Q6DKB0 (5NTC_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic purine 5'-nucleotidase
EC=3.1.3.5

Alternative name(s):
Cytosolic 5'-nucleotidase II

Gene names

Name:

nt5c2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Enzyme regulation	Allosterically activated by various compounds, including ATP By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the 5'(3')-deoxyribonucleotidase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Cytosolic purine 5'-nucleotidase		PRO_0000310267
Regions
	Region	202 – 210	9	Substrate binding Potential
	Compositional bias	549 – 569	21	Asp/Glu-rich (acidic)
Sites
	Active site	52	1	Nucleophile By similarity
	Active site	54	1	Proton donor By similarity
	Metal binding	52	1	Magnesium By similarity
	Metal binding	54	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	351	1	Magnesium By similarity
	Binding site	127	1	Allosteric activator 1 By similarity
	Binding site	154	1	Allosteric activator 2 By similarity
	Binding site	354	1	Allosteric activator 2 By similarity
	Binding site	436	1	Allosteric activator 1; via carbonyl oxygen By similarity
	Binding site	453	1	Allosteric activator 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DKB0 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: E181B6EAF7455227	FASTA	569	66,180
	10 20 30 40 50 60 MTTSWSDRLQ NAADLPANMD RHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY 70 80 90 100 110 120 KSPEYESLGF DLTVERLVSI GYPQELLNFV YDPTFPTRGL VFDSTYGNLI KVDAYGNILV 130 140 150 160 170 180 CAHGFNFMRG LETREQYPNK FIQRDDTDRF YILNTLFNLP ETYLLACLVD FFTNCDRYSS 190 200 210 220 230 240 CETGFKDGDL FMSFRSMFQD VRDAVDWVHY KGSLKEKTVE NLPKYVVKDS KLPLLLSRMN 250 260 270 280 290 300 EVGKVFLVTN SDYKYTHKIM TYLFDLPHGP KPDSSHRVWQ SYFDLILVDA RKPLFFGEGT 310 320 330 340 350 360 VLRQVDTNTG KLKIGTYTGP LQHGIVYSGG SSDILCDLLG AKGKDILYIG DHIFGDILKS 370 380 390 400 410 420 KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ 430 440 450 460 470 480 RRVKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA 490 500 510 520 530 540 HVLMPHESTV EHAHVDIHES ESPMATRNRC SLDFKDSEFK RHHLTRSISE IKPPNLFPQK 550 560 PREITHCHDE DDDEEEEEEE EEEEEEEEE

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC074446 mRNA. Translation: AAH74446.1.
RefSeq	NP_001086300.1. NM_001092831.1.
UniGene	Xl.9923.
3D structure databases
HSSP	HSSP built from PDB template 2BDE based on UniProtKB Q5ZZB6.
ProteinModelPortal	Q6DKB0.
SMR	Q6DKB0. Positions 3-488.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	444729.
KEGG	xla:444729.
Organism-specific databases
CTD	22978.
Xenbase	XB-GENE-988880. nt5c2.
Phylogenomic databases
HOVERGEN	HBG000025.
Family and domain databases
InterPro	IPR023214. HAD-like_dom. IPR008380. HAD-SF_hydro_IG_5-nucl. IPR016695. Pur_nucleotidase. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 3 hits.
KO	K01081.
PANTHER	PTHR12103. HAD-SF_hydro_IG_5-nucl. 1 hit.
Pfam	PF05761. 5_nucleotid. 1 hit. [Graphical view]
PIRSF	PIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR02244. HAD-IG-Ncltidse. 1 hit.
ProtoNet	Search...

Entry information

Entry name

5NTC_XENLA

Accession

Primary (citable) accession number: Q6DKB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	August 16, 2004
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program