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Q6DJT9

- PLAG1_HUMAN

UniProt

Q6DJT9 - PLAG1_HUMAN

Protein

Zinc finger protein PLAG1

Gene

PLAG1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri34 – 5623C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri62 – 8625C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri92 – 11423C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri121 – 14323C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri150 – 17223C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri185 – 20723C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri213 – 23624C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    4. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
    5. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. gland morphogenesis Source: Ensembl
    2. multicellular organism growth Source: Ensembl
    3. negative regulation of gene expression Source: Ensembl
    4. organ growth Source: Ensembl
    5. positive regulation of glial cell proliferation Source: Ensembl
    6. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    7. prostate gland growth Source: Ensembl
    8. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    SignaLinkiQ6DJT9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein PLAG1
    Alternative name(s):
    Pleiomorphic adenoma gene 1 protein
    Gene namesi
    Name:PLAG1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:9045. PLAG1.

    Subcellular locationi

    Nucleus 3 Publications
    Note: Strong nucleolar localization when sumoylation is inhibited.

    GO - Cellular componenti

    1. nucleus Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    A chromosomal aberration involving PLAG1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with constituvely expressed beta-catenin/CTNNB1. Fusion occurs in the 5'-regulatory regions, leading to promoter swapping between the 2 genes and activation of PLAG1 expression in adenomas. The chimeric transcript is formed by fusion of CTNNB1 exon 1 to PLAG1 exon 3. Reciprocal fusion transcript consisting of PLAG1 exon 1 and CTNNB1 exon 2-16 is also revealed in some adenomas. Translocation t(3;8)(p21;q12) with transcription elongation factor SII/TCEA1. The fusion transcript is composed of 5'-non-coding sequences as well as 63 nucleotides of the coding region of TCEA1 fused to the acceptor splice site of PLAG1 exon 3. The fusion transcript encodes a truncated TCEA1-PLAG1 protein of 90 AA as well as an apparently normal PLAG1 protein. Reciprocal fusion transcript PLAG1-TCEA1 is also present in one adenoma. Translocation t(5;8)(p13;q12) with leukemia inhibitory factor receptor LIFR. This fusion occured in the 5'-non-coding sequences of both genes, exchanging regulatory control element while preserving the coding sequences. Translocation t(6;8)(p21.3-22;q13) with Coiled-coil-helix-coiled-coil-helix domain-containing protein 7/CHCHD7. Fusion occurs in the 5' regulatory regions, leading to promoter swapping and up-regulation of PLAG1 expression. Ectopic expression of PLAG1 under the control of promoters of distinct translocation partner genes is a general pathogenetic mechanism for pleiomorphic adenomas with 8q aberrations. These fusion genes are likely to be found in adenomas with normal karyotype as this subgroup of tumors also exhibit PLAG1 activation.
    A chromosomal aberration involving PLAG1 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. 8q12.1 to 8q24.1 intrachromosomal rearrangement with hyaluronic acid synthase 2/HAS2 results in promoter swapping and activation of PLAG1 expression. The breakpoint of HAS2 gene is in PLAG1 intron 1, whereas its coding sequence starts at exon 2 or exon 3. Translocation t(7;8)(p22;q13) with collagen 1A2/COL1A2. Fusion transcript COL1A2-PLAG1 as well as HAS2-PLAG1 encode a full-length PLAG1 protein.

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi23 – 242RK → AA: Inhibition of KPNA2 interaction when mutation occurs in the NLS; decreased nuclear import with localization in the nucleus but also in the cytoplasm; Complete inhibition of nuclear import when associated with a lack of zinc-finger domains. 1 Publication
    Mutagenesisi31 – 322RK → AA: No inhibition of KPNA2 interaction and no change in nuclear import. 1 Publication
    Mutagenesisi92 – 921H → A: Prevents formation of functional zinc-finger 3; induces drastic decrease of DNA affinity and complete modification of DNA binding specificity. 2 Publications
    Mutagenesisi227 – 2271H → A: Prevents formation of functional zinc-finger 7 and inhibits DNA binding; No proliferation and transformation of cultured cells. 3 Publications
    Mutagenesisi244 – 2441K → R: Abolishes single and double sumoylation; nuclear localization conserved. Increases transcriptional activity and inhibits repression domain activity; when associated with R-263 and R-353. 3 Publications
    Mutagenesisi263 – 2631K → R: Decreases sumoylation; Abolishes double sumoylation only; Nuclear localization conserved. Increases transcriptional activity and inhibits repression domain activity; when associated with R-244 and R-353. 3 Publications
    Mutagenesisi339 – 3391T → A: No effect on transcription activation capacity. 2 Publications
    Mutagenesisi340 – 3401S → A: No effect on transcription activation capacity. 2 Publications
    Mutagenesisi353 – 3531K → R: No effect on sumoylation. Increases transcriptional activity and inhibits repression domain activity; when associated with R-244 and R-263. 3 Publications

    Keywords - Diseasei

    Proto-oncogene

    Organism-specific databases

    MIMi181030. phenotype.
    PharmGKBiPA33378.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Zinc finger protein PLAG1PRO_0000295107Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki244 – 244Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
    Cross-linki263 – 263Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

    Post-translational modificationi

    Sumoylated with SUMO1; which inhibits transcriptional activity, but does not affect nuclear localization. Blockers of sumoylation pathway such as SENP3 and inactive UBE2I increases transcriptional capacity. Sumoylation is increased in the presence of PIAS1.
    Acetylated by lysine acetyltransferase EP300; which activates transcriptional capacity. Lysine residues that are sumoylated also seem to be target for acetylation.1 Publication

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiQ6DJT9.
    PaxDbiQ6DJT9.
    PRIDEiQ6DJT9.

    PTM databases

    PhosphoSiteiQ6DJT9.

    Expressioni

    Tissue specificityi

    Expressed in fetal tissues such as lung, liver and kidney. Not detected or weak detection in normal adult tissues, but highly expressed in salivary gland with benign or malignant pleiomorphic adenomas with or without 8q12 aberrations, with preferential occurrence in benign tumors.4 Publications

    Gene expression databases

    ArrayExpressiQ6DJT9.
    BgeeiQ6DJT9.
    CleanExiHS_PLAG1.
    GenevestigatoriQ6DJT9.

    Interactioni

    Subunit structurei

    Interacts with KPNA2, which escorts protein to the nucleus via interaction with nuclear localization signal. Interacts with E3 SUMO-protein ligase PIAS1, PIAS2 and PIAS4.2 Publications

    Protein-protein interaction databases

    BioGridi111340. 7 interactions.
    IntActiQ6DJT9. 3 interactions.
    MINTiMINT-1188755.
    STRINGi9606.ENSP00000325546.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6DJT9.
    SMRiQ6DJT9. Positions 32-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 8483Interacts with KPNA2Add
    BLAST
    Regioni41 – 242202Decreased nuclear import with localization in the nucleus but also in the cytoplasmAdd
    BLAST
    Regioni243 – 500258Activates transcription; Inhibition of nuclear import due to lack of NLS and KPNA2 interactionAdd
    BLAST
    Regioni243 – 384142Repression domain; contains 3 sumoylation motifs and massively decrease transcription activityAdd
    BLAST
    Regioni385 – 500116Massively activates transcriptionAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi22 – 254Nuclear localization signal

    Domaini

    C2H2-type zinc fingers 3 interacts with DNA-binding site G-clusterinc fingers. C2H2-type zinc fingers 6 and 7 interact with DNA-binding site core sequence.

    Sequence similaritiesi

    Contains 7 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri34 – 5623C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri62 – 8625C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri92 – 11423C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri121 – 14323C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri150 – 17223C2H2-type 5PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri185 – 20723C2H2-type 6PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri213 – 23624C2H2-type 7PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    HOGENOMiHOG000294191.
    HOVERGENiHBG053608.
    InParanoidiQ6DJT9.
    OMAiIDMDAVH.
    OrthoDBiEOG71K62Q.
    PhylomeDBiQ6DJT9.
    TreeFamiTF332024.

    Family and domain databases

    Gene3Di3.30.160.60. 5 hits.
    InterProiIPR027775. C2H2_Znf_fam.
    IPR027765. PLAG1.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PANTHERiPTHR10032. PTHR10032. 1 hit.
    PTHR10032:SF124. PTHR10032:SF124. 1 hit.
    PfamiPF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 7 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 7 hits.
    PS50157. ZINC_FINGER_C2H2_2. 7 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6DJT9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATVIPGDLS EVRDTQKVPS GKRKRGETKP RKNFPCQLCD KAFNSVEKLK    50
    VHSYSHTGER PYKCIQQDCT KAFVSKYKLQ RHMATHSPEK THKCNYCEKM 100
    FHRKDHLKNH LHTHDPNKET FKCEECGKNY NTKLGFKRHL ALHAATSGDL 150
    TCKVCLQTFE STGVLLEHLK SHAGKSSGGV KEKKHQCEHC DRRFYTRKDV 200
    RRHMVVHTGR KDFLCQYCAQ RFGRKDHLTR HMKKSHNQEL LKVKTEPVDF 250
    LDPFTCNVSV PIKDELLPVM SLPSSELLSK PFTNTLQLNL YNTPFQSMQS 300
    SGSAHQMITT LPLGMTCPID MDTVHPSHHL SFKYPFSSTS YAISIPEKEQ 350
    PLKGEIESYL MELQGGVPSS SQDSQASSSS KLGLDPQIGS LDDGAGDLSL 400
    SKSSISISDP LNTPALDFSQ LFNFIPLNGP PYNPLSVGSL GMSYSQEEAH 450
    SSVSQLPPQT QDLQDPANTI GLGSLHSLSA AFTSSLSTST TLPRFHQAFQ 500
    Length:500
    Mass (Da):55,909
    Last modified:August 16, 2004 - v1
    Checksum:iCF022A132A1BFD43
    GO
    Isoform 2 (identifier: Q6DJT9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-82: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:418
    Mass (Da):46,474
    Checksum:i00BA4A605A99B39C
    GO

    Sequence cautioni

    The sequence BAD92368.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti276 – 2761E → D in BAD92368. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti458 – 4581P → T.1 Publication
    Corresponds to variant rs35883156 [ dbSNP | Ensembl ].
    VAR_033212

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8282Missing in isoform 2. 1 PublicationVSP_045183Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65002 mRNA. Translation: AAC50995.1.
    AK296933 mRNA. Translation: BAG59484.1.
    AB209131 mRNA. Translation: BAD92368.1. Different initiation.
    AC107952 Genomic DNA. No translation available.
    BC075047 mRNA. Translation: AAH75047.1.
    BC075048 mRNA. Translation: AAH75048.1.
    CCDSiCCDS47860.1. [Q6DJT9-2]
    CCDS6165.1. [Q6DJT9-1]
    RefSeqiNP_001108106.1. NM_001114634.1. [Q6DJT9-1]
    NP_001108107.1. NM_001114635.1. [Q6DJT9-2]
    NP_002646.2. NM_002655.2. [Q6DJT9-1]
    XP_005251317.1. XM_005251260.1. [Q6DJT9-1]
    UniGeneiHs.14968.

    Genome annotation databases

    EnsembliENST00000316981; ENSP00000325546; ENSG00000181690. [Q6DJT9-1]
    ENST00000423799; ENSP00000404067; ENSG00000181690. [Q6DJT9-2]
    ENST00000429357; ENSP00000416537; ENSG00000181690. [Q6DJT9-1]
    GeneIDi5324.
    KEGGihsa:5324.
    UCSCiuc003xsq.4. human. [Q6DJT9-1]

    Polymorphism databases

    DMDMi74757442.

    Keywords - Coding sequence diversityi

    Alternative splicing, Chromosomal rearrangement, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65002 mRNA. Translation: AAC50995.1 .
    AK296933 mRNA. Translation: BAG59484.1 .
    AB209131 mRNA. Translation: BAD92368.1 . Different initiation.
    AC107952 Genomic DNA. No translation available.
    BC075047 mRNA. Translation: AAH75047.1 .
    BC075048 mRNA. Translation: AAH75048.1 .
    CCDSi CCDS47860.1. [Q6DJT9-2 ]
    CCDS6165.1. [Q6DJT9-1 ]
    RefSeqi NP_001108106.1. NM_001114634.1. [Q6DJT9-1 ]
    NP_001108107.1. NM_001114635.1. [Q6DJT9-2 ]
    NP_002646.2. NM_002655.2. [Q6DJT9-1 ]
    XP_005251317.1. XM_005251260.1. [Q6DJT9-1 ]
    UniGenei Hs.14968.

    3D structure databases

    ProteinModelPortali Q6DJT9.
    SMRi Q6DJT9. Positions 32-239.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111340. 7 interactions.
    IntActi Q6DJT9. 3 interactions.
    MINTi MINT-1188755.
    STRINGi 9606.ENSP00000325546.

    PTM databases

    PhosphoSitei Q6DJT9.

    Polymorphism databases

    DMDMi 74757442.

    Proteomic databases

    MaxQBi Q6DJT9.
    PaxDbi Q6DJT9.
    PRIDEi Q6DJT9.

    Protocols and materials databases

    DNASUi 5324.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000316981 ; ENSP00000325546 ; ENSG00000181690 . [Q6DJT9-1 ]
    ENST00000423799 ; ENSP00000404067 ; ENSG00000181690 . [Q6DJT9-2 ]
    ENST00000429357 ; ENSP00000416537 ; ENSG00000181690 . [Q6DJT9-1 ]
    GeneIDi 5324.
    KEGGi hsa:5324.
    UCSCi uc003xsq.4. human. [Q6DJT9-1 ]

    Organism-specific databases

    CTDi 5324.
    GeneCardsi GC08M057073.
    HGNCi HGNC:9045. PLAG1.
    MIMi 181030. phenotype.
    603026. gene.
    neXtProti NX_Q6DJT9.
    PharmGKBi PA33378.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5048.
    HOGENOMi HOG000294191.
    HOVERGENi HBG053608.
    InParanoidi Q6DJT9.
    OMAi IDMDAVH.
    OrthoDBi EOG71K62Q.
    PhylomeDBi Q6DJT9.
    TreeFami TF332024.

    Enzyme and pathway databases

    SignaLinki Q6DJT9.

    Miscellaneous databases

    ChiTaRSi PLAG1. human.
    GeneWikii PLAG1.
    GenomeRNAii 5324.
    NextBioi 20594.
    PROi Q6DJT9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6DJT9.
    Bgeei Q6DJT9.
    CleanExi HS_PLAG1.
    Genevestigatori Q6DJT9.

    Family and domain databases

    Gene3Di 3.30.160.60. 5 hits.
    InterProi IPR027775. C2H2_Znf_fam.
    IPR027765. PLAG1.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    PANTHERi PTHR10032. PTHR10032. 1 hit.
    PTHR10032:SF124. PTHR10032:SF124. 1 hit.
    Pfami PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 7 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 7 hits.
    PS50157. ZINC_FINGER_C2H2_2. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Promoter swapping between the genes for a novel zinc finger protein and beta-catenin in pleiomorphic adenomas with t(3;8)(p21;q12) translocations."
      Kas K., Voz M.L., Roeijer E., Astroem A.-K., Meyen E., Stenman G., Van de Ven W.J.M.
      Nat. Genet. 15:170-174(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, CHROMOSOMAL TRANSLOCATION WITH CTNNB1, VARIANT THR-458.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Tongue.
    3. Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "DNA sequence and analysis of human chromosome 8."
      Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
      , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
      Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    6. "Transcriptional activation capacity of the novel PLAG family of zinc finger proteins."
      Kas K., Voz M.L., Hensen K., Meyen E., Van de Ven W.J.M.
      J. Biol. Chem. 273:23026-23032(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, MUTAGENESIS OF THR-339 AND SER-340.
    7. "The recurrent translocation t(5;8)(p13;q12) in pleomorphic adenomas results in upregulation of PLAG1 gene expression under control of the LIFR promoter."
      Voz M.L., Astrom A.-K., Kas K., Mark J., Stenman G., Van de Ven W.J.M.
      Oncogene 16:1409-1416(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHROMOSOMAL TRANSLOCATION WITH LIFR.
    8. "Conserved mechanism of PLAG1 activation in salivary gland tumors with and without chromosome 8q12 abnormalities: identification of SII as a new fusion partner gene."
      Astroem A.-K., Voz M.L., Kas K., Roeijer E., Wedell B., Mandahl N., Van de Ven W., Mark J., Stenman G.
      Cancer Res. 59:918-923(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHROMOSOMAL TRANSLOCATION WITH CTNNB1 AND TCEA1, TISSUE SPECIFICITY.
    9. "PLAG1, the main translocation target in pleomorphic adenoma of the salivary glands, is a positive regulator of IGF-II."
      Voz M.L., Agten N.S., Van de Ven W.J.M., Kas K.
      Cancer Res. 60:106-113(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DNA-BINDING, MUTAGENESIS OF HIS-92 AND HIS-227.
    10. "PLAG1 fusion oncogenes in lipoblastoma."
      Hibbard M.K., Kozakewich H.P., Dal Cin P., Sciot R., Tan X., Xiao S., Fletcher J.A.
      Cancer Res. 60:4869-4872(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHROMOSOMAL REARRANGEMENT WITH HAS2 AND COL1A2.
    11. "The tumorigenic diversity of the three PLAG family members is associated with different DNA binding capacities."
      Hensen K., Van Valckenborgh I.C.C., Kas K., Van de Ven W.J.M., Voz M.L.
      Cancer Res. 62:1510-1517(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF HIS-227.
    12. "Identification of a karyopherin alpha 2 recognition site in PLAG1, which functions as a nuclear localization signal."
      Braem C.V., Kas K., Meyen E., Debiec-Rychter M., Van De Ven W.J.M., Voz M.L.
      J. Biol. Chem. 277:19673-19678(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KPNA2, SUBCELLULAR LOCATION, MUTAGENESIS OF 23-ARG-LYS-24 AND 31-ARG-LYS-32.
    13. Cited for: FUNCTION, TISSUE SPECIFICITY.
    14. "Repression of the transactivating capacity of the oncoprotein PLAG1 by SUMOylation."
      Van Dyck F., Delvaux E.L.D., Van de Ven W.J.M., Chavez M.V.
      J. Biol. Chem. 279:36121-36131(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION, MUTAGENESIS OF LYS-244; LYS-263 AND LYS-353, INTERACTION WITH PIAS PROTEINS.
    15. Cited for: FUNCTION.
    16. "PLAG1-HAS2 fusion in lipoblastoma with masked 8q intrachromosomal rearrangement."
      Morerio C., Rapella A., Rosanda C., Tassano E., Gambini C., Romagnoli G., Panarello C.
      Cancer Genet. Cytogenet. 156:183-184(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHROMOSOMAL REARRANGEMENT WITH HAS2.
    17. "Sumoylation and acetylation play opposite roles in the transactivation of PLAG1 and PLAGL2."
      Zheng G., Yang Y.-C.
      J. Biol. Chem. 280:40773-40781(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUMOYLATION, ACETYLATION, MUTAGENESIS OF LYS-244; LYS-263 AND LYS-353, SUBCELLULAR LOCATION.
    18. "CHCHD7-PLAG1 and TCEA1-PLAG1 gene fusions resulting from cryptic, intrachromosomal 8q rearrangements in pleomorphic salivary gland adenomas."
      Asp J., Persson F., Kost-Alimova M., Stenman G.
      Genes Chromosomes Cancer 45:820-828(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHROMOSOMAL REARRANGEMENT WITH CHCHD7 AND TCEA1.

    Entry informationi

    Entry nameiPLAG1_HUMAN
    AccessioniPrimary (citable) accession number: Q6DJT9
    Secondary accession number(s): B4DLC2, Q59GH8, Q9Y4L2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Residual nuclear import after mutation of the nuclear localization signal is assigned to zinc finger domains of PLAG1.
    When cultured cells transformed by PLAG1 overexpression are injected in nude mouse, rapidly growing tumors (fibrosarcomaS) are observed at the site of inoculation.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3