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Q6DIB5

- MEG10_MOUSE

UniProt

Q6DIB5 - MEG10_MOUSE

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Protein

Multiple epidermal growth factor-like domains protein 10

Gene
Megf10
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Membrane receptor involved in phagocytosis by macrophages of apoptotic cells. Cooperates with ABCA1 within the process of engulfment. Promotes the formation of large intracellular vacuoles and may be responsible for the uptake of amyloid-beta peptides. May also function in the mosaic spacing of specific neuron subtypes in the retina through homotypic retinal neuron repulsion. Mosaics provide a mechanism to distribute each cell type evenly across the retina, ensuring that all parts of the visual field have access to a full set of processing elements. May play role in cell adhesion and motility. Is also an essential factor in the regulation of myogenesis. Controls the balance between skeletal muscle satellite cells proliferation and differentiation problably through regulation of the notch signaling pathway.3 Publications

GO - Biological processi

  1. homotypic cell-cell adhesion Source: UniProtKB
  2. muscle cell development Source: UniProtKB
  3. recognition of apoptotic cell Source: MGI
  4. regulation of muscle cell differentiation Source: UniProtKB
  5. regulation of skeletal muscle tissue development Source: Ensembl
  6. satellite cell activation Source: UniProtKB
  7. satellite cell differentiation Source: UniProtKB
  8. satellite cell proliferation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Myogenesis, Phagocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Multiple epidermal growth factor-like domains protein 10
Short name:
Multiple EGF-like domains protein 10
Gene namesi
Name:Megf10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:2685177. Megf10.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein. Basolateral cell membrane; Single-pass type I membrane protein By similarity. Cell projectionphagocytic cup By similarity
Note: Forms an irregular, mosaic-like adhesion pattern in region of the cell surface that becomes firmely fixed to the substrate. Expressed at the cell surface in clusters around cell corpses during engulfment. During the engulfment of apoptotic thymocytes, recruited at the bottom of the forming phagocytic cup. Colocalizes with ABCA1 in absence of any phagocytic challenge. Does not localize within lamellipodia. Does not localize with MEGF11 By similarity. Enriched at the sites of contact with apoptotic thymocyte cells.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 857832Extracellular Reviewed predictionAdd
BLAST
Transmembranei858 – 87821Helical; Reviewed predictionAdd
BLAST
Topological domaini879 – 1147269Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. cell projection Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
  4. phagocytic cup Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed predictionAdd
BLAST
Chaini26 – 11471122Multiple epidermal growth factor-like domains protein 10PRO_0000309733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi34 ↔ 95 Reviewed prediction
Disulfide bondi60 ↔ 69 Reviewed prediction
Disulfide bondi94 ↔ 105 Reviewed prediction
Disulfide bondi109 ↔ 124 By similarity
Disulfide bondi126 ↔ 135 By similarity
Glycosylationi134 – 1341N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi148 ↔ 160 By similarity
Disulfide bondi154 ↔ 167 By similarity
Disulfide bondi169 ↔ 178 By similarity
Disulfide bondi191 ↔ 203 By similarity
Disulfide bondi197 ↔ 210 By similarity
Disulfide bondi212 ↔ 221 By similarity
Disulfide bondi234 ↔ 246 By similarity
Disulfide bondi240 ↔ 253 By similarity
Disulfide bondi255 ↔ 264 By similarity
Disulfide bondi281 ↔ 289 By similarity
Disulfide bondi283 ↔ 296 By similarity
Disulfide bondi298 ↔ 307 By similarity
Disulfide bondi320 ↔ 332 By similarity
Disulfide bondi326 ↔ 339 By similarity
Disulfide bondi341 ↔ 350 By similarity
Disulfide bondi409 ↔ 421 By similarity
Disulfide bondi415 ↔ 428 By similarity
Disulfide bondi430 ↔ 439 By similarity
Disulfide bondi456 ↔ 464 By similarity
Disulfide bondi458 ↔ 471 By similarity
Disulfide bondi473 ↔ 482 By similarity
Disulfide bondi495 ↔ 507 By similarity
Glycosylationi496 – 4961N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi501 ↔ 514 By similarity
Disulfide bondi516 ↔ 525 By similarity
Disulfide bondi542 ↔ 550 By similarity
Disulfide bondi544 ↔ 557 By similarity
Disulfide bondi559 ↔ 568 By similarity
Disulfide bondi581 ↔ 593 By similarity
Disulfide bondi587 ↔ 600 By similarity
Disulfide bondi602 ↔ 611 By similarity
Disulfide bondi669 ↔ 681 By similarity
Disulfide bondi675 ↔ 688 By similarity
Disulfide bondi690 ↔ 699 By similarity
Disulfide bondi716 ↔ 724 By similarity
Disulfide bondi718 ↔ 731 By similarity
Disulfide bondi733 ↔ 742 By similarity
Disulfide bondi755 ↔ 767 By similarity
Disulfide bondi761 ↔ 774 By similarity
Disulfide bondi776 ↔ 785 By similarity
Disulfide bondi802 ↔ 810 By similarity
Disulfide bondi804 ↔ 817 By similarity
Disulfide bondi819 ↔ 828 By similarity

Post-translational modificationi

Phosphorylated on tyrosine residues By similarity.
Ubiquitinated; mono- and polyubiquitinated forms are detected By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ6DIB5.
PRIDEiQ6DIB5.

PTM databases

PhosphoSiteiQ6DIB5.

Expressioni

Tissue specificityi

Expressed in cerebellum (at protein level). Expressed in kidney, stellate cells of the cerebellum and macrophage cell lines.3 Publications

Developmental stagei

Expressed in embryo at 15 dpc (at protein level). Expressed in embryo at 8, 10, 11, 13, 14 and 15 dpc.1 Publication

Gene expression databases

BgeeiQ6DIB5.
CleanExiMM_MEGF10.
GenevestigatoriQ6DIB5.

Interactioni

Subunit structurei

Homopolymer Inferred. Interacts with GULP1 and ABCA1. Interacts with AP2M1. Does not interact with MEGF11 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ6DIB5.
SMRiQ6DIB5. Positions 93-833.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 10778EMIAdd
BLAST
Domaini101 – 13636EGF-like 1Add
BLAST
Domaini144 – 17936EGF-like 2Add
BLAST
Domaini187 – 22236EGF-like 3Add
BLAST
Domaini230 – 26536EGF-like 4Add
BLAST
Domaini278 – 30831EGF-like 5Add
BLAST
Domaini316 – 35136EGF-like 6Add
BLAST
Domaini405 – 44036EGF-like 7Add
BLAST
Domaini453 – 48331EGF-like 8Add
BLAST
Domaini491 – 52636EGF-like 9Add
BLAST
Domaini539 – 56931EGF-like 10Add
BLAST
Domaini577 – 61236EGF-like 11Add
BLAST
Domaini665 – 70036EGF-like 12Add
BLAST
Domaini713 – 74331EGF-like 13Add
BLAST
Domaini751 – 78636EGF-like 14Add
BLAST
Domaini799 – 82931EGF-like 15Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 857857Necessary for interaction with AP2M1, self-assembly and formation of the irregular, mosaic-like adhesion pattern By similarityAdd
BLAST
Regioni945 – 1147203Necessary for formation of large intracellular vacuoles By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1119 – 114628Ser-richAdd
BLAST

Domaini

The EMI and EGF-like domains work in concert to promote self-assembly.

Sequence similaritiesi

Belongs to the MEGF family.
Contains 15 EGF-like domains.
Contains 1 EMI domain.

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00730000110380.
HOGENOMiHOG000294130.
HOVERGENiHBG108333.
InParanoidiQ6DIB5.
OMAiFGCNLTC.
OrthoDBiEOG72C50D.
PhylomeDBiQ6DIB5.
TreeFamiTF332598.

Family and domain databases

InterProiIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR002049. EGF_laminin.
IPR011489. EMI_domain.
[Graphical view]
PfamiPF12661. hEGF. 4 hits.
PF00053. Laminin_EGF. 5 hits.
[Graphical view]
SMARTiSM00181. EGF. 12 hits.
SM00180. EGF_Lam. 5 hits.
[Graphical view]
PROSITEiPS00022. EGF_1. 17 hits.
PS01186. EGF_2. 17 hits.
PS50026. EGF_3. 15 hits.
PS51041. EMI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6DIB5-1 [UniParc]FASTAAdd to Basket

« Hide

MAISSSSCLG LICSLLCHWV GTASSLNLED PNVCSHWESY SVTVQESYPH     50
PFDQIYYTSC TDILNWFKCT RHRISYRTAY RHGEKTMYRR KSQCCPGFYE 100
SRDMCVPHCA DKCVHGRCIA PNTCQCEPGW GGTNCSSACD GDHWGPHCSS 150
RCQCKNRALC NPITGACHCA AGYRGWRCED RCEQGTYGND CHQRCQCQNG 200
ATCDHITGEC RCSPGYTGAF CEDLCPPGKH GPHCEQRCPC QNGGVCHHVT 250
GECSCPSGWM GTVCGQPCPE GRFGKNCSQE CQCHNGGTCD AATGQCHCSP 300
GYTGERCQDE CPVGSYGVRC AEACRCVNGG KCYHVSGTCL CEAGFSGELC 350
EARLCPEGLY GIKCDKRCPC HLDNTHSCHP MSGECGCKPG WSGLYCNETC 400
SPGFYGEACQ QICSCQNGAD CDSVTGRCAC APGFKGTDCS TPCPLGRYGI 450
NCSSRCGCKN DAVCSPVDGS CICKAGWHGV DCSIRCPSGT WGFGCNLTCQ 500
CLNGGACNTL DGTCTCAPGW RGAKCEFPCQ DGTYGLNCAE RCDCSHADGC 550
HPTTGHCRCL PGWSGVHCDS VCAEGRWGPN CSLPCYCKNG ASCSPDDGIC 600
ECAPGFRGTT CQRICSPGFY GHRCSQTCPQ CVHSSGPCHH ITGLCDCLPG 650
FTGALCNEVC PSGRFGKNCA GVCTCTNNGT CNPIDRSCQC YPGWIGSDCS 700
QPCPPAHWGP NCIHTCNCHN GAFCSAYDGE CKCTPGWTGL YCTQRCPLGF 750
YGKDCALICQ CQNGADCDHI SGQCTCRTGF MGRHCEQKCP AGTYGYGCRQ 800
ICDCLNNSTC DHITGTCYCS PGWKGARCDQ AGVIIVGNLN SLSRTSTALP 850
ADSYQIGAIA GIVVLVLVVL FLLALFIIYR HKQKRKESSM PAVTYTPAMR 900
VINADYTIAE TLPHSNGGNA NSHYFTNPSY HTLSQCATSP HVNNRDRMTI 950
AKSKNNQLFV NLKNVNPGKR GTLVDCTGTL PADWKQGGYL NELGAFGLDR 1000
SYMGKSLKDL GKNSEYNSST CSLSSSENPY ATIKDPPALL PKSSECGYVE 1050
MKSPARRDSP YAEINNSTPA NRNVYEVEPT VSVVQGVFSN SGHVTQDPYD 1100
LPKNSHIPCH YDLLPVRDSS SSPKREDGGG SNSTSSNSTS SSSSSSE 1147
Length:1,147
Mass (Da):122,972
Last modified:August 16, 2004 - v1
Checksum:iFBC50896096181CC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti323 – 3231A → T in BAE38699. 1 Publication
Sequence conflicti1092 – 10921G → S in BAE38699. 1 Publication
Sequence conflicti1137 – 11371N → S in BAE38699. 1 Publication
Sequence conflicti1140 – 11401S → T in BAE38699. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK147238 mRNA. Translation: BAE27788.1.
AK148084 mRNA. Translation: BAE28336.1.
AK166316 mRNA. Translation: BAE38699.1.
BC075647 mRNA. Translation: AAH75647.1.
CCDSiCCDS29264.1.
RefSeqiNP_001001979.1. NM_001001979.2.
UniGeneiMm.297863.

Genome annotation databases

EnsembliENSMUST00000075770; ENSMUSP00000075174; ENSMUSG00000024593.
GeneIDi70417.
KEGGimmu:70417.
UCSCiuc008eyz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK147238 mRNA. Translation: BAE27788.1 .
AK148084 mRNA. Translation: BAE28336.1 .
AK166316 mRNA. Translation: BAE38699.1 .
BC075647 mRNA. Translation: AAH75647.1 .
CCDSi CCDS29264.1.
RefSeqi NP_001001979.1. NM_001001979.2.
UniGenei Mm.297863.

3D structure databases

ProteinModelPortali Q6DIB5.
SMRi Q6DIB5. Positions 93-833.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q6DIB5.

Proteomic databases

PaxDbi Q6DIB5.
PRIDEi Q6DIB5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000075770 ; ENSMUSP00000075174 ; ENSMUSG00000024593 .
GeneIDi 70417.
KEGGi mmu:70417.
UCSCi uc008eyz.2. mouse.

Organism-specific databases

CTDi 84466.
MGIi MGI:2685177. Megf10.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00730000110380.
HOGENOMi HOG000294130.
HOVERGENi HBG108333.
InParanoidi Q6DIB5.
OMAi FGCNLTC.
OrthoDBi EOG72C50D.
PhylomeDBi Q6DIB5.
TreeFami TF332598.

Miscellaneous databases

ChiTaRSi MEGF10. mouse.
NextBioi 331567.
PROi Q6DIB5.
SOURCEi Search...

Gene expression databases

Bgeei Q6DIB5.
CleanExi MM_MEGF10.
Genevestigatori Q6DIB5.

Family and domain databases

InterProi IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR002049. EGF_laminin.
IPR011489. EMI_domain.
[Graphical view ]
Pfami PF12661. hEGF. 4 hits.
PF00053. Laminin_EGF. 5 hits.
[Graphical view ]
SMARTi SM00181. EGF. 12 hits.
SM00180. EGF_Lam. 5 hits.
[Graphical view ]
PROSITEi PS00022. EGF_1. 17 hits.
PS01186. EGF_2. 17 hits.
PS50026. EGF_3. 15 hits.
PS51041. EMI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Cooperation between engulfment receptors: the case of ABCA1 and MEGF10."
    Hamon Y., Trompier D., Ma Z., Venegas V., Pophillat M., Mignotte V., Zhou Z., Chimini G.
    PLoS ONE 1:E120-E120(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  4. "MEGF10 is a mammalian ortholog of CED-1 that interacts with clathrin assembly protein complex 2 medium chain and induces large vacuole formation."
    Suzuki E., Nakayama M.
    Exp. Cell Res. 313:3729-3742(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  5. "Megf10 regulates the progression of the satellite cell myogenic program."
    Holterman C.E., Le Grand F., Kuang S., Seale P., Rudnicki M.A.
    J. Cell Biol. 179:911-922(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MYOGENESIS, TISSUE SPECIFICITY.
  6. "MEGF10 functions as a receptor for the uptake of amyloid-beta."
    Singh T.D., Park S.Y., Bae J.S., Yun Y., Bae Y.C., Park R.W., Kim I.S.
    FEBS Lett. 584:3936-3942(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ENDOCYTOSIS.
  7. "MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons."
    Kay J.N., Chu M.W., Sanes J.R.
    Nature 483:465-469(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEURONAL MOSAIC SPACING.

Entry informationi

Entry nameiMEG10_MOUSE
AccessioniPrimary (citable) accession number: Q6DIB5
Secondary accession number(s): Q3TLU3, Q3UG73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi