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Protein

Nucleolar protein 58

Gene

Nop58

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for 60S ribosomal subunit biogenesis (By similarity). Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles. Required for the biogenesis of box C/D snoRNAs such as U3, U8 and U14 snoRNAs (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

ReactomeiR-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-6791226. Major pathway of rRNA processing in the nucleolus.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 58
Alternative name(s):
MSSP
Nucleolar protein 5
SIK-similar protein
Gene namesi
Name:Nop58
Synonyms:Nol5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1933184. Nop58.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 536536Nucleolar protein 58PRO_0000287350Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei109 – 1091PhosphoserineBy similarity
Modified residuei351 – 3511PhosphoserineBy similarity
Cross-linki415 – 415Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki467 – 467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki467 – 467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki504 – 504Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki504 – 504Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei509 – 5091PhosphoserineCombined sources
Modified residuei521 – 5211PhosphoserineCombined sources

Post-translational modificationi

Sumoylation is essential for high-affinity binding to snoRNAs.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ6DFW4.
MaxQBiQ6DFW4.
PaxDbiQ6DFW4.
PRIDEiQ6DFW4.

PTM databases

iPTMnetiQ6DFW4.
PhosphoSiteiQ6DFW4.

Expressioni

Gene expression databases

BgeeiQ6DFW4.
CleanExiMM_NOL5.
ExpressionAtlasiQ6DFW4. baseline and differential.
GenevisibleiQ6DFW4. MM.

Interactioni

Subunit structurei

Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles; the core proteins SNU13, NOP56, NOP58 and FBL assemble stepwise onto the snoRNA. Interacts with NOLC1/Nopp140. Interacts with NUFIP1, RUVBL1 AND RUVBL2; RUVBL1:RUVBL2 seem to bridge the association of NOP58 with NUFIP1. Interacts with PIH1D1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207760. 1 interaction.
IntActiQ6DFW4. 4 interactions.
MINTiMINT-1854137.
STRINGi10090.ENSMUSP00000027174.

Structurei

3D structure databases

ProteinModelPortaliQ6DFW4.
SMRiQ6DFW4. Positions 142-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini282 – 400119NopPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi441 – 53292Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the NOP5/NOP56 family.Curated
Contains 1 Nop domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2572. Eukaryota.
COG1498. LUCA.
GeneTreeiENSGT00550000074998.
HOGENOMiHOG000196310.
InParanoidiQ6DFW4.
KOiK14565.
OMAiIRNQMEG.
OrthoDBiEOG7T7GT0.
PhylomeDBiQ6DFW4.
TreeFamiTF105688.

Family and domain databases

InterProiIPR012974. NOP5_N.
IPR002687. Nop_dom.
IPR012976. NOSIC.
[Graphical view]
PfamiPF01798. Nop. 1 hit.
PF08156. NOP5NT. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DFW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVLFETSVG YAIFKVLNEK KLQEVDSLWK EFETPEKANK IVKLKHFEKF
60 70 80 90 100
QDTAEALAAF TALMEGKINK QLKKVLKKIV KEAHEPLAVA DAKLGGVIKE
110 120 130 140 150
KLNLSCIHSP VVNELMRGIR SQMDGLIPGV EPREMAAMCL GLAHSLSRYR
160 170 180 190 200
LKFSADKVDT MIVQAISLLD DLDKELNNYI MRCREWYGWH FPELGKIISD
210 220 230 240 250
NLTYCKCLQK VGDRKNYASA SLSEFLSEEV EAEVKAAAEI SMGTEVSEED
260 270 280 290 300
ICNILHLCTQ VIEISEYRTQ LYEYLQNRMM AIAPNVTVMV GELVGARLIA
310 320 330 340 350
HAGSLLNLAK HAASTVQILG AEKALFRALK SRRDTPKYGL IYHASLVGQS
360 370 380 390 400
SPKHKGKISR MLAAKTVLAI RYDAFGEDSS SAMGIENRAK LEARLRILED
410 420 430 440 450
RGIRKISGTG KALAKAEKYE HKSEVKTYDP SGDSTLPTCS KKRKIEEVDK
460 470 480 490 500
EDEITEKKAK KAKIKIKAEV EEEMEEEEAE EEQVVEEEPT VKKKKKKDKK
510 520 530
KHIKEEPLSE EEPCTSTAVP SPEKKKKKKK KKDAED
Length:536
Mass (Da):60,343
Last modified:August 16, 2004 - v1
Checksum:iD5FE1819F092D701
GO

Sequence cautioni

The sequence AAC08435.1 differs from that shown. Reason: Frameshift at position 12. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781K → R in AAC08435 (Ref. 1) Curated
Sequence conflicti385 – 3851I → V in BAC31822 (PubMed:16141072).Curated
Sequence conflicti499 – 4991K → P in BAE22082 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053232 mRNA. Translation: AAC08435.1. Frameshift.
AK044216 mRNA. Translation: BAC31822.1.
AK133959 mRNA. Translation: BAE21954.1.
AK134286 mRNA. Translation: BAE22082.1.
BC076604 mRNA. Translation: AAH76604.1.
BC085135 mRNA. Translation: AAH85135.1.
CCDSiCCDS35587.1.
RefSeqiNP_061356.2. NM_018868.2.
UniGeneiMm.220367.

Genome annotation databases

EnsembliENSMUST00000191142; ENSMUSP00000140250; ENSMUSG00000026020.
GeneIDi55989.
KEGGimmu:55989.
UCSCiuc007bdy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053232 mRNA. Translation: AAC08435.1. Frameshift.
AK044216 mRNA. Translation: BAC31822.1.
AK133959 mRNA. Translation: BAE21954.1.
AK134286 mRNA. Translation: BAE22082.1.
BC076604 mRNA. Translation: AAH76604.1.
BC085135 mRNA. Translation: AAH85135.1.
CCDSiCCDS35587.1.
RefSeqiNP_061356.2. NM_018868.2.
UniGeneiMm.220367.

3D structure databases

ProteinModelPortaliQ6DFW4.
SMRiQ6DFW4. Positions 142-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207760. 1 interaction.
IntActiQ6DFW4. 4 interactions.
MINTiMINT-1854137.
STRINGi10090.ENSMUSP00000027174.

PTM databases

iPTMnetiQ6DFW4.
PhosphoSiteiQ6DFW4.

Proteomic databases

EPDiQ6DFW4.
MaxQBiQ6DFW4.
PaxDbiQ6DFW4.
PRIDEiQ6DFW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000191142; ENSMUSP00000140250; ENSMUSG00000026020.
GeneIDi55989.
KEGGimmu:55989.
UCSCiuc007bdy.1. mouse.

Organism-specific databases

CTDi51602.
MGIiMGI:1933184. Nop58.

Phylogenomic databases

eggNOGiKOG2572. Eukaryota.
COG1498. LUCA.
GeneTreeiENSGT00550000074998.
HOGENOMiHOG000196310.
InParanoidiQ6DFW4.
KOiK14565.
OMAiIRNQMEG.
OrthoDBiEOG7T7GT0.
PhylomeDBiQ6DFW4.
TreeFamiTF105688.

Enzyme and pathway databases

ReactomeiR-MMU-4570464. SUMOylation of RNA binding proteins.
R-MMU-6791226. Major pathway of rRNA processing in the nucleolus.

Miscellaneous databases

ChiTaRSiNop58. mouse.
PROiQ6DFW4.
SOURCEiSearch...

Gene expression databases

BgeeiQ6DFW4.
CleanExiMM_NOL5.
ExpressionAtlasiQ6DFW4. baseline and differential.
GenevisibleiQ6DFW4. MM.

Family and domain databases

InterProiIPR012974. NOP5_N.
IPR002687. Nop_dom.
IPR012976. NOSIC.
[Graphical view]
PfamiPF01798. Nop. 1 hit.
PF08156. NOP5NT. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA for mouse SIK similar protein (MSSP)."
    Zierke M., Martin M.U.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/HeJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Forelimb and Retina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Box C/D snoRNA-associated proteins: two pairs of evolutionarily ancient proteins and possible links to replication and transcription."
    Newman D.R., Kuhn J.F., Shanab G.M., Maxwell E.S.
    RNA 6:861-879(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH U14 BOX C/D SNORNPA.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509 AND SER-521, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509 AND SER-521, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509 AND SER-521, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiNOP58_MOUSE
AccessioniPrimary (citable) accession number: Q6DFW4
Secondary accession number(s): O70396
, Q3UYX9, Q3UZA3, Q8C8Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: August 16, 2004
Last modified: June 8, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.