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Q6DID7

- WLS_MOUSE

UniProt

Q6DID7 - WLS_MOUSE

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Protein
Protein wntless homolog
Gene
Wls, Gpr177
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development.1 Publication

GO - Molecular functioni

  1. Wnt-protein binding Source: UniProtKB
  2. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. Wnt signaling pathway Source: UniProtKB
  2. anterior/posterior axis specification Source: UniProtKB
  3. mesoderm formation Source: UniProtKB
  4. positive regulation of canonical Wnt signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_213918. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein wntless homolog
Alternative name(s):
Integral membrane protein GPR177
Protein evenness interrupted homolog
Short name:
EVI
Gene namesi
Name:Wls
Synonyms:Gpr177
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1915401. Wls.

Subcellular locationi

Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini43 – 232190Lumenal Reviewed prediction
Add
BLAST
Transmembranei233 – 25321Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini254 – 26815Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei269 – 28921Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini290 – 30314Lumenal Reviewed prediction
Add
BLAST
Transmembranei304 – 32421Helical; Name=3; Reviewed prediction
Add
BLAST
Topological domaini325 – 3317Cytoplasmic Reviewed prediction
Transmembranei332 – 35221Helical; Name=4; Reviewed prediction
Add
BLAST
Topological domaini353 – 38028Lumenal Reviewed prediction
Add
BLAST
Transmembranei381 – 40121Helical; Name=5; Reviewed prediction
Add
BLAST
Topological domaini402 – 43130Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei432 – 45221Helical; Name=6; Reviewed prediction
Add
BLAST
Topological domaini453 – 47119Lumenal Reviewed prediction
Add
BLAST
Transmembranei472 – 49221Helical; Name=7; Reviewed prediction
Add
BLAST
Topological domaini493 – 54149Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB
  2. Golgi membrane Source: UniProtKB-SubCell
  3. cytoplasmic vesicle Source: UniProtKB
  4. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  5. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display embryonic lethality before E10.5. Embryos show defects in the establishment of the body axis and in the primitive streak and mesoderm formation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4242 Reviewed prediction
Add
BLAST
Chaini43 – 541499Protein wntless homolog
PRO_0000271779Add
BLAST

Proteomic databases

MaxQBiQ6DID7.
PaxDbiQ6DID7.
PRIDEiQ6DID7.

PTM databases

PhosphoSiteiQ6DID7.

Expressioni

Tissue specificityi

Expressed in kidney, lung, skin, intestine, brain, spinal cord, skeleton, eyes, excretion glands, tooth and palatal shelves.1 Publication

Developmental stagei

Detected at E6.25 in the proximal epiblast at the junction between the embryonic and extraembryonic tissue. Later expression is more restricted to the primitive streak and mesoderm extending to the distal tip of the embryo. Strong expression is found in both posterior visceral endoderm and epiblast at the prestreak, but switched to the mesoderm at late-streak.1 Publication

Inductioni

Up-regulated by WNT1. Transcriptionally activated by beta-catenin and by LEF/TCF-dependent transcription.1 Publication

Gene expression databases

BgeeiQ6DID7.
CleanExiMM_GPR177.
GenevestigatoriQ6DID7.

Interactioni

Subunit structurei

Interacts with WNT3A By similarity. Interacts with WNT1, WNT3 and WNT5.1 Publication

Protein-protein interaction databases

DIPiDIP-49004N.
IntActiQ6DID7. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ6DID7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 232132Interacts with Wnt proteins
Add
BLAST

Sequence similaritiesi

Belongs to the wntless family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG77798.
GeneTreeiENSGT00390000005897.
HOGENOMiHOG000039226.
HOVERGENiHBG055794.
InParanoidiQ6DID7.
OMAiKEMSPWF.
OrthoDBiEOG7B05CP.
PhylomeDBiQ6DID7.
TreeFamiTF105975.

Family and domain databases

InterProiIPR009551. Wntless.
[Graphical view]
PANTHERiPTHR13449. PTHR13449. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6DID7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGAIIENMS TKKLCIVGGI LLVFQIVAFL VGGLIAPAPT TAVPYTAIKC    50
VDVRKNHHKT RWLAPWGPNK CDKIRDIEEA IPREIEANDI VFSVHIPLPS 100
MEMSPWFQFM LFILQLDIAF KLNNQIRENA EISMDVSLGY RDDMFSEWTE 150
MAHERVPRKL KCTFTSPKTP EHEGRYYNCD VLPFMEIGSV AHKYYLLNIR 200
LPVNEKKKIN VGIGEIKDIR LVGIHQNGGF TKVWFAMKTF LTPSIFIIMV 250
WYWRRITMMS RPPVLLEKVI FALGISMTFI NIPVEWFSIG FDWTWMLLFG 300
DIRQGIFYAM LLSFWIIFCG EHMMDQHERN HIAGYWKQVG PIAVGSFCLF 350
IFDMCERGVQ LTNPFYSIWT TDVGTELAMA FIIVAGICLC LYFLFLCFMV 400
FQVFRNISGK QSSLPAMSKV RRLHYEGLIF RFKFLMLITL ACAAMTVIFF 450
IVSQVSEGHW KWGGVTVQVS SAFFTGIYGM WNLYVFALMF LYAPSHKNYG 500
EDQSNGDLGV HSGEELQLTT TITHVDGPTE IYKLTRKEAQ E 541
Length:541
Mass (Da):62,188
Last modified:August 16, 2004 - v1
Checksum:i14BDD97D6050D976
GO
Isoform 2 (identifier: Q6DID7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:441
Mass (Da):51,263
Checksum:i483373970A7F1877
GO

Sequence cautioni

The sequence AAH18381.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC26266.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC26300.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC32102.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAC33950.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 100100Missing in isoform 2.
VSP_022348Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131I → V in BAC26266. 1 Publication
Sequence conflicti540 – 5401Q → L in BAC26266. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK019886 mRNA. Translation: BAB31904.1.
AK029053 mRNA. Translation: BAC26266.1. Different initiation.
AK029104 mRNA. Translation: BAC26300.1. Different initiation.
AK044810 mRNA. Translation: BAC32102.1. Different initiation.
AK049842 mRNA. Translation: BAC33950.1. Different initiation.
AK077407 mRNA. Translation: BAC36788.1.
AK159207 mRNA. Translation: BAE34898.1.
BC075615 mRNA. Translation: AAH75615.1.
BC018381 mRNA. Translation: AAH18381.1. Different initiation.
CCDSiCCDS38684.1. [Q6DID7-1]
RefSeqiNP_080858.3. NM_026582.4. [Q6DID7-1]
XP_006502027.1. XM_006501964.1. [Q6DID7-1]
UniGeneiMm.6766.

Genome annotation databases

EnsembliENSMUST00000068952; ENSMUSP00000067898; ENSMUSG00000028173. [Q6DID7-1]
GeneIDi68151.
KEGGimmu:68151.
UCSCiuc008rwc.1. mouse. [Q6DID7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK019886 mRNA. Translation: BAB31904.1 .
AK029053 mRNA. Translation: BAC26266.1 . Different initiation.
AK029104 mRNA. Translation: BAC26300.1 . Different initiation.
AK044810 mRNA. Translation: BAC32102.1 . Different initiation.
AK049842 mRNA. Translation: BAC33950.1 . Different initiation.
AK077407 mRNA. Translation: BAC36788.1 .
AK159207 mRNA. Translation: BAE34898.1 .
BC075615 mRNA. Translation: AAH75615.1 .
BC018381 mRNA. Translation: AAH18381.1 . Different initiation.
CCDSi CCDS38684.1. [Q6DID7-1 ]
RefSeqi NP_080858.3. NM_026582.4. [Q6DID7-1 ]
XP_006502027.1. XM_006501964.1. [Q6DID7-1 ]
UniGenei Mm.6766.

3D structure databases

ProteinModelPortali Q6DID7.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-49004N.
IntActi Q6DID7. 1 interaction.

PTM databases

PhosphoSitei Q6DID7.

Proteomic databases

MaxQBi Q6DID7.
PaxDbi Q6DID7.
PRIDEi Q6DID7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068952 ; ENSMUSP00000067898 ; ENSMUSG00000028173 . [Q6DID7-1 ]
GeneIDi 68151.
KEGGi mmu:68151.
UCSCi uc008rwc.1. mouse. [Q6DID7-1 ]

Organism-specific databases

CTDi 79971.
MGIi MGI:1915401. Wls.

Phylogenomic databases

eggNOGi NOG77798.
GeneTreei ENSGT00390000005897.
HOGENOMi HOG000039226.
HOVERGENi HBG055794.
InParanoidi Q6DID7.
OMAi KEMSPWF.
OrthoDBi EOG7B05CP.
PhylomeDBi Q6DID7.
TreeFami TF105975.

Enzyme and pathway databases

Reactomei REACT_213918. WNT ligand biogenesis and trafficking.

Miscellaneous databases

NextBioi 326538.
PROi Q6DID7.
SOURCEi Search...

Gene expression databases

Bgeei Q6DID7.
CleanExi MM_GPR177.
Genevestigatori Q6DID7.

Family and domain databases

InterProi IPR009551. Wntless.
[Graphical view ]
PANTHERi PTHR13449. PTHR13449. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo, Head, Hippocampus, Ovary, Skin and Uterus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
    Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
    Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, INTERACTION WITH WNT1; WNT3 AND WNT5A.

Entry informationi

Entry nameiWLS_MOUSE
AccessioniPrimary (citable) accession number: Q6DID7
Secondary accession number(s): Q8CE42, Q9D2B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: August 16, 2004
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi