Reviewed, UniProtKB/Swiss-Prot Q6DIJ1 (SYYC_XENTR)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.1
Alternative name(s):
    Tyrosyl--tRNA ligase
      Short name=TyrRS

Gene names

Name:

yars

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Tyrosyl-tRNA synthetase, cytoplasmic		PRO_0000239695
Regions
	Domain	364 – 468	105	tRNA-binding
	Motif	44 – 52	9	"HIGH" region By similarity
	Motif	222 – 226	5	"KMSKS" region By similarity
Sites
	Binding site	39	1	Tyrosine By similarity
	Binding site	166	1	Tyrosine By similarity
	Binding site	170	1	Tyrosine By similarity
	Binding site	173	1	Tyrosine By similarity
	Binding site	188	1	Tyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DIJ1-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 982F25E6AE4AB9E5	FASTA	528	58,958
	10 20 30 40 50 60 MGDGLTPEGK AQLITRNLQE VLGEDKMKEI LKERPLRIYW GTATTGKPHV AYFVPMSKIA 70 80 90 100 110 120 DFLKAGCEVT ILFADLHAYL DNMKAPWDLL ELRTRYYEQV IQAMLQSIGV PLERLRFIRG 130 140 150 160 170 180 TEFQLSKEYT LDVYRLSSVV TQHDAKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD 190 200 210 220 230 240 AQFGGVDQRK IFTFAEKYLP ALGYAKRIHL MNPMVPGLTG AKMSSSEEES KIDLLDSPAD 250 260 270 280 290 300 VKKKLKKAFC EPGNIENNGV LSFVRHVLFP LKSEFVVLRD EKFGGNKTYT DFETLEKDFT 310 320 330 340 350 360 EQLVHPGDLK ASVEKALNKL LDPIREKFNS PEMKKLSNDA YPGASKQKTV PKGSTKNSGP 370 380 390 400 410 420 EEIDPSLLDL RVGKILSVRQ HPDADSLYVE SVDVGEENPR CVVSGLVQYV PSDQLLGRSV 430 440 450 460 470 480 VLLCNLKPQK MRGIESQGML LCASTEGEQK QVEPLDPPTG SAPGERIYIE GYENGEPEGE 490 500 510 520 LKPKKKVFEK LQVDFRISDD LCAQWKGKNF LTKLGSVTCK TLRGGSIS

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC075547 mRNA. Translation: AAH75547.1.
RefSeq	NP_001004987.1.
UniGene	Str.4835
3D structure databases
SMR	Q6DIJ1. Positions 6-342, 360-528.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6DIJ1.
Genome annotation databases
Ensembl	ENSXETT00000057912; ENSXETP00000057912; ENSXETG00000027865; Xenopus tropicalis. [Genome view]
GeneID	448444.
KEGG	xtr:448444.
Organism-specific databases
CTD	448444.
Xenbase	XB-FEAT-491607. yars.
Phylogenomic databases
eggNOG	veNOG07402.
HOGENOM	HBG330667.
HOVERGEN	Q6DIJ1.
InParanoid	Q6DIJ1.
Enzyme and pathway databases
BRENDA	6.1.1.1. 279072.
Gene expression databases
Bgee	Q6DIJ1.
Family and domain databases
InterPro	IPR002305. aa-tRNA-synth_Ib. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR015624. Tyr-tRNA-synth_Ib_arc/euk. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF8. Tyr-tRNA_synth. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYYC_XENTR

Accession

Primary (citable) accession number: Q6DIJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project