Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q6DFR2 (CBLB_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase CBL-B

EC=6.3.2.-
Alternative name(s):
SH3-binding protein CBL-B
Signal transduction protein CBL-B
Gene names
Name:cblb
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length982 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with several SH3 domain-containing proteins and with poly-ubiquitinated proteins By similarity.

Subcellular location

Cytoplasm By similarity.

Domain

The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.

The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme By similarity.

The UBA domain interacts with poly-ubiquitinated proteins By similarity.

Miscellaneous

This protein has one functional calcium-binding site By similarity.

Sequence similarities

Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.

Contains 1 RING-type zinc finger.

Contains 1 UBA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 982982E3 ubiquitin-protein ligase CBL-B
PRO_0000055865

Regions

Domain46 – 354309Cbl-PTB
Domain927 – 97044UBA
Calcium binding230 – 24314 By similarity
Zinc finger384 – 42340RING-type
Region46 – 1781334H
Region179 – 25173EF-hand-like
Region252 – 354103SH2-like
Region355 – 38329Linker
Compositional bias487 – 57690Pro-rich
Compositional bias683 – 6886Poly-Pro
Compositional bias813 – 915103Pro-rich

Sites

Binding site2971Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6DFR2 [UniParc].

Last modified August 16, 2004. Version 1.
Checksum: B55D0D9C0F0658A1

FASTA982110,118
        10         20         30         40         50         60 
MASSSSSSSS TNSSAVTGRL PGARSANPRK ARILGLFDAI QDAVGPPKQA AADRRTVEKT 

        70         80         90        100        110        120 
WKLMDKVVRL CQNPKLQLKN SPPYILDILP DTYQHLRLIL SKYDDNQKLA QLSENEYFKI 

       130        140        150        160        170        180 
YIDSLMKKSK RAIRLFKEGK ERMYEEQSQE RRNLTKLSLI FSHMLAEIKA IFPSGQFQGD 

       190        200        210        220        230        240 
NFRITKADAA EFWRKFFGER TIVPWKIFRQ CLHEVHQISS GLEAMALKST IDLTCNDYIS 

       250        260        270        280        290        300 
VFEFDIFTRL FQPWTSILRN WNFLAVTHPG YMAFLTYDEV KARLQKYSTK PGSYIFRLSC 

       310        320        330        340        350        360 
TRLGQWAIGY VTADGNILQT IPHNKPLFQA LIDGSREGFY LYPDGRSYNP DLTDLCEPTP 

       370        380        390        400        410        420 
HDHIKVTQEQ YELYCEMGST FQLCKICAEN DKDVKIEPCG HLMCTSCLTS WQESDGQGCP 

       430        440        450        460        470        480 
FCRCEIKGTE PIVVDPFDPR DENRCCSFND SLCTPMLDFD DDDLREECLI MNRLASLRKM 

       490        500        510        520        530        540 
NERQNSPVTS PGSSPLSQRR KTPPDPLQIP HLNLPPVPPR LDLIQKGLAR SPCASPTGSP 

       550        560        570        580        590        600 
KSSPCMVRKQ DKPLPAPPPP LREPPPPPER PPPIPPDSRT CRHLHHTENV PCRDQSTQHD 

       610        620        630        640        650        660 
AWCTRDISGA SQPSICRVAH DGSPKLGVPS SSVLNGRHSR MSTEAGFIRH KHHKRRESPL 

       670        680        690        700        710        720 
ETIRVYNGLS GNEEYDVPPR LSPPPPPPTI TIHPAIKCPL LVNSVSDKVR NSAEEDDSEY 

       730        740        750        760        770        780 
KIPSSHPVSS RLPLHCHSIK HFPRLCENGQ CLSNGTHNGI SEIKKLKQPD QGDVIATSTV 

       790        800        810        820        830        840 
PVPLPSARTS ARENHPHGSS LTRTPSDYDL LVPHPGEESF DSSPPSQPPP PPPARTCVPE 

       850        860        870        880        890        900 
HAMPTASGCR PNSDVDLFLP HSDPCPEAPL PPARRGPGEA KSNRLSQEYD QLPSCPDCPQ 

       910        920        930        940        950        960 
APARPPKPVP RRTAPEIHHR RHYNCDSLAE NVDAKIAKLM GEGFPFEEVK RALEIAQNNV 

       970        980 
DVARSILREF AFPPPVCPRL HL 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC076671 mRNA. Translation: AAH76671.1.
RefSeqNP_001006802.1. NM_001006801.1.
UniGeneStr.5685.

3D structure databases

ProteinModelPortalQ6DFR2.
SMRQ6DFR2. Positions 46-437, 930-972.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8364.ENSXETP00000035178.

Proteomic databases

PRIDEQ6DFR2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID448509.
KEGGxtr:448509.

Organism-specific databases

CTD868.
XenbaseXB-GENE-1018102. cblb.

Phylogenomic databases

eggNOGNOG242251.
HOVERGENHBG005255.
KOK04707.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
1.20.930.20. 1 hit.
3.30.40.10. 1 hit.
3.30.505.10. 1 hit.
InterProIPR024162. Adaptor_Cbl.
IPR014741. Adaptor_Cbl_EF_hand-like.
IPR003153. Adaptor_Cbl_N_hlx.
IPR014742. Adaptor_Cbl_SH2-like.
IPR024159. Cbl_PTB.
IPR011992. EF-hand-dom_pair.
IPR000980. SH2.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERPTHR23007. PTHR23007. 1 hit.
PfamPF02262. Cbl_N. 1 hit.
PF02761. Cbl_N2. 1 hit.
PF02762. Cbl_N3. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
SM00252. SH2. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
SSF47668. SSF47668. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEPS51506. CBL_PTB. 1 hit.
PS50030. UBA. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCBLB_XENTR
AccessionPrimary (citable) accession number: Q6DFR2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: August 16, 2004
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways