Reviewed, UniProtKB/Swiss-Prot Q6DHU8 (TOPK_DANRE)

Names and origin

Protein names

Recommended name:
    Lymphokine-activated killer T-cell-originated protein kinase homolog
    EC=2.7.12.2
Alternative name(s):
    PDZ-binding kinase

Gene names

Name:	pbk
Synonyms:	topk
ORF Names:	zgc:92050

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by phosphorylation By similarity.
Post-translational modification	Phosphorylated; in a cell-cycle dependent manner at mitosis By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAI20933.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Lymphokine-activated killer T-cell-originated protein kinase homolog		PRO_0000086765
Regions
	Domain	36 – 328	293	Protein kinase
	Nucleotide binding	42 – 50	9	ATP By similarity
Sites
	Active site	170	1	Proton acceptor By similarity
	Binding site	67	1	ATP By similarity
Amino acid modifications
	Modified residue	203	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DHU8-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 696EDB59DCADC2CE	FASTA	339	37,664
	10 20 30 40 50 60 MEASKESPSA FKTPCKPAKV KSPVNACGTP VTIPASPFMK KLGCGTGVNV YLMNRMGKQT 70 80 90 100 110 120 HSPWAIKKIN SKCAQGQVSV YQKRLCEEAK ILKDLKHPNI VGFRAFTTAK DGSKCLAMEF 130 140 150 160 170 180 GGEQSLNDLI EKRREEGLQA FPVDTIEKVA LHVARGLLYL HNEKKLLHGD MKSCNVVIKG 190 200 210 220 230 240 DFESIKICDV GVSLPLDENM QVSDPKAHYI GTEPWKPKEA LEDGVITDKA DIFAYGLTLW 250 260 270 280 290 300 EMMTLSVPHL EMLDTEGDED DDDESFEEDF DEDAYYERLG SRPALDAVTL GGSYQRMVEL 310 320 330 FCLCTEEDPQ KRPSAAHIVQ VLESNSQLSK QNTEVIVID

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL845306 Genomic DNA. Translation: CAI20932.1. AL845306 Genomic DNA. Translation: CAI20933.1. Sequence problems. BC075869 mRNA. Translation: AAH75869.1.
IPI	IPI00483477.
RefSeq	NP_001002387.1.
UniGene	Dr.77309
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6DHU8.
Proteomic databases
PRIDE	Q6DHU8.
Genome annotation databases
Ensembl	ENSDART00000021788; ENSDARP00000013651; ENSDARG00000007221; Danio rerio. [Genome view]
GeneID	360218.
KEGG	dre:360218.
Organism-specific databases
CTD	360218.
ZFIN	ZDB-GENE-030523-2. pbk.
Phylogenomic databases
HOGENOM	Q6DHU8.
HOVERGEN	Q6DHU8.
OMA	ALEMDVQ.
Enzyme and pathway databases
BRENDA	2.7.12.2. 96826.
Gene expression databases
Bgee	Q6DHU8.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOPK_DANRE

Accession

Primary (citable) accession number: Q6DHU8
Secondary accession number(s): Q5SPR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project