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Protein

E3 ubiquitin-protein ligase CBL-B

Gene

cblb

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei297PhosphotyrosineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi230 – 243PROSITE-ProRule annotationAdd BLAST14
Zinc fingeri384 – 423RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase CBL-B (EC:6.3.2.-)
Alternative name(s):
SH3-binding protein CBL-B
Signal transduction protein CBL-B
Gene namesi
Name:cblb
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-1018102. cblb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558651 – 982E3 ubiquitin-protein ligase CBL-BAdd BLAST982

Interactioni

Subunit structurei

Interacts with several SH3 domain-containing proteins and with poly-ubiquitinated proteins.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ6DFR2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 354Cbl-PTBPROSITE-ProRule annotationAdd BLAST309
Domaini927 – 970UBAPROSITE-ProRule annotationAdd BLAST44

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 1784HAdd BLAST133
Regioni179 – 251EF-hand-likeAdd BLAST73
Regioni252 – 354SH2-likeAdd BLAST103
Regioni355 – 383LinkerAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi487 – 576Pro-richAdd BLAST90
Compositional biasi683 – 688Poly-Pro6
Compositional biasi813 – 915Pro-richAdd BLAST103

Domaini

The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity
The UBA domain interacts with poly-ubiquitinated proteins.By similarity

Sequence similaritiesi

Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri384 – 423RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG005255.
InParanoidiQ6DFR2.
KOiK04707.

Family and domain databases

CDDicd09920. SH2_Cbl-b_TKB. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.20.930.20. 1 hit.
3.30.40.10. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR024162. Adaptor_Cbl.
IPR014741. Adaptor_Cbl_EF_hand-like.
IPR003153. Adaptor_Cbl_N_hlx.
IPR014742. Adaptor_Cbl_SH2-like.
IPR024159. Cbl_PTB.
IPR011992. EF-hand-dom_pair.
IPR000980. SH2.
IPR015940. UBA.
IPR009060. UBA-like.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR23007. PTHR23007. 2 hits.
PfamiPF02262. Cbl_N. 1 hit.
PF02761. Cbl_N2. 1 hit.
PF02762. Cbl_N3. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF47473. SSF47473. 1 hit.
SSF47668. SSF47668. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS51506. CBL_PTB. 1 hit.
PS50030. UBA. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DFR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSSSSSS TNSSAVTGRL PGARSANPRK ARILGLFDAI QDAVGPPKQA
60 70 80 90 100
AADRRTVEKT WKLMDKVVRL CQNPKLQLKN SPPYILDILP DTYQHLRLIL
110 120 130 140 150
SKYDDNQKLA QLSENEYFKI YIDSLMKKSK RAIRLFKEGK ERMYEEQSQE
160 170 180 190 200
RRNLTKLSLI FSHMLAEIKA IFPSGQFQGD NFRITKADAA EFWRKFFGER
210 220 230 240 250
TIVPWKIFRQ CLHEVHQISS GLEAMALKST IDLTCNDYIS VFEFDIFTRL
260 270 280 290 300
FQPWTSILRN WNFLAVTHPG YMAFLTYDEV KARLQKYSTK PGSYIFRLSC
310 320 330 340 350
TRLGQWAIGY VTADGNILQT IPHNKPLFQA LIDGSREGFY LYPDGRSYNP
360 370 380 390 400
DLTDLCEPTP HDHIKVTQEQ YELYCEMGST FQLCKICAEN DKDVKIEPCG
410 420 430 440 450
HLMCTSCLTS WQESDGQGCP FCRCEIKGTE PIVVDPFDPR DENRCCSFND
460 470 480 490 500
SLCTPMLDFD DDDLREECLI MNRLASLRKM NERQNSPVTS PGSSPLSQRR
510 520 530 540 550
KTPPDPLQIP HLNLPPVPPR LDLIQKGLAR SPCASPTGSP KSSPCMVRKQ
560 570 580 590 600
DKPLPAPPPP LREPPPPPER PPPIPPDSRT CRHLHHTENV PCRDQSTQHD
610 620 630 640 650
AWCTRDISGA SQPSICRVAH DGSPKLGVPS SSVLNGRHSR MSTEAGFIRH
660 670 680 690 700
KHHKRRESPL ETIRVYNGLS GNEEYDVPPR LSPPPPPPTI TIHPAIKCPL
710 720 730 740 750
LVNSVSDKVR NSAEEDDSEY KIPSSHPVSS RLPLHCHSIK HFPRLCENGQ
760 770 780 790 800
CLSNGTHNGI SEIKKLKQPD QGDVIATSTV PVPLPSARTS ARENHPHGSS
810 820 830 840 850
LTRTPSDYDL LVPHPGEESF DSSPPSQPPP PPPARTCVPE HAMPTASGCR
860 870 880 890 900
PNSDVDLFLP HSDPCPEAPL PPARRGPGEA KSNRLSQEYD QLPSCPDCPQ
910 920 930 940 950
APARPPKPVP RRTAPEIHHR RHYNCDSLAE NVDAKIAKLM GEGFPFEEVK
960 970 980
RALEIAQNNV DVARSILREF AFPPPVCPRL HL
Length:982
Mass (Da):110,118
Last modified:August 16, 2004 - v1
Checksum:iB55D0D9C0F0658A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC076671 mRNA. Translation: AAH76671.1.
RefSeqiNP_001006802.1. NM_001006801.1.
UniGeneiStr.5685.

Genome annotation databases

GeneIDi448509.
KEGGixtr:448509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC076671 mRNA. Translation: AAH76671.1.
RefSeqiNP_001006802.1. NM_001006801.1.
UniGeneiStr.5685.

3D structure databases

ProteinModelPortaliQ6DFR2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi448509.
KEGGixtr:448509.

Organism-specific databases

CTDi868.
XenbaseiXB-GENE-1018102. cblb.

Phylogenomic databases

HOVERGENiHBG005255.
InParanoidiQ6DFR2.
KOiK04707.

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

CDDicd09920. SH2_Cbl-b_TKB. 1 hit.
Gene3Di1.10.238.10. 1 hit.
1.20.930.20. 1 hit.
3.30.40.10. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR024162. Adaptor_Cbl.
IPR014741. Adaptor_Cbl_EF_hand-like.
IPR003153. Adaptor_Cbl_N_hlx.
IPR014742. Adaptor_Cbl_SH2-like.
IPR024159. Cbl_PTB.
IPR011992. EF-hand-dom_pair.
IPR000980. SH2.
IPR015940. UBA.
IPR009060. UBA-like.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR23007. PTHR23007. 2 hits.
PfamiPF02262. Cbl_N. 1 hit.
PF02761. Cbl_N2. 1 hit.
PF02762. Cbl_N3. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF47473. SSF47473. 1 hit.
SSF47668. SSF47668. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS51506. CBL_PTB. 1 hit.
PS50030. UBA. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBLB_XENTR
AccessioniPrimary (citable) accession number: Q6DFR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: August 16, 2004
Last modified: November 30, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has one functional calcium-binding site.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.