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Q6D9A1

- PNP_PECAS

UniProt

Q6D9A1 - PNP_PECAS

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi486 – 4861MagnesiumUniRule annotation
Metal bindingi492 – 4921MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPATR218491:GJNB-738-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:ECA0716
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium
ProteomesiUP000007966: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Polyribonucleotide nucleotidyltransferasePRO_0000329637Add
BLAST

Proteomic databases

PRIDEiQ6D9A1.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA0716.

Structurei

3D structure databases

ProteinModelPortaliQ6D9A1.
SMRiQ6D9A1. Positions 617-692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini553 – 61260KHUniRule annotationAdd
BLAST
Domaini622 – 69069S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6D9A1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLNPIVRKFQ YGQHTVTLET GMMARQATAA VMVSMDDTAV FVTVVGAKNA
60 70 80 90 100
KPGQSFFPLT VNYQERTYAA GRFPGGFFRR EGRPSEGETL TSRLIDRPIR
110 120 130 140 150
PLFPEGFLNE VQVIATVVSV NPQVSPDIVA MIGASAALSL SGIPFSGPIG
160 170 180 190 200
VARVGYLNDQ YVLNPTTDEL KESRLDLVVA GTQGAVLMVE SEAELLSEDQ
210 220 230 240 250
MLGAVVFGHE QQQIVIENIN SLVAEAGKPK WEWHAPAVNV SLEQRVQALS
260 270 280 290 300
EARLGDAYRI TEKQERYAQV NVIKTDVVAA LQAEDETLNA GEIQEILGNI
310 320 330 340 350
EKNVVRGRVL AGEPRIDGRE KDMIRGLDVR TGVLPRTHGS ALFTRGETQA
360 370 380 390 400
LVTATLGTER DAQNIDELTG ERTDRFLLHY NFPPYCVGET GMVGSPKRRE
410 420 430 440 450
IGHGRLAKRG VLAVMPKANE FPYTVRVVSE ITESNGSSSM ASVCGASLAL
460 470 480 490 500
MDAGVPIKSA VAGIAMGLVK EGDNFVVLSD ILGDEDHLGD MDFKVAGSRE
510 520 530 540 550
GITALQMDIK IEGITREIMQ VALNQAKGAR LHILGVMEQA ISTPRGDISE
560 570 580 590 600
FAPRIHTIKI STDKIKDVIG KGGSVIRALT EETGTTIEIE DDGTVRIAST
610 620 630 640 650
DGEKAKHAIR RIEEITAEIE VGRVYQGKVT RIVDFGAFVA IGGGKEGLVH
660 670 680 690 700
ISQIADKRVE KVTDYLQMGQ EVPVKVLEVD RQGRVRLSIK EANPQTQEAA

APSSEE
Length:706
Mass (Da):76,485
Last modified:August 16, 2004 - v1
Checksum:i01D440A633E7849C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG73631.1.
RefSeqiYP_048830.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG73631; CAG73631; ECA0716.
GeneIDi2883240.
KEGGieca:ECA0716.
PATRICi20476612. VBIPecAtr54885_0714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG73631.1 .
RefSeqi YP_048830.1. NC_004547.2.

3D structure databases

ProteinModelPortali Q6D9A1.
SMRi Q6D9A1. Positions 617-692.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 218491.ECA0716.

Proteomic databases

PRIDEi Q6D9A1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAG73631 ; CAG73631 ; ECA0716 .
GeneIDi 2883240.
KEGGi eca:ECA0716.
PATRICi 20476612. VBIPecAtr54885_0714.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci PATR218491:GJNB-738-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCRI 1043 / ATCC BAA-672.

Entry informationi

Entry nameiPNP_PECAS
AccessioniPrimary (citable) accession number: Q6D9A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 16, 2004
Last modified: November 26, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3