreeENSGT00730000111101.
HOGENOMHOG000112325.
HOVERGENHBG003240.
InParanoidQ6DN04.
KOK07253.
OMAPDRIYIN.
OrthoDBEOG7GXPDN.
TreeFamTF313853.

Family and domain databases

InterProIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERPTHR11954. PTHR11954. 1 hit.
PfamPF01187. MIF. 1 hit.
[Graphical view]
ProDomPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF55331. SSF55331. 1 hit.
PROSITEPS01158. MIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio19977458.

Entry information

Entry nameMIF_MACMU
AccessionPrimary (citable) accession number: Q6DN04
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 75 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families