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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi486MagnesiumUniRule annotation1
Metal bindingi492MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:ECA0716
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003296371 – 706Polyribonucleotide nucleotidyltransferaseAdd BLAST706

Proteomic databases

PRIDEiQ6D9A1.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA0716.

Structurei

3D structure databases

ProteinModelPortaliQ6D9A1.
SMRiQ6D9A1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini553 – 612KHUniRule annotationAdd BLAST60
Domaini622 – 690S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRYMHNYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6D9A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNPIVRKFQ YGQHTVTLET GMMARQATAA VMVSMDDTAV FVTVVGAKNA
60 70 80 90 100
KPGQSFFPLT VNYQERTYAA GRFPGGFFRR EGRPSEGETL TSRLIDRPIR
110 120 130 140 150
PLFPEGFLNE VQVIATVVSV NPQVSPDIVA MIGASAALSL SGIPFSGPIG
160 170 180 190 200
VARVGYLNDQ YVLNPTTDEL KESRLDLVVA GTQGAVLMVE SEAELLSEDQ
210 220 230 240 250
MLGAVVFGHE QQQIVIENIN SLVAEAGKPK WEWHAPAVNV SLEQRVQALS
260 270 280 290 300
EARLGDAYRI TEKQERYAQV NVIKTDVVAA LQAEDETLNA GEIQEILGNI
310 320 330 340 350
EKNVVRGRVL AGEPRIDGRE KDMIRGLDVR TGVLPRTHGS ALFTRGETQA
360 370 380 390 400
LVTATLGTER DAQNIDELTG ERTDRFLLHY NFPPYCVGET GMVGSPKRRE
410 420 430 440 450
IGHGRLAKRG VLAVMPKANE FPYTVRVVSE ITESNGSSSM ASVCGASLAL
460 470 480 490 500
MDAGVPIKSA VAGIAMGLVK EGDNFVVLSD ILGDEDHLGD MDFKVAGSRE
510 520 530 540 550
GITALQMDIK IEGITREIMQ VALNQAKGAR LHILGVMEQA ISTPRGDISE
560 570 580 590 600
FAPRIHTIKI STDKIKDVIG KGGSVIRALT EETGTTIEIE DDGTVRIAST
610 620 630 640 650
DGEKAKHAIR RIEEITAEIE VGRVYQGKVT RIVDFGAFVA IGGGKEGLVH
660 670 680 690 700
ISQIADKRVE KVTDYLQMGQ EVPVKVLEVD RQGRVRLSIK EANPQTQEAA

APSSEE
Length:706
Mass (Da):76,485
Last modified:August 16, 2004 - v1
Checksum:i01D440A633E7849C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA. Translation: CAG73631.1.
RefSeqiWP_011092325.1. NC_004547.2.

Genome annotation databases

EnsemblBacteriaiCAG73631; CAG73631; ECA0716.
KEGGieca:ECA0716.
PATRICifig|218491.5.peg.714.

Similar proteinsi

Entry informationi

Entry nameiPNP_PECAS
AccessioniPrimary (citable) accession number: Q6D9A1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: August 16, 2004
Last modified: August 30, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families