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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. dCTP deaminase (dcd)
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateUniRule annotation1
Binding sitei98Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Ordered Locus Names:ECA0143
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001828611 – 152Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST152

Proteomic databases

PRIDEiQ6DAV9.

Interactioni

Protein-protein interaction databases

STRINGi218491.ECA0143.

Structurei

3D structure databases

ProteinModelPortaliQ6DAV9.
SMRiQ6DAV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni71 – 73Substrate bindingUniRule annotation3
Regioni88 – 90Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108Z1K. Bacteria.
COG0756. LUCA.
HOGENOMiHOG000028968.
KOiK01520.
OMAiGVILINH.
OrthoDBiPOG091H01I6.

Family and domain databases

CDDicd07557. trimeric_dUTPase. 1 hit.
HAMAPiMF_00116. dUTPase_bact. 1 hit.
InterProiView protein in InterPro
IPR029054. dUTPase-like.
IPR036157. dUTPase-like_sf.
IPR008181. dUTPase_1.
IPR033704. dUTPase_trimeric.
PfamiView protein in Pfam
PF00692. dUTPase. 1 hit.
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6DAV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKKIDVKIV DPRVGQQFPL PTYATPGSAG LDLRACLDQA IELKAGETTL
60 70 80 90 100
IPTGLAIHIA DTGLAAVILP RSGLGHKHGV VLGNLVGLID SDYQGQLMVS
110 120 130 140 150
VWNRGQQTFT VEPGERIAQM VFVPVVQAEF NLVEDFVGSE RGEGGFGHSG

RS
Length:152
Mass (Da):16,194
Last modified:August 16, 2004 - v1
Checksum:iBE83B03A582D9282
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG73063.1.
RefSeqiWP_010285710.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG73063; CAG73063; ECA0143.
KEGGieca:ECA0143.

Similar proteinsi

Entry informationi

Entry nameiDUT_PECAS
AccessioniPrimary (citable) accession number: Q6DAV9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: August 16, 2004
Last modified: October 25, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families