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Protein

Protein wntless homolog

Gene

Wls

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.1 Publication

GO - Molecular functioni

  • Wnt-protein binding Source: UniProtKB

GO - Biological processi

  • anterior/posterior axis specification Source: UniProtKB
  • exocrine pancreas development Source: ParkinsonsUK-UCL
  • hindbrain development Source: ParkinsonsUK-UCL
  • intracellular protein transport Source: MGI
  • mesoderm formation Source: UniProtKB
  • midbrain development Source: ParkinsonsUK-UCL
  • positive regulation of canonical Wnt signaling pathway Source: ParkinsonsUK-UCL
  • positive regulation of Wnt protein secretion Source: ParkinsonsUK-UCL
  • positive regulation of Wnt signaling pathway Source: ParkinsonsUK-UCL
  • Wnt protein secretion Source: MGI
  • Wnt signaling pathway Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-3238698 WNT ligand biogenesis and trafficking

Names & Taxonomyi

Protein namesi
Recommended name:
Protein wntless homolog
Alternative name(s):
Integral membrane protein GPR177
Protein evenness interrupted homolog
Short name:
EVI
Gene namesi
Name:Wls
Synonyms:Gpr177
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1915401 Wls

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini43 – 232LumenalSequence analysisAdd BLAST190
Transmembranei233 – 253Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini254 – 268CytoplasmicSequence analysisAdd BLAST15
Transmembranei269 – 289Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini290 – 303LumenalSequence analysisAdd BLAST14
Transmembranei304 – 324Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini325 – 331CytoplasmicSequence analysis7
Transmembranei332 – 352Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini353 – 380LumenalSequence analysisAdd BLAST28
Transmembranei381 – 401Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini402 – 431CytoplasmicSequence analysisAdd BLAST30
Transmembranei432 – 452Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini453 – 471LumenalSequence analysisAdd BLAST19
Transmembranei472 – 492Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini493 – 541CytoplasmicSequence analysisAdd BLAST49

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display embryonic lethality before E10.5. Embryos show defects in the establishment of the body axis and in the primitive streak and mesoderm formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Sequence analysisAdd BLAST42
ChainiPRO_000027177943 – 541Protein wntless homologAdd BLAST499

Proteomic databases

PaxDbiQ6DID7
PeptideAtlasiQ6DID7
PRIDEiQ6DID7

PTM databases

PhosphoSitePlusiQ6DID7

Expressioni

Tissue specificityi

Expressed in kidney, lung, skin, intestine, brain, spinal cord, skeleton, eyes, excretion glands, tooth and palatal shelves.1 Publication

Developmental stagei

Detected at E6.25 in the proximal epiblast at the junction between the embryonic and extraembryonic tissue. Later expression is more restricted to the primitive streak and mesoderm extending to the distal tip of the embryo. Strong expression is found in both posterior visceral endoderm and epiblast at the prestreak, but switched to the mesoderm at late-streak.1 Publication

Inductioni

Up-regulated by WNT1. Transcriptionally activated by beta-catenin and by LEF/TCF-dependent transcription.1 Publication

Gene expression databases

BgeeiENSMUSG00000028173
CleanExiMM_GPR177
ExpressionAtlasiQ6DID7 baseline and differential
GenevisibleiQ6DID7 MM

Interactioni

Subunit structurei

Interacts with WNT3A (By similarity). Interacts with WNT1, WNT3 and WNT5.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Wnt1P044262EBI-15811068,EBI-1570911

GO - Molecular functioni

  • Wnt-protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi212684, 1 interactor
DIPiDIP-49004N
IntActiQ6DID7, 4 interactors
STRINGi10090.ENSMUSP00000067898

Structurei

3D structure databases

ProteinModelPortaliQ6DID7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 232Interaction with Wnt proteinsAdd BLAST132

Sequence similaritiesi

Belongs to the wntless family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG2W Eukaryota
ENOG410XQ06 LUCA
GeneTreeiENSGT00390000005897
HOGENOMiHOG000039226
HOVERGENiHBG055794
InParanoidiQ6DID7
OMAiFTKVWVS
OrthoDBiEOG091G06QO
PhylomeDBiQ6DID7
TreeFamiTF105975

Family and domain databases

InterProiView protein in InterPro
IPR009551 Wntless
PANTHERiPTHR13449 PTHR13449, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6DID7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGAIIENMS TKKLCIVGGI LLVFQIVAFL VGGLIAPAPT TAVPYTAIKC
60 70 80 90 100
VDVRKNHHKT RWLAPWGPNK CDKIRDIEEA IPREIEANDI VFSVHIPLPS
110 120 130 140 150
MEMSPWFQFM LFILQLDIAF KLNNQIRENA EISMDVSLGY RDDMFSEWTE
160 170 180 190 200
MAHERVPRKL KCTFTSPKTP EHEGRYYNCD VLPFMEIGSV AHKYYLLNIR
210 220 230 240 250
LPVNEKKKIN VGIGEIKDIR LVGIHQNGGF TKVWFAMKTF LTPSIFIIMV
260 270 280 290 300
WYWRRITMMS RPPVLLEKVI FALGISMTFI NIPVEWFSIG FDWTWMLLFG
310 320 330 340 350
DIRQGIFYAM LLSFWIIFCG EHMMDQHERN HIAGYWKQVG PIAVGSFCLF
360 370 380 390 400
IFDMCERGVQ LTNPFYSIWT TDVGTELAMA FIIVAGICLC LYFLFLCFMV
410 420 430 440 450
FQVFRNISGK QSSLPAMSKV RRLHYEGLIF RFKFLMLITL ACAAMTVIFF
460 470 480 490 500
IVSQVSEGHW KWGGVTVQVS SAFFTGIYGM WNLYVFALMF LYAPSHKNYG
510 520 530 540
EDQSNGDLGV HSGEELQLTT TITHVDGPTE IYKLTRKEAQ E
Length:541
Mass (Da):62,188
Last modified:August 16, 2004 - v1
Checksum:i14BDD97D6050D976
GO
Isoform 2 (identifier: Q6DID7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:441
Mass (Da):51,263
Checksum:i483373970A7F1877
GO

Sequence cautioni

The sequence AAH18381 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC26266 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC26300 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC32102 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC33950 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113I → V in BAC26266 (PubMed:16141072).Curated1
Sequence conflicti540Q → L in BAC26266 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0223481 – 100Missing in isoform 2. 1 PublicationAdd BLAST100

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK019886 mRNA Translation: BAB31904.1
AK029053 mRNA Translation: BAC26266.1 Different initiation.
AK029104 mRNA Translation: BAC26300.1 Different initiation.
AK044810 mRNA Translation: BAC32102.1 Different initiation.
AK049842 mRNA Translation: BAC33950.1 Different initiation.
AK077407 mRNA Translation: BAC36788.1
AK159207 mRNA Translation: BAE34898.1
BC075615 mRNA Translation: AAH75615.1
BC018381 mRNA Translation: AAH18381.1 Different initiation.
CCDSiCCDS38684.1 [Q6DID7-1]
RefSeqiNP_080858.3, NM_026582.4 [Q6DID7-1]
XP_006502027.1, XM_006501964.2
UniGeneiMm.6766

Genome annotation databases

EnsembliENSMUST00000068952; ENSMUSP00000067898; ENSMUSG00000028173 [Q6DID7-1]
ENSMUST00000198878; ENSMUSP00000143475; ENSMUSG00000028173 [Q6DID7-1]
GeneIDi68151
KEGGimmu:68151
UCSCiuc008rwc.1 mouse [Q6DID7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiWLS_MOUSE
AccessioniPrimary (citable) accession number: Q6DID7
Secondary accession number(s): Q8CE42, Q9D2B7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 16, 2004
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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