Reviewed, UniProtKB/Swiss-Prot Q6DC04 (CARM1_DANRE)

Names and origin

Protein names

Recommended name:
    Histone-arginine methyltransferase CARM1
    EC=2.1.1.125
    EC=2.1.1.-
Alternative name(s):
    Protein arginine N-methyltransferase 4
    Coactivator-associated arginine methyltransferase 1

Gene names

Name:	carm1
ORF Names:	si:dkey-204f11.63, zgc:100805

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone-arginine = S-adenosyl-L-homocysteine + histone-N(omega)-methyl-arginine.
Subunit structure	Homodimer By similarity.
Subcellular location	Nucleus. Cytoplasm By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid methylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-arginine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Histone-arginine methyltransferase CARM1		PRO_0000249251
Regions
	Region	473 – 588	116	Transactivation domain By similarity
Sites
	Binding site	133	1	S-adenosyl-L-methionine By similarity
	Binding site	142	1	S-adenosyl-L-methionine By similarity
	Binding site	166	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	188	1	S-adenosyl-L-methionine By similarity
	Binding site	217	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DC04-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 410AB00B486DBAD7	FASTA	588	64,896
	10 20 30 40 50 60 MAVSVFSGVR LLSIGDANGD IQRHSEQQPL RLEIKMNQDA AQIILSNNEE TCVFKCTVLR 70 80 90 100 110 120 ETECSRVGKQ SFIITLGCNS VLLQFASPAD FSSFYNLLKI CRGQKGDRSV FSDRTEESSA 130 140 150 160 170 180 VQYFQFYGYL SQQQNMMQDY VRTGTYQRAI LQNHTDFKDK VVLDVGCGSG ILSFFAAQAG 190 200 210 220 230 240 ARKVYAVEAS TMAQHAEVLV NSNRLSERVV VIPGKVEEVS LPEQVDIIIS EPMGYMLFNE 250 260 270 280 290 300 RMLESYLHAK KFLKPSGKMF PTIGDVHLAP FTDEQLYMEQ FTKANFWYQP SFHGVDLSAL 310 320 330 340 350 360 RGAAVDEYFR QPIVDTFDIR ILMAKSVKYT VNFLEAKEED LYKIEIPFKF HMMHSGLVHG 370 380 390 400 410 420 LAFWFDVAFI GSVMTVWLST APTEPLTHWY QVRCLLQSPL FAKAGDTMSG TALLIANKRQ 430 440 450 460 470 480 SYDISIVAQV DQTGSKSSNL LDLKNPFFRY TGTTPAPPPG SHYSSPSENM WNTGGTYSMS 490 500 510 520 530 540 QGMAVSGMPT AYDLSTVIGG SGTTVSHNNL IPLGTDTHAL NTGIVNHTHS RMGSIMSTGI 550 560 570 580 VQGATTAQQG PSSASLHYPV TNQFTMGGPA ISMASPMAIP SNTMHYGS

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX649502 Genomic DNA. Translation: CAI20831.1. BC078292 mRNA. Translation: AAH78292.1.
IPI	IPI00481222.
RefSeq	NP_001003645.1.
UniGene	Dr.83746
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q6DC04.
Proteomic databases
PRIDE	Q6DC04.
Genome annotation databases
Ensembl	ENSDART00000006091; ENSDARP00000016959; ENSDARG00000018698; Danio rerio. [Genome view]
GeneID	445251.
KEGG	dre:445251.
Organism-specific databases
CTD	445251.
ZFIN	ZDB-GENE-040724-77. carm1.
Phylogenomic databases
HOVERGEN	Q6DC04.
Enzyme and pathway databases
BRENDA	2.1.1.125. 96826.
Gene expression databases
Bgee	Q6DC04.
Family and domain databases
InterPro	IPR010456. PrmA_MeTrfase. [Graphical view]
Pfam	PF06325. PrmA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARM1_DANRE

Accession

Primary (citable) accession number: Q6DC04

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	August 16, 2004
Last modified:	September 22, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project