Reviewed, UniProtKB/Swiss-Prot Q6DAY3 (LLDD_ERWCT)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase [cytochrome]
    EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	ECA0119

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H(+).
Cofactor	FMN By similarity.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	L-lactate dehydrogenase [cytochrome]		PRO_0000206334
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6DAY3-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: F4AC9675731D720D	FASTA	386	41,720
	10 20 30 40 50 60 MIISASTDYR AAAQRRLPPF LFHYIDGGAY GEHTLRRNTA DLADIALRQR ILKNMSDLSL 70 80 90 100 110 120 ETQLFGEKLA MPVVLAPVGL TGMYARRGEV QAARAAAQKG IPFTLSTVSV CPIEEVAPAI 130 140 150 160 170 180 DRPMWFQLYV LKDRGFMRSA LERAQAAGVK TLVFTVDMPT PGARYRDAHS GMSGPNAAAR 190 200 210 220 230 240 RMLQAVTHPQ WAWDVGLNGK PHDLGNVSAY RGKPTTLEDY IGWLAANFDP SISWQDLAWI 250 260 270 280 290 300 REFWKGPMII KGILDPEDAK EAVRFGADGI VVSNHGGRQL DGVLSTAHAL PAIADAVKGD 310 320 330 340 350 360 ITILADSGIR SGLDVVRMIA LGADGVMLGR AFVYALAAAG EAGVVNLLNL IEKEMRVAMT 370 380 LTGAKSIADI TSDSLVQVTQ RRLDGL

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG73039.1.
RefSeq	YP_048247.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2883630.
GenomeReviews	Gene locus ECA0119 in contig BX950851_GR.
KEGG	eca:ECA0119.
NMPDR	fig|218491.3.peg.3169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6DAY3.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA0119-MON.
Family and domain databases
HAMAP	MF_01559. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha_OHA_DHase_FMN. IPR008259. FMN_hydac_DHase_AS. IPR000262. FMN_OHA_DHase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_ERWCT

Accession

Primary (citable) accession number: Q6DAY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	August 16, 2004
Last modified:	November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)