Reviewed, UniProtKB/Swiss-Prot Q6DB05 (XYLA_ERWCT)

Names and origin

Protein names

Recommended name:
    Xylose isomerase
    EC=5.3.1.5

Gene names

Name:	xylA
Ordered Locus Names:	ECA0097

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose. HAMAP MF_00455
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the xylose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xylose isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Xylose isomerase HAMAP MF_00455		PRO_0000236961
Sites
	Active site	101	1	By similarity
	Active site	104	1	By similarity
	Metal binding	232	1	Magnesium 1 By similarity
	Metal binding	268	1	Magnesium 1 By similarity
	Metal binding	268	1	Magnesium 2 By similarity
	Metal binding	271	1	Magnesium 2 By similarity
	Metal binding	296	1	Magnesium 1 By similarity
	Metal binding	307	1	Magnesium 2 By similarity
	Metal binding	309	1	Magnesium 2 By similarity
	Metal binding	339	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DB05-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: DBF1B45A7F60729A	FASTA	439	49,369
	10 20 30 40 50 60 MQAYFEQIEK VRYEGSQSSN PFAFRHYNPD QEILGKRMAD HLRFAVAYWH TFCWNGSDMF 70 80 90 100 110 120 GVGSFARPWQ QSGDALELAK RKADIAFEFF QKLSVPYYCF HDVDVAPEGN SLKEYLHNIA 130 140 150 160 170 180 VITDVLAEKQ QDSGVKLLWG TANCFTNPRY GAGAATNPDP DVFAWAATQV FTAMNATKTL 190 200 210 220 230 240 GGENYVLWGG REGYETLLNT DLRQEREQIG RFMQMVVEHK HKIGFQGTLL IEPKPQEPTK 250 260 270 280 290 300 HQYDYDVATV YGFLKQFGLE KEIKVNVEAN HATLAGHSFH HEIATAVALG VFGSVDANRG 310 320 330 340 350 360 DPQLGWDTDQ FPNSVEENTL IMYEILKAGG FTTGGLNFDA KVRRQSTDRY DLFHAHIGAM 370 380 390 400 410 420 DTMALALKAA ARMIEDDKLN QLVAKRYAGW NGELGQQILQ GNASLESLAQ YAESHQLAPQ 430 HQSGQQELLE NLVNRHLFG

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG73017.1.
RefSeq	YP_048225.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2882641.
GenomeReviews	Gene locus ECA0097 in contig BX950851_GR.
KEGG	eca:ECA0097.
NMPDR	fig|218491.3.peg.3059.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6DB05.
OMA	QFLIEPK.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA0097-MON.
BRENDA	5.3.1.5. 281579.
Family and domain databases
HAMAP	MF_00455. [Tree]
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR012307. Xyl_isomerase_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. IPR018115. Xylose_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
TIGRFAMs	TIGR02630. xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_ERWCT

Accession

Primary (citable) accession number: Q6DB05

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)