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Protein

Polypeptide N-acetylgalactosaminyltransferase 11

Gene

galnt11

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Polypeptide N-acetylgalactosaminyltransferase that catalyzes the initiation of protein O-linked glycosylation and is involved in left/right asymmetry by mediating O-glycosylation of NOTCH1. O-glycosylation of NOTCH1 promotes activation of NOTCH1, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Protein has several cofactor binding sites:

Pathway:iprotein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • Notch binding Source: UniProtKB
  • polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB

GO - Biological processi

  • cilium morphogenesis Source: UniProtKB
  • determination of left/right symmetry Source: UniProtKB
  • Notch receptor processing Source: UniProtKB
  • Notch signaling involved in heart development Source: UniProtKB
  • protein O-linked glycosylation via threonine Source: UniProtKB
  • regulation of Notch signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Notch signaling pathway

Keywords - Ligandi

Calcium, Lectin, Manganese

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 11 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 11
Short name:
GalNAc-T11
Short name:
pp-GaNTase 11
Protein-UDP acetylgalactosaminyltransferase 11
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11
Gene namesi
Name:galnt11
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-981830. galnt11.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 601573LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Aberrant left-right patterning resulting in abnormal cardiac loops, including leftward and symmetric/midline loops.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601Polypeptide N-acetylgalactosaminyltransferase 11PRO_0000425208Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi486 ↔ 505PROSITE-ProRule annotation
Glycosylationi508 – 5081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi529 ↔ 546PROSITE-ProRule annotation
Disulfide bondi571 ↔ 589PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Interacts with notch1.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini469 – 600132Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni143 – 254112Catalytic subdomain ABy similarityAdd
BLAST
Regioni312 – 37463Catalytic subdomain BBy similarityAdd
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG051699.
KOiK00710.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR027791. Galactosyl_T_C.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DJR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSAALRCFC YGCLFTSVTW TLLLFIYFNF SEESQGFRHV PVKGLEPYKP
60 70 80 90 100
LPKKIYPRFS RDSMGQHSDP RKGHNGNQLE TEANADLSPE LGMIFNEQDQ
110 120 130 140 150
DVRDVGYQKH AFNLLISNRL GYHRDVPDTR DSKCAKKTYP PDLPMASIVI
160 170 180 190 200
CFYNEAFSAL LRTVHSVLDR TPAQLLHEII LVDDNSELDD LKKDLDGYMQ
210 220 230 240 250
ENLSKKVKLV RNKQREGLIR GRMVGASHAT GDVLVFLDSH CEVNEMWLQP
260 270 280 290 300
LLAPIKENPR TVVCPVIDII SADTLIYSSS PVVRGGFNWG LHFKWDPVPL
310 320 330 340 350
AELGGPEGFS APFRSPTMAG GLFAMDREYF NMLGQYDSGM DIWGGENLEI
360 370 380 390 400
SFRIWMCGGS LLIVPCSRVG HIFRKRRPYG SPGGHDTMAH NSLRLAHVWM
410 420 430 440 450
DEYKDQYFAL RPELRNRDFG DIRERLALRR RLNCKSFKWY LDNIYPEMQV
460 470 480 490 500
SGPNAKPQPP VFMNKGQKRP KILQRGRLIN MQTNRCLVAQ GHPSQKGGLV
510 520 530 540 550
VAKECDYNDS EQVWSYNEEH ELILSNLLCL DMSETRSSDP PRLMKCHGSG
560 570 580 590 600
GSQQWVFGKS NRLYQVSVGQ CLKLVDPMSR KGYVSMAICD GSPSQQWHLE

N
Length:601
Mass (Da):68,383
Last modified:January 22, 2014 - v2
Checksum:i1FA0294A3CEEA236
GO

Sequence cautioni

The sequence AAH75106.1 differs from that shown. Reason: Frameshift at position 39. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC075106 mRNA. Translation: AAH75106.1. Frameshift.
RefSeqiNP_001006904.1. NM_001006903.1.
UniGeneiStr.1760.

Genome annotation databases

GeneIDi448751.
KEGGixtr:448751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC075106 mRNA. Translation: AAH75106.1. Frameshift.
RefSeqiNP_001006904.1. NM_001006903.1.
UniGeneiStr.1760.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi448751.
KEGGixtr:448751.

Organism-specific databases

CTDi63917.
XenbaseiXB-GENE-981830. galnt11.

Phylogenomic databases

HOVERGENiHBG051699.
KOiK00710.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR027791. Galactosyl_T_C.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  2. "The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality."
    Boskovski M.T., Yuan S., Pedersen N.B., Goth C.K., Makova S., Clausen H., Brueckner M., Khokha M.K.
    Nature 504:456-459(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH NOTCH1.

Entry informationi

Entry nameiGLT11_XENTR
AccessioniPrimary (citable) accession number: Q6DJR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: January 22, 2014
Last modified: May 27, 2015
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.