Reviewed, UniProtKB/Swiss-Prot Q6DIY9 (MDHC_XENTR)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase, cytoplasmic
    EC=1.1.1.37
Alternative name(s):
    Cytosolic malate dehydrogenase

Gene names

Name:	mdh1
ORF Names:	TEgg049g04.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Malate dehydrogenase, cytoplasmic		PRO_0000226740
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	129 – 131	3	NAD By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	NAD By similarity
	Binding site	112	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DIY9-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 3771224ABFDCF324	FASTA	334	36,543
	10 20 30 40 50 60 MPEPVKVLVT GAAGQIAYSL LYGIAKGDVF GKDQPLILVL LDITPMMTVL EGVVMELQDC 70 80 90 100 110 120 ALPLLKEVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALNKYS 130 140 150 160 170 180 KKSVKVIVVG NPANTNCLTA MKSAPSIPKE NFSCLTRLDH NRAKGQIALK LNVASDDVKN 190 200 210 220 230 240 VIIWGNHSST QYPDASHATV NLQGKDVGAF EAVKNDDWLK GEFITTVQQR GAAVIKARKL 250 260 270 280 290 300 SSAMSAAKAI CDHVRDIWFG TPDGQFVSMG VISDGNSYGI PEDLMYSFPL TIKNKTWKIV 310 320 330 EGLPINDFSR EKMDLTAKEL HEEKETALEF LSCE

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR761081 mRNA. Translation: CAJ81507.1. BC075396 mRNA. Translation: AAH75396.1.
RefSeq	NP_001006694.1.
UniGene	Str.84
3D structure databases
SMR	Q6DIY9. Positions 3-332.
ModBase	Search...
Genome annotation databases
GeneID	448318.
KEGG	xtr:448318.
Organism-specific databases
Xenbase	XB-FEAT-484871. mdh1.
Phylogenomic databases
HOVERGEN	Q6DIY9.
Enzyme and pathway databases
BRENDA	1.1.1.37. 279072.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR011274. Malate_DH_NAD-dep_euk. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit. TIGR01758. MDH_euk_cyt. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHC_XENTR

Accession

Primary (citable) accession number: Q6DIY9
Secondary accession number(s): Q28H34

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	August 16, 2004
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project