Q6DAY3 (LLDD_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase [cytochrome]
EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	ECA0119

Organism

Erwinia carotovora subsp. atroseptica (strain SCRI 1043 / ATCC BAA-672) (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

218491 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium › Erwinia carotovora subsp. atroseptica

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP-Rule MF_01559
Cofactor	FMN By similarity.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lactate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	L-lactate dehydrogenase [cytochrome] HAMAP-Rule MF_01559		PRO_0000206334
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6DAY3 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: F4AC9675731D720D	FASTA	386	41,720
	10 20 30 40 50 60 MIISASTDYR AAAQRRLPPF LFHYIDGGAY GEHTLRRNTA DLADIALRQR ILKNMSDLSL 70 80 90 100 110 120 ETQLFGEKLA MPVVLAPVGL TGMYARRGEV QAARAAAQKG IPFTLSTVSV CPIEEVAPAI 130 140 150 160 170 180 DRPMWFQLYV LKDRGFMRSA LERAQAAGVK TLVFTVDMPT PGARYRDAHS GMSGPNAAAR 190 200 210 220 230 240 RMLQAVTHPQ WAWDVGLNGK PHDLGNVSAY RGKPTTLEDY IGWLAANFDP SISWQDLAWI 250 260 270 280 290 300 REFWKGPMII KGILDPEDAK EAVRFGADGI VVSNHGGRQL DGVLSTAHAL PAIADAVKGD 310 320 330 340 350 360 ITILADSGIR SGLDVVRMIA LGADGVMLGR AFVYALAAAG EAGVVNLLNL IEKEMRVAMT 370 380 LTGAKSIADI TSDSLVQVTQ RRLDGL

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG73039.1.
RefSeq	YP_048247.1. NC_004547.2.
3D structure databases
ProteinModelPortal	Q6DAY3.
ModBase	Search...
Protein-protein interaction databases
STRING	218491.ECA0119.
Proteomic databases
PRIDE	Q6DAY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG73039; CAG73039; ECA0119.
GeneID	2883630.
KEGG	eca:ECA0119.
PATRIC	20475389. VBIPecAtr54885_0122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1304.
HOGENOM	HOG000217464.
KO	K00101.
OMA	EGPEMSL.
ProtClustDB	PRK11197.
Enzyme and pathway databases
BioCyc	PATR218491:GJNB-121-MONOMER.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01559. L-lact_dehydr.
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha-hydoxy_acid_DH_FMN. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. IPR020920. L-lactate_DHase_cyt. [Graphical view]
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_ERWCT

Accession

Primary (citable) accession number: Q6DAY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program