Reviewed, UniProtKB/Swiss-Prot Q6DB24 (GHRB_ERWCT)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase B
    EC=1.1.1.79
    EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	ECA0078

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01667
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667
Subunit structure	Homodimer By similarity. HAMAP MF_01667
Subcellular location	Cytoplasm Probable HAMAP MF_01667.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667		PRO_0000348382
Sites
	Active site	233	1	By similarity
	Active site	262	1	By similarity
	Active site	281	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DB24-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: C9DD8C744D103A03	FASTA	320	34,549
	10 20 30 40 50 60 MKPSVILYKK VADDLRARLD QHFTVTELDA FPALDHPALA TAEGIIGSGG KVDKDFLQHA 70 80 90 100 110 120 PRLRAASTIS VGYDTFNVDA LNEKGVILMH TPTVLTETVA DTVLALMLAS ARRVVEVVER 130 140 150 160 170 180 VKAGEWKGGV GSDWFGTDVH HKTIGILGMG RIGLAVAQRA HFGFSMPVLY NARRHHAEAE 190 200 210 220 230 240 ERFNARHCDL DTLLAESDFL CITLPLTAET HHLIGREQLA KMKPSAILIN IGRGAVVDED 250 260 270 280 290 300 ALTEALVKGT IQAAGLDVFV KEPLPVDSPL LDLPNVVALP HIGSATHETR YDMAACAVDN 310 320 LIAALSGQVK ENCVNPQVLK

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG72998.1. Different initiation.
RefSeq	YP_048206.2.
3D structure databases
HSSP	HSSP built from PDB template 2DBZ based on UniProtKB O58320.
ModBase	Search...
Genome annotation databases
GeneID	2884280.
GenomeReviews	Gene locus ECA0078 in contig BX950851_GR.
KEGG	eca:ECA0078.
NMPDR	fig|218491.3.peg.3040.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG731446.
OMA	KSIGPDW.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA0078-MONOMER.
Family and domain databases
HAMAP	MF_01667. 2-Hacid_dh_C_GhrB. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_ERWCT

Accession

Primary (citable) accession number: Q6DB24

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 19, 2010
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)