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Q6DID7

- WLS_MOUSE

UniProt

Q6DID7 - WLS_MOUSE

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Protein

Protein wntless homolog

Gene

Wls

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.1 Publication

GO - Molecular functioni

  1. Wnt-protein binding Source: UniProtKB

GO - Biological processi

  1. anterior/posterior axis specification Source: UniProtKB
  2. mesoderm formation Source: UniProtKB
  3. positive regulation of canonical Wnt signaling pathway Source: Ensembl
  4. Wnt signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_213918. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein wntless homolog
Alternative name(s):
Integral membrane protein GPR177
Protein evenness interrupted homolog
Short name:
EVI
Gene namesi
Name:Wls
Synonyms:Gpr177
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1915401. Wls.

Subcellular locationi

Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB
  2. Golgi apparatus Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display embryonic lethality before E10.5. Embryos show defects in the establishment of the body axis and in the primitive streak and mesoderm formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4242Sequence AnalysisAdd
BLAST
Chaini43 – 541499Protein wntless homologPRO_0000271779Add
BLAST

Proteomic databases

MaxQBiQ6DID7.
PaxDbiQ6DID7.
PRIDEiQ6DID7.

PTM databases

PhosphoSiteiQ6DID7.

Expressioni

Tissue specificityi

Expressed in kidney, lung, skin, intestine, brain, spinal cord, skeleton, eyes, excretion glands, tooth and palatal shelves.1 Publication

Developmental stagei

Detected at E6.25 in the proximal epiblast at the junction between the embryonic and extraembryonic tissue. Later expression is more restricted to the primitive streak and mesoderm extending to the distal tip of the embryo. Strong expression is found in both posterior visceral endoderm and epiblast at the prestreak, but switched to the mesoderm at late-streak.1 Publication

Inductioni

Up-regulated by WNT1. Transcriptionally activated by beta-catenin and by LEF/TCF-dependent transcription.1 Publication

Gene expression databases

BgeeiQ6DID7.
CleanExiMM_GPR177.
GenevestigatoriQ6DID7.

Interactioni

Subunit structurei

Interacts with WNT3A (By similarity). Interacts with WNT1, WNT3 and WNT5.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-49004N.
IntActiQ6DID7. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ6DID7.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini43 – 232190LumenalSequence AnalysisAdd
BLAST
Topological domaini254 – 26815CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini290 – 30314LumenalSequence AnalysisAdd
BLAST
Topological domaini325 – 3317CytoplasmicSequence Analysis
Topological domaini353 – 38028LumenalSequence AnalysisAdd
BLAST
Topological domaini402 – 43130CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini453 – 47119LumenalSequence AnalysisAdd
BLAST
Topological domaini493 – 54149CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei233 – 25321Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei269 – 28921Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei304 – 32421Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei332 – 35221Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei381 – 40121Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei432 – 45221Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei472 – 49221Helical; Name=7Sequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 232132Interacts with Wnt proteinsAdd
BLAST

Sequence similaritiesi

Belongs to the wntless family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG77798.
GeneTreeiENSGT00390000005897.
HOGENOMiHOG000039226.
HOVERGENiHBG055794.
InParanoidiQ6DID7.
OMAiKEMSPWF.
OrthoDBiEOG7B05CP.
PhylomeDBiQ6DID7.
TreeFamiTF105975.

Family and domain databases

InterProiIPR009551. Wntless.
[Graphical view]
PANTHERiPTHR13449. PTHR13449. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6DID7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGAIIENMS TKKLCIVGGI LLVFQIVAFL VGGLIAPAPT TAVPYTAIKC
60 70 80 90 100
VDVRKNHHKT RWLAPWGPNK CDKIRDIEEA IPREIEANDI VFSVHIPLPS
110 120 130 140 150
MEMSPWFQFM LFILQLDIAF KLNNQIRENA EISMDVSLGY RDDMFSEWTE
160 170 180 190 200
MAHERVPRKL KCTFTSPKTP EHEGRYYNCD VLPFMEIGSV AHKYYLLNIR
210 220 230 240 250
LPVNEKKKIN VGIGEIKDIR LVGIHQNGGF TKVWFAMKTF LTPSIFIIMV
260 270 280 290 300
WYWRRITMMS RPPVLLEKVI FALGISMTFI NIPVEWFSIG FDWTWMLLFG
310 320 330 340 350
DIRQGIFYAM LLSFWIIFCG EHMMDQHERN HIAGYWKQVG PIAVGSFCLF
360 370 380 390 400
IFDMCERGVQ LTNPFYSIWT TDVGTELAMA FIIVAGICLC LYFLFLCFMV
410 420 430 440 450
FQVFRNISGK QSSLPAMSKV RRLHYEGLIF RFKFLMLITL ACAAMTVIFF
460 470 480 490 500
IVSQVSEGHW KWGGVTVQVS SAFFTGIYGM WNLYVFALMF LYAPSHKNYG
510 520 530 540
EDQSNGDLGV HSGEELQLTT TITHVDGPTE IYKLTRKEAQ E
Length:541
Mass (Da):62,188
Last modified:August 16, 2004 - v1
Checksum:i14BDD97D6050D976
GO
Isoform 2 (identifier: Q6DID7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:441
Mass (Da):51,263
Checksum:i483373970A7F1877
GO

Sequence cautioni

The sequence AAH18381.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC26266.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC26300.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC32102.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC33950.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131I → V in BAC26266. (PubMed:16141072)Curated
Sequence conflicti540 – 5401Q → L in BAC26266. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 100100Missing in isoform 2. 1 PublicationVSP_022348Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK019886 mRNA. Translation: BAB31904.1.
AK029053 mRNA. Translation: BAC26266.1. Different initiation.
AK029104 mRNA. Translation: BAC26300.1. Different initiation.
AK044810 mRNA. Translation: BAC32102.1. Different initiation.
AK049842 mRNA. Translation: BAC33950.1. Different initiation.
AK077407 mRNA. Translation: BAC36788.1.
AK159207 mRNA. Translation: BAE34898.1.
BC075615 mRNA. Translation: AAH75615.1.
BC018381 mRNA. Translation: AAH18381.1. Different initiation.
CCDSiCCDS38684.1. [Q6DID7-1]
RefSeqiNP_080858.3. NM_026582.4. [Q6DID7-1]
XP_006502027.1. XM_006501964.1. [Q6DID7-1]
UniGeneiMm.6766.

Genome annotation databases

EnsembliENSMUST00000068952; ENSMUSP00000067898; ENSMUSG00000028173. [Q6DID7-1]
GeneIDi68151.
KEGGimmu:68151.
UCSCiuc008rwc.1. mouse. [Q6DID7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK019886 mRNA. Translation: BAB31904.1 .
AK029053 mRNA. Translation: BAC26266.1 . Different initiation.
AK029104 mRNA. Translation: BAC26300.1 . Different initiation.
AK044810 mRNA. Translation: BAC32102.1 . Different initiation.
AK049842 mRNA. Translation: BAC33950.1 . Different initiation.
AK077407 mRNA. Translation: BAC36788.1 .
AK159207 mRNA. Translation: BAE34898.1 .
BC075615 mRNA. Translation: AAH75615.1 .
BC018381 mRNA. Translation: AAH18381.1 . Different initiation.
CCDSi CCDS38684.1. [Q6DID7-1 ]
RefSeqi NP_080858.3. NM_026582.4. [Q6DID7-1 ]
XP_006502027.1. XM_006501964.1. [Q6DID7-1 ]
UniGenei Mm.6766.

3D structure databases

ProteinModelPortali Q6DID7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-49004N.
IntActi Q6DID7. 1 interaction.

PTM databases

PhosphoSitei Q6DID7.

Proteomic databases

MaxQBi Q6DID7.
PaxDbi Q6DID7.
PRIDEi Q6DID7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068952 ; ENSMUSP00000067898 ; ENSMUSG00000028173 . [Q6DID7-1 ]
GeneIDi 68151.
KEGGi mmu:68151.
UCSCi uc008rwc.1. mouse. [Q6DID7-1 ]

Organism-specific databases

CTDi 79971.
MGIi MGI:1915401. Wls.

Phylogenomic databases

eggNOGi NOG77798.
GeneTreei ENSGT00390000005897.
HOGENOMi HOG000039226.
HOVERGENi HBG055794.
InParanoidi Q6DID7.
OMAi KEMSPWF.
OrthoDBi EOG7B05CP.
PhylomeDBi Q6DID7.
TreeFami TF105975.

Enzyme and pathway databases

Reactomei REACT_213918. WNT ligand biogenesis and trafficking.

Miscellaneous databases

NextBioi 326538.
PROi Q6DID7.
SOURCEi Search...

Gene expression databases

Bgeei Q6DID7.
CleanExi MM_GPR177.
Genevestigatori Q6DID7.

Family and domain databases

InterProi IPR009551. Wntless.
[Graphical view ]
PANTHERi PTHR13449. PTHR13449. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo, Head, Hippocampus, Ovary, Skin and Uterus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
    Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
    Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, INTERACTION WITH WNT1; WNT3 AND WNT5A.

Entry informationi

Entry nameiWLS_MOUSE
AccessioniPrimary (citable) accession number: Q6DID7
Secondary accession number(s): Q8CE42, Q9D2B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 16, 2004
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3