Q6D9D1 (MDH_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	ECA0685

Organism

Erwinia carotovora subsp. atroseptica (strain SCRI 1043 / ATCC BAA-672) (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

218491 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium › Erwinia carotovora subsp. atroseptica

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01516
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Malate dehydrogenase HAMAP-Rule MF_01516		PRO_0000113304
Regions
	Nucleotide binding	7 – 13	7	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	227	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6D9D1 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 1FD9CAE656E8E371	FASTA	311	32,273
	10 20 30 40 50 60 MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP TAVKIKGYSG 70 80 90 100 110 120 EDAKPALAGA DIVLISAGVA RKPGMDRSDL FNVNAGIVRN LVEQIAITCP KACIGIITNP 130 140 150 160 170 180 VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD IIRSNTFVAE LKGKQPQDIN VPVIGGHSGV 190 200 210 220 230 240 TILPLLSQVS GISFSEQEVA DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR 250 260 270 280 290 300 ALQGENGVVE CAYVESDGKH ARFFAQPILL GKDGVAERKD IGTLSAFEQN ALSSMLDTLK 310 QDIELGETFI K

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG73599.1.
RefSeq	YP_048800.1. NC_004547.2.
3D structure databases
ProteinModelPortal	Q6D9D1.
SMR	Q6D9D1. Positions 1-310.
ModBase	Search...
Protein-protein interaction databases
STRING	218491.ECA0685.
Proteomic databases
PRIDE	Q6D9D1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAG73599; CAG73599; ECA0685.
GeneID	2885116.
KEGG	eca:ECA0685.
PATRIC	20476542. VBIPecAtr54885_0681.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000213792.
KO	K00024.
OMA	NVIECSY.
ProtClustDB	PRK05086.
Enzyme and pathway databases
BioCyc	PATR218491:GJNB-702-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01516. Malate_dehydrog_1.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR023958. Malate_DH_type1_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_ERWCT

Accession

Primary (citable) accession number: Q6D9D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	August 16, 2004
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program