Q6DIJ4 (UBP10_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 10
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 10
Ubiquitin thioesterase 10
Ubiquitin-specific-processing protease 10

Gene names

Name:	usp10
ORF Names:	TGas137m11.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	805 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/tp53. Acts as an essential regulator of p53/tp53 stability: in unstressed cells, specifically deubiquitinates p53/tp53 in the cytoplasm, leading to counteracts MDM2 action and stabilize p53/tp53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/tp53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/tp53 levels By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP10 subfamily.

Ontologies

Keywords
Biological process	Autophagy DNA damage DNA repair Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage response, signal transduction by p53 class mediator Inferred from sequence or structural similarity. Source: UniProtKB DNA repair Inferred from electronic annotation. Source: UniProtKB-KW autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB regulation of autophagy Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	early endosome Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB ion channel binding Inferred from sequence or structural similarity. Source: UniProtKB p53 binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 805	805	Ubiquitin carboxyl-terminal hydrolase 10		PRO_0000393004
Sites
	Active site	431	1	Nucleophile By similarity
	Active site	756	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DIJ4 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: F95A8A2A4FB60DD1	FASTA	805	88,936
	10 20 30 40 50 60 MASPSGQYIF GEFSDDEFKQ FFVTARCTVE LPPYNEHFFP CGPQSSGDFQ DDMHLKFSEV 70 80 90 100 110 120 IHGISGEDCP RIEFGIEEVI DHSTALPNNT DYSISSNLNP QAPEFILTCS SSPKTSNNVL 130 140 150 160 170 180 HENNFDAINC QFSECAIPDG SGNADSDGTS GTGQRERKKK KKRPPGYYSY LEGVGDVPSE 190 200 210 220 230 240 TLVNGHANST GLNSISTDDP DLAEDIPIST TSPRTCNSPD NFVDLNNEAL SNDASMHNAL 250 260 270 280 290 300 DNARTAGQPE ECSVTSSEQS CIPSDNGRES PVRTAVVQPF AGTDTTENLG VTNGQTLESS 310 320 330 340 350 360 EEGTASNGVV LLPEVSSLSE EAKPEETSTA QAVVHLPGSA SANPPAKSWA SLFHNSKPSS 370 380 390 400 410 420 TTQVAYVETK NATPVTSPQV TEKQVEIKEG PVPVSEDPVA IKIAELLEEV KLVHKPVSLQ 430 440 450 460 470 480 PRGLINKGNW CYINATLQAL VACPPMYHLM KSIPVYTKAQ RPCTSTPMID SFVRLMNEFT 490 500 510 520 530 540 NMPILPKAKQ ASGEKVIRDI RPGAPFEPAY IYRLLTVFKS SLSEKGRQED AEEYLGFILN 550 560 570 580 590 600 GLHEEMLSLK KLLLPQNDKI HINNGPDPVS EKEEINKDEQ EGSDEEWEQV GPRHKSSVTR 610 620 630 640 650 660 QADFVQTPIT DIFGGHMRSV VYQQSSKESA TLQPFFTLQL DIQSEKIRTV QDALESLVAR 670 680 690 700 710 720 ESVQGYTTKT KQEVEICRRV TLEELPPVLV LHLKRFVFEK TGGCQKLIKN IEYPVDLEIS 730 740 750 760 770 780 KDLLSPGVKS KIFKGQRTYR LFAVVYHHGN SATGGHYTTD VFQIGLNGWL RIDDQTVKVI 790 800 NQYQVVKQTV ERTAYLLYYR RVDLL

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Gastrula.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR855702 mRNA. Translation: CAJ83514.1. BC075544 mRNA. Translation: AAH75544.1.
RefSeq	NP_001006761.1. NM_001006760.1.
UniGene	Str.51.
3D structure databases
ProteinModelPortal	Q6DIJ4.
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000029355.
Protein family/group databases
MEROPS	C19.018.
Proteomic databases
PaxDb	Q6DIJ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	448441.
KEGG	xtr:448441.
Organism-specific databases
CTD	9100.
Xenbase	XB-GENE-966184. usp10.
Phylogenomic databases
eggNOG	COG5533.
HOGENOM	HOG000285959.
HOVERGEN	HBG059823.
InParanoid	Q6DIJ4.
KO	K11841.
OrthoDB	EOG4FN4H7.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP10_XENTR

Accession

Primary (citable) accession number: Q6DIJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	August 16, 2004
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program