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Q6DID7 (WLS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein wntless homolog
Alternative name(s):
Integral membrane protein GPR177
Protein evenness interrupted homolog
Short name=EVI
Gene names
Name:Wls
Synonyms:Gpr177
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length541 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development. Ref.3

Subunit structure

Interacts with WNT3A By similarity. Interacts with WNT1, WNT3 and WNT5. Ref.3

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein Ref.3.

Tissue specificity

Expressed in kidney, lung, skin, intestine, brain, spinal cord, skeleton, eyes, excretion glands, tooth and palatal shelves. Ref.3

Developmental stage

Detected at E6.25 in the proximal epiblast at the junction between the embryonic and extraembryonic tissue. Later expression is more restricted to the primitive streak and mesoderm extending to the distal tip of the embryo. Strong expression is found in both posterior visceral endoderm and epiblast at the prestreak, but switched to the mesoderm at late-streak. Ref.3

Induction

Up-regulated by WNT1. Transcriptionally activated by beta-catenin and by LEF/TCF-dependent transcription. Ref.3

Disruption phenotype

Mice display embryonic lethality before E10.5. Embryos show defects in the establishment of the body axis and in the primitive streak and mesoderm formation. Ref.3

Sequence similarities

Belongs to the wntless family.

Sequence caution

The sequence AAH18381.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC26266.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC26300.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC32102.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC33950.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q6DID7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q6DID7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4242 Potential
Chain43 – 541499Protein wntless homolog
PRO_0000271779

Regions

Topological domain43 – 232190Lumenal Potential
Transmembrane233 – 25321Helical; Name=1; Potential
Topological domain254 – 26815Cytoplasmic Potential
Transmembrane269 – 28921Helical; Name=2; Potential
Topological domain290 – 30314Lumenal Potential
Transmembrane304 – 32421Helical; Name=3; Potential
Topological domain325 – 3317Cytoplasmic Potential
Transmembrane332 – 35221Helical; Name=4; Potential
Topological domain353 – 38028Lumenal Potential
Transmembrane381 – 40121Helical; Name=5; Potential
Topological domain402 – 43130Cytoplasmic Potential
Transmembrane432 – 45221Helical; Name=6; Potential
Topological domain453 – 47119Lumenal Potential
Transmembrane472 – 49221Helical; Name=7; Potential
Topological domain493 – 54149Cytoplasmic Potential
Region101 – 232132Interacts with Wnt proteins

Natural variations

Alternative sequence1 – 100100Missing in isoform 2.
VSP_022348

Experimental info

Sequence conflict1131I → V in BAC26266. Ref.1
Sequence conflict5401Q → L in BAC26266. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: 14BDD97D6050D976

FASTA54162,188
        10         20         30         40         50         60 
MAGAIIENMS TKKLCIVGGI LLVFQIVAFL VGGLIAPAPT TAVPYTAIKC VDVRKNHHKT 

        70         80         90        100        110        120 
RWLAPWGPNK CDKIRDIEEA IPREIEANDI VFSVHIPLPS MEMSPWFQFM LFILQLDIAF 

       130        140        150        160        170        180 
KLNNQIRENA EISMDVSLGY RDDMFSEWTE MAHERVPRKL KCTFTSPKTP EHEGRYYNCD 

       190        200        210        220        230        240 
VLPFMEIGSV AHKYYLLNIR LPVNEKKKIN VGIGEIKDIR LVGIHQNGGF TKVWFAMKTF 

       250        260        270        280        290        300 
LTPSIFIIMV WYWRRITMMS RPPVLLEKVI FALGISMTFI NIPVEWFSIG FDWTWMLLFG 

       310        320        330        340        350        360 
DIRQGIFYAM LLSFWIIFCG EHMMDQHERN HIAGYWKQVG PIAVGSFCLF IFDMCERGVQ 

       370        380        390        400        410        420 
LTNPFYSIWT TDVGTELAMA FIIVAGICLC LYFLFLCFMV FQVFRNISGK QSSLPAMSKV 

       430        440        450        460        470        480 
RRLHYEGLIF RFKFLMLITL ACAAMTVIFF IVSQVSEGHW KWGGVTVQVS SAFFTGIYGM 

       490        500        510        520        530        540 
WNLYVFALMF LYAPSHKNYG EDQSNGDLGV HSGEELQLTT TITHVDGPTE IYKLTRKEAQ 


E 

« Hide

Isoform 2 [UniParc].

Checksum: 483373970A7F1877
Show »

FASTA44151,263

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Embryo, Head, Hippocampus, Ovary, Skin and Uterus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[3]"Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, INTERACTION WITH WNT1; WNT3 AND WNT5A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK019886 mRNA. Translation: BAB31904.1.
AK029053 mRNA. Translation: BAC26266.1. Different initiation.
AK029104 mRNA. Translation: BAC26300.1. Different initiation.
AK044810 mRNA. Translation: BAC32102.1. Different initiation.
AK049842 mRNA. Translation: BAC33950.1. Different initiation.
AK077407 mRNA. Translation: BAC36788.1.
AK159207 mRNA. Translation: BAE34898.1.
BC075615 mRNA. Translation: AAH75615.1.
BC018381 mRNA. Translation: AAH18381.1. Different initiation.
RefSeqNP_080858.3. NM_026582.4.
XP_006502027.1. XM_006501964.1.
UniGeneMm.6766.

3D structure databases

ProteinModelPortalQ6DID7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-49004N.
IntActQ6DID7. 1 interaction.

PTM databases

PhosphoSiteQ6DID7.

Proteomic databases

PaxDbQ6DID7.
PRIDEQ6DID7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000068952; ENSMUSP00000067898; ENSMUSG00000028173. [Q6DID7-1]
GeneID68151.
KEGGmmu:68151.
UCSCuc008rwc.1. mouse. [Q6DID7-1]

Organism-specific databases

CTD79971.
MGIMGI:1915401. Wls.

Phylogenomic databases

eggNOGNOG77798.
GeneTreeENSGT00390000005897.
HOGENOMHOG000039226.
HOVERGENHBG055794.
InParanoidQ6DID7.
OMAKEMSPWF.
OrthoDBEOG7B05CP.
PhylomeDBQ6DID7.
TreeFamTF105975.

Gene expression databases

BgeeQ6DID7.
CleanExMM_GPR177.
GenevestigatorQ6DID7.

Family and domain databases

InterProIPR009551. Wntless.
[Graphical view]
PANTHERPTHR13449. PTHR13449. 1 hit.
ProtoNetSearch...

Other

NextBio326538.
PROQ6DID7.
SOURCESearch...

Entry information

Entry nameWLS_MOUSE
AccessionPrimary (citable) accession number: Q6DID7
Secondary accession number(s): Q8CE42, Q9D2B7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot