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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei94S-adenosyl-L-methionineUniRule annotation1
Binding sitei121S-adenosyl-L-methionineUniRule annotation1
Active sitei144By similarity1
Binding sitei144S-adenosyl-L-methionineUniRule annotation1
Binding sitei148SubstrateUniRule annotation1
Binding sitei180SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPATR218491:G1G3T-1012-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:ECA0977
OrganismiPectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
Taxonomic identifieri218491 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000007966 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713301 – 239tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST239

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi218491.ECA0977

Structurei

3D structure databases

ProteinModelPortaliQ6D8J7
SMRiQ6D8J7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 155Interaction with RNAUniRule annotation6
Regioni217 – 220Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q6D8J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINNVISPEF DENGRPMRRI RSFVRRQGRL TNGQQLALDN YWPVMGVEYQ
60 70 80 90 100
TEPVDFNALF GRDAPVVLEI GFGMGASLVT MAAQHPEQNF LGIEVHLPGV
110 120 130 140 150
GACLASAQDV EISNLRVMCH DALEVLMKMI PDSSLSMVQL FFPDPWHKVR
160 170 180 190 200
HNKRRIVQAP FVELVQSKLK VGGVFHMATD WEPYARHMLK VMTSVAGYRN
210 220 230
LSDNNEYVVR PESRPLTKFE ARGQRLGHGV WDLMFERIK
Length:239
Mass (Da):27,248
Last modified:August 16, 2004 - v1
Checksum:i08E3CAA1804CB7D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX950851 Genomic DNA Translation: CAG73888.1
RefSeqiWP_011092577.1, NC_004547.2

Genome annotation databases

EnsemblBacteriaiCAG73888; CAG73888; ECA0977
KEGGieca:ECA0977
PATRICifig|218491.5.peg.983

Similar proteinsi

Entry informationi

Entry nameiTRMB_PECAS
AccessioniPrimary (citable) accession number: Q6D8J7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 16, 2004
Last modified: February 28, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome