Reviewed, UniProtKB/Swiss-Prot Q6DB95 (ASNA_ERWCT)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	ECA0003

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000017945

Sequences

	Sequence		Length	Mass (Da)
	Q6DB95-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 69F9D8FE12109BDF	FASTA	330	37,023
	10 20 30 40 50 60 MKKQYIEKQQ QISFVKSFFS SQLEQLLGLI EVQAPILSRI GDGTQDNLSG TEKAVQVKVK 70 80 90 100 110 120 ALPDATFEVV HSLAKWKRKT LGAYDFSFGE GIYTHMKALR PDEDRLSPIH SVYVDQWDWE 130 140 150 160 170 180 RVMEDGERNA EYLKSTVTRI YQGIKATEAA VHQAFGIQPF LPEQIHFVHT ETLLKRYPDL 190 200 210 220 230 240 DAKGRERAIA KELGAVFLIG IGGKLSSGHS HDVRAPDYDD WTTPGEQELA GLNGDIVVWN 250 260 270 280 290 300 PVLNDAFEIS SMGIRVDAEA LTRQLALTQD EERLKLEWHQ ALLRGEMPQT IGGGIGQSRL 310 320 330 VMLLLQLPHI GQVQCGVWPQ PLRESVSGLL

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG72927.1.
RefSeq	YP_048135.1.
3D structure databases
HSSP	HSSP built from PDB template 12AS based on UniProtKB P00963.
SMR	Q6DB95. Positions 5-330.
ModBase	Search...
Genome annotation databases
GeneID	2884104.
GenomeReviews	Gene locus ECA0003 in contig BX950851_GR.
KEGG	eca:ECA0003.
NMPDR	fig|218491.3.peg.4373.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA0003-MONOMER.
BRENDA	6.3.1.1. 281579.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. PF00152. tRNA-synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_ERWCT

Accession

Primary (citable) accession number: Q6DB95

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 16, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)