Reviewed, UniProtKB/Swiss-Prot Q6D9I7 (PLSB_ERWCT)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	ECA0628

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 825	825	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000049432
Regions
	Motif	306 – 311	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	Q6D9I7-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 4CD1B8F2E4FF8DFE	FASTA	825	93,499
	10 20 30 40 50 60 MSGWRKIYYK LLNLPLKLLV KSKVIPADPV VELGLDPSRP ILYVLPYNSQ ADLLTLRAKC 70 80 90 100 110 120 LALGLPDPSQ SFEFNGVELP SHVFINDGPR VFRYYVPKQK SVKLFHDYLD LHRANPDLDV 130 140 150 160 170 180 QMVPVSVMFG RSPGREGHTQ AAPHLRLLNG IEKFFAVLWL GRDSFVRFSS PVSLRYMATE 190 200 210 220 230 240 HGTDKTIAHK LARVARMHFS RQRLAAVGPR LPVRQELFNK LLDSKAIKKA VDDEARSKKI 250 260 270 280 290 300 SHEKAQQNAI ALMEEIAADF SYEAVRLSDR VLSWTWNRLY QGINVHNAER VRQLAQDGHG 310 320 330 340 350 360 IVYVPCHRSH MDYLLLSYVL YHQGLVPPHI AAGINLNFWP AGPIFRRLGA FFIRRTFKGN 370 380 390 400 410 420 KLYSTIFREY LGELFARGYS VEYFMEGGRS RTGRLLDPKT GTLAMTIQAM LRGGTRPITL 430 440 450 460 470 480 VPIYVGYEHV MEVGTYAKEL RGAVKEKEGF MQMVRGLRKL RNLGQGYVNF GEPLPLTTYL 490 500 510 520 530 540 NQHVPQWRDA IDPIEAQRPS WLTPTVQDIS MDIMVRINNS AAANAMNLCS TALLASRQRS 550 560 570 580 590 600 LTREQMHEQL DCYLQLLRQV PYHKDITAPK KTADELLEHA LGMNKFEVEK DSIGDIIILP 610 620 630 640 650 660 REQAVLMTYY RNNIQHLLIL PSLIASIVIH HRRITLAEVV RQITLIYPLL QAELFLHYSN 670 680 690 700 710 720 EQLPRVLETL ANELTRQELL CSRDGQLAIN PPRIRTLQLL SAGVRETLQR YAITLSLLCA 730 740 750 760 770 780 NPEINRGTLE KESRNMAQRL SVLHGINAPE FFDKAVFSTL VATLRTEGYI TDSAEAAQGD 790 800 810 820 IVTIYNILGD LITPEVRLTI ESASSSTEME ASTSSSQTAE ETTQG

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG73543.1.
RefSeq	YP_048744.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2883490.
GenomeReviews	Gene locus ECA0628 in contig BX950851_GR.
KEGG	eca:ECA0628.
NMPDR	fig|218491.3.peg.2975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG296590.
OMA	WNKLYQG.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA0628-MONOMER.
BRENDA	2.3.1.15. 281579.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_ERWCT

Accession

Primary (citable) accession number: Q6D9I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)