Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q6DIC0

- SMCA2_MOUSE

UniProt

Q6DIC0 - SMCA2_MOUSE

Protein

Probable global transcription activator SNF2L2

Gene

Smarca2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (16 Aug 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene By similarity. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi754 – 7618ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. helicase activity Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. RNA polymerase II transcription coactivator activity Source: Ensembl
    5. transcription regulatory region DNA binding Source: Ensembl

    GO - Biological processi

    1. aortic smooth muscle cell differentiation Source: MGI
    2. chromatin organization Source: MGI
    3. negative regulation of cell growth Source: Ensembl
    4. negative regulation of cell proliferation Source: MGI
    5. negative regulation of transcription, DNA-templated Source: Ensembl
    6. nervous system development Source: UniProtKB-KW
    7. nucleosome assembly Source: MGI

    Keywords - Molecular functioni

    Activator, Helicase, Hydrolase

    Keywords - Biological processi

    Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable global transcription activator SNF2L2 (EC:3.6.4.-)
    Alternative name(s):
    ATP-dependent helicase SMARCA2
    BRG1-associated factor 190B
    Short name:
    BAF190B
    Protein brahma homolog
    SNF2-alpha
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
    Gene namesi
    Name:Smarca2
    Synonyms:Baf190b, Brm, Snf2a, Snf2l2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:99603. Smarca2.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. intermediate filament cytoskeleton Source: Ensembl
    2. nBAF complex Source: UniProtKB
    3. npBAF complex Source: UniProtKB
    4. nuclear chromatin Source: Ensembl
    5. nucleus Source: MGI
    6. SWI/SNF complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15771577Probable global transcription activator SNF2L2PRO_0000391618Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei169 – 1691PhosphoserineBy similarity
    Modified residuei334 – 3341Phosphoserine1 Publication
    Modified residuei609 – 6091N6-acetyllysine1 Publication
    Modified residuei1002 – 10021N6-acetyllysineBy similarity
    Modified residuei1004 – 10041N6-acetyllysineBy similarity
    Modified residuei1499 – 14991Phosphoserine1 Publication
    Modified residuei1503 – 15031Phosphoserine1 Publication
    Modified residuei1515 – 15151PhosphoserineBy similarity
    Modified residuei1555 – 15551PhosphoserineBy similarity
    Modified residuei1559 – 15591PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ6DIC0.
    PaxDbiQ6DIC0.
    PRIDEiQ6DIC0.

    PTM databases

    PhosphoSiteiQ6DIC0.

    Expressioni

    Tissue specificityi

    Expressed in the cortex and the hippocampus. Expressed in the cortical plate in the embryo.1 Publication

    Gene expression databases

    ArrayExpressiQ6DIC0.
    BgeeiQ6DIC0.
    GenevestigatoriQ6DIC0.

    Interactioni

    Subunit structurei

    Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Binds TOPBP1 By similarity. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. Interacts with PHF10/BAF45A.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi211982. 34 interactions.
    DIPiDIP-48888N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6DIC0.
    SMRiQ6DIC0. Positions 670-1207, 1383-1491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini167 – 20236QLQPROSITE-ProRule annotationAdd
    BLAST
    Domaini441 – 51373HSAPROSITE-ProRule annotationAdd
    BLAST
    Domaini741 – 906166Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini1059 – 1221163Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini1406 – 147671BromoPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi856 – 8594DEGH box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi4 – 170167Pro-richAdd
    BLAST
    Compositional biasi172 – 340169Gln-richAdd
    BLAST
    Compositional biasi648 – 6558Poly-Glu
    Compositional biasi1302 – 13065Poly-Glu
    Compositional biasi1496 – 151823Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SNF2/RAD54 helicase family.Curated
    Contains 1 bromo domain.PROSITE-ProRule annotation
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 HSA domain.PROSITE-ProRule annotation
    Contains 1 QLQ domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Bromodomain

    Phylogenomic databases

    eggNOGiCOG0553.
    HOGENOMiHOG000172363.
    HOVERGENiHBG056636.
    InParanoidiQ6DIC0.
    KOiK11647.
    PhylomeDBiQ6DIC0.

    Family and domain databases

    Gene3Di1.20.920.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR006576. BRK_domain.
    IPR001487. Bromodomain.
    IPR018359. Bromodomain_CS.
    IPR014978. Gln-Leu-Gln_QLQ.
    IPR013999. HAS_subgr.
    IPR014012. Helicase/SANT-assoc_DNA-bd.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR029295. SnAC.
    IPR000330. SNF2_N.
    [Graphical view]
    PfamiPF07533. BRK. 1 hit.
    PF00439. Bromodomain. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07529. HSA. 1 hit.
    PF14619. SnAC. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00503. BROMODOMAIN.
    SMARTiSM00592. BRK. 1 hit.
    SM00297. BROMO. 1 hit.
    SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00573. HSA. 1 hit.
    SM00951. QLQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF47370. SSF47370. 1 hit.
    SSF52540. SSF52540. 2 hits.
    PROSITEiPS00633. BROMODOMAIN_1. 1 hit.
    PS50014. BROMODOMAIN_2. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51204. HSA. 1 hit.
    PS51666. QLQ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6DIC0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPTDPAAM PHPGPSPGPG PSPGPILGPS PGPGPSPGSV HSMMGPSPGP     50
    PSVSHPLSTM GSADFPQEGM HQLHKPMDGI HDKGIVEDVH CGSMKGTSMR 100
    PPHPGMGPPQ SPMDQHSQGY MSPHPSPLGA PEHVSSPTPP QMPPSQPGAL 150
    IPGDPQAMNQ PNRGPSPFSP VQLHQLRAQI LAYKMLARGQ PLPETLQLAV 200
    QGKRTLPGMQ QQQQQQQQQQ QQQQQQQQQQ QQQQQPQQPQ QQAQAQPQQQ 250
    QQQQQQPALV SYNRPSGPGQ ELLLSGQSAP QKLSAPAPSG RPSPAPQAAV 300
    QPTATAVPGP SVQQPAPGQP SPVLQLQQKQ SRISPIQKPQ GLDPVEILQE 350
    REYRLQARIA HRIQELESLP GSLPPDLRTK ATVELKALRL LNFQRQLRQE 400
    VVACMRRDTT LETALNSKAY KRSKRQTLRE ARMTEKLEKQ QKIEQERKRR 450
    QKHQEYLNSI LQHAKDFKEY HRSVAGKIQK LSKAVATWHA NTEREQKKET 500
    ERIEKERMRR LMAEDEEGYR KLIDQKKDRR LAYLLQQTDE YVANLTNLVW 550
    EHKQAQAAKE KKKRRRRKKK AEENAEGGEP ALGPDGEPID ESSQMSDLPV 600
    KVTHTETGKV LFGPEAPKAS QLDAWLEMNP GYEVAPRSDS EESESDYEEE 650
    DEEEESSRQE TEEKILLDPN SEEVSEKDAK QIIETAKQDV DDEYSMQYSA 700
    RGSQSYYTVA HAISERVEKQ SALLINGTLK HYQLQGLEWM VSLYNNNLNG 750
    ILADEMGLGK TIQTIALITY LMEHKRLNGP YLIIVPLSTL SNWTYEFDKW 800
    APSVVKISYK GTPAMRRSLV PQLRSGKFNV LLTTYEYIIK DKHILAKIRW 850
    KYMIVDEGHR MKNHHCKLTQ VLNTHYVAPR RILLTGTPLQ NKLPELWALL 900
    NFLLPTIFKS CSTFEQWFNA PFAMTGERVD LNEEETILII RRLHKVLRPF 950
    LLRRLKKEVE SQLPEKVEYV IKCDMSALQK ILYRHMQAKG ILLTDGSEKD 1000
    KKGKGGAKTL MNTIMQLRKI CNHPYMFQHI EESFAEHLGY SNGVINGAEL 1050
    YRASGKFELL DRILPKLRAT NHRVLLFCQM TSLMTIMEDY FAFRNFLYLR 1100
    LDGTTKSEDR AALLKKFNEP GSQYFIFLLS TRAGGLGLNL QAADTVVIFD 1150
    SDWNPHQDLQ AQDRAHRIGQ QNEVRVLRLC TVNSVEEKIL AAAKYKLNVD 1200
    QKVIQAGMFD QKSSSHERRA FLQAILEHEE ENEEEDEVPD DETLNQMIAR 1250
    REEEFDLFMR MDMDRRREDA RNPKRKPRLM EEDELPSWII KDDAEVERLT 1300
    CEEEEEKIFG RGSRQRRDVD YSDALTEKQW LRAIEDGNLE EMEEEVRLKK 1350
    RKRRRNVDKD PVKEDVEKAK KRRGRPPAEK LSPNPPKLTK QMNAIIDTVI 1400
    NYKDSSGRQL SEVFIQLPSR KDLPEYYELI RKPVDFKKIK ERIRNHKYRS 1450
    LGDLEKDVML LCHNAQTFNL EGSQIYEDSI VLQSVFKSAR QKIAKEEESE 1500
    EESNEEEEED DEEESESEAK SVKVKIKLNK KEEKGRDTGK GKKRPNRGKA 1550
    KPVVSDFDSD EEQEENEQSE ASGTDNE 1577
    Length:1,577
    Mass (Da):180,253
    Last modified:August 16, 2004 - v1
    Checksum:i5D79E8FFC66085BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC075641 mRNA. Translation: AAH75641.1.
    RefSeqiNP_035546.2. NM_011416.2.
    UniGeneiMm.491146.

    Genome annotation databases

    GeneIDi67155.
    KEGGimmu:67155.
    UCSCiuc012bjw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC075641 mRNA. Translation: AAH75641.1 .
    RefSeqi NP_035546.2. NM_011416.2.
    UniGenei Mm.491146.

    3D structure databases

    ProteinModelPortali Q6DIC0.
    SMRi Q6DIC0. Positions 670-1207, 1383-1491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211982. 34 interactions.
    DIPi DIP-48888N.

    PTM databases

    PhosphoSitei Q6DIC0.

    Proteomic databases

    MaxQBi Q6DIC0.
    PaxDbi Q6DIC0.
    PRIDEi Q6DIC0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 67155.
    KEGGi mmu:67155.
    UCSCi uc012bjw.1. mouse.

    Organism-specific databases

    CTDi 6595.
    MGIi MGI:99603. Smarca2.

    Phylogenomic databases

    eggNOGi COG0553.
    HOGENOMi HOG000172363.
    HOVERGENi HBG056636.
    InParanoidi Q6DIC0.
    KOi K11647.
    PhylomeDBi Q6DIC0.

    Miscellaneous databases

    ChiTaRSi SMARCA2. mouse.
    NextBioi 323746.
    PROi Q6DIC0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q6DIC0.
    Bgeei Q6DIC0.
    Genevestigatori Q6DIC0.

    Family and domain databases

    Gene3Di 1.20.920.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR006576. BRK_domain.
    IPR001487. Bromodomain.
    IPR018359. Bromodomain_CS.
    IPR014978. Gln-Leu-Gln_QLQ.
    IPR013999. HAS_subgr.
    IPR014012. Helicase/SANT-assoc_DNA-bd.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR029295. SnAC.
    IPR000330. SNF2_N.
    [Graphical view ]
    Pfami PF07533. BRK. 1 hit.
    PF00439. Bromodomain. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07529. HSA. 1 hit.
    PF14619. SnAC. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00503. BROMODOMAIN.
    SMARTi SM00592. BRK. 1 hit.
    SM00297. BROMO. 1 hit.
    SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    SM00573. HSA. 1 hit.
    SM00951. QLQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47370. SSF47370. 1 hit.
    SSF52540. SSF52540. 2 hits.
    PROSITEi PS00633. BROMODOMAIN_1. 1 hit.
    PS50014. BROMODOMAIN_2. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51204. HSA. 1 hit.
    PS51666. QLQ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "An essential switch in subunit composition of a chromatin remodeling complex during neural development."
      Lessard J., Wu J.I., Ranish J.A., Wan M., Winslow M.M., Staahl B.T., Wu H., Aebersold R., Graef I.A., Crabtree G.R.
      Neuron 55:201-215(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NBAF AND NPBAF COMPLEXES, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE NBAF AND NPBAF COMPLEXES, INTERACTION WITH PHF10.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-334; SER-1499 AND SER-1503, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "Heterozygous missense mutations in SMARCA2 cause Nicolaides-Baraitser syndrome."
      Van Houdt J.K., Nowakowska B.A., Sousa S.B., van Schaik B.D., Seuntjens E., Avonce N., Sifrim A., Abdul-Rahman O.A., van den Boogaard M.J., Bottani A., Castori M., Cormier-Daire V., Deardorff M.A., Filges I., Fryer A., Fryns J.P., Gana S., Garavelli L.
      , Gillessen-Kaesbach G., Hall B.D., Horn D., Huylebroeck D., Klapecki J., Krajewska-Walasek M., Kuechler A., Lines M.A., Maas S., Macdermot K.D., McKee S., Magee A., de Man S.A., Moreau Y., Morice-Picard F., Obersztyn E., Pilch J., Rosser E., Shannon N., Stolte-Dijkstra I., Van Dijck P., Vilain C., Vogels A., Wakeling E., Wieczorek D., Wilson L., Zuffardi O., van Kampen A.H., Devriendt K., Hennekam R., Vermeesch J.R.
      Nat. Genet. 44:445-449(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-609, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiSMCA2_MOUSE
    AccessioniPrimary (citable) accession number: Q6DIC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 9, 2010
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3