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Protein

Aurora kinase B-A

Gene

aurkb-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Phosphorylates 'Ser-10' of histone H3 during mitosis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+By similarity

Enzyme regulationi

Kinase activity is stimulated by both birc5/survivin-binding and cell-cycle specific phosphorylation.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221ATPPROSITE-ProRule annotation
Active sitei216 – 2161Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 1079ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aurora kinase B-A (EC:2.7.11.1)
Alternative name(s):
Aurora/IPL1-related kinase 2-A
Short name:
AIRK2-A
Short name:
XAIRK2-A
Serine/threonine-protein kinase 12-A
Serine/threonine-protein kinase aurora-B-A
Short name:
xAurora-B
Gene namesi
Name:aurkb-a
Synonyms:airk2-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1019634. aurkb.

Subcellular locationi

  • Nucleus
  • Chromosome

  • Note: Chromosomal until metaphase but transfers to the midzone microtubule array and the equatorial cortex during anaphase.

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • chromosome Source: UniProtKB
  • chromosome, centromeric region Source: UniProtKB
  • chromosome passenger complex Source: UniProtKB
  • midbody Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2176838.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 361361Aurora kinase B-APRO_0000281015Add
BLAST

Post-translational modificationi

Phosphorylated, stimulates kinase activity.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Component of the CPC at least composed of survivin/birc5, incenp, cdca8/borealin and/or cdca9/dasra-A, and aurkb/aurora-B. Interacts directly (via N-terminus and kinase domain) with incenp (via C terminus), and may weakly interact (via N-terminus) with birc5.1 to stabilize the complex. Interacts with mtus1.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
incenp-aO130242EBI-1042262,EBI-1042275

Protein-protein interaction databases

BioGridi99789. 3 interactions.
IntActiQ6DE08. 3 interactions.

Chemistry

BindingDBiQ6DE08.

Structurei

Secondary structure

1
361
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni78 – 803Combined sources
Helixi90 – 923Combined sources
Beta strandi93 – 1008Combined sources
Turni103 – 1053Combined sources
Beta strandi106 – 1127Combined sources
Turni113 – 1164Combined sources
Beta strandi117 – 1259Combined sources
Helixi126 – 1316Combined sources
Turni132 – 1343Combined sources
Helixi135 – 14612Combined sources
Beta strandi156 – 1616Combined sources
Beta strandi163 – 1708Combined sources
Helixi178 – 1858Combined sources
Helixi190 – 20819Combined sources
Turni209 – 2113Combined sources
Helixi219 – 2213Combined sources
Beta strandi222 – 2243Combined sources
Beta strandi230 – 2323Combined sources
Beta strandi242 – 2443Combined sources
Helixi253 – 2553Combined sources
Helixi258 – 2614Combined sources
Helixi270 – 28415Combined sources
Helixi294 – 3029Combined sources
Helixi314 – 32310Combined sources
Helixi328 – 3303Combined sources
Helixi334 – 3385Combined sources
Helixi341 – 3466Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BFXX-ray1.80A/B78-361[»]
2BFYX-ray1.80A/B78-361[»]
2VGOX-ray1.70A/B78-361[»]
2VGPX-ray1.70A/B78-361[»]
2VRXX-ray1.86A/B77-361[»]
3ZTXX-ray1.95A/B78-361[»]
4B8LX-ray3.00A78-361[»]
4B8MX-ray1.85A/B78-361[»]
4C2VX-ray1.49A/B76-360[»]
4C2WX-ray1.70A/B78-356[»]
ProteinModelPortaliQ6DE08.
SMRiQ6DE08. Positions 78-355.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6DE08.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini93 – 343251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG108519.
KOiK11479.

Family and domain databases

InterProiIPR030616. Aur.
IPR028772. AURKB.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 2 hits.
PTHR24350:SF4. PTHR24350:SF4. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6DE08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYKENLNPS SYTSKFTTPS SATAAQRVLR KEPYVSTFTT PSDNLLAQRT
60 70 80 90 100
QLSRITPSAS SSVPGRVAVS TEMPSQNTAL AEMPKRKFTI DDFDIGRPLG
110 120 130 140 150
KGKFGNVYLA REKQNKFIMA LKVLFKSQLE KEGVEHQLRR EIEIQSHLRH
160 170 180 190 200
PNILRMYNYF HDRKRIYLML EFAPRGELYK ELQKHGRFDE QRSATFMEEL
210 220 230 240 250
ADALHYCHER KVIHRDIKPE NLLMGYKGEL KIADFGWSVH APSLRRRTMC
260 270 280 290 300
GTLDYLPPEM IEGKTHDEKV DLWCAGVLCY EFLVGMPPFD SPSHTETHRR
310 320 330 340 350
IVNVDLKFPP FLSDGSKDLI SKLLRYHPPQ RLPLKGVMEH PWVKANSRRV
360
LPPVYQSTQS K
Length:361
Mass (Da):41,735
Last modified:August 16, 2004 - v1
Checksum:i384803072040015B
GO

Sequence cautioni

The sequence AAH41288 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → E in AAG10787 (PubMed:10996078).Curated
Sequence conflicti2 – 21S → E in AAM76715 (PubMed:12221116).Curated
Sequence conflicti87 – 871K → Q in AAG10787 (PubMed:10996078).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292096 mRNA. Translation: AAG10787.1.
AY115554 mRNA. Translation: AAM76715.1.
BC041288 mRNA. Translation: AAH41288.1. Different initiation.
BC077339 mRNA. Translation: AAH77339.1.
RefSeqiNP_001082418.1. NM_001088949.1.
UniGeneiXl.6652.

Genome annotation databases

GeneIDi398457.
KEGGixla:398457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF292096 mRNA. Translation: AAG10787.1.
AY115554 mRNA. Translation: AAM76715.1.
BC041288 mRNA. Translation: AAH41288.1. Different initiation.
BC077339 mRNA. Translation: AAH77339.1.
RefSeqiNP_001082418.1. NM_001088949.1.
UniGeneiXl.6652.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BFXX-ray1.80A/B78-361[»]
2BFYX-ray1.80A/B78-361[»]
2VGOX-ray1.70A/B78-361[»]
2VGPX-ray1.70A/B78-361[»]
2VRXX-ray1.86A/B77-361[»]
3ZTXX-ray1.95A/B78-361[»]
4B8LX-ray3.00A78-361[»]
4B8MX-ray1.85A/B78-361[»]
4C2VX-ray1.49A/B76-360[»]
4C2WX-ray1.70A/B78-356[»]
ProteinModelPortaliQ6DE08.
SMRiQ6DE08. Positions 78-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi99789. 3 interactions.
IntActiQ6DE08. 3 interactions.

Chemistry

BindingDBiQ6DE08.
ChEMBLiCHEMBL2176838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi398457.
KEGGixla:398457.

Organism-specific databases

CTDi398457.
XenbaseiXB-GENE-1019634. aurkb.

Phylogenomic databases

HOVERGENiHBG108519.
KOiK11479.

Miscellaneous databases

EvolutionaryTraceiQ6DE08.
PROiQ6DE08.

Family and domain databases

InterProiIPR030616. Aur.
IPR028772. AURKB.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 2 hits.
PTHR24350:SF4. PTHR24350:SF4. 2 hits.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAUKBA_XENLA
AccessioniPrimary (citable) accession number: Q6DE08
Secondary accession number(s): Q7ZYT9, Q8JG74, Q9DF70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: August 16, 2004
Last modified: June 8, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.