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Protein

Calcium/calmodulin-dependent protein kinase type II delta 1 chain

Gene

camk2d1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.Curated

Enzyme regulationi

Autophosphorylation of CAMK2 plays an important role in the regulation of the kinase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATPPROSITE-ProRule annotation
Active sitei135 – 1351Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin-dependent protein kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_207863. CREB phosphorylation through the activation of Ras.
REACT_211877. Unblocking of NMDA receptor, glutamate binding and activation.
REACT_219102. HSF1-dependent transactivation.
REACT_221995. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_223489. CREB phosphorylation through the activation of CaMKII.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase type II delta 1 chain1 Publication (EC:2.7.11.17)
Alternative name(s):
Calcium/calmodulin-dependent protein kinase type II delta-B chain
Short name:
CaM kinase II subunit delta-B
Short name:
CaM-kinase II delta-B chain
Short name:
CaMK-II subunit delta-B
Gene namesi
Name:camk2d11 Publication
Synonyms:camk2dbImported
ORF Names:zgc:101001, zgc:173815
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 7

Organism-specific databases

ZFINiZDB-GENE-040801-121. camk2d1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Calcium/calmodulin-dependent protein kinase type II delta 1 chainPRO_0000296340Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei286 – 2861PhosphothreonineBy similarity
Modified residuei314 – 3141PhosphoserineBy similarity
Modified residuei350 – 3501PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ6DEH3.

Expressioni

Tissue specificityi

First detected at the 18-somite stage where expression is restricted to somite boundaries. At 24 hpf, expression is elevated in epidermal tissue and in the hatching gland. After 24 hpf, expression dimishes, but persists at low levels along the dorsal trunk. At 48 hpf, expression is restricted at a low level to the forebrain. At 72 hpf, weak expression reappears along the entire dorsal trunk in discrete cell bodies.1 Publication

Gene expression databases

BgeeiQ6DEH3.

Interactioni

Subunit structurei

CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6DEH3.
SMRiQ6DEH3. Positions 10-308, 349-487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 271259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ6DEH3.
KOiK04515.
OMAiFSSKSIM.
OrthoDBiEOG7ZD1VM.
PhylomeDBiQ6DEH3.
TreeFamiTF315229.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR013543. Ca/CaM-dep_prot_kinase-assoc.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF08332. CaMKII_AD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform E1 Publication (identifier: Q6DEH3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTTCTRFT DEYQLYEELG KGAFSVVRRC MKISTGQEYA AKIINTKKLS
60 70 80 90 100
ARDHQKLERE ARICRLLKHA NIVRLHDSIS EEGVHYLVFD LVTGGELFED
110 120 130 140 150
IVAREYYSEA DASHCIQQIL EAVLHCHQMG VVHRDLKPEN LLLASKLKGA
160 170 180 190 200
AVKLADFGLA IEVQGDQQAW FGFAGTPGYL SPEVLRKEPY GKPVDMWACG
210 220 230 240 250
VILYILLVGY PPFWDEDQHR LYQQIKAGAY DFPSPEWDTV TPEAKDLINK
260 270 280 290 300
MLTINPAKRI TAAEALKHPW ICQRSTVASM MHRQETVECL KKFNARRKLK
310 320 330 340 350
GAILTTMLAT RNFSSKNPYK KPDGVKEPQT TVIHNPTDGN KESSESTNTT
360 370 380 390 400
IEDEDIKARK QEIIKVTELL IEAINNGEFE AYTKICDPGL TSFEPEALGN
410 420 430 440 450
LVEGTDFHRF YFENSLSKGH KPIHTILLNP HVHLIGEDAA CIAYIRLTQY
460 470 480 490
MDVNNMPRTM QSEETRVWHR RDGKWQNIHF HRSGSPTVPT K
Length:491
Mass (Da):55,829
Last modified:October 14, 2008 - v2
Checksum:iADCFD717E279AF15
GO
Isoform G1 Publication (identifier: Q6DEH3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     326-340: Missing.

Show »
Length:476
Mass (Da):54,196
Checksum:i7FE8293D631CD611
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei326 – 34015Missing in isoform G. 2 PublicationsVSP_035558Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC077143 mRNA. Translation: AAH77143.1.
BC154189 mRNA. Translation: AAI54190.1.
RefSeqiNP_001003602.1. NM_001003602.2. [Q6DEH3-2]
NP_001108180.1. NM_001114708.2. [Q6DEH3-1]
UniGeneiDr.78101.

Genome annotation databases

EnsembliENSDART00000056466; ENSDARP00000056465; ENSDARG00000043010. [Q6DEH3-1]
ENSDART00000142259; ENSDARP00000119349; ENSDARG00000043010. [Q6DEH3-2]
GeneIDi445208.
KEGGidre:445208.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC077143 mRNA. Translation: AAH77143.1.
BC154189 mRNA. Translation: AAI54190.1.
RefSeqiNP_001003602.1. NM_001003602.2. [Q6DEH3-2]
NP_001108180.1. NM_001114708.2. [Q6DEH3-1]
UniGeneiDr.78101.

3D structure databases

ProteinModelPortaliQ6DEH3.
SMRiQ6DEH3. Positions 10-308, 349-487.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ6DEH3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000056466; ENSDARP00000056465; ENSDARG00000043010. [Q6DEH3-1]
ENSDART00000142259; ENSDARP00000119349; ENSDARG00000043010. [Q6DEH3-2]
GeneIDi445208.
KEGGidre:445208.

Organism-specific databases

CTDi445208.
ZFINiZDB-GENE-040801-121. camk2d1.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ6DEH3.
KOiK04515.
OMAiFSSKSIM.
OrthoDBiEOG7ZD1VM.
PhylomeDBiQ6DEH3.
TreeFamiTF315229.

Enzyme and pathway databases

ReactomeiREACT_207863. CREB phosphorylation through the activation of Ras.
REACT_211877. Unblocking of NMDA receptor, glutamate binding and activation.
REACT_219102. HSF1-dependent transactivation.
REACT_221995. Ras activation uopn Ca2+ infux through NMDA receptor.
REACT_223489. CREB phosphorylation through the activation of CaMKII.

Miscellaneous databases

NextBioi20831969.

Gene expression databases

BgeeiQ6DEH3.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR013543. Ca/CaM-dep_prot_kinase-assoc.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF08332. CaMKII_AD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS E AND G).
    Tissue: EmbryoImported and Olfactory epitheliumImported.
  2. "Differential expression of CaMK-II genes during early zebrafish embryogenesis."
    Rothschild S.C., Lister J.A., Tombes R.M.
    Dev. Dyn. 236:295-305(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 301-383 (ISOFORMS E AND G), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.

Entry informationi

Entry nameiKC2D1_DANRE
AccessioniPrimary (citable) accession number: Q6DEH3
Secondary accession number(s): A8KBP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: October 14, 2008
Last modified: January 7, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.