Q6DDV8 (TPX2B_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Targeting protein for Xklp2-B

Gene names

Name:

tpx2-b

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Spindle assembly factor. Required for normal assembly of mitotic spindles. Mediates the binding kif15 and aurka to spindle microtubules. Required for targeting kif15 to microtubule minus ends. Activates aurka by promoting its autophosphorylation and protects the phosphorylated residue against dephosphorylation By similarity.
Subunit structure	Associates with microtubules. Interacts with aurka and plk1. Interacts with kif15 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity. Note: Localizes during metaphase on spindle microtubules, with a strong enrichment at spindle poles. Localizes to the minus ends of spindle pole and aster microtubules in a dynein- and dynactin-dependent manner By similarity.
Post-translational modification	Phosphorylated during mitosis. Hyperphosphorylated upon assembly of microtubules By similarity.
Sequence similarities	Belongs to the TPX2 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule Nucleus
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	Targeting protein for Xklp2-B		PRO_0000393115
Amino acid modifications
	Modified residue	204	1	Phosphoserine; by plk1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DDV8 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 1BA4194FB09FD164	FASTA	715	82,010
	10 20 30 40 50 60 MADAQDTYSY DAPSIFNFSS FHDDHNADSW FDQLTNAENI PPDQKPLSET SVNAEQNCKV 70 80 90 100 110 120 EPGKITPSKE EVSKSTTHVC DVKSQTKRSA RRMSKKHRQK ILLKMKETHL EKETAQSEYP 130 140 150 160 170 180 PCKKLKGSST KDRQAPVIRG QPTSSHHGTT SPKPKAQLTM PATPTVLKRR NILAKSKSSE 190 200 210 220 230 240 EQELEKMQAL QKEMLENLKK NEHSMKVAIS GAGQPVKNFI PVTKPVDFHF KTDDRLKRNA 250 260 270 280 290 300 NPPEGDGYKE VDFASVLRKP PTSPVQVTKG GHTVPKPFNL SKGKRKHEEA SDFVSTAEQV 310 320 330 340 350 360 VAFYRRTPAR YHLRSRQKEM EGPSPVKMLK PKLTNPKTPL LQTKQRHRPT TCKSAAELEA 370 380 390 400 410 420 EELDMIHQYK FKAQELDTRI LEGGPVLPKK PSVKEPTKAI GFDLEIEKRI QQREKKDEVE 430 440 450 460 470 480 EEAFSFHSRP CPSKILADVV GVPQKKLLPV TVPQSPAFAL KNRVRIPAQE EKEEEVPVIK 490 500 510 520 530 540 ATRMPHYGVP FKPKLVEQKQ VEACPFSFCE RDKERQLQKE KRLDELRKEE VPKFKAQRLP 550 560 570 580 590 600 QFDHISLPEK KVKMPTQQEP FQLEIDKRGA TKLQRWQHQI KEELKQQKEM VVFKARPNTV 610 620 630 640 650 660 VHQEPFVPKK ESRSLTDSLS GSMIQEGFEL ATAKRAKERQ EFEKCLAEME AQKSLLEEET 670 680 690 700 710 RKQREEEERE EINQLRQELV HKAQPIRKFK AVEVKASDVP LTVPASPNFS DRFKC

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Gastrula.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC077396 mRNA. Translation: AAH77396.1.
RefSeq	NP_001086751.1. NM_001093282.1.
UniGene	Xl.4592.
3D structure databases
ProteinModelPortal	Q6DDV8.
SMR	Q6DDV8. Positions 7-39.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	446586.
KEGG	xla:446586.
Organism-specific databases
CTD	446586.
Xenbase	XB-GENE-6255926. tpx2.
Phylogenomic databases
HOVERGEN	HBG057334.
Family and domain databases
InterPro	IPR015128. Aurora-A-bd. IPR022021. TPX2_importin. IPR009675. Xklp2_targeting_prot. [Graphical view]
Pfam	PF09041. Aurora-A_bind. 1 hit. PF06886. TPX2. 1 hit. PF12214. TPX2_importin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPX2B_XENLA

Accession

Primary (citable) accession number: Q6DDV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	August 16, 2004
Last modified:	November 30, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program