ID   Q6DGU9_DANRE            Unreviewed;       229 AA.
AC   Q6DGU9; A0A8M1N0N7;
DT   16-AUG-2004, integrated into UniProtKB/TrEMBL.
DT   16-AUG-2004, sequence version 1.
DT   27-MAR-2024, entry version 133.
DE   RecName: Full=Glutathione S-transferase kappa {ECO:0000256|PIRNR:PIRNR006386};
DE            EC=2.5.1.18 {ECO:0000256|PIRNR:PIRNR006386};
GN   Name=gstk1 {ECO:0000313|Ensembl:ENSDARP00000035103,
GN   ECO:0000313|RefSeq:NP_001002560.1, ECO:0000313|RefSeq:XP_005158401.1,
GN   ECO:0000313|ZFIN:ZDB-GENE-040718-298};
GN   ORFNames=zgc:92763 {ECO:0000313|EMBL:AAH76240.1,
GN   ECO:0000313|RefSeq:NP_001002560.1, ECO:0000313|RefSeq:XP_005158401.1};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955 {ECO:0000313|EMBL:AAH76240.1};
RN   [1] {ECO:0000313|EMBL:AAH76240.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain {ECO:0000313|EMBL:AAH76240.1};
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSDARP00000035103}
RP   IDENTIFICATION.
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000035103};
RG   Ensembl;
RL   Submitted (FEB-2012) to UniProtKB.
RN   [3] {ECO:0000313|RefSeq:NP_001002560.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=23770340;
RA   Timme-Laragy A.R., Goldstone J.V., Imhoff B.R., Stegeman J.J., Hahn M.E.,
RA   Hansen J.M.;
RT   "Glutathione redox dynamics and expression of glutathione-related genes in
RT   the developing embryo.";
RL   Free Radic. Biol. Med. 65:89-101(2013).
RN   [4] {ECO:0000313|Ensembl:ENSDARP00000035103, ECO:0000313|Proteomes:UP000000437}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000035103};
RX   PubMed=23594743; DOI=10.1038/nature12111;
RG   Genome Reference Consortium Zebrafish;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA   Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA   Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA   Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA   Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA   Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA   Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA   Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA   Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA   Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA   Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA   Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA   Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA   Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA   Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA   Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA   Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA   Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA   Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA   Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA   Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA   Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA   Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [5] {ECO:0000313|RefSeq:NP_001002560.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=23684558;
RA   Knecht A.L., Goodale B.C., Truong L., Simonich M.T., Swanson A.J.,
RA   Matzke M.M., Anderson K.A., Waters K.M., Tanguay R.L.;
RT   "Comparative developmental toxicity of environmentally relevant oxygenated
RT   PAHs.";
RL   Toxicol. Appl. Pharmacol. 271:266-275(2013).
RN   [6] {ECO:0000313|RefSeq:NP_001002560.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=25461745;
RA   Glisic B., Mihaljevic I., Popovic M., Zaja R., Loncar J., Fent K.,
RA   Kovacevic R., Smital T.;
RT   "Characterization of glutathione-S-transferases in zebrafish (Danio
RT   rerio).";
RL   Aquat. Toxicol. 158:50-62(2015).
RN   [7] {ECO:0000313|RefSeq:NP_001002560.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=26469318;
RA   Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M.,
RA   Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D.,
RA   Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E.,
RA   Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M.,
RA   Reith W., Hertzano R.;
RT   "RFX transcription factors are essential for hearing in mice.";
RL   Nat. Commun. 6:8549-8549(2015).
RN   [8] {ECO:0000313|RefSeq:NP_001002560.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=27086301; DOI=10.1007/s00128-016-1798-3;
RA   Garcia-Reyero N., Escalon L., Prats E., Faria M., Soares A.M., Raldua D.;
RT   "Targeted Gene Expression in Zebrafish Exposed to Chlorpyrifos-Oxon
RT   Confirms Phenotype-Specific Mechanisms Leading to Adverse Outcomes.";
RL   Bull. Environ. Contam. Toxicol. 96:707-713(2016).
RN   [9] {ECO:0000313|RefSeq:NP_001002560.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=27378925;
RA   Sasagawa S., Nishimura Y., Okabe S., Murakami S., Ashikawa Y., Yuge M.,
RA   Kawaguchi K., Kawase R., Okamoto R., Ito M., Tanaka T.;
RT   "Downregulation of GSTK1 Is a Common Mechanism Underlying Hypertrophic
RT   Cardiomyopathy.";
RL   Front. Pharmacol. 7:162-162(2016).
RN   [10] {ECO:0000313|RefSeq:NP_001002560.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=27494266;
RA   Han B., Li W., Chen Z., Xu Q., Luo J., Shi Y., Li X., Yan X., Zhang J.;
RT   "Variation of DNA Methylome of Zebrafish Cells under Cold Pressure.";
RL   PLoS ONE 11:e0160358-e0160358(2016).
RN   [11] {ECO:0000313|RefSeq:NP_001002560.1}
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=28252024;
RA   Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A.,
RA   Choudhary J.S., Emes R.D., Grant S.G.;
RT   "Evolution of complexity in the zebrafish synapse proteome.";
RL   Nat. Commun. 8:14613-14613(2017).
RN   [12] {ECO:0000313|RefSeq:NP_001002560.1, ECO:0000313|RefSeq:XP_005158401.1}
RP   IDENTIFICATION.
RC   STRAIN=Tuebingen {ECO:0000313|RefSeq:XP_005158401.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glutathione + RX = a halide anion + an S-substituted
CC         glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925,
CC         ChEBI:CHEBI:90779; EC=2.5.1.18;
CC         Evidence={ECO:0000256|ARBA:ARBA00000710,
CC         ECO:0000256|PIRNR:PIRNR006386};
CC   -!- SIMILARITY: Belongs to the GST superfamily. Kappa family.
CC       {ECO:0000256|ARBA:ARBA00006494, ECO:0000256|PIRNR:PIRNR006386}.
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DR   EMBL; CR854927; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC076240; AAH76240.1; -; mRNA.
DR   RefSeq; NP_001002560.1; NM_001002560.1.
DR   RefSeq; XP_005158401.1; XM_005158344.4.
DR   STRING; 7955.ENSDARP00000035103; -.
DR   PaxDb; 7955-ENSDARP00000035103; -.
DR   Ensembl; ENSDART00000030282.9; ENSDARP00000035103.7; ENSDARG00000056510.8.
DR   GeneID; 436833; -.
DR   KEGG; dre:436833; -.
DR   AGR; ZFIN:ZDB-GENE-040718-298; -.
DR   CTD; 373156; -.
DR   ZFIN; ZDB-GENE-040718-298; gstk1.
DR   eggNOG; ENOG502RH77; Eukaryota.
DR   HOGENOM; CLU_069253_1_1_1; -.
DR   OMA; ECTNSKG; -.
DR   OrthoDB; 4159077at2759; -.
DR   TreeFam; TF105323; -.
DR   Reactome; R-DRE-156590; Glutathione conjugation.
DR   Reactome; R-DRE-9033241; Peroxisomal protein import.
DR   Proteomes; UP000000437; Chromosome 16.
DR   Bgee; ENSDARG00000056510; Expressed in muscle tissue and 25 other cell types or tissues.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005777; C:peroxisome; IBA:GO_Central.
DR   GO; GO:0004602; F:glutathione peroxidase activity; IBA:GO_Central.
DR   GO; GO:0004364; F:glutathione transferase activity; IBA:GO_Central.
DR   GO; GO:0006749; P:glutathione metabolic process; IBA:GO_Central.
DR   CDD; cd03021; DsbA_GSTK; 1.
DR   Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR   InterPro; IPR001853; DSBA-like_thioredoxin_dom.
DR   InterPro; IPR044088; GSTK.
DR   InterPro; IPR014440; HCCAis_GSTk.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR42943; GLUTATHIONE S-TRANSFERASE KAPPA; 1.
DR   PANTHER; PTHR42943:SF2; GLUTATHIONE S-TRANSFERASE KAPPA 1; 1.
DR   Pfam; PF01323; DSBA; 1.
DR   PIRSF; PIRSF006386; HCCAis_GSTk; 1.
DR   SUPFAM; SSF52833; Thioredoxin-like; 1.
PE   1: Evidence at protein level;
KW   Proteomics identification {ECO:0007829|PeptideAtlas:Q6DGU9};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR006386}.
FT   DOMAIN          7..213
FT                   /note="DSBA-like thioredoxin"
FT                   /evidence="ECO:0000259|Pfam:PF01323"
FT   ACT_SITE        16
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR006386-1"
SQ   SEQUENCE   229 AA;  25907 MW;  6BA2C27DDA0C1B05 CRC64;
     MSGSRKVVEL FYDVVSPYSW LAFEVLCRYR NVWNIDLKLK PAFLGAVMHD SGNRPPGMIP
     NKFLYMTSDL KHVSEYFGVP VRQPSNVFET MFEKGSLKAM RFVTAVAEKE KEGDVKLERV
     SRELWNRIWS NDQDITLPAS FAEAGLKAGL TASEVDELLT LATSQPIKDK LKSVTSEAIK
     YKCFGFPTIV CHVDGKPEIF FGSDRFEVMA HIIGEKWMGP NPVKPMAKM
//