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Q6DFA8

- GLD2B_XENLA

UniProt

Q6DFA8 - GLD2B_XENLA

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Protein

Poly(A) RNA polymerase GLD2-B

Gene

papd4-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs during oocyte maturation. Plays a central role during oocyte maturation by mediating polyadenylation of dormant mRNAs, which contain 5'AAUAAA-3' sequence in their 3'-UTR. In immature oocytes, polyadenylation of poly(A) tails is counteracted by the ribonuclease parn. During maturation parn is excluded from the ribonucleoprotein complex, allowing poly(A) elongation and activation of mRNAs. May not play a role in replication-dependent histone mRNA degradation (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi240 – 2401Magnesium or manganese; catalyticBy similarity
Metal bindingi242 – 2421Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
  3. oogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Transferase

Keywords - Biological processi

Differentiation, mRNA processing, Oogenesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase GLD2-B (EC:2.7.7.19)
Short name:
xGLD-2
Alternative name(s):
PAP-associated domain-containing protein 4-B
Gene namesi
Name:papd4-b
Synonyms:gld2-b
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6256236. papd4.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi242 – 2421D → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Poly(A) RNA polymerase GLD2-BPRO_0000341554Add
BLAST

Proteomic databases

PRIDEiQ6DFA8.

Expressioni

Developmental stagei

Present in oocytes.1 Publication

Inductioni

Autoregulated; mediates its own polyadenylation and translational activation during frog oocyte maturation.1 Publication

Interactioni

Subunit structurei

Component of a complex at least composed of cpeb1, cpsf1, papd4/gld2, pabpc1/ePAB, parn and sympk. Following oocyte maturation, parn is expelled from the complex. Interacts with rbfox2. Interacts with sympk (By similarity).By similarity

Protein-protein interaction databases

IntActiQ6DFA8. 1 interaction.
MINTiMINT-7983711.

Structurei

3D structure databases

ProteinModelPortaliQ6DFA8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini409 – 46254PAP-associatedAdd
BLAST

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

HOVERGENiHBG097533.
KOiK14079.

Family and domain databases

InterProiIPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q6DFA8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYPNSPSLGR IPLPLPCARQ QQTSAYLSKV PVSVAPDLLS PEQFFQASLN
60 70 80 90 100
IHKNANLPRL LMNGNVLTVP PLSSPPWSYL NHSPLISPGS PPSSFQNRKR
110 120 130 140 150
RSDEGNSPYD VKRQRFQSPQ EQTVNHQAVP LRGDIRCSYP GSPAFPLLQS
160 170 180 190 200
PSPPVLKGHS SNSGDCWLYD HIDTTLPVAK DKLSKQILDL FQALQQQVCD
210 220 230 240 250
LKKKDICRAE LQREIQQIFP QSRLYLVGSS LNGFGIRSSD ADLCLVLKEE
260 270 280 290 300
PMNQNTEARH ILSLLHKHFY TRLSYIERPQ FIRAKVPIVK FRDKVSGAEF
310 320 330 340 350
DLNVNNVVGI RNTFLLRTYA YLDKRVRPLV LVIKKWANHH GINDASRGTL
360 370 380 390 400
SSYTIVLMVL HYLQTLPEPI LPSLQKKYPE CFDRTMQLHL VHQAPRNIPQ
410 420 430 440 450
FLSKNETPLG DLLLGFLKYF AVEFDWSKDI ISLREAKALP RTDDYEWRNK
460 470 480 490 500
YICVEEPFDG SNTARAVYEK QKFDLIRAEF LKAWVALRDN RDLYSLLPVK

GIMKKMHSL
Length:509
Mass (Da):58,235
Last modified:August 16, 2004 - v1
Checksum:i8FB3569E258252D7
GO
Isoform 2 (identifier: Q6DFA8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-98: Missing.

Show »
Length:466
Mass (Da):53,570
Checksum:i51E6DD2192BAEAB8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281S → T in AAT98005. (PubMed:15550246)Curated
Sequence conflicti133 – 1331G → S in AAT98005. (PubMed:15550246)Curated
Sequence conflicti376 – 3761K → R in AAT98005. (PubMed:15550246)Curated
Sequence conflicti430 – 4301I → V in AAT98005. (PubMed:15550246)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 9843Missing in isoform 2. 1 PublicationVSP_034326Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY655140 mRNA. Translation: AAT98005.1.
AM419010 mRNA. Translation: CAL91353.1.
BC076832 mRNA. Translation: AAH76832.1.
RefSeqiNP_001086580.1. NM_001093111.1. [Q6DFA8-1]
NP_001087078.1. NM_001093609.1.
UniGeneiXl.63197.
Xl.75862.

Genome annotation databases

GeneIDi446415.
446914.
KEGGixla:446415.
xla:446914.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY655140 mRNA. Translation: AAT98005.1 .
AM419010 mRNA. Translation: CAL91353.1 .
BC076832 mRNA. Translation: AAH76832.1 .
RefSeqi NP_001086580.1. NM_001093111.1. [Q6DFA8-1 ]
NP_001087078.1. NM_001093609.1.
UniGenei Xl.63197.
Xl.75862.

3D structure databases

ProteinModelPortali Q6DFA8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q6DFA8. 1 interaction.
MINTi MINT-7983711.

Proteomic databases

PRIDEi Q6DFA8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 446415.
446914.
KEGGi xla:446415.
xla:446914.

Organism-specific databases

CTDi 446415.
446914.
Xenbasei XB-GENE-6256236. papd4.

Phylogenomic databases

HOVERGENi HBG097533.
KOi K14079.

Family and domain databases

InterProi IPR002934. Nucleotidyltransferase.
IPR002058. PAP_assoc.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF03828. PAP_assoc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation."
    Barnard D.C., Ryan K., Manley J.L., Richter J.D.
    Cell 119:641-651(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CPEB1; CPSF1 AND SYMPK, MUTAGENESIS OF ASP-242.
    Tissue: Oocyte.
  2. "Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex."
    Papin C., Rouget C., Mandart E.
    FEBS J. 275:490-503(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH RBFOX2.
    Tissue: Oocyte.
  3. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Oocyte.
  4. "Vertebrate GLD2 poly(A) polymerases in the germline and the brain."
    Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M.
    RNA 11:1117-1130(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  5. "Opposing polymerase-deadenylase activities regulate cytoplasmic polyadenylation."
    Kim J.H., Richter J.D.
    Mol. Cell 24:173-183(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PARN.
  6. "RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB."
    Kim J.H., Richter J.D.
    Genes Dev. 21:2571-2579(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PABPC1.
  7. "Autoregulation of GLD-2 cytoplasmic poly(A) polymerase."
    Rouhana L., Wickens M.
    RNA 13:188-199(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiGLD2B_XENLA
AccessioniPrimary (citable) accession number: Q6DFA8
Secondary accession number(s): A4F5G9, Q68PF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: August 16, 2004
Last modified: November 26, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3