Reviewed, UniProtKB/Swiss-Prot Q6DAJ6 (CAH_ERWCT)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase
    EC=4.2.1.1
Alternative name(s):
    Carbonate dehydratase

Gene names

Name:	cah
Ordered Locus Names:	ECA0257

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc By similarity.
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 244	225	Carbonic anhydrase		PRO_0000042160
Sites
	Metal binding	109	1	Zinc; catalytic By similarity
	Metal binding	111	1	Zinc; catalytic By similarity
	Metal binding	128	1	Zinc; catalytic By similarity
Amino acid modifications
	Disulfide bond	46 ↔ 199		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6DAJ6-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: BAAC954DDD4EC1B1	FASTA	244	26,664
	10 20 30 40 50 60 MKGKFSIALM LSACFSASAS DSVHWGYEGS GDPAHWGKLS PDFSLCETGK NQSPVNIQQA 70 80 90 100 110 120 LNAQHDPLQL AFQSGTQQII NNGHTVQVNV SSGNTLLLDN ETFALQQFHF HAPSENEIDG 130 140 150 160 170 180 KQFPLEGHFV YKNADGALTV IALMFQEGAA NPQLATAWQQ IPAHVDQAED VRTPIAIQAL 190 200 210 220 230 240 LPTSLNYYRF SGSLTTPPCS EGIRWLVLDH PVTASAEQIN QFSSVMHHAN NRPIQPLNGR IIIH

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX950851 Genomic DNA. Translation: CAG73177.1.
RefSeq	YP_048384.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2881347.
GenomeReviews	Gene locus ECA0257 in contig BX950851_GR.
KEGG	eca:ECA0257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6DAJ6.
OMA	QFHFHAP.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA0257-MON.
BRENDA	4.2.1.1. 281579.
Family and domain databases
InterPro	IPR001148. Carbonic_anhydrase_a-class_cat. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018340. Carbonic_anhydrase_CAH1-like. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
PANTHER	PTHR18952:SF2. Carbonic_anhydrase_CAH1-like. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
ProDom	PD000865. Euk_COanhd. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH_ERWCT

Accession

Primary (citable) accession number: Q6DAJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	August 16, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)