Q6DFW4 (NOP58_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolar protein 58

Alternative name(s):
MSSP
Nucleolar protein 5
SIK-similar protein

Gene names

Name:	Nop58
Synonyms:	Nol5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for 60S ribosomal subunit biogenesis By similarity.
Subunit structure	Interacts with NOLC1/Nopp140. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Post-translational modification	Sumoylation is essential for high-affinity binding to snoRNAs By similarity.
Sequence similarities	Belongs to the NOP5/NOP56 family. Contains 1 Nop domain.
Sequence caution	The sequence AAC08435.1 differs from that shown. Reason: Frameshift at position 12.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Cellular component	Nucleus
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW snRNP protein import into nucleus Inferred from electronic annotation. Source: Compara
Cellular_component	Cajal body Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara nucleolus Inferred by curator PubMed 10209036. Source: MGI pre-snoRNP complex Inferred from electronic annotation. Source: Compara small nucleolar ribonucleoprotein complex Inferred from electronic annotation. Source: Compara
Molecular_function	snoRNA binding Inferred from electronic annotation. Source: Compara snoRNP binding Inferred from sequence orthology PubMed 10679015. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Nucleolar protein 58		PRO_0000287350
Regions
	Domain	282 – 400	119	Nop
	Compositional bias	441 – 532	92	Lys-rich
Amino acid modifications
	Modified residue	109	1	Phosphoserine By similarity
	Modified residue	351	1	Phosphoserine By similarity
	Modified residue	509	1	Phosphoserine Ref.4 Ref.5 Ref.6 Ref.7
	Modified residue	521	1	Phosphoserine Ref.4 Ref.5 Ref.6
	Cross-link	467		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	504		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Experimental info
	Sequence conflict	78	1	K → R in AAC08435. Ref.1
	Sequence conflict	385	1	I → V in BAC31822. Ref.2
	Sequence conflict	499	1	K → P in BAE22082. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6DFW4 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: D5FE1819F092D701	FASTA	536	60,343
	10 20 30 40 50 60 MLVLFETSVG YAIFKVLNEK KLQEVDSLWK EFETPEKANK IVKLKHFEKF QDTAEALAAF 70 80 90 100 110 120 TALMEGKINK QLKKVLKKIV KEAHEPLAVA DAKLGGVIKE KLNLSCIHSP VVNELMRGIR 130 140 150 160 170 180 SQMDGLIPGV EPREMAAMCL GLAHSLSRYR LKFSADKVDT MIVQAISLLD DLDKELNNYI 190 200 210 220 230 240 MRCREWYGWH FPELGKIISD NLTYCKCLQK VGDRKNYASA SLSEFLSEEV EAEVKAAAEI 250 260 270 280 290 300 SMGTEVSEED ICNILHLCTQ VIEISEYRTQ LYEYLQNRMM AIAPNVTVMV GELVGARLIA 310 320 330 340 350 360 HAGSLLNLAK HAASTVQILG AEKALFRALK SRRDTPKYGL IYHASLVGQS SPKHKGKISR 370 380 390 400 410 420 MLAAKTVLAI RYDAFGEDSS SAMGIENRAK LEARLRILED RGIRKISGTG KALAKAEKYE 430 440 450 460 470 480 HKSEVKTYDP SGDSTLPTCS KKRKIEEVDK EDEITEKKAK KAKIKIKAEV EEEMEEEEAE 490 500 510 520 530 EEQVVEEEPT VKKKKKKDKK KHIKEEPLSE EEPCTSTAVP SPEKKKKKKK KKDAED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA for mouse SIK similar protein (MSSP)." Zierke M., Martin M.U. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H/HeJ.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo, Forelimb and Retina.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509 AND SER-521, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509 AND SER-521, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509 AND SER-521, MASS SPECTROMETRY. Tissue: Melanoma.
[7]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-509, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF053232 mRNA. Translation: AAC08435.1. Frameshift. AK044216 mRNA. Translation: BAC31822.1. AK133959 mRNA. Translation: BAE21954.1. AK134286 mRNA. Translation: BAE22082.1. BC076604 mRNA. Translation: AAH76604.1. BC085135 mRNA. Translation: AAH85135.1.
IPI	IPI00463468.
RefSeq	NP_061356.2. NM_018868.2.
UniGene	Mm.220367.
3D structure databases
ProteinModelPortal	Q6DFW4.
SMR	Q6DFW4. Positions 142-401.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6DFW4. 1 interaction.
PTM databases
PhosphoSite	Q6DFW4.
Proteomic databases
PaxDb	Q6DFW4.
PRIDE	Q6DFW4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027174; ENSMUSP00000027174; ENSMUSG00000026020.
GeneID	55989.
KEGG	mmu:55989.
UCSC	uc007bdy.1. mouse.
Organism-specific databases
CTD	51602.
MGI	MGI:1933184. Nop58.
Phylogenomic databases
eggNOG	COG1498.
GeneTree	ENSGT00550000074998.
HOGENOM	HOG000196310.
InParanoid	Q6DFW4.
KO	K14565.
OMA	DAHEQLA.
OrthoDB	EOG451DQQ.
Gene expression databases
Bgee	Q6DFW4.
CleanEx	MM_NOL5.
Genevestigator	Q6DFW4.
Family and domain databases
InterPro	IPR012974. NOP5_N. IPR002687. Nop_dom. IPR012976. NOSIC. [Graphical view]
Pfam	PF01798. Nop. 1 hit. PF08156. NOP5NT. 1 hit. PF08060. NOSIC. 1 hit. [Graphical view]
SMART	SM00931. NOSIC. 1 hit. [Graphical view]
PROSITE	PS51358. NOP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NOP58. mouse.
NextBio	311710.
SOURCE	Search...

Entry information

Entry name

NOP58_MOUSE

Accession

Primary (citable) accession number: Q6DFW4
Secondary accession number(s): O70396 , Q3UYX9, Q3UZA3, Q8C8Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program