Q6D8H5 (ARGA_ERWCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amino-acid acetyltransferase
EC=2.3.1.1

Alternative name(s):
N-acetylglutamate synthase
Short name=AGS
Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	ECA0999

Organism

Erwinia carotovora subsp. atroseptica (Pectobacterium atrosepticum) [Complete proteome] [HAMAP]

Taxonomic identifier

29471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subunit structure	Homohexamer By similarity. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity HAMAP MF_01105.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000084812
Regions
	Domain	295 – 434	140	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	Q6D8H5 [UniParc]. Last modified August 16, 2004. Version 1. Checksum: 257E58677547E455	FASTA	441	49,073
	10 20 30 40 50 60 MKERSTELVQ GFRHSVPYIN AHRGKTFVIM LGGEAIEHAN FSSIVNDIGL LHSLGIKLVV 70 80 90 100 110 120 VYGARPQIDA NLTTHHYEPH YHKNTRITDS ATLELVKQAA GMLQLDITAR LSMSLNNTPL 130 140 150 160 170 180 QGAHINVVSG NFIIAQPLGV DDGVDYCHSG RIRRIDEEAV HRQLNSGAIV LLGPVAVSVT 190 200 210 220 230 240 GESFNLTSEE VATQLAIKLK AEKMIGFCSS QGVTNEEGRI ISELFPDDAQ QRIDVLEQAG 250 260 270 280 290 300 DYHSGTVRFL RGAVKACRSG VRRSHLISYQ DDGALLQELF SRDGIGTQIV MESAEQVRRA 310 320 330 340 350 360 TINDIGGILE LIRPLEEQGI LVRRSREQLE MEIDKFTVVV RDNLTIACAA LYPFPEESIG 370 380 390 400 410 420 EMACVAVHPD YRSSSRGDML LMRVAAQARQ QGLQKLFVLT THSIHWFQER GFLPAEVEML 430 440 PKKKQALYNY QRRSKILVLD L

References

[1]

"Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors." Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J., Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K., Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J., Fraser A., Hance Z. , Hauser H., Jagels K., Moule S., Norbertczak H., Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S., Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K. Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004) [PubMed: 15263089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCRI 1043 / ATCC BAA-672.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX950851 Genomic DNA. Translation: CAG73910.1.
RefSeq	YP_049106.1. NC_004547.2.
3D structure databases
HSSP	HSSP built from PDB template 2BUF based on UniProtKB Q9HTN2.
ProteinModelPortal	Q6D8H5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2884143.
GenomeReviews	Gene locus ECA0999 in contig BX950851_GR.
KEGG	eca:ECA0999.
NMPDR	fig|218491.3.peg.4114.
PATRIC	20477206. VBIPecAtr54885_1006.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG545264.
OMA	AFNLAME.
ProtClustDB	PRK05279.
Enzyme and pathway databases
BioCyc	ECAR218491:ECA0999-MONOMER.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K14682.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_ERWCT

Accession

Primary (citable) accession number: Q6D8H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	August 16, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program