Reviewed, UniProtKB/Swiss-Prot Q6D9G1 (RLMG_ERWCT)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	ECA0654

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366453

Sequences

	Sequence		Length	Mass (Da)
	Q6D9G1-1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: FAC273D272A83842	FASTA	379	42,339
	10 20 30 40 50 60 MSQLELETCN LTLVRYPQVA ENSALQAWDA ADEYLLRELS TMEIAPGPRL IFNDTFGALA 70 80 90 100 110 120 CGLQAQSPVS ISDSYLSQLA TRHNLELNGY DADAVTLLDS MADLPDAPAL VVMRIPKTIA 130 140 150 160 170 180 LLEHQLRMLR KVVTPQTRII AGAKARDIHT STLQLFERVM GPTKTSLAWK KARLVHCEWA 190 200 210 220 230 240 ELKVSEQSLT TEWELDGYGY RIQNHANVYS RNGLDIGARF FMQHLPEQID GKIIDLGCGN 250 260 270 280 290 300 GVIGLAALEA NPDATVGFFD ESYMAVASSQ MNVEVNRPQD VERCSFVVNN GLSRVRRDTL 310 320 330 340 350 360 QAVLCNPPFH QLQAVTDEIA WQMFMDARRC LQVGGELRIV GNRHLDYFHK LKRLFGNCET 370 IASNTKFAVL RAVKTNSSR

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG73569.1.
RefSeq	YP_048770.1.
3D structure databases
SMR	Q6D9G1. Positions 27-373.
ModBase	Search...
Genome annotation databases
GeneID	2881605.
GenomeReviews	Gene locus ECA0654 in contig BX950851_GR.
KEGG	eca:ECA0654.
NMPDR	fig|218491.3.peg.3074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG321931.
OMA	CGNGVLG.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA0654-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_ERWCT

Accession

Primary (citable) accession number: Q6D9G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	August 16, 2004
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)