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Q6DKJ4 (NXN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoredoxin

EC=1.8.1.8
Gene names
Name:NXN
Synonyms:NRX
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a redox-dependent negative regulator of the Wnt signaling pathway, possibly by preventing ubiquitination of DVL3 by the BCR(KLHL12) complex. May also function as a transcriptional regulator act as a regulator of protein phosphatase 2A (PP2A) By similarity.

Catalytic activity

Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H.

Subunit structure

Associates with the phosphatase 2A holoenzyme. Interacts with PPP2CA; the interaction is direct. Interacts with DVL1 (via PDZ domain); the interaction is direct and regulated by oxidative stress By similarity. Ref.5

Subcellular location

Cytoplasmcytosol By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the nucleoredoxin family.

Contains 1 thioredoxin domain.

Sequence caution

The sequence BAB14171.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAB55122.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6DKJ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6DKJ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-113: Missing.
     114-120: YKEKHRK → MQELRNS
     275-290: IPTLIMLDPQGEVITR → RRSRPRLRVAPGNLLT
     291-435: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q6DKJ4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.
     109-120: WLALPYKEKHRK → MADVSLHRNPAT

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 435434Nucleoredoxin
PRO_0000332933

Regions

Domain167 – 321155Thioredoxin

Amino acid modifications

Modified residue21N-acetylserine Ref.6

Natural variations

Alternative sequence1 – 113113Missing in isoform 2.
VSP_033392
Alternative sequence1 – 108108Missing in isoform 3.
VSP_042792
Alternative sequence109 – 12012WLALP…EKHRK → MADVSLHRNPAT in isoform 3.
VSP_042793
Alternative sequence114 – 1207YKEKHRK → MQELRNS in isoform 2.
VSP_033393
Alternative sequence275 – 29016IPTLI…EVITR → RRSRPRLRVAPGNLLT in isoform 2.
VSP_033394
Alternative sequence291 – 435145Missing in isoform 2.
VSP_033395

Experimental info

Sequence conflict1241W → K in BAB55122. Ref.1
Sequence conflict1651W → K in BAB14171. Ref.1
Sequence conflict3721V → I in AAH73845. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: B67927F001BE4E5B

FASTA43548,392
        10         20         30         40         50         60 
MSGFLEELLG EKLVTGGGEE VDVHSLGARG ISLLGLYFGC SLSAPCAQLS ASLAAFYGRL 

        70         80         90        100        110        120 
RGDAAAGPGP GAGAGAAAEP EPRRRLEIVF VSSDQDQRQW QDFVRDMPWL ALPYKEKHRK 

       130        140        150        160        170        180 
LKLWNKYRIS NIPSLIFLDA TTGKVVCRNG LLVIRDDPEG LEFPWGPKPF REVIAGPLLR 

       190        200        210        220        230        240 
NNGQSLESSS LEGSHVGVYF SAHWCPPCRS LTRVLVESYR KIKEAGQNFE IIFVSADRSE 

       250        260        270        280        290        300 
ESFKQYFSEM PWLAVPYTDE ARRSRLNRLY GIQGIPTLIM LDPQGEVITR QGRVEVLNDE 

       310        320        330        340        350        360 
DCREFPWHPK PVLELSDSNA AQLNEGPCLV LFVDSEDDGE SEAAKQLIQP IAEKIIAKYK 

       370        380        390        400        410        420 
AKEEEAPLLF FVAGEDDMTD SLRDYTNLPE AAPLLTILDM SARAKYVMDV EEITPAIVEA 

       430 
FVNDFLAEKL KPEPI 

« Hide

Isoform 2 [UniParc].

Checksum: D36156E815E83ACF
Show »

FASTA17720,328
Isoform 3 [UniParc].

Checksum: A9343547616F7A95
Show »

FASTA32736,871

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 124-435 (ISOFORM 1).
Tissue: Teratocarcinoma and Testis.
[2]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 171-435 (ISOFORM 1).
Tissue: Brain, Liver, Lung and Uterus.
[5]"Interaction of nucleoredoxin with protein phosphatase 2A."
Lechward K., Sugajska E., de Baere I., Goris J., Hemmings B.A., Zolnierowicz S.
FEBS Lett. 580:3631-3637(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A COMPLEX WITH PHOSPHATASE 2A.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK022676 mRNA. Translation: BAB14171.1. Different initiation.
AK027451 mRNA. Translation: BAB55122.1. Different initiation.
AK302073 mRNA. Translation: BAG63462.1.
AC015884 Genomic DNA. No translation available.
AC036164 Genomic DNA. No translation available.
AC087392 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90640.1.
CH471108 Genomic DNA. Translation: EAW90641.1.
BC009327 mRNA. Translation: AAH09327.2.
BC063828 mRNA. Translation: AAH63828.1.
BC073845 mRNA. Translation: AAH73845.1.
BC104634 mRNA. Translation: AAI04635.1.
RefSeqNP_001192248.1. NM_001205319.1.
NP_071908.2. NM_022463.4.
UniGeneHs.527989.

3D structure databases

ProteinModelPortalQ6DKJ4.
SMRQ6DKJ4. Positions 2-165, 196-296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122144. 4 interactions.
IntActQ6DKJ4. 2 interactions.
MINTMINT-4898529.
STRING9606.ENSP00000337443.

PTM databases

PhosphoSiteQ6DKJ4.

Polymorphism databases

DMDM187471109.

Proteomic databases

PaxDbQ6DKJ4.
PeptideAtlasQ6DKJ4.
PRIDEQ6DKJ4.

Protocols and materials databases

DNASU64359.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336868; ENSP00000337443; ENSG00000167693. [Q6DKJ4-1]
ENST00000537628; ENSP00000446446; ENSG00000167693.
ENST00000575801; ENSP00000461038; ENSG00000167693. [Q6DKJ4-3]
GeneID64359.
KEGGhsa:64359.
UCSCuc002fsa.3. human. [Q6DKJ4-1]
uc010vqe.2. human. [Q6DKJ4-3]

Organism-specific databases

CTD64359.
GeneCardsGC17M000702.
H-InvDBHIX0013394.
HGNCHGNC:18008. NXN.
HPAHPA022850.
HPA023566.
MIM612895. gene.
neXtProtNX_Q6DKJ4.
PharmGKBPA31863.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG273116.
HOGENOMHOG000007309.
HOVERGENHBG053461.
InParanoidQ6DKJ4.
KOK17609.
OMAWQDFVRD.
OrthoDBEOG70W3G2.
PhylomeDBQ6DKJ4.
TreeFamTF331873.

Gene expression databases

ArrayExpressQ6DKJ4.
BgeeQ6DKJ4.
CleanExHS_NXN.
GenevestigatorQ6DKJ4.

Family and domain databases

Gene3D3.40.30.10. 2 hits.
InterProIPR012336. Thioredoxin-like_fold.
[Graphical view]
SUPFAMSSF52833. SSF52833. 3 hits.
PROSITEPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNXN. human.
GenomeRNAi64359.
NextBio66291.
PROQ6DKJ4.
SOURCESearch...

Entry information

Entry nameNXN_HUMAN
AccessionPrimary (citable) accession number: Q6DKJ4
Secondary accession number(s): B4DXQ0 expand/collapse secondary AC list , D3DTH2, Q3SWW6, Q6P3U6, Q7L4C6, Q9H9Q1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: March 19, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM