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Protein

Ubiquitin carboxyl-terminal hydrolase 10

Gene

usp10

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/tp53. Acts as an essential regulator of p53/tp53 stability: in unstressed cells, specifically deubiquitinates p53/tp53 in the cytoplasm, leading to counteracts MDM2 action and stabilize p53/tp53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/tp53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/tp53 levels (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei431 – 4311NucleophilePROSITE-ProRule annotation
Active sitei756 – 7561Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ion channel binding Source: UniProtKB
  3. p53 binding Source: UniProtKB
  4. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  3. DNA repair Source: UniProtKB-KW
  4. protein deubiquitination Source: UniProtKB
  5. regulation of autophagy Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Autophagy, DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 10 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 10
Ubiquitin thioesterase 10
Ubiquitin-specific-processing protease 10
Gene namesi
Name:usp10
ORF Names:TGas137m11.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unplaced

Organism-specific databases

XenbaseiXB-GENE-966184. usp10.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. early endosome Source: UniProtKB
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Ubiquitin carboxyl-terminal hydrolase 10PRO_0000393004Add
BLAST

Proteomic databases

PaxDbiQ6DIJ4.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000029355.

Structurei

3D structure databases

ProteinModelPortaliQ6DIJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini422 – 802381USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP10 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000285959.
HOVERGENiHBG059823.
InParanoidiQ6DIJ4.
KOiK11841.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR028767. USP10.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PANTHERiPTHR24006:SF69. PTHR24006:SF69. 1 hit.
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DIJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPSGQYIF GEFSDDEFKQ FFVTARCTVE LPPYNEHFFP CGPQSSGDFQ
60 70 80 90 100
DDMHLKFSEV IHGISGEDCP RIEFGIEEVI DHSTALPNNT DYSISSNLNP
110 120 130 140 150
QAPEFILTCS SSPKTSNNVL HENNFDAINC QFSECAIPDG SGNADSDGTS
160 170 180 190 200
GTGQRERKKK KKRPPGYYSY LEGVGDVPSE TLVNGHANST GLNSISTDDP
210 220 230 240 250
DLAEDIPIST TSPRTCNSPD NFVDLNNEAL SNDASMHNAL DNARTAGQPE
260 270 280 290 300
ECSVTSSEQS CIPSDNGRES PVRTAVVQPF AGTDTTENLG VTNGQTLESS
310 320 330 340 350
EEGTASNGVV LLPEVSSLSE EAKPEETSTA QAVVHLPGSA SANPPAKSWA
360 370 380 390 400
SLFHNSKPSS TTQVAYVETK NATPVTSPQV TEKQVEIKEG PVPVSEDPVA
410 420 430 440 450
IKIAELLEEV KLVHKPVSLQ PRGLINKGNW CYINATLQAL VACPPMYHLM
460 470 480 490 500
KSIPVYTKAQ RPCTSTPMID SFVRLMNEFT NMPILPKAKQ ASGEKVIRDI
510 520 530 540 550
RPGAPFEPAY IYRLLTVFKS SLSEKGRQED AEEYLGFILN GLHEEMLSLK
560 570 580 590 600
KLLLPQNDKI HINNGPDPVS EKEEINKDEQ EGSDEEWEQV GPRHKSSVTR
610 620 630 640 650
QADFVQTPIT DIFGGHMRSV VYQQSSKESA TLQPFFTLQL DIQSEKIRTV
660 670 680 690 700
QDALESLVAR ESVQGYTTKT KQEVEICRRV TLEELPPVLV LHLKRFVFEK
710 720 730 740 750
TGGCQKLIKN IEYPVDLEIS KDLLSPGVKS KIFKGQRTYR LFAVVYHHGN
760 770 780 790 800
SATGGHYTTD VFQIGLNGWL RIDDQTVKVI NQYQVVKQTV ERTAYLLYYR

RVDLL
Length:805
Mass (Da):88,936
Last modified:August 16, 2004 - v1
Checksum:iF95A8A2A4FB60DD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR855702 mRNA. Translation: CAJ83514.1.
BC075544 mRNA. Translation: AAH75544.1.
RefSeqiNP_001006761.1. NM_001006760.1.
UniGeneiStr.51.

Genome annotation databases

GeneIDi448441.
KEGGixtr:448441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR855702 mRNA. Translation: CAJ83514.1.
BC075544 mRNA. Translation: AAH75544.1.
RefSeqiNP_001006761.1. NM_001006760.1.
UniGeneiStr.51.

3D structure databases

ProteinModelPortaliQ6DIJ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000029355.

Protein family/group databases

MEROPSiC19.018.

Proteomic databases

PaxDbiQ6DIJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi448441.
KEGGixtr:448441.

Organism-specific databases

CTDi9100.
XenbaseiXB-GENE-966184. usp10.

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000285959.
HOVERGENiHBG059823.
InParanoidiQ6DIJ4.
KOiK11841.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR028767. USP10.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PANTHERiPTHR24006:SF69. PTHR24006:SF69. 1 hit.
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Sanger Xenopus tropicalis EST/cDNA project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Gastrula.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiUBP10_XENTR
AccessioniPrimary (citable) accession number: Q6DIJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: August 16, 2004
Last modified: March 4, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.