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Q6DJR8

- GLT11_XENTR

UniProt

Q6DJR8 - GLT11_XENTR

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Protein

Polypeptide N-acetylgalactosaminyltransferase 11

Gene
galnt11
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Polypeptide N-acetylgalactosaminyltransferase that catalyzes the initiation of protein O-linked glycosylation and is involved in left/right asymmetry by mediating O-glycosylation of NOTCH1. O-glycosylation of NOTCH1 promotes activation of NOTCH1, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Manganese By similarity.
Calcium By similarity.

Pathwayi

GO - Molecular functioni

  1. Notch binding Source: UniProtKB
  2. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB

GO - Biological processi

  1. cilium morphogenesis Source: UniProtKB
  2. determination of left/right symmetry Source: UniProtKB
  3. Notch receptor processing Source: UniProtKB
  4. Notch signaling involved in heart development Source: UniProtKB
  5. protein O-linked glycosylation via threonine Source: UniProtKB
  6. regulation of Notch signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Notch signaling pathway

Keywords - Ligandi

Calcium, Lectin, Manganese

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Polypeptide N-acetylgalactosaminyltransferase 11 (EC:2.4.1.41)
Alternative name(s):
Polypeptide GalNAc transferase 11
Short name:
GalNAc-T11
Short name:
pp-GaNTase 11
Protein-UDP acetylgalactosaminyltransferase 11
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11
Gene namesi
Name:galnt11
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-981830. galnt11.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77Cytoplasmic Reviewed prediction
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini29 – 601573Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Aberrant left-right patterning resulting in abnormal cardiac loops, including leftward and symmetric/midline loops.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601Polypeptide N-acetylgalactosaminyltransferase 11PRO_0000425208Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi202 – 2021N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi486 ↔ 505 By similarity
Glycosylationi508 – 5081N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi529 ↔ 546 By similarity
Disulfide bondi571 ↔ 589 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Interacts with notch1.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini469 – 600132Ricin B-type lectinAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni143 – 254112Catalytic subdomain A By similarityAdd
BLAST
Regioni312 – 37463Catalytic subdomain B By similarityAdd
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG051699.
KOiK00710.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR027791. Galactosyl_T_C.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DJR8-1 [UniParc]FASTAAdd to Basket

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MGSAALRCFC YGCLFTSVTW TLLLFIYFNF SEESQGFRHV PVKGLEPYKP    50
LPKKIYPRFS RDSMGQHSDP RKGHNGNQLE TEANADLSPE LGMIFNEQDQ 100
DVRDVGYQKH AFNLLISNRL GYHRDVPDTR DSKCAKKTYP PDLPMASIVI 150
CFYNEAFSAL LRTVHSVLDR TPAQLLHEII LVDDNSELDD LKKDLDGYMQ 200
ENLSKKVKLV RNKQREGLIR GRMVGASHAT GDVLVFLDSH CEVNEMWLQP 250
LLAPIKENPR TVVCPVIDII SADTLIYSSS PVVRGGFNWG LHFKWDPVPL 300
AELGGPEGFS APFRSPTMAG GLFAMDREYF NMLGQYDSGM DIWGGENLEI 350
SFRIWMCGGS LLIVPCSRVG HIFRKRRPYG SPGGHDTMAH NSLRLAHVWM 400
DEYKDQYFAL RPELRNRDFG DIRERLALRR RLNCKSFKWY LDNIYPEMQV 450
SGPNAKPQPP VFMNKGQKRP KILQRGRLIN MQTNRCLVAQ GHPSQKGGLV 500
VAKECDYNDS EQVWSYNEEH ELILSNLLCL DMSETRSSDP PRLMKCHGSG 550
GSQQWVFGKS NRLYQVSVGQ CLKLVDPMSR KGYVSMAICD GSPSQQWHLE 600
N 601
Length:601
Mass (Da):68,383
Last modified:January 22, 2014 - v2
Checksum:i1FA0294A3CEEA236
GO

Sequence cautioni

The sequence AAH75106.1 differs from that shown. Reason: Frameshift at position 39.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC075106 mRNA. Translation: AAH75106.1. Frameshift.
RefSeqiNP_001006904.1. NM_001006903.1.
UniGeneiStr.1760.

Genome annotation databases

GeneIDi448751.
KEGGixtr:448751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC075106 mRNA. Translation: AAH75106.1 . Frameshift.
RefSeqi NP_001006904.1. NM_001006903.1.
UniGenei Str.1760.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 448751.
KEGGi xtr:448751.

Organism-specific databases

CTDi 63917.
Xenbasei XB-GENE-981830. galnt11.

Phylogenomic databases

HOVERGENi HBG051699.
KOi K00710.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR027791. Galactosyl_T_C.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  2. "The heterotaxy gene GALNT11 glycosylates Notch to orchestrate cilia type and laterality."
    Boskovski M.T., Yuan S., Pedersen N.B., Goth C.K., Makova S., Clausen H., Brueckner M., Khokha M.K.
    Nature 504:456-459(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH NOTCH1.

Entry informationi

Entry nameiGLT11_XENTR
AccessioniPrimary (citable) accession number: Q6DJR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: January 22, 2014
Last modified: July 9, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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