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Q6DID7

- WLS_MOUSE

UniProt

Q6DID7 - WLS_MOUSE

Protein

Protein wntless homolog

Gene

Wls

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. Wnt-protein binding Source: UniProtKB

    GO - Biological processi

    1. anterior/posterior axis specification Source: UniProtKB
    2. mesoderm formation Source: UniProtKB
    3. positive regulation of canonical Wnt signaling pathway Source: Ensembl
    4. Wnt signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_213918. WNT ligand biogenesis and trafficking.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein wntless homolog
    Alternative name(s):
    Integral membrane protein GPR177
    Protein evenness interrupted homolog
    Short name:
    EVI
    Gene namesi
    Name:Wls
    Synonyms:Gpr177
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1915401. Wls.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: UniProtKB
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. Golgi apparatus Source: UniProtKB
    4. Golgi membrane Source: UniProtKB-SubCell
    5. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice display embryonic lethality before E10.5. Embryos show defects in the establishment of the body axis and in the primitive streak and mesoderm formation.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4242Sequence AnalysisAdd
    BLAST
    Chaini43 – 541499Protein wntless homologPRO_0000271779Add
    BLAST

    Proteomic databases

    MaxQBiQ6DID7.
    PaxDbiQ6DID7.
    PRIDEiQ6DID7.

    PTM databases

    PhosphoSiteiQ6DID7.

    Expressioni

    Tissue specificityi

    Expressed in kidney, lung, skin, intestine, brain, spinal cord, skeleton, eyes, excretion glands, tooth and palatal shelves.1 Publication

    Developmental stagei

    Detected at E6.25 in the proximal epiblast at the junction between the embryonic and extraembryonic tissue. Later expression is more restricted to the primitive streak and mesoderm extending to the distal tip of the embryo. Strong expression is found in both posterior visceral endoderm and epiblast at the prestreak, but switched to the mesoderm at late-streak.1 Publication

    Inductioni

    Up-regulated by WNT1. Transcriptionally activated by beta-catenin and by LEF/TCF-dependent transcription.1 Publication

    Gene expression databases

    BgeeiQ6DID7.
    CleanExiMM_GPR177.
    GenevestigatoriQ6DID7.

    Interactioni

    Subunit structurei

    Interacts with WNT3A By similarity. Interacts with WNT1, WNT3 and WNT5.By similarity1 Publication

    Protein-protein interaction databases

    DIPiDIP-49004N.
    IntActiQ6DID7. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6DID7.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini43 – 232190LumenalSequence AnalysisAdd
    BLAST
    Topological domaini254 – 26815CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini290 – 30314LumenalSequence AnalysisAdd
    BLAST
    Topological domaini325 – 3317CytoplasmicSequence Analysis
    Topological domaini353 – 38028LumenalSequence AnalysisAdd
    BLAST
    Topological domaini402 – 43130CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini453 – 47119LumenalSequence AnalysisAdd
    BLAST
    Topological domaini493 – 54149CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei233 – 25321Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei269 – 28921Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei304 – 32421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei332 – 35221Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei381 – 40121Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei432 – 45221Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei472 – 49221Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni101 – 232132Interacts with Wnt proteinsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the wntless family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG77798.
    GeneTreeiENSGT00390000005897.
    HOGENOMiHOG000039226.
    HOVERGENiHBG055794.
    InParanoidiQ6DID7.
    OMAiKEMSPWF.
    OrthoDBiEOG7B05CP.
    PhylomeDBiQ6DID7.
    TreeFamiTF105975.

    Family and domain databases

    InterProiIPR009551. Wntless.
    [Graphical view]
    PANTHERiPTHR13449. PTHR13449. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q6DID7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGAIIENMS TKKLCIVGGI LLVFQIVAFL VGGLIAPAPT TAVPYTAIKC    50
    VDVRKNHHKT RWLAPWGPNK CDKIRDIEEA IPREIEANDI VFSVHIPLPS 100
    MEMSPWFQFM LFILQLDIAF KLNNQIRENA EISMDVSLGY RDDMFSEWTE 150
    MAHERVPRKL KCTFTSPKTP EHEGRYYNCD VLPFMEIGSV AHKYYLLNIR 200
    LPVNEKKKIN VGIGEIKDIR LVGIHQNGGF TKVWFAMKTF LTPSIFIIMV 250
    WYWRRITMMS RPPVLLEKVI FALGISMTFI NIPVEWFSIG FDWTWMLLFG 300
    DIRQGIFYAM LLSFWIIFCG EHMMDQHERN HIAGYWKQVG PIAVGSFCLF 350
    IFDMCERGVQ LTNPFYSIWT TDVGTELAMA FIIVAGICLC LYFLFLCFMV 400
    FQVFRNISGK QSSLPAMSKV RRLHYEGLIF RFKFLMLITL ACAAMTVIFF 450
    IVSQVSEGHW KWGGVTVQVS SAFFTGIYGM WNLYVFALMF LYAPSHKNYG 500
    EDQSNGDLGV HSGEELQLTT TITHVDGPTE IYKLTRKEAQ E 541
    Length:541
    Mass (Da):62,188
    Last modified:August 16, 2004 - v1
    Checksum:i14BDD97D6050D976
    GO
    Isoform 2 (identifier: Q6DID7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-100: Missing.

    Show »
    Length:441
    Mass (Da):51,263
    Checksum:i483373970A7F1877
    GO

    Sequence cautioni

    The sequence AAH18381.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC26266.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC26300.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC32102.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC33950.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti113 – 1131I → V in BAC26266. (PubMed:16141072)Curated
    Sequence conflicti540 – 5401Q → L in BAC26266. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 100100Missing in isoform 2. 1 PublicationVSP_022348Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK019886 mRNA. Translation: BAB31904.1.
    AK029053 mRNA. Translation: BAC26266.1. Different initiation.
    AK029104 mRNA. Translation: BAC26300.1. Different initiation.
    AK044810 mRNA. Translation: BAC32102.1. Different initiation.
    AK049842 mRNA. Translation: BAC33950.1. Different initiation.
    AK077407 mRNA. Translation: BAC36788.1.
    AK159207 mRNA. Translation: BAE34898.1.
    BC075615 mRNA. Translation: AAH75615.1.
    BC018381 mRNA. Translation: AAH18381.1. Different initiation.
    CCDSiCCDS38684.1. [Q6DID7-1]
    RefSeqiNP_080858.3. NM_026582.4. [Q6DID7-1]
    XP_006502027.1. XM_006501964.1. [Q6DID7-1]
    UniGeneiMm.6766.

    Genome annotation databases

    EnsembliENSMUST00000068952; ENSMUSP00000067898; ENSMUSG00000028173. [Q6DID7-1]
    GeneIDi68151.
    KEGGimmu:68151.
    UCSCiuc008rwc.1. mouse. [Q6DID7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK019886 mRNA. Translation: BAB31904.1 .
    AK029053 mRNA. Translation: BAC26266.1 . Different initiation.
    AK029104 mRNA. Translation: BAC26300.1 . Different initiation.
    AK044810 mRNA. Translation: BAC32102.1 . Different initiation.
    AK049842 mRNA. Translation: BAC33950.1 . Different initiation.
    AK077407 mRNA. Translation: BAC36788.1 .
    AK159207 mRNA. Translation: BAE34898.1 .
    BC075615 mRNA. Translation: AAH75615.1 .
    BC018381 mRNA. Translation: AAH18381.1 . Different initiation.
    CCDSi CCDS38684.1. [Q6DID7-1 ]
    RefSeqi NP_080858.3. NM_026582.4. [Q6DID7-1 ]
    XP_006502027.1. XM_006501964.1. [Q6DID7-1 ]
    UniGenei Mm.6766.

    3D structure databases

    ProteinModelPortali Q6DID7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-49004N.
    IntActi Q6DID7. 1 interaction.

    PTM databases

    PhosphoSitei Q6DID7.

    Proteomic databases

    MaxQBi Q6DID7.
    PaxDbi Q6DID7.
    PRIDEi Q6DID7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000068952 ; ENSMUSP00000067898 ; ENSMUSG00000028173 . [Q6DID7-1 ]
    GeneIDi 68151.
    KEGGi mmu:68151.
    UCSCi uc008rwc.1. mouse. [Q6DID7-1 ]

    Organism-specific databases

    CTDi 79971.
    MGIi MGI:1915401. Wls.

    Phylogenomic databases

    eggNOGi NOG77798.
    GeneTreei ENSGT00390000005897.
    HOGENOMi HOG000039226.
    HOVERGENi HBG055794.
    InParanoidi Q6DID7.
    OMAi KEMSPWF.
    OrthoDBi EOG7B05CP.
    PhylomeDBi Q6DID7.
    TreeFami TF105975.

    Enzyme and pathway databases

    Reactomei REACT_213918. WNT ligand biogenesis and trafficking.

    Miscellaneous databases

    NextBioi 326538.
    PROi Q6DID7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q6DID7.
    CleanExi MM_GPR177.
    Genevestigatori Q6DID7.

    Family and domain databases

    InterProi IPR009551. Wntless.
    [Graphical view ]
    PANTHERi PTHR13449. PTHR13449. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Embryo, Head, Hippocampus, Ovary, Skin and Uterus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
      Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
      Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, INTERACTION WITH WNT1; WNT3 AND WNT5A.

    Entry informationi

    Entry nameiWLS_MOUSE
    AccessioniPrimary (citable) accession number: Q6DID7
    Secondary accession number(s): Q8CE42, Q9D2B7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3